• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.304-308
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• 2021

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

• 식물분류학회지
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• 제39권1호
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• pp.48-54
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• 2009

우리나라 특산식물이며 희귀식물인 만리화(물푸레나무과) 집단의 유전적 다양성을 조사하기 위하여 5 집단 84개체에 대한 ISSR (Inter-Simple Sequence Repeats) 표지자 분석을 실시하였다. 14개의 ISSR 프라이머에서 총 93개의 증폭산물을 관찰할 수 있었으며, 유전적 다양성을 나타내는 P (Percentage of polymorphic loci)값과 S.I. (Shannon's information Index)가 다른 관목류에 비해 비교적 낮게 나타났다. 집단별 유전적 다양성은 석병산집단 (P = 64.5%, S.I. = 0.281)과 설악산B집단(P = 62.4%, S.I. = 0.292)이 높았으며, 석개재집단(P = 37.6%, S.I. = 0.178)이 가장 낮았다. 전체 유전변이 중 30.6%가 집단간에 기인하는 것으로 나타났고, 나머지 69.4%는 집단내 개체간의 차이에서 기인하였다. 이러한 결과는 분포역이 매우 제한되어 있으며 불연속적으로 출연하는 희귀종이라는 점으로 미루어 볼 때 지역간의 유전자 교류가 원활하지 못해 나타난 결과라고 추정해 볼 수 있다. 유전적 거리를 이용하여 UPGMA 방법에 의한 유집분석을 실시한 결과, 집단의 지리적 격리정도와 유전적 연관성은 비교적 일치하는 경향이었다. 본 연구 결과, 만리화의 유전자원보존을 위해서는 자생지 보호와 더불어 동적인 현지외 보존(dynamic ex situ conservation)과 같은 보다 적극적인 대책이 요구되며, 더 높은 유전적 다양성을 확보하기 위해서는 소수의 집단에서 다수 개체를 선발하기보다는 집단당 소수 개체를 다수의 집단에서 선발하는 집단 위주의 보존이 더욱 효과적일 것으로 판단된다.

• 한국자원식물학회지
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• 제26권5호
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• pp.619-627
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• 2013

꼬리말발도리(Deutzia paniculata Nakai)는 전 세계적으로 우리나라 경상남북도 일부지역에만 자생하는 매우 제한된 분포범위를 가지는 특산식물이다. 이러한 꼬리말발도리 집단의 유전적 다양성 및 구조를 조사하기 위해 5집단 155개체에 대한 ISSR(Inter Simple Sequence Repeat) 분석이 수행되었다. 총 6개의 ISSR 프라이머를 이용하여 31개의 증폭산물을 관찰하였으며, 집단 수준에서의 유전적 다양성의 평균은 SI(Shannon's information index)=0.429, h (Nei's genetic diversity)=0.271로, 매우 높은 수준으로 나타났다. 집단별로는 큰 차이는 없었지만 비교적 높은 개화율을 보이는 밀양, 양산 집단이 다른 집단에 비해 다소 높은 유전 다양성을 유지하고 있는 것으로 나타났다. AMOVA 분석 결과 전체 유전변이의 약 16%가 집단 간 차이에 기인하는 것으로 설명되었으며, 나머지 84%는 집단 내 개체간에 존재하는 것으로 나타났다. 이처럼 제한된 분포범위를 가지는 꼬리말발도리 집단에서 나타나는 높은 유전다양성과 집단간 낮은 유전적 분화율은 완전 타가수정하는 교배양식과 집단내 비교적 풍부한 개체수의 영향인 것으로 판단된다. 따라서 현재의 유전다양성을 유지할 수 있는 적절한 현지 내 보전대책 수립이 요구된다.

• 한국환경과학회지
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• 제22권7호
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• pp.873-884
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• 2013

