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Antioxidant and Anti-inflammatory activity of Silvetia siliquosa extract (Silvetia siliquosa 추출물의 항산화 및 항염효과)

  • Kim, Kyoung-Sook;Kim, Sook-hee
    • Journal of Convergence for Information Technology
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    • v.11 no.8
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    • pp.232-239
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    • 2021
  • In this study, the antioxidant and anti-inflammatory properties of Silvetia siliquosa extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 54.85 ± 2.79 mg/g was shown. Flavonoids showed 18.70 ± 5.26 mg/g. The DPPH experiment showed an antioxidant function of 3.950 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 7.418 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 6.056 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 3.633 ± 0.280 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Silvetia siliquosa extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 26.94±0.52% at a concentration of 100 ㎍/mL. These results indicate that Silvetia siliquosa extract is available as an anti-inflammatory cosmetic material.

Antioxidant and Anti-inflammatory activity of Sargassum patens extract (쌍발이모자반(Sargassum patens) 추출물의 항산화 및 항염효과)

  • Kim, Sook-hee
    • Journal of Convergence for Information Technology
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    • v.11 no.7
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    • pp.264-271
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    • 2021
  • In this study, the antioxidant and anti-inflammatory properties of Sargassum patens extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 18.99±0.69 mg/g was shown. Flavonoids showed 11.89±1.16 mg/g. The DPPH experiment showed an antioxidant function of 19.78 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 63.64 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 7.966 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 2.089 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Sargassum patens extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 30.64±0.23% at a concentration of 100 ㎍/mL. These results indicate that Sargassum patens extract is available as an anti-inflammatory cosmetic material.

Antioxidant and Anti-inflammatory activity of Colpomenia sinuosa extract (불레기말 추출물의 항산화 및 항염효과)

  • Cho, Young Jae;Kim, Sook-hee;Choi, Jae Young;Lee, Ja-bok
    • Journal of Convergence for Information Technology
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    • v.12 no.1
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    • pp.135-143
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    • 2022
  • In this study, the antioxidant and anti-inflammatory properties of Colpomenia sinuosa extracts were identified. Antioxidant experiments included DPPH, ABTS, nitrite scavenging experiments, and FRAP, polyphenol concentration measurements, flavonoid concentration measurements. DPPH assay results showed an antioxidant function of 2.821 mg ascorbic acid/g extract. ABTS assay results showed an antioxidant function of 3.923 mg ascorbic acid/g extract. nitrite assay results showed an antioxidant function of 17.89 mg ascorbic acid/g extract. In FRAP, 1 mg of the Colpomenia sinuosa extract showed a reduction of 2.062±0.177 ㎍ of ascorbic acid. For polyphenols, 62.85±3.18 mg/g was shown. Flavonoids showed 10.01±0.24 mg/g. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions. In cytotoxicity experiments, Colpomenia sinuosa extracts showed cytotoxicity of less than 20% and an inflammatory inhibition of 30.93±2.93% at a concentration of 100 ㎍/mL. These results indicate that Colpomenia sinuosa extract is available as functional cosmetic material.

The Antioxidant Capacity and Whitening Effects of the Extract from the Mixture of 7 Different Vegan Cosmetic Ingredients (비건(Vegan)인증 화장품 원료 7종 혼합추출물의 항산화능 및 미백효과 연구)

  • Choi, In-Jeong
    • Journal of the Korean Applied Science and Technology
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    • v.39 no.1
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    • pp.10-17
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    • 2022
  • This study attempted to investigate the antioxidant capacity and whitening effects of the extract from the mixture of 7 different vegan cosmetic ingredients. To examine radical scavenging capacity, DPPH, ABTS and FRAP assays were performed. In addition, polyphenol and flavonoid concentrations were measured to check antioxidant substances. Specifically, in a DPPH test, 105.74 mg ascorbic acid/g antioxidant capacity was observed. In an ABTS assay, 85.31 mg ascorbic acid/g antioxidant capacity was found. Lastly, in an FRAP assay, 1mg of the extract revealed ascorbic acid 198.01±5.50 ㎍ reducing power. Polyphenol and flavonoid concentrations were 30.19±0.75 mg/g and 9.12±0.36 mg/g respectively. In a cell-based assay, cytotoxicity and whitening activity were examined. In terms of cytotoxicity, '20% or less' was observed. Furthermore, the inhibition of melanin synthesis was '34.70±2.97%' at 100 ㎍/mL, confirming the possibility of the extract from the mixture of 7 different substances as a cosmetic ingredient.

