• Mycobiology
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• 제46권1호
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• pp.72-78
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• 2018

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

• 생물환경조절학회지
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• 제29권4호
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• pp.406-413
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• 2020

멜론(Cucumis melo L.)의 코이어 배지 수경재배 시 고품질 과실을 생산하기 위한 적정 착과 절위, 적심 절위 및 수확 시기를 구명하고자 하였다. 코이어 배지 슬라브(100 × 20 × 10cm)에 3주를 정식하였다. 양액은 야마자키 멜론 표준액을 이용하였고, 급액 농도는 '초기-중기(과실 비대기)-후기'의 생육 단계별로 1.8-2.0-2.3dS·m^-1 공급하였다. 착과 및 적심 절위 실험은 '피엠알달고나'와 '얼스아이비' 2품종을 이용하였다. 착과 절위 실험은 8-10, 11-13 및 14-15 마디에 각각 3처리하였다. 적심 절위 실험은 18, 21 및 24 마디에 각각 3처리하였다. 과실 수확시기 실험은 '피엠알달고나'와 '얼스크라운' 2품종을 이용하여 착과 45일, 50일, 55일 및 60일 후로 4처리하였다. '피엠알달고나' 품종에서 11-13마디 이상 착과 시, 엽폭28.2cm, 엽면적은 10,845㎠로 가장 컸다. 줄기 길이는 '얼스아이비' 품종에서 11-13마디 착과 시 147.6cm로 가장 길었다. 과중은 '얼스아이비' 품종에서 11-13마디 착과시 2.0kg으로 가장 컸다. 과실의 가용성 고형물 함량(SSC)은 '피엠알달고나' 품종에서 8-10 마디 착과시 14.5°Brix, 24 마디 적심시 14.0°Brix로 각각 유의성 있게 가장 높았다. 착과 절위가 낮아질수록 SSC값이 증가하는 경향이 두 품종에서 동일하게 나타났다. '피엠알달고나'와 '얼스크라운' 2품종 모두 착과 55-60일 후 수확된 과실의 SSC 값과 과중이 가장 우수하였다. 종합적으로 검토하면 대부분 SSC값은 착과 절위가 낮아지고 적심 절위가 높아질수록 증가하고, 과중은 착과 절위가 높아질수록 증가하는 경향을 보였다. 착과 후 일수가 증가할수록 과실의 SSC값이 증가하였으며 다양한 품종에 대한 추가 연구가 더 필요하다고 생각되었다. 따라서 코이어 배지를 이용한 수경재배 시 멜론 품종 별로 특성을 잘 파악하여 착과 절위, 적심 절위 및 과실 수확시기를 설정하여야 한다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.47-47
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• 2014

In order to explore mycelial growth and fruiting body formation of Lyophyllum decates on different media, ten cultivation media by using cottonseed hull, sawdust, corn cob etc. as main components were designed for seven strains. The results showed that the mycelial colour of all strains are mainly snow-white, and the formula of media using corn cob as main materials was better than that using cottonseed hull and sawdust for mycelial growth, but no fruiting body was formed. The cottonseed hull medium with a small amount of sawdust, plant leaves, humus or fermented material and wheat was beneficial for fruiting formation. The incubation period for fruiting formation of strain 3001 was 108 days and the highest yield was-214.80 g/bag. Fructification of the strains tasted occurs successively in order of 3001, 1035, 1004 and 1013. It was concluded that different medium composition had significant effect on the mycelial growth and fruiting body formation.

• Mycobiology
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• 제35권4호
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• pp.230-234
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• 2007

In order to breed a Cordyceps bassiana isolate that stably forms fruiting body in artificial cultivation, isolates derived from subculturing and single spores were tested through mating. From C. bassiana EFCC 783, three subcultured isolates EFCC 2830, EFCC 2831 and EFCC 2832 were obtained and fourteen single conidial isolates were obtained from these three subcultured isolates. Two different morphological types were found in the fourteen single conidial isolates. One type was able to form synnemata and another type was not able to form synnemata. Since switch of morphological type was not observed despite their continuous subculturing, cross was performed between the two types and the formation of fruiting body was examined. Ascospores were obtained from a selected fruiting body formed by hybrid of the cross. Self-cross and combinational cross of the ascospore-derived isolates generated hybrids that stably produce high quality fruiting body in artificial media.

