Mycobiology

The Korean Society of Mycology (한국균학회)
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Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

  • Yao, Fang-Jie (College of Horticulture, Jilin Agricultural University) ;
  • Lu, Li-Xin (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University) ;
  • Wang, Peng (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University) ;
  • Fang, Ming (College of Horticulture, Jilin Agricultural University) ;
  • Zhang, You-Min (College of Horticulture, Jilin Agricultural University) ;
  • Chen, Ying (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University) ;
  • Zhang, Wei-Tong (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University) ;
  • Kong, Xiang-Hui (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University) ;
  • Lu, Jia (College of Horticulture, Jilin Agricultural University) ;
  • Honda, Yoichi (Graduate School of Agriculture, Kyoto University)
  • Received : 2017.06.17
  • Accepted : 2018.01.19
  • Published : 2018.03.31
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Abstract

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

Keywords

References

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