Evaluation of a Dietary Organic Selenium Supplement at Different Dietary Protein Concentrations on Growth Performance, Body Composition and Antioxidative Status of Broilers Reared under Heat Stress

  • Khajali, Fariborz;Raei, Ali;Aghaei, Ali;Qujeq, Dordi
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.4
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    • pp.501-507
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    • 2010
  • Three hundred chicks were randomly assigned among four treatments to evaluate the effect of an organic selenium supplement at various levels of dietary protein. Two levels of supplemental selenium (0 and 0.3 mg/kg) from zinc-L-selenomethionine were tested at two levels of dietary protein (normal and reduced) in a completely randomized design with $2{\times}2$ factorial layout. The experiment lasted up to 49 d of age during which all birds were exposed to $31{\pm}1^{\circ}C$. The effects of selenium or its interaction with CP on growth performance and carcass characteristics were not significant. However, feeding the reduced-CP diet decreased weight gain in the starting period and increased liver and abdominal fat weights relative to body weight. Ferric reducing ability of plasma (FRAP) was not significantly affected by dietary CP and Se or their interaction though FRAP values were numerically higher in the Se-supplemented group. Dietary CP content did not affect the activity of plasma glutathione peroxidase (GSHPx), though Se significantly elevated plasma GSHPx activity. The interaction of CP and Se was not significant for FRAP and plasma GSHPx activity.

Antioxidant Capacity and Associated Factors during the Chronic Phase after Stroke (만성 뇌졸중 환자의 항산화능에 영향을 주는 요인에 관한 연구)

  • Choi, Seung-Hye;Kwak, Chung-Shil;Choi-Kwon, Smi
    • Journal of Korean Biological Nursing Science
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    • v.16 no.1
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    • pp.52-59
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    • 2014
  • Purpose: The purpose of this study was to investigate antioxidant capacity and relating factors including gender, obesity, lifestyle factors, and nutrient intake in chronic stroke patients. Methods: A total of 188 chronic stroke patients who visited a medical center in Seoul participated in the study. A structured questionnaire was used to assess their clinical characteristics and lifestyles. Blood samples were collected for ferric reducing ability of plasma (FRAP) for antioxidant capacity. Dietary intake of the patients were obtained for 2 days by 24 hours recall method. Results: The mean FRAP was $392.0{\mu}mol/L$. According to the hierarchical multiple regression analysis, the factors related to high FRAP were male sex (p=.036), alcohol drinking (p=.013), and calorie intake deficiency (p=.005). Conclusion: We found that antioxidant capacity was related to lifestyle factors including alcohol drinking, and calorie intake in chronic stroke patients. A tailored strategy is needed to increase antioxidant capacity according to gender and lifestyles in the chronic phase of stoke patients.

Effect of Addition of Mushroom and Sea Tangle Extracts and Mustard Leaf on Anti-oxidant Properties of Kimchi (버섯 및 다시마 추출물과 갓의 첨가가 김치의 항산화 특성에 미치는 영향)

  • Ha, Seon-Hye;Kang, Soon Ah
    • The Korean Journal of Food And Nutrition
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    • v.31 no.4
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    • pp.471-477
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    • 2018
  • The antioxidant properties of mushroom and sea tangle extracts and mustard leaf added to Kimchi were investigated by total phenolic content, DPPH radical scavenging, ABTS anion scavenging, FRAP reducing power, and ORAC value. Total phenolic content of functional Kimchi (FK) was significantly higher than that of control Kimchi (CK). DPPH radical and ABTS anion scavenging activities of functional Kimchi were significantly higher than those of control Kimchi by 2.7 and 1.7 fold, respectively (p<0.05). Also, the FRAP reducing power and ORAC value of functional Kimchi increased compared to those of the control Kimchi by 1.6 and 1.1 fold, respectively (p<0.05). Our results suggest that functional Kimchi made by mushroom and sea tangle extracts and mustard leaf may be a potent antioxidant source and could be developed as a antioxidant functional food that may be for the effective treatment of oxidant conditions. Through continuous research and development of functional Kimchi by the use of mushroom and sea tangle extracts, it will be necessary to try to identify other functions that could be useful for preventing various diseases.