• 한국버섯학회지
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• 제12권1호
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• pp.1-7
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• 2014

This study was initiated to investigate antioxidant, anti-acetylcholinesterase, and xanthine oxidase inhibitory activities and properties of fruiting bodies, mycelia, and fermentation culture filtrates from Phellinus igniarius. The contents of total phenols and flavonoid of fruit bodies, mycelia, and culture filtrate were 15.35-1.36 mg/g, 10.35-7.85 mg/g, and 8.25-5.36 mg/g. The 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging abilities of the extracts from the fruiting bodies, mycelia, and culture filtrates were 90.25-95.60%, 78.82-85.24%, and 76.32-82.50% at $50-400{\mu}g/mL$, respectively. The chelating ability of fruiting body extract on ferrous ions was higher than those of mycelia and culture filtrates tested. The anti-acetylcholinesterase inhibitory activity of the fruiting body extract at 400 ${mu}g/mg$ exhibited 91.10% on AChE, which is lower than that of positive control, galanthamine (94.82%). The xanthine oxidase inhibitory activities of the fruiting bodies, mycelia, and culture extract were 85.47%, 78.13%, and 72.49% at 400 ${\mu}g/mL$, respectively. Overall, the fruiting body extract has better anti-acetylcholinesterase, antioxidant and xanthine oxidase inhibitory activities than those from mycelia and culture filtrate.

• Mycobiology
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• 제37권3호
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• pp.230-237
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• 2009

The oyster mushroom (Pleurotus ostreatus) is one of the most important edible mushrooms worldwide. The mechanism of P. ostreatus fruiting body development has been of interest both for the basic understanding of the phenotypic change of the mycelium-fruiting body and to improve breeding of the mushrooms. Based on our previous publication of P. ostreatus expressed sequence tag database, 1,528 unigene clones were used in macroarray analysis of mycelium, fruiting body and basidiospore developmental stages of P. ostreatus. Gene expression profile databases generated by evaluating expression levels showed that 33, 10, and 94 genes were abundantly expressed in mycelium, fruiting body and basidiospore developmental stages, respectively. Among them, the genes specifically expressed in the fruiting body stage were further analyzed by reverse transcription-polymerase chain reaction and Northern blot to investigate temporal and spatial expression patterns. These results provide useful information for future studies of edible mushroom development.

• Mycobiology
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• 제37권2호
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• pp.109-113
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• 2009

The mineral contents of the cultivation substrates, fruiting bodies of the mushrooms, and the postharvest cultivation substrates were determined in cultivated edible mushrooms Pleurotus eryngii, Flammulina velutipes, and Hypsizigus marmoreus. The major mineral elements both in the cultivation substrates and in the fruiting bodies were K, Mg, Ca, and Na. Potassium was particularly abundant ranging 10${\sim}$13 g/kg in the cultivation substrates and 26${\sim}$30 g/kg in the fruiting bodies. On the contrary, the calcium content in the fruiting bodies was very low despite high concentrations in the cultivation substrates, indicating Ca in the cultivation substrates is in a less bio-available form or the mushrooms do not have efficient Ca uptake channels. Among the minor mineral elements determined in this experiment, Cu, Zn, and Ni showed high percentage of transfer from the cultivation substrates to the fruiting bodies. It is noteworthy that the mineral contents in the postharvest cultivation substrates were not changed significantly which implies that the spent cultivation substrates are nutritionally intact in terms of mineral contents and thus can be recycled as mineral sources and animal feeds.

