• 제목/요약/키워드: Ethylalcohol

검색결과 26건 처리시간 0.018초

수종 고정액의 상피세포에 대한 고정효과에 관한 연구 (A Study on the Fixing Effects of Several Fixatives on Oral Epithelial Cell)

  • 홍란
    • Journal of Oral Medicine and Pain
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    • 제3권1호
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    • pp.23-27
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    • 1977
  • The author thought that the results of cytologic tests could be changed according to the fixative methods. So the author has studied several methods of fixing exfolaiative cells collected from normal adult cheek mucosa with a solution of equal parts of ether and 95% ethylalcohol, 95 ethylalcohol and Isoprophyl alcohol, and compared the results with each other. The results were as follows : The effects of fixation, and staining efficiency of nucleus and protoplasm are similar in each method, but the staining efficiency of cytoplasm and the conversation of cytoplasmic membrane were best in 95% ethylalcohol.

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고정화 효모를 충진한 column형 reactor에 의한 정어리 어간장의 속성 연속발효 (Continuous Rapid Fermentation of Sardine Soy Sauce by Using Column Type Reactor Packed Immobilized Yeast Cells)

  • 김성준;신동분;류병호
    • 한국식품과학회지
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    • 제25권2호
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    • pp.154-159
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    • 1993
  • 본 연구는 간장의 속성발효를 하기 위하여 Pediococcus halophilus R-22, Saccharomyces rouxii R-60과 Candida etchellsii H-50의 균체를 고정화하여 충진한 column형 reactor에 정어리 분해액을 주입하면서 연속적으로 속성발효를 시도하였다. Column형 reactor에서 P. halophilus R-22의 고정화 균체의 연속적 속성 발효시 유산의 생성은 발효 25일까지는 $0.62{\sim}0.64%$이었고 30일 이후부터는 약간 감소하였다. S. rouxii R-60 고정화 체의 연속적 속성 발효시 시작일부터 35일까지는 ethylalcohol이 $2.1{\sim}2.5%$로 거의 일정하게 생성되었고 40일 이후에는 약간 감소하였다. C. etchellsii H-50 고정화 균체의 발효에서는 35일까지는 4-ethylguaiacol의 함량이 $14{\sim}16\;mg/l$로 거의 일정하게 생성되었으나 발효 40일 이후 약간 감소하였다. Column형 reactor의 발효에서 얻은 어간장의 성분을 분석한 결과 총질소는 1,721.6 mg%, 아미노태 실소는 1,584.1 mg%, 유산은 0.75%, ethylalcohol은 2.7%, 4-ethylguaiacol은 18.2 mg/l이었다.

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Candida Utilis 효모를 이용한 HVP 특유의 향 생성 최적화 (Optimization of HVP-flavour formation using Candida utilis)

  • 박근형;이재화;김의용;채희정
    • 한국산학기술학회:학술대회논문집
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    • 한국산학기술학회 2004년도 춘계학술대회
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    • pp.307-310
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    • 2004
  • 본 연구에서는 분리대두단백(ISP: isolated soy protein)과 탈지대두박(DSF: defatted soy flake)을 Devolase Flavourzyme 효소로 가수분해한 각각의 HVP를 발효 배지로 사용하여 Candida utilis(KCCM 50342) 효모의 ethylalcohol, 4-ethylguaiacol의 생성과 향 생성의 주요 인자인 a -galactosidase의 활성을 측정하였다. Ethylalcohol은 발효 1일째에 탈지대두박을 원료로 하고 여과후 미생물 처리한 경우에서 가장 높게 생성되었고, 4-ethylguaiacol은 일부 여과하지 않고 미생물 처리한 경우에서 낮은 농도의 생성을 보였다. a-galactosidase 효소활성은 탈지대두박 보다 ISP를 원료로 한 HVP에서 높은 활성을 보였다. 관능검사결과 탈지대두박을 사용한 HVP의 관능적 특성이 ISP를 원료로 한 HVP보다 더 우수하였다. 결과적으로 탈지대두박을 원료로 한 HVP의 향기성분 및 관능적 특성이 우수함을 확인하였다.

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고정화균체를 이용하여 속성 발효시킨 정어리 어간장의 젖산, 알코올 및 4-ethylguaiacol의 함량 (Lactic Acid, Ethylalcohol and 4-Ethylguaiacol Contents of Rapid Fermentation of Sardine Soy Sauce Prepared by Using Immobilized Whole Cells)

  • 류병호;김성준;신동분
    • 한국식품과학회지
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    • 제24권5호
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    • pp.456-462
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    • 1992
  • 본 연구는 간장의 속성발효를 위한 방법의 하나로 정어리육을 효소에 의하여 분해하고, 분해액을 유산균과 효모의 균체를 고정화하여 이를 column형 reactor를 이용하여 속성발효를 시도하였다. 이때 사용된 column 반응조는 유리제$(30{\times}50cm)$의 칼럼 3개에 Pediococcus halophilus R-22, Saccharomyces rouxii R-60과 Candida etchellsii H-50을 colloidal silic와 sodium alginate(1 : 5)의 혼합액으로 고정화하여 각각의 column에 충전시켰다. 이때의 최적조건은 pH5.2, 온도 $30^{\circ}C$, 식염농도 10%이었다. 발효 최적조건에서 96시간 경과시 P. halophilus R-22는 유산을 0.75%, S. rouxii R-60은 ethylalcohol을 2.5%, C. etchellsii H-50은 4-ethylguaiacol을 18 mg/l 생성하였다.

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용액과 용융결정화에 의한 나프탈렌과 2-메틸나프탈렌 혼합물로부터 나프탈렌의 분리에 관한 연구 (A Study on Separation of Naphthalene from Naphthalene and 2-Methylnaphthalene Mixture by Melt and Solution Crystallization)

  • 김성일;정광은;채호정;정순용;김철웅
    • 한국응용과학기술학회지
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    • 제25권2호
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    • pp.232-239
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    • 2008
  • Separation of naphthalene from naphthalene and 2-methylnaphthalene mixture has been studied by layered melt and solution crystallization using ethylalcohol. Purity and yield of naphthalene depended mainly on the cooling rate: The effective distribution coefficient ($K_{eff}$) as the degree of impurity removal was observed to decrease with the decreasing in cooling rate. Purity of naphthalene can be enhanced to $5{\sim}7%$ by melt crystallization using 90% naphthalene and the purity of naphthalene can be obtained to be 99% up by solution crystallization.

Dehydrogenation of Ethylalcohol Catalyzed by Alcoholdehydrogenase Under High Pressure

  • 지종기;신진영;황정위
    • Bulletin of the Korean Chemical Society
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    • 제10권1호
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    • pp.50-57
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    • 1989
  • A pressure effect of the dehydrogenation of ethylalcohol catalyzed by alcoholdehydrogenase was observed in Tris-HCl buffer, pH 8.8 from $25^{\circ}C$ to $35^{\circ}C$ under high pressure system by using our new theory. The theory makes it possible for us to obtain all rate and equilibrium constants for each step of all enzymatic reaction with a single intermediate. We had enthalpy and volume profiles of the dehydrogenation to suggest a detail and reasonable mechanism of the reaction. In these profiles, both enthalpy and entropy of the reaction are positive and their values decrease with enhancing pressure. It means that the first step is endothermic reaction, and its strength decrease with elevating pressure. At the same time, all activation entropies have large negative values, which prove that not only a ternary complex has a more ordered structure at transition state, but also water molecules make a iceberg close by the activated complex. In addition to this fact, the first and second step equilibrium states are controlled by enthalpy. The first step kinetic state is controlled by enthalpy but the second step kinetic state is controlled by entropy.

야관문이 생쥐의 면역세포 활성에 미치는 영향 (Effect of Lespedeza Cuneata G. Don on the Activity of Murine Immune Cells)

  • 은재순
    • 동의생리병리학회지
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    • 제25권5호
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    • pp.837-842
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    • 2011
  • The purpose of this research was to investigate the effects of 50% ethylalcohol extracts of Lespedeza cuneata G. Don (LE) on the activity of murine immune cells. LE was administered orally once a day for 7 days at the dose of 250 mg/kg. LE increased the cell viability of thymocytes, splenocytes and peritoneal macrophages in vitro and in vivo system, but decreased the cell viability of thymocytes and splenocytes in the presence of concanavalin A in vivo system. Also, the administration of LE was increased the production of ${\gamma}$-interferone, but did not affect the production of interleukin-2 and interleukin-4. Furthermore, LE decreased the phagocytic activity of peritoneal macrophages in vitro and in vivo system, but enhanced the production of nitric oxide. These results suggest that LE has a immuno-regulative action via stimulation of immune cells proliferation.

유기성 폐수의 오존전해처리에 관한 연구 (A Study on the Treatment of Organic Wastewater by Ozone