Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Plant Growth Promotion and Antagonistic Activities Against Anthracnose of Burkholderia sp. LPN-2 Strain

  • Kim, WonChan (School of Applied of Life Science, Kyungpook National University) ;
  • Seo, SangHyun (Research institute, DaeWon Chemical Inc.) ;
  • Lee, ChangHee (School of Applied of Life Science, Kyungpook National University) ;
  • Park, JunHong (Gyeongsangbuk-Do Agricultural Research and Extension Services) ;
  • Kang, SangJae (School of Applied of Life Science, Kyungpook National University)
  • Received : 2016.04.01
  • Accepted : 2016.06.14
  • Published : 2016.06.30
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Abstract

A rhizobacterium LPN-2, which showed strong antifungal activity and auxin producing ability, was isolated from a farmland in North Gyeongsang Province, South Korea. Based on analysis of the 16S rDNA sequence, strain LPN-2 was identified as a novel strain of Burkholderia and was designated as Burkholderia sp. LPN-2. In vitro experiments showed that the isolated stain LPN-2 significantly produced auxin within 48 hr incubation. In order to check for PGPR function we performed in vivo growth promoting test in different crops, including mung bean, pea and cabbage. Application of Burkholderia sp. LPN-2 showed dramatic growth promoting effect on all the tested plants. We also confirmed siderophore and cellulase productions by Burkholderia sp. LPN-2 using CAS blue agar and CMC plate test. Further treatment with LPN-2 and the crude culture broth was effective in suppressing anthracnose in vitro test and also reduced incidence and severity of anthracnose in apple and pepper. Taken together, we conclude that Burkholderia sp. LPN-2 might be used as organic fertilizer for effective crop production in organic farming.

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References

  1. Ahmad, F., I. Ahmad, and M.S. Khan. 2005. Indole acetic acid production by the indigenous isolates of Azotobacter and florescent Pseudomonas in the presence and absence of tryptophan. Turk J. Biol. 29:29-34.
  2. Alexander, D.B. and D.A. Zuberer. 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol. Fertil. Soils. 12:39-45. https://doi.org/10.1007/BF00369386
  3. Brian, C.L., H. Daniel, and M.L. Aaron. 2011. Use of blue agar CAS assay for siderophore detection. J. Microbiol. Biol. Rduc. 12(1):51-53. https://doi.org/10.1128/jmbe.v12i1.249
  4. Coenye, T. and P. Vandamme. 2003. Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol. 5:719-729. https://doi.org/10.1046/j.1462-2920.2003.00471.x
  5. Glick, B.R. 1995. The enhancement of plant growth by free living bacteria. Can. J. Microbiol. 41:109-117. https://doi.org/10.1139/m95-015
  6. Gross, D. and B. Parthier. 1994. Novel natural substances acting in plant growth regulation. J. Plant Growth Regul. 13:94-114.
  7. Han, J., X. Dong, Z. Cai, X. Sun, H. Yang, Y. Wang, and W. Song. 2005. Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph amd a potential biocontrol agent against various plant pathogens. Syst Appli. Microbiol., 28:66-76. https://doi.org/10.1016/j.syapm.2004.09.003
  8. Jung, H.K., J.R. Kim, S.M. Woo, and S.D. Kim. 2006. An auxin producing plant growth promoting rhizobacterium Bacillus subtilis AH18 which has siderophore-producing biocontrol activity. Korean J. Microbiol. Biotechnol. 34:94-100.
  9. Jung, H.K., J.R. Kim, S.M. Woo, and S.D. Kim. 2007. Selection of the auxin, siderophore, and cellulase-producing PGPR, Bacillus licheniformis K11 and its plant growth promoting mechanisms. J. Korean Soc. Appl. Biol. Chem. 50(1):23-28.
  10. Kim, S., K.T. Kim, Y. Chae, A. Jamal, and D.G. Oh. 2008. A survey of genes differently expressed in response to Colletotrichum infection on chili pepper fruit. Kor. J. Hort. Sci. Technol. 26(SUPPL.II):1-8.
  11. Kloepper, J.W. 1993. In Plant growth-promoting rhizobacteria as biological control agents. F. B. J. Metting (ed), Soil Microbial Ecology, Marcel Dekker, New York, NY. pp. 255-274
  12. Kloepper, J.W., A. Gutierrez-Estrada, and J.A. Mclnroy. 2007. Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth promoting rhizobacteria. Can. J. Micriol., 53:159-167. https://doi.org/10.1139/w06-114
  13. Lee, S.E., HS. Yi, S.H. Park, and S.Y. Kim. 2005. Characterization of a rhizobacterium promoting early growth in maize. Korean J. Microbiol. Biotechnol. 33:70-73.
  14. Martinez-Viveros, O., M.A. Jorquera, D.E. Crowley, G. Gajardo, and M.L. Mora. 2010. Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria. J Soil Plant Nutr 10:293-319
  15. Pozom, M.J., C. Azcon-Aguilar, C. Dumas-Gaudot, and J.M. Barea. 1999. 1, 3-g-glucanase activities in tomato roots inoculated with arbuscular mycorrhizal fungi and photophthora parasitica and their possible involvement in bioprotection. Plant Sci. 141:149-157. https://doi.org/10.1016/S0168-9452(98)00243-X
  16. Ryu, C.M., J.W. Kim, O.H. Choi, S.Y. Park, S.H. Park, and C.S. Park. 2005. Nature of a root-associated Paenibacillus polymyxa from field-grown winter barely in Korea. J. Microbiol. Biotechnol. 15:984-991.
  17. Schroth, M.N. and J.G. Hancock. 1982. Disease-suppressive soil and root-colonizing bacteria. Science. 216:1367-1381. https://doi.org/10.1126/science.216.4553.1367
  18. Sopheareth, Mao, S. J. Lee, H. Hwangbo, Y. W. Kim, K. H. Park, G. S. Cha, R.D. Park, and K. Y. Kim. 2006. Isolation and characterization of antifungal substances from Burkholderia sp. culture broth. Curr. Microbiol. 53:358-364. https://doi.org/10.1007/s00284-005-0333-2
  19. Swain M.R., S.K. Naskar, and R.C. Ray. 2007. Indole-3-acetic acid production and effect of sprouting of yam(Dioscorea rotundata L.) minisetts by Bacillus subtilus isolated from culturable cowdung microflora. Pol J Microbiol 56:103-110.
  20. Vandana, K. and R. Goel. 2004. Improved plant growth from seed bacterization using siderophore overproducing cold resistant mutant of Pseudomonas fluorescens. J. Micro. Biotech. 4:653-657.
  21. Vanpinder, S. and B.J. Deverall. 1984. Bacillus subtilis as a control agent against fungal pathogens of citrus fruit. Trans. Br. Mycol. Soc. 83:487-490. https://doi.org/10.1016/S0007-1536(84)80045-5