Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
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Effect of Artemisia capillaris Extracts on Antioxidant Activity and Allergic Dermatitis

인진호(Artemisia capillaris) 추출물의 항산화 활성 및 알러지성 피부염에 대한 효과

  • Kim, Jong-Myeung (Department of Applied Biology, Kyungpook National University) ;
  • Shin, Yong-Kyu (Research Institute of Biotechnology, Bion Co., Ltd.) ;
  • Kim, Byung-Oh (Department of Applied Biology, Kyungpook National University) ;
  • Kim, Jong-Kuk (Department of Life Science, Kyungpook National University) ;
  • Lee, Sang-Han (Food & Bio-Industry Research Institute, and Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Kim, Young-Sup (Department of Applied Biology, Kyungpook National University)
  • 김종명 (경북대학교 생태환경대학 생태환경관광학부 생물응용전공) ;
  • 신용규 ((주) 바이온 부설 연구소) ;
  • 김병오 (경북대학교 생태환경대학 생태환경관광학부 생물응용전공) ;
  • 김종국 (경북대학교 자연과학대학 생명과학부) ;
  • 이상한 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 김영섭 (경북대학교 생태환경대학 생태환경관광학부 생물응용전공)
  • Received : 2012.04.17
  • Accepted : 2012.07.19
  • Published : 2012.07.30
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Abstract

The antioxidant activities of 6 solvent extracts of Artemisia capillaris were evaluated in a dintroflurobenzen (DNFB)-induced allergic mouse model. In vitro antioxidant activities were determined using DPPH and the FRAP test. Methanol (DPPH: 85.87%, FRAP: 1.772) and $dH_2O$ (DPPH: 60.69%, FRAP: 3.185) extracts showed the highest antioxidant activities compared with other solvents (ethyl acetate 41.81%, 0.407, hexane 8.37%, 0.328, etc.). In addition, we tested atopic dermatitis (AD)-like skin lesions in mice treated with DNFB. The methanol extract of A. capillaris on the AD-like skin lesions in DNFB-induced atopy inhibited ear thickness increases (47%) and the skin lesions (45%) compared with a positive control (methanol). The results suggest that they have potential as natural antioxidants and allergy-improving substances and that they may be valuable materials in the functional food or cosmeceutical industry.

본 연구는 항산화 및 알러지성 피부질환에 대한 효과가 있는 안전성이 확보된 새로운 천연 향장 소재 개발을 위하여 인진호(A. capillaris)를 6종의 용매($dH_2O$, ethanol, methanol, acetone, ethyl acetate and hexane)로 추출한 추출물의 항산화 및 항아토피 활성을 검증하였다. 항산화 활성 검증에서는 methanol (DPPH: 85.87%, FRAP: 1.772)과 $dH_2O$ (DPPH: 60.69%, FRAP: 3.185) 등의 극성 용매 추출물에서 우수한 항산화 활성을 나타내었으나 비극성 용매에서는 활성이 거의 없었다(ethyl acetate 41.81%, 0.407, Hexane 8.37%, 0.328). DNFB-induced atopic dermatitis mouse model에 의한 항 아토피에 대한 치유력 측정에서는 인진호 추출물 처리군은 염증 세포의 수에서 음성 대조군과 비교하여 220% 증가로 양성 대조군의 400% 보다 약 45%의 완화 효과를 보여 주었으며, 귀의 두께는 음성 대조군의 175%로 양성 대조군의 330%보다 약 47%가 감소하는 뚜렷한 증상 완화 효과를 나타내었다. 이러한 결과는 인진호 추출물의 항산화 물질이 극성 물질이며, DNFB 처치에 의한 귀의 비후반응 감소와 염증부위의 면역세포 침투현상을 감소시키는 염증반응 완화 기능성 물질을 포함하고 있을 가능성을 알 수 있었다. 이상의 결과를 바탕으로 인진호 추출물에 대한 물리적 특성, 유전자 발현과 면역학적 실험 등이 추가 된다면 새로운 향장소재나 기능성 식품의 소재로도 이용 가능할 것으로 판단된다.

Keywords

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