Antioxidant Activity, Total Phenolic Content, Total Flavonoid Content, and Total Anthocyanin Content of Vaccinium oldhamii Miq. Collected from 11 Regions of South Korea

  • Da Hyun Lee;Jun Hyeok Kim;Chung Youl Park;Kyeong Min Kim;Hyeon Min Kim;Un Seup Shin;Do Hyun Kim;Chae Sun Na;Wan Geun Park
    • Korean Journal of Plant Resources
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    • v.37 no.3
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    • pp.235-246
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    • 2024
  • We studied antioxidant activities of Vaccinium oldhamii Miq. from 11 regions in South Korea and blueberries, domestically produced and imported. Correlation analysis between V. oldhamii habitats, environmental factors, and antioxidant properties was conducted. DPPH RC50 values ranged from 220.44 to 902.38㎍/mL, ABTS from 524.29 to 1230.97 ㎍/mL, and FRAP from 1783.71 to 2235.78 ㎛ Fe (II)/g. V. oldhamii from Gumi showed highest DPPH activity, Taean and Haenam for ABTS, and Gimcheon for FRAP. V. oldhamii exhibited superior antioxidant activities compared to blueberries. Meteorological conditions correlated positively with ABTS and DPPH activities, negatively with wind speed and humidity affecting DPPH and phenolic, flavonoid, and anthocyanin contents. Based on these findings, it is suggested that V. oldhamii fruits collected from Gimcheon and Gumi regions can be effectively utilized as natural antioxidants derived from plant materials.

Effect of Artemisia capillaris Extracts on Antioxidant Activity and Allergic Dermatitis (인진호(Artemisia capillaris) 추출물의 항산화 활성 및 알러지성 피부염에 대한 효과)

  • Kim, Jong-Myeung;Shin, Yong-Kyu;Kim, Byung-Oh;Kim, Jong-Kuk;Lee, Sang-Han;Kim, Young-Sup
    • Journal of Life Science
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    • v.22 no.7
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    • pp.958-963
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    • 2012
  • The antioxidant activities of 6 solvent extracts of Artemisia capillaris were evaluated in a dintroflurobenzen (DNFB)-induced allergic mouse model. In vitro antioxidant activities were determined using DPPH and the FRAP test. Methanol (DPPH: 85.87%, FRAP: 1.772) and $dH_2O$ (DPPH: 60.69%, FRAP: 3.185) extracts showed the highest antioxidant activities compared with other solvents (ethyl acetate 41.81%, 0.407, hexane 8.37%, 0.328, etc.). In addition, we tested atopic dermatitis (AD)-like skin lesions in mice treated with DNFB. The methanol extract of A. capillaris on the AD-like skin lesions in DNFB-induced atopy inhibited ear thickness increases (47%) and the skin lesions (45%) compared with a positive control (methanol). The results suggest that they have potential as natural antioxidants and allergy-improving substances and that they may be valuable materials in the functional food or cosmeceutical industry.

Antioxidant Activities of the Extract Fractions from Suaeda japonica (칠면초 분획물의 항산화 활성)

  • Choi, Jong-Il;Kim, Yeon-Joo;Kim, Jae-Hun;Song, Beom-Sok;Yoon, Yo-Han;Byun, Myung-Woo;Kwon, Joong-Ho;Chun, Soon-Sil;Lee, Ju-Woon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.2
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    • pp.131-135
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    • 2009
  • This study was designed to investigate the antioxidant effect of Suaeda japonica grown in Suncheon Bay. S. japonica was extracted using different solvents and the extracts were examined for their antioxidative activities with various methods. When the total phenolic contents were determined, the contents in the ethyl acetate, butanol and methanol extracts were 21.33, 17.31, and 2.33 mg/g, respectively. Fractions of butanol extract recorded the highest values of DPPH radical scavenging activity, $\beta$-carotene bleaching assay, and FRAP assay. The DPPH radical scavenging activities of butanol, ethyl acetate, methanol and water fractions were 77.46, 74.43, 47.99, and 27.70%, respectively. The FRAP value of butanol extract was 2.42 mM. But, the fraction of ethyl acetate extract was recorded the highest TBARS value. These results suggest that S. japonica, the specialty of Suncheon, could be a potential source of natural antioxidants.