• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.21 no.5
• /
• pp.515-524
• /
• 2008

The ultimate behavior of high-tension bolted joints with various plate thickness and bolt size is investigated using nonlinear F.E. analysis and experimental study. The relation with sliding load, bolt deformation, and failure modes are presented based on plate thickness and bolt size. Three kinds of the bolt diameter(M20, M22, M24) and five types of the steel plates (l2mm, 16mm, 20mm, 30mm, 40mm) are considered for the ultimate behavior of the bolted joints. The numerical model, constructed by commercial F.E. program, ABAQUS, of ultimate behavior of bolted joints is introduced and verified by experimental results. The force-displacement and force-axial strain relations are measured and compared with the results by 3D finite element analysis.

• Food Science and Biotechnology
• /
• v.15 no.5
• /
• pp.694-697
• /
• 2006

The initiation of immunoglobulin E (IgE)-mediated allergic reactions requires binding of IgE antibody to its high-affinity receptor. Human basophilic KU812F cells express $Fc{\varepsilon}RI$ on the cell surface and act as effector cells in the allergic response. In this study, we investigated the effects of Scutellaria radix extract on the expression of the $Fc{\varepsilon}RI$ in human KU812F cells. Flow cytometric analysis showed that S. radix extract treatment caused a concentration-dependent decrease in $Fc{\varepsilon}RI$ expression on the cell surface. Furthermore, the level of $Fc{\varepsilon}RI$ ${\alpha}$, ${\beta}$, and ${\gamma}$ chain mRNA in KU812F cells was examined by RT-PCR. S. radix extract reduced total cellular $Fc{\varepsilon}RI$ $\alpha$ and ${\gamma}$ chain mRNA expression in a concentration-dependent manner. $Fc{\varepsilon}RI$-mediated histamine release was reduced from $21.75{\pm}1.34\;ng/10^6$ cells in non-treated cells to $16.46{\pm}1.98\;ng/10^6$ cells in S. radix extract treated cells. These results suggested that S. radix extract has the potential to down-regulate of FcRI expression and to inactivate basophils.

• Animal Bioscience
• /
• v.35 no.7
• /
• pp.1010-1020
• /
• 2022

Objective: The experiment was conducted to evaluate the effects of maternal undernutrition during late pregnancy on the expressions of genes involved in growth and development in ovine fetal perirenal brown adipose tissue (BAT). Methods: Eighteen ewes with singleton fetuses were allocated to three groups at day 90 of pregnancy: restricted group 1 (RG1, 0.33 MJ metabolisable energy [ME]/kg body weight [BW]^0.75/d, n = 6), restricted group 2 (RG2, 0.18 MJ ME/kg BW^0.75/d, n = 6), and a control group (CG, ad libitum, 0.67 MJ ME/kg BW^0.75/d, n = 6). The fetuses were removed at day 140 of pregnancy. All data were analyzed by using the analysis of variance procedure. Results: The perirenal fat weight (p = 0.0077) and perirenal fat growth rate (p = 0.0074) were reduced in RG2 compared to CG. In fetal perirenal BAT, the protein level of uncoupling protein 1 (UCP1) (p = 0.0001) was lower in RG1 and RG2 compared with CG and UCP1 mRNA expression (p = 0.0265) was decreased in RG2. The protein level of myogenic factor 5 (Myf5) was also decreased in RG2 (p = 0.0001). In addition, mRNA expressions of CyclinA (p = 0.0109), CyclinB (p = 0.0019), CyclinD (p = 0.0015), cyclin-dependent kinase 1 (CDK1) (p = 0.0001), E2F transcription factor 1 (E2F1) (p = 0.0323), E2F4 (p = 0.0101), and E2F5 (p = 0.0018) were lower in RG1 and RG2. There were decreased protein expression of peroxisome proliferator-activated receptor-γ (PPARγ) (p = 0.0043) and mRNA expression of CCAAT/enhancer-binding protein-α (C/EBPα) (p = 0.0307) in RG2 and decreased PPARγ mRNA expression (p = 0.0008) and C/EBPα protein expression (p = 0.0015) in both RG2 and RG1. Furthermore, mRNA expression of bone morphogenetic protein 4 (BMP4) (p = 0.0083) and BMP7 (p = 0.0330) decreased in RG2 and peroxisome proliferator-activated receptor co-activator-1α (PGC-1α) reduced in RG2 and RG1. Conclusion: Our observations support that repression of regulatory factors promoting differentiation and development results in the inhibition of BAT maturation in fetal perirenal fat during late pregnancy with maternal undernutrition.

• Proceedings of the KIEE Conference
• /
• 2009.07a
• /
• pp.704_705
• /
• 2009

This paper presents a axial flux permanent magnet synchronous generator(AFPMSG), which is suitable for both vertical-axis and horizontal-axis wind turbine generation system. The design and construction features of the AFPMSG are reviewed. The characteristic analysis is performed such as cogging torque and e.m.f waveform, with the aid of a 3D finite element method. The experimental results confirm the characteristic analysis developed.

• Architectural research
• /
• v.3 no.1
• /
• pp.45-52
• /
• 2001

In this paper, a pressurized single vertical arch and a pressurized leaning arch composed of flexible fabric material are considered. These arches have also been considered as a possible support structure for the tent-like structures. Two different boundary conditions are considered in leaning arches with fixed bases and pinned bases. The behaviors of the leaning arches are investigated for two tilt angles as 15, 30. For each angle, two loading conditions are considered as uniformly distributed load and wind loads. The F.E.M. is used through the all analysis procedures. For the results, load-deflection relationships, buckling modes, differences between two boundary conditions and deformed configurations are discussed.

