International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Evaluation of Relative Fluoride Toxicity and Its Impact on Growth, Economic Characters and Fecundity of the Silkworm, Bombyx mori L.

  • Ramakrishna, S. (Department of Zoology, Bangalore University) ;
  • Nath, B.Surendra (Department of Zoology, Bangalore University) ;
  • Jayaprakash (Seribiotech Research Laboratory, CSB Campus, Carmelram Post)
  • Published : 2004.06.01
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Abstract

A detailed evaluation of relative toxicity of fluoride to die 5th instar larvae of PM and NB$_4$D$_2$ races of silkworm and the effects of lethal, sublethal and prevailing levels of fluoride in groundwater on the growth, economic characters and fecundity of the silkworm, Bombyx mori (Lepidoptera: Bombycidae) were studied. The feeding of mulberry, Morus alba leaves treated with lethal and sublethal doses of fluoride to PM and NB$_4$D$_2$ races of silkworms from the beginning of the 5th instar to the end of the feeding period resulted in significant reduction in growth, single cocoon weight, single shell weight, silk index, average filament length, and fecundity when compared to controls. These manges were more pronounced on exposure to lethal dose than sublethal dose and in general, the changes induced by fluoride were more striking in NB$_4$D$_2$ than PM, indicating the greater resistance of PM to higher fluoride levels. Groundwater quality in sericulturally important villages of Karnataka and Andhra Pradesh States has been studied with special reference to the presence of fluoride. On exposure to dose of prevailing levels in the waters of sericulturally important areas, die changes observed on growth, economic characters and fecundity were slightly lower when compared to controls and the decrease was found to be insignificant (P$\geq$0.05). It was concluded that, though minute dose (4.0 ppm) of fluoride did not have any toxic impact, it is toxic at higher concentrations to silkworms.