• Mycobiology
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• 제49권2호
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• pp.161-172
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• 2021

Phellinus strains were collected from different areas in Korea. Of them, the fast mycelial growing strains were artificially cultivated on the oak logs to produce fruiting body. The varieties, Phellinus linteus ASI26099 (Korea Sanghwang) and P. baumii PBJS (Jangsoo Sanghwang) were grown under the same conditions as controls. Their cultivating characteristics including mycelial colonization, pinhead formation, and fruiting body formation rate were investigated on the logs. Basidiocarps of Phellinus strains HN00K9, HN6036, and ASI26099 were concentrically zonate and shallowly sulcate, and dark chestnut showing typical characteristics of Tropicoporus linteus (synonyum: P. linteus, Inonotus linteus, polyporus linteus), which is distinguishably different to PBJS. HN00K9 showed the highest yield of fruiting body among the mushroom strains. The β-glucan content in fruiting bodies of HN00K9 was 20% higher than those of other strains. Bioactive effects of polysaccharide samples from fruiting bodies of Phellinus strains, HN00K9, HN6036, ASI26099, and PBJS were assessed on cell viability and cytokine (IL-6 and TNF-α) inhibition and finally on anticancer to different human cancer cells.

• 한국산림과학회지
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• 제100권3호
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• pp.432-440
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• 2011

밤나무의 수고와 근원경은 액비처리구와 화학비료처리구에서 가장 많은 생장량을 나타냈으며 수관폭 생장량은 동-서방향과 남-북방향에서 각각 액비처리구와 화학비료처리구에서 우수하였다. 결과지의 총 길이와 착구부~정단부의 길이생장은 액비처리구에서 가장 우수하였으며 기부~착구부의 길이는 액비처리구와 화학비료처리구에서 동일하게 가장 길게 생장하였다. 결과지의 기부직경과 착구부의 상하부직경은 모두 액비처리구에서 가장 두껍게 생장하였다. 본 연구의 5개의 생장지수(결과지발생지수, 길이생장지수, 착구부상하직경비율, 직경생장지수, 굵기지수)에서 길이생장지수만이 액비처리구에서 가장 높고 나머지 모든 지수는 화학비료처리구에서 가장 높았다. 결과모지당 가지발생수와 소약지 개수는 무처리구와 액비처리구에서 많았으며 처리간 유사한 경향을 보였다. 결과모지당 결과지수는 화학비료처리구와 유기질비료처리구에서 많았으며, 결과지당 착구수는 유기질비료처리구와 액비처리구에서 많았다. 과실의 중량과 수량을 기준으로 한 상품화 비율은 무처리구(87.5%)와 화학비료처리구(84.6%)에서 높았으나 액비처리구의 상품화 비율은 각각 84.3%와 82.7%로 평균 수준이었다. 평균입중과 총착구수는 각각 액비처리구와 화학비료처리구로 가장 우수하였다. 함과수는 처리 간 차이는 없었으며 본당수확량은 화학비료처리구(8.2 kg)와 액비처리구(8.0 kg)에서 다수확성을 보였으나 처리간 통계적으로 유의한 차이는 없었다. 과실의 중량과 수량을 기준으로 등급을 구분한 결과, 액비처리구에서 대립 이상의 비율이 각각 43.5%와 34.3%로 가장 높아 액비처리에서 고부가가치의 고품질 밤이 가장 많이 생산되었다.

• 한국균학회지
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• 제30권1호
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• pp.6-10
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• 2002

배양병의 선발시험에서는 1100 cc pp병에서 자실체 형성이 우수하였다. 경제성을 고려하여 1000 cc병을 이용한 자실체형성에서 현미의 입병량은 $60{\sim}80\;g$이 적당하였다. 자실체 형성을 위한 배양온도는 $24^{\circ}C$에서 균사 배양한 후 $20^{\circ}C$에서 발아를 유도하였을 때 자실체 형성이 우수하였다. 현미배지의 sucrose첨가량은 10%를 첨가하고 번데기첨가량은 병당 10 g을 첨가하였을 때 자실체 형성이 양호하였으며 peptone과 hemoglobin, 건조누에 등은 자실체의 수확량을 증가시키지 못하였다. 현미배지의 공극률에서는 $0.59{\sim}0.63%$의 공극률에서 자실체 형성이 양호하였다. 광조사의 시간은 500 lx, 12시간 정도가 적당하였다.