• Applied Chemistry for Engineering
• /
• v.35 no.4
• /
• pp.335-340
• /
• 2024

In this paper, we synthesized organic and inorganic hybrid materials to introduce antibody functionality to UIO-66 and incorporated them into a surface plasmon resonance (SPR) assay to enhance the sensitivity of detecting small molecules such as oxytocin. A biological marker peptide called oxytocin may help in the diagnosis of heart failure, Alzheimer's disease, and cancer. To detect oxytocin at concentrations as low as a few femtomole (fM), we developed a surface sandwich assay utilizing a pair of oxytocin-specific antibodies for enhancing selectivity and one of metal organic frameworks [e.g., UiO-66-(COOH)₂] possessing high porosity and surface-area as a signal amplifier. Initially, real-time SPR assays were used to confirm that each selected oxytocin-specific antibody binds strongly to oxytocin and to different binding sites on oxytocin. One of these antibodies (e.g., anti-OXT[OTI5G4]) was immobilized on the surface of a thin gold chip. Upon sequential injecting of oxytocin and the other antibody (e.g., anti-OXT[4G11]) conjugated to UiO-66-(COOH)₂ onto the surface to form the surface sandwich complex of anti-OXT[OTI5G4]/oxytocin/UiO-66-(COOH)₂-anti-OXT[4G11]), SPR changes, which varied with oxytocin concentration, were then measured in real time. The results demonstrated that sensitivity was amplified by over a million-fold compared to assays without UiO-66-(COOH)₂, enabling oxytocin detection down to approximately 10 fM.

• Korean Chemical Engineering Research
• /
• v.53 no.6
• /
• pp.723-729
• /
• 2015

The moment analysis of cyclic adenosine monophosphate (cAMP) was performed using chromatograms that were obtained with the pulse input method from an octadecyl silica (ODS) high-performance liquid chromatography (HPLC) column. The general rate (GR) model was employed to calculate the first absolute moment and the second central moment. Three important coefficients for moment analysis, which are molecular diffusivity ($D_m$), external mass transfer coefficient ($k_f$), and intra-particle diffusivity ($D_e$), were estimated by the Wilke-Chang equation, Wilson-Geankoplis equation, and comparing van Deemter equation to theoretical plate number equation, respectively. Experiments were conducted by various conditions of flow rates, methanol volume ratio of the mobile phase, and solute concentration. After the moment analysis, results were organized by van Deemter plots. Also van Deemter coefficients were compared each other to effect $H_{ax}$, $H_f$, and $H_d$ on height equivalent to a theoretical plate (HETP, $H_{total}$). The value of intraparticle diffusion ($H_d$) was the primary factor which makes for HETP whereas external mass transfer ($H_f$) was disregardable factor.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.24 no.9 s.180
• /
• pp.2266-2273
• /
• 2000

Equations of motion of a multi-beam system undergoing overall rigid body motion are derived by employing finite element method. An orientation angle is employed to allow the arbitrary orientation o f the beam element. Modal coordinate reduction technique, which has been successfully utilized in the conventional linear modeling method, is employed for the present modeling method to reduce the computational effort. Different from the conventional linear modeling method, the present modeling method captures the motion-induced stiffness variations which are important for the dynamic analysis of structures undergoing overall rigid body motion. The numerical results are compared to those of a commercial program to verify the reliability of the present method.

• Proceedings of the KIEE Conference
• /
• 2000.11b
• /
• pp.241-243
• /
• 2000

In this paper, we analyze three-dimensional magnetic field distribution of a convergence purity maget(C.P.M) which is used for a cathode ray tube. The magnetization vector distribution of the C.P.M is obtained from the result of magnetization process analysis using the 2D F.E.M. Then the motion of electron beam passing through the inner space of the C.P.M is determined and compared with experimental result.

• Korean Journal of Environment and Ecology
• /
• v.35 no.6
• /
• pp.632-643
• /
• 2021

The objective of this study was to examine vegetation community structure and distribution of Fraxinus chiisanensis in Mt.Minjuji of Chungcheongbuk-do by setting up and surveying 8 plots (400 m² each). Mean Importance Value (MIV) of Fraxinus chiisanensis in 8 plots was 35.19% in average (ranging from 26.07~42.74%). Since it is the dominant species in all plots, it is expected to maintain the present vegetation structure. The analysis of the DBH (diameter at breast height) showed that the diameter of Fraxinus chiisanensis in Mt.Minjuji ranges from 2 to 43cm. The majority of Fraxinus chiisanensis is expected to maintain current state unless disturbance or rapid environmental change occurs. The Species Diversity (H') was 0.8498~1.0261, Evenness (J') was 0.8160~0.9256, Dominance Index (D) was 0.0789~0.1840, Maximum Diversity (H'max) was 1.0414~1.2041. The analysis of annual ring and radial growth showed that the average age of Fraxinus chiisanensis in Mt.Minjuji was 29.1years(ranging from 22~58years). The average annual radial growth of Fraxinus chiisanensis was the highest in community G with 5.84mm and the lowest in community B with 2.80mm. The similarity index analysis revealed that the similarity index between community B and E, C and F, H was the highest with 69.0%, and the similarity index between community E and F was the lowest with 29.6%. Both the area of Fraxinus chiisanensis community of Mt.Minjuji and its population size are very small. Therefore, this area needs to be designated as Forest Genetic Resource Reserve.