Keywords

References

  1. Aftab, A. C. A and M. V. Chandrakala (1999) Effect of oral administration of sodium fluoride on food and water utilization in silkworm, Bombyx mori L. Insect. Sci. Applica. 19, 193-198
  2. Aftab, A. C. A., M. V Chandrakala and V. G. Maribashetty (1999) Influence of sodium fluoride on nutritional efficiency of a muItivoItine race of the silkworm, Bombyx mori. J. Exp. Zool. India 2, 127-133
  3. Bhatnagar, M., P. Rao and S. Jain (2002) Neurotoxicity of fluoride: Neurodegeneration in hippocampus of female mice. Ind. J. Exp. Biol. 40, 546-554
  4. Bhosale, S. H., V. B. Yadwad and V. L. Kallapur (1988) Residual toxic effect of Ekalux EC-25 on the biochemical constituents of the fat body of 5th instar Bombyx mori. Ind. J. Seric. 27,73-77
  5. Chandrakala, M. V., C. Shivakumar and C. R. Mahadevaiah (1998) Effect of oral administration of sodium fluoride on survival rate and cocoon yield in some silkworm (Bombyx mori L.) Races. Ind. J. Ecol. 25, 165-170
  6. Chen, Y. Y. (1993) Changes in fluoride and calcium in the blood of the fluorosis-affected silkworm, Bombyx mori L. Sericologia 33, 153-157
  7. Chen, Y. Y. and Y. C. Wu (1994) Study on the effect of fluoride on growth, development and economic parameters of the silkworm, Bombyx mori. Sericologia 34, 627-635
  8. Chen, Y. Y. and Y. C. Wu (1995) Fluoride loading and kinetics in different tissues of larvae of fluorosis silkworm (Bombyx mori L.). Sericologia 34, 1-10
  9. Dasmahapatra, A. K., M. K. Chakrabarti and A. K. Medda (1989) Effect of potassium iodide, cobalt chloride, calcium chloride and potassium nitrate on protein, RNA and DNA contents of silk gland of silkworm, Bombyx mori L. Nistari race. Sericologia 29, 355-359
  10. Davies, I., A. W. Davison and G. R. Port (1992) Fluoride loading of larvae of pine sawfly from a polluted site. J. Appl. Ecol. 29, 63-69 https://doi.org/10.2307/2404348
  11. Finney, D. J. (1971) Probit analysis, 3rd edn. Cambridge University Press, London and New York. pp. 333
  12. Fujii, M. and S. Honda (1972) The relative oral toxicity of some fluorine compounds for silkworm larvae. J. Seric. Sci. Jpn 41, 104-110
  13. Gayathri, M. V. and N. B. Krishnamurthy (1979) Differential sensitivity of different species of Drosophila to ceresan- a mercurial fungicide. Ind. J. Exp. BioI. 17,433-435
  14. Gerking, S. D. (1952) The protein metabolism of the sunfishes of different ages. Physiol. Zool. 25, 358-372 https://doi.org/10.1086/physzool.25.4.30152132
  15. Harwood, J. E. (1969) The use of an ion selective electrode for routine analysis of water samples. Water. Res. 3, 273
  16. He, Y. T. (1984) A preliminary note on the effects of fluoride pollution in the atmosphere on silkworm cocoon production. Guangdong Seric. Bull. 1, 1-4
  17. Joshi, K. L. (1985) Studies on growth indices for eri silkworm, Philosamia ricini Hutt. (Lepidoptera: Satumiidae). Sericologia 25,313-319
  18. Krishnaswami, S. (1978) New technology of silkworm rearing. Cent. Seric. Res. Train. Inst. Bull. Mysore, India 2, 1-24
  19. Krishnaswami, S., M. N. Narasimhanna, S. K. Suryanarayana and S. Kumararaj (1973) Manual of Sericulture Silkworm Rearing. Agriculture Service Bulletin, F.A.O. Rome. pp. 64
  20. Kuribayashi, S. (1977) Effects of atmospheric pollution by hydrogen fluoride on mulberry tree and silkworm. J. Seric. Sci. Jpn 46, 536-544
  21. Kuwana, Z., S. Nakamura and H. Sugiyama (1967) Effects of insecticides on the silkworm larvae, Bombyx mori L. Analysis of the insecticide-silkworm larvae relationship by means of subcutaneous injection and topical and oral applications. Bull. Seric. Exp. Sta. 22, 123-180
  22. Luning, K. G. (1966) Drosophila tests in pharmacology. Nature 209, 84-86 https://doi.org/10.1038/209084a0
  23. Maynord, A. L. and K. J. Loosli (1962) Animal Nutrition, Mc Graw Hill, New York
  24. Muralidharan, D., A. P. Nair and U. Sathyanarayana (2002) Fluoride in shallow aquifers in Rajgarh Tehsil of Churu District, Rajasthan- an arid environment. Curr. Sci. 83, 699-702
  25. Nath, B. S. (1993) Studies on organophosphorus insecticide toxicity during 5th instar of silkworm, Bombyx mori L. Ph. D. Thesis, Sri Krishnadevaraya University, Anantapur, India
  26. Nath, B. S., A. Suresh, B. M. Varma and R. P. S. Kumar (1997) Changes in protein metabolism in hemolymph and fat body of the silkworm, Bombyx mori (Lepidoptera: Bombycidae), in response to organophosphorus insecticide toxicity. Ecotoxicol. Environ. Saf. 36, 169-173 https://doi.org/10.1006/eesa.1996.1504
  27. Nath, B. S. and R. P. S. Kumar (1999) Toxic impact of organophosphorus insecticides on acetylcholinesterase activity in the silkworm, Bombyx mori L. Ecotoxicol. Environ. Saf. 42, 157-162 https://doi.org/10.1006/eesa.1998.1735
  28. Nobbs, C. L. and D. W. Pearu (1976) The economics of stock pollutants. The example of cadmium. Int. Environ. Stu. 8, 245-255
  29. Pandian, T. J. and R. Raghuraman (1972) Effects of feeding rates on conversion efficiency and chemical composition of the fish Tilapia mossambica. Marine. Biol. 12, 127-136
  30. Perkin, E. J. (1979) Need for sublethal studies. Phil. Trans. R. Soc. London B. 286, 425-432
  31. Reddy, S. V., N. S. Reddy and R. Ramamurthi (1989) Carbaryl effects on the growth and silk qualities of the silkworm, Bombyx mori L. Ind. J. Seric. 28, 182-190
  32. Samaranayaka, M. (1974) Insecticide induced release of hyperglyaemic and adipokinetic hormones of Schistocerea gregaria. Gen. Comp. Endocrinol. 24, 424-428 https://doi.org/10.1016/0016-6480(74)90156-7
  33. Sankaranarayanan, K. (1969) The effect of nitrogen and oxygen frequency of X-ray induced dominant lethals and the physiology of the sperms in Drosophila melanogaster. Mut. Res. 4, 641-661
  34. Shong, L. F. (1985) A report on the effect of fluoride pollution on sericulture production. Jianshu Seric. 3, 16-19
  35. Shortt, H. E., G. R. Mc Robert, T. W. Barnard and M. Nayer (1937) Endemic fluorosis in Madras Presidency.Ind. J. Med. Res. 25, 553-555
  36. Stockner, J. G. and N. J. Anita (1976) Phytoplankton adaptation to environmental stresses from toxicants, nutrients and pollutants a warning. J. Fish. Res. Bd. Can. 33, 2089-2096 https://doi.org/10.1139/f76-260
  37. Susheela, A. K. (2001) A Treatise on Fluorosis. Fluorosis Research and Rural Development Foundation, Delhi, India. pp.15
  38. Valdiya, K. S. (1987) Environment Geology Indian Context, Tata Mc Graw Hill. New Delhi, India
  39. WHO (1984) Fluoride and fluorides, Environmental Health Criteria, World Health Organization, Geneva
  40. Wu, F. Z. (1990) Some problems on fluoride pollution of field by exhausting from brickklin. Acta. Agric. Universitatis Zhejiangensis 4, 48-52
  41. Wu, X., J. Xu and Cuiweizheng (1998) The activity of catalase in hemolymph of the silkworm, Bombyx mori L. and its relation to larval resistance. Acta. Entomol. Sinica 41, 124-129