This study was carried out to establish the conservation plan of specific plants by surveying and analyse the individuals and the environment of habitats on specific plants designated by Juwangsan national park office. The specific plants were 14 species including Berchemia berchemiaefolia, Hylotelephium ussuriense, Aristolochia manshuriensis, Lilium cernuum, Thalictrum coreanum, Exochorda serratifolia, Lilium distichum, Aristolochia contorta, Jeffersonia dubia, Eleutherococcus senticosus, Salix chaenomeloids, Eranthis stellata, Scopolia japonica, and Paeonia japonica. The habitats of specific plants were 40 sites including Berchemia berchemiaefolia of 11 sites, Hylotelephium ussuriense of 8 sites, Lilium cernuum and Thalictrum coreanum of each 3 sites, Exochorda serratifolia of 2 sites, and Lilium distichum, Aristolochia contorta, Jeffersonia dubia, Eleutherococcus senticosus, Salix chaenomeloids, Eranthis stellata, Scopolia japonica and Paeonia japonica of each 1 site. The altitude was the most in 300~400 m. In direction, NW was the most, and it showed SW in that order. The slope angle was the most in 0~10%, and the cliff showed in that order. In the results of species composition by stratum layer, Berchemia berchemiaefolia had the most, 15 species, in tree layer. In shrub and herb layer, this had the most as 20 species and 29 species, too. The species that occupied the largest size was Salix chaenomeloids in 2,500 $m^2$, and Jeffersonia dubia showed 1,500 $m^2$ in that order. The conservation of specific plants is to accomplish the ex situ and in situ conservation at the same time.

• 한국한의학연구원논문집
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• 제14권3호
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• pp.49-56
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• 2008

Glehnia littoralis Fr. Schmidt et Miquel is an important medicinal plants in East Asian countries. This plant species naturally distributed in Korea, Japan, China, and Taiwan, but it is a rare plants living in the coastal dune in Korea. To investigate the genetic variation, genetic diversity and genetic evolutionary relationships of 14 different geographical G. littoralis, ITS sequence and random amplified polymorphic DNA (RAPD) were analyzed. On the basis of ITS sequences, it was clearly showed that the ITS1 and ITS2 sequences among 14 populations are identical regardless of geographical origin excepting 2 bp in pair-wise comparison of ITS1. Furthermore, RAPD results also showed that 14 different geographical G. littoralis produce various polymorphic patterns without critical relationship among neighboring regions. These combined results suggest that the geographical variation and genetic evolution of G. littoralis is stable and provide important information on genetic diversity, and conservation of this rare plant species in situ and ex situ.

• 식물분류학회지
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• 제43권3호
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• pp.236-243
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• 2013

북방계 식물들의 잔존적 피난처인 아고산대에 분포하는 식물들은 기후변화에 따른 자생지 환경변화로 인해 멸종 및 멸절 위험에 크게 직면해 있는 실정이다. 본 연구는 한반도의 남부지역 아고산대에 제한적으로 분포하고 있는 특산식물이며 희귀식물인 설앵초(Primula farinosa subsp. modesta (Bisset & Moore) Pax)에 대한 유전적 다양성과 지리적 분화 양상을 구명을 통한 보존전략을 수립하기 위해 3지역 6집단 165개체를 대상으로 ISSR (Inter Simple Sequence Repeat) 분석을 수행하였다. 그 결과 집단 수준의 유전 다양성의 평균은(P = 60.62, SI = 0.299, h = 0.190) 유사한 생활사를 갖는 다년생 초본류의 평균보다 낮은 수준이었으며, 이형화주의 특징을 갖는Primula속의 근연 분류군들에 비해서도 다소 낮은 결과를 보였다. 유전적 분화도 구명을 위해 AMOVA(Analysis of molecular variance) 분석 결과 전체 유전변이 중 약 50%가 지역 간에 분포하는 결과를 보여 종내 지역간 분화도가 매우 높은 수준으로 판단되며, Bayesian cluster 분석 결과에서도 조사된 세 지역이 각각 독특한 유전적 cluster를 형성함으로써 개체군간 유전자 이동이 매우 제한적임을 암시하였다. 따라서 설앵초 집단의 급속한 분획화와 낮은 수준의 유전적 다양성, 지역간 높은 분화도 등의 특성들을 고려하여 설앵초 집단의 개체군 감소 및 멸절 방지를 위한 현지 내 외 보전 전략 수립이 시급한 것으로 판단된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.11-11
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• 2020

Background : The genus Cypripediums are typically adapted to the low temperature and cold climates of the high land in Korea. The species which were reported in Korean peninsula are C.japonicum, C.macranthos, C.calceolus and C.guttatum. We had already reported the successful germination of the C.macranthos and C.guttatum. The small spotted lady's slipper orchid(C.guttatum) is most endangered plants to extinction of which population was reported less than 100 in Korea. The objective of the present research is restoration of the C.guttatum in ex situ and adaptation in high land and nursery. Methods and Results : The 350 seedlings of C.guttatum were transplanted in soil at alpine area(altitude 750m) and the nursery of Korea National Arboretum(KNA) in YangPyeong city on May 2019 and 2020 in order to verify the possibility of ex situ restoration. The sprouted ratio of the seedlings were less than 10% in the alpine area and 83% in the nursery of KNA. The reasons of the diverse survival ratio are presumed as the low moisture content in the soil and heavy shade of the alpine area. The 15 cypripedium species and 29 hybrids were also effectively adapted in the KNA rare plant garden. Conclusion : These results demonstrated the difficulties of the restoration for small spotted lady's slipper orchid in the natural habitation. From the above results, we may conclude that the critical factors of the genus Cypripedium are intensive care for the fragile seedlings during the 1^st year acclimatization.

• Parasites, Hosts and Diseases
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• 제48권1호
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• pp.67-69
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• 2010

Trypomastogotes of Trypanosoma brucei were detected from 4 asymptomatic kudus (Tragelaphus strepsiceros) on a game ranch located approximately 45 km north east of Lusaka, Zambia. Blood smears examined from 14 wildlife species comprising of the impala (Aepyceros melampus), Kafue lechwe (kobus leche kafuensis), sable antelope (Hippotragus niger), tsessebe (Damaliscus lunatus), warthog (Phacochoerus aethiopicus), puku (Kobus vardoni), zebra (Equus burchelli), waterbuck (Kobus ellipsiprymnus), bushbuck (Tragelaphus scriptus), reedbuck (Redunca arundinum), wilderbeest (Connochaetes taurinus), hartebeest (Alcephelus lichtensteini), African buffalo (Syncerus caffer), and kudu (Tragelaphus strepsiceros) showed that only the kudu had T. brucei. Although game ranching has emerged to be a successful ex-situ conservation strategy aimed at saving the declining wildlife population in the National Parks, our findings suggest that it has the potential of aiding the re-distribution of animal diseases. Hence, there is a need for augmenting wildlife conservation with disease control strategies aimed at reducing the risk of disease transmission between wildlife and domestic animals.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.191-198
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• 2008

Germplasm characterization and evolutionary process in viable populations are important links between the conservation and utilization of plant genetic resources. Here, an investigation is made, based on molecular and biochemical techniques for assessing and exploiting the genetic variability in germplasm characterization of taro, which would be useful in plant breeding and ex situ conservation of taro plant genetic resources. Geographical differentiation and phylogenetic relationships of Indian taro, Colocasia esculenta (L.) Schott, were analyzed by random amplified polymorphic DNA (RAPD) and isozyme of seven enzyme systems with specific reference to the Muktakeshi accession, which has been to be proved resistant to taro leaf blight caused by P. colocasiae. The significant differentiations in Indian taro cultivars were clearly demonstrated by RAPD and isozyme analysis. RAPD markers showed higher values for genetic differentiation among taro cultivars and lower coefficient of variation than those obtained from isozymes. Genetic differentiation was evident in the taro accessions collected from different regions of India. It appears that when taro cultivation was introduced to a new area, only a small fraction of genetic variability in heterogeneous taro populations was transferred, possibly causing random differentiation among locally adapted taro populations. The selected primers will be useful for future genetic analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers identified in the DNA fingerprinting study will be useful for screening a segregating population, which is being generated in our laboratory aimed at developing a taro genetic linkage map.

• 수산해양교육연구
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• 제28권3호
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• pp.861-871
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• 2016

The coastal water of Korean Peninsula is the major habitat for finless porpoises. However, hundreds of finless porpoises are currently caught incidentally by stow-net fishery every year. To prevent the mass mortality of finless porpoises and conserve marine animals, Busan Aquarium has been designated as a "Marine Life Ex-Situ Conservation Institution and Marine Animal Conservation Center," and Cetacean Research Institute, under the National Institute of Fisheries Science have started RRR (rescue, rehabilitation and release) project together since 2011. From 2012 to 2013, four individuals of finless porpoises had been rescued and two of them finally released to their original habitat after fully recovered in July 2013. As a result, according to growth curve of finless porpoise of Korea more than 140 cm of body length, 38 kg of total weight referred to adult, results of observation of the change in the hormone revealed that mating time of finless porpoises is January, Jun and July. To become the initial institution which rescues, treatment, husbandry and releases finless porpoise in Korea.