• Title/Summary/Keyword: Farming activity

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Plant Growth Promotion and Antagonistic Activities Against Anthracnose of Burkholderia sp. LPN-2 Strain

  • Kim, WonChan;Seo, SangHyun;Lee, ChangHee;Park, JunHong;Kang, SangJae
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.3
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    • pp.251-258
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    • 2016
  • A rhizobacterium LPN-2, which showed strong antifungal activity and auxin producing ability, was isolated from a farmland in North Gyeongsang Province, South Korea. Based on analysis of the 16S rDNA sequence, strain LPN-2 was identified as a novel strain of Burkholderia and was designated as Burkholderia sp. LPN-2. In vitro experiments showed that the isolated stain LPN-2 significantly produced auxin within 48 hr incubation. In order to check for PGPR function we performed in vivo growth promoting test in different crops, including mung bean, pea and cabbage. Application of Burkholderia sp. LPN-2 showed dramatic growth promoting effect on all the tested plants. We also confirmed siderophore and cellulase productions by Burkholderia sp. LPN-2 using CAS blue agar and CMC plate test. Further treatment with LPN-2 and the crude culture broth was effective in suppressing anthracnose in vitro test and also reduced incidence and severity of anthracnose in apple and pepper. Taken together, we conclude that Burkholderia sp. LPN-2 might be used as organic fertilizer for effective crop production in organic farming.

Biological control of Botrytis cinerea on tomato using antagonistic bacteria

  • Hong, Sung-Jun;Kim, Yong-Ki;Jee, Hyeong-Jin;Park, Jong-Ho;Han, Eun-Jung;An, Nan-Hee;Kim, Jung-Hyun;Goo, Hyung-Jin
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.251-254
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    • 2011
  • Botrytis cinerea infects stems and leaves of greenhouse tomatoes and can cause serious economic losses. This study was conducted to develop environment-friendly control method against tomato gray mold. Antagonistic microorganisms (bacteria) were screened for control activity against Botrytis cinerea, both in vitro and in vivo, using stem sections. One hundred bacterial strains were isolated from the rhizospheric soil of various plants including tomato. These strains were screened for growth inhibition of Botrytis cinerea on agar plate by the dual culture and thirty strains showing strongly inhibitory effect against the pathogen were selected first. Among thirty strains, JB 5-12, JB 22-2, JB 22-3, U 4-8 and U46-6 reduced significantly disease incidence, when applied simultaneously with the pathogen. These results suggested that five antagonistic bacteria strains selected have the potential to control tomato gray mold in organic farming.

Immunosecurity: immunomodulants enhance immune responses in chickens

  • Yu, Keesun;Choi, Inhwan;Yun, Cheol-Heui
    • Animal Bioscience
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    • v.34 no.3_spc
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    • pp.321-337
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    • 2021
  • The global population has increased with swift urbanization in developing countries, and it is likely to result in a high demand for animal-derived protein-rich foods. Animal farming has been constantly affected by various stressful conditions, which can be categorized into physical, environmental, nutritional, and biological factors. Such conditions could be exacerbated by banning on the use of antibiotics as a growth promoter together with a pandemic situation including, but not limited to, African swine fever, avian influenza, and foot-and-mouth disease. To alleviate these pervasive tension, various immunomodulants have been suggested as alternatives for antibiotics. Various studies have investigated how stressors (i.e., imbalanced nutrition, dysbiosis, and disease) could negatively affect nutritional physiology in chickens. Importantly, the immune system is critical for host protective activity against pathogens, but at the same time excessive immune responses negatively affect its productivity. Yet, comprehensive review articles addressing the impact of such stress factors on the immune system of chickens are scarce. In this review, we categorize these stressors and their effects on the immune system of chickens and attempt to provide immunomodulants which can be a solution to the aforementioned problems facing the chicken industry.

An overview bioactive compounds on the skin of frogs (Anura)

  • Tran Thi Huyen;Phan Thi Hoang Anh;Nguyen Thi Anh Hong;Nguyen Ngoc Duyen;Le Pham Tan Quoc;Tran Dinh Thang
    • Fisheries and Aquatic Sciences
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    • v.26 no.4
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    • pp.241-255
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    • 2023
  • The robust development of frog farming offered high economic benefits but created a large waste residue of frog bones and skin that received little attention. Over the years, inedible by-products have often been processed into biomolecules of potential value and environmental benefits, such as collagen, gelatin, and bioactive peptides. An overview of bioactive compounds on frog skins from various countries indicated that brevinin was the most abundant biological peptide found in frog skin. Other remaining compounds also possessed their highlighted activities, including antibacterial, stimulating insulin release and gastric hormone release, anti-cancer, and neuroregulatory. Notably, various components have been analyzed in the structure and sequence to give meaningful insight into clustering components related to their biological activity. This review may create a source of raw materials for the developmental research of by-products from frog skin and concomitantly reduce environmental pollution.

Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces

  • Suphachai Tharavecharak;Corina N. D'Alessandro-Gabazza;Masaaki Toda;Taro Yasuma;Taku Tsuyama;Ichiro Kamei;Esteban C. Gabazza
    • Mycobiology
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    • v.51 no.2
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    • pp.94-108
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    • 2023
  • Termitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO2 conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a coadjuvant therapy in malignant diseases.

A Pilot Study for the Development of Functional Rural Workwear (기능성 농촌작업복 개발을 위한 예비연구)

  • Seungjung Na;Sujoung Cha
    • Journal of Fashion Business
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    • v.28 no.1
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    • pp.113-130
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    • 2024
  • A questionnaire survey of farmers engaged in actual farming was conducted to investigate the farmers' actual wearing conditions and preferences for agricultural workwear to develop workwear that is suitable for improved agricultural work efficiency. We extracted basic data necessary for the development of agricultural workwear. Analysis of the photographs taken showed differences in the shoes and gloves worn depending on the type of work. The percentages of men and women were equal among the agricultural workers. The age of the agricultural workers was analyzed and the results showed a higher percentage of those over 50 years old, indicating that the age of the agricultural workers was higher than in other industries. It was found that the waist, knees, and elbows of the workers were pulled during work, and analysis showed that the symptoms resulting from clothing pulling were more severe when the wearing dimensions were larger. It was recognized that the important aspects of workwear are those related to work, such as functionality, activity, and work efficiency. In addition to workwear, the most commonly worn auxiliary equipment was gloves, and other items included hats, armbands, work shoes, boots, seat chairs, and masks. When developing rural workwear, it is necessary to consider the fit, type of work, and materials of the workwear. Future research should focus on materials that can improve the functionality of workwear and pattern development that reflects physical and movement characteristics.

Comparative Analysis of the Antioxidant Activities of Green Peppers Cultivated under Conventional and Environmental-Friendly Farming conditions

  • Choi, Jang-Yeol;Choi, Yeo-Jin;Lee, Seong-Gene
    • Korean Journal of Environmental Agriculture
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    • v.29 no.1
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    • pp.39-46
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    • 2010
  • Environmental-friendly agriculture (EFA) is defined as the cultivation of crops with reduced amounts or without chemical-synthetic pesticides. Recently, the use of chemical pesticides has decreased significantly; therefore, we cultivated peppers following EFA- and conventional methods and compared their antioxidant activities. To accomplish this, the environmental-friendly cultivated peppers (EFPE) and conventionally cultivated peppers (CCPE) were extracted with 70% methanol and the effects of the extracts on the cell viability, intracellular ROS generation, lipid peroxidation and catalase activity of HepG2 cells were evaluated. EFPE showed a stronger protective effect against oxidative stress induced-cell death than that of CCPE. EFPE also reduced intracellular ROS generation (42.7% to 26.4%) following treatment with hydrogen peroxide more effectively than that of CCPE (24.2% to 6.3%). Furthermore, EFPE and CCPE showed protective effects against lipid peroxidation and induced catalase activity, although these effects were not statistically significant. Taken together, these results suggest that EFPE showed stronger antioxidant activities than CCPE, and thus represent evidence that EFA with biocontrol materials may improve the functional properties of crops and/or secondary metabolites with antioxidant activities when compared with conventional agricultural practices.

A Longitudinal Study on Seasonal Variation of Riboflavin Status of Rural Women : Dietary Intake, Erythrocyte Glutathione Reductase Activity Coefficient, and Urinary Riboflavin Excretion (식이섭취와 적혈구 Glutathione Reductase 활성도 및 소변배설에 근거한 일부 농촌여성들의 리보플라빈 영양상태에 관한 종단연구)

  • 임화재
    • Journal of Nutrition and Health
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    • v.29 no.5
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    • pp.507-516
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    • 1996
  • This study was undertaken to estimate seasonal variation of riboflavin status and investigate the relationship between riboflavin intake and its biochemical status in rural women. Dietary intake was measured by determining both 24hr recall method and conventient method. The riboflavin intake was also estimated by food frequency method. Ribofiavin biochemeical status was assessed by erythrocyte glutathione reductase activity coefficient(EGR AC) and urinary riboflavin excretion. All information was repeatly collected in three seasons ; farming season (June), harvest season(October), nonfarming season(February). Mean daily riboflavin intake was below RDA for Koreans in all seasons. Cereal & pulse, vegetable were the primary sources of riboflavin intake and provided above 60% of the total dietary riboflavin intake in all seasons. Riboflavin biochemical status was significantly different among seasons(EGR AC P<0.005, 24hr urinary riboflavin excretion P<0.05), and riboflavin biochemical deficiency was highest in February. The mean values of EGR AC revealed riboflavin deficiency in all seasons. However the mean values of urinary riboflavin excretion were within the normal range except 24hr urinary riboflavin excretion in February. On the basis of EGR AC criteria, 44.7% of subjects were at risk of deficiency in June, 44.7% in October, 81.6% in February. Result of individual riboflavin status assessed by EGR AC or 24hr urinary riboflavin excretion criteria was quite alike. Urinary riboflavin excretion per gram of creatinine underestimated riboflavin deficiency. Significant correlations were observed among biochemical indexes. It also appeared that riboflavin intake measured by food frequency method showed significant correlation with biochemical indexes, especially high correlation with EGR AC (P<0.001). The results indicated that the prevalence of riboflavin deficiency seems to be high among rural women in all seasons and the inadequacy of dietary riboflavin intake was reflected in the abnormalcy of riboflavin biochemical status assessed by EGR, AC. Therefore EGR AC seems to be more sensitive measure of impaired riboflavin status compared to urinary excretion.

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Effects of Dietary Supplementation with Ferulic Acid or Vitamin E Individually or in Combination on Meat Quality and Antioxidant Capacity of Finishing Pigs

  • Lia, Y.J.;Lia, L.Y.;Li, J.L.;Zhang, L.;Gao, F.;Zhou, G.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.3
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    • pp.374-381
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    • 2015
  • This study aimed to evaluate the effects of vitamin E (VE), ferulic acid (FA) and their combination supplementation on meat quality and antioxidant capacities of finishing pigs. Sixty barrows were randomly allocated to four experimental diets using a $2{\times}2$ factorial arrangement with 2 VE supplemental levels (0 or 400 mg/kg) and 2 FA supplemental levels (0 or 100 mg/kg) in basal diets. After 28 days, six pigs per treatment were slaughtered. The results showed that VE supplementation increased loin eye area of pigs (p<0.05) and FA supplementation increased $pH_{45min}$ value (p<0.05). The interaction of $FA{\times}VE$ was observed in shear force of longissimus dorsi muscle (p<0.05). Moreover, supplementation with VE decreased hepatic and sarcous malondialdehyde (MDA) content, increased hepatic glutathione (GSH) content and sarcous glutathione peroxidase (GSH-Px) activity (p<0.05). Additionally, supplementation with FA increased hepatic GSH-Px activity and decreased sarcous MDA content (p<0.05). However, dietary treatment did not affect the expression of genes related to nuclear factor, erythroid 2-like 2 (NFE2L2) pathway. These results suggest that dietary FA and VE could partially improve meat quality and antioxidant capacity of finishing pigs, but not by activating NFE2L2 pathway under the normal conditions of farming.

Herbicidal Activity of Rotation Crop Residues on Weeds and Selectivity to Crops (윤작작물 잔류물의 제초활성 및 작물에 대한 선택성)

  • Uddin, Md. Romij;Pyon, Jong-Yeong
    • Korean Journal of Agricultural Science
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    • v.37 no.1
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    • pp.1-6
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    • 2010
  • Allelopathic activity of rotation crop residues to common weeds and selectivity of crops were studied for developing an alternative weed control strategy in upland organic farming. All rotation crop residues with various ratios suppressed weed growth, but the growth of crop species stimulated a little when those were grown with the mixture of rotation crop residues except a few crop species. Among the rotation crop residues used in this study hairy vetch was the most effective, followed by Chinese milkvetch, barley and rye in suppression of weed growth. The 90:10 (crop:soil, v/v) treatments incorporation rate of crop residues such as hairy vetch and Chinese milkvetch suppressed completely the growth of all weed species tested in this study. The effect of crop residues on suppression of weed growth reduced to 90% when the incorporation rates decreased to 60% [60:40 (crop:soil, v/v). It was noticed that broadleaf weed species were more susceptible to rotation crop residues than grass weed species. In the influence of crop residues on the crop growth, no growth inhibition was found in red pepper, lettuce and perllia at any incorporation rates, but tomato, cucumber and corn were slightly inhibited in a few cases at their highest incorporation levels. This study demonstrated that rotation crop residues especially hairy vetch and Chinese milkvetch have high herbicidal effects against weeds without inhibiting the growth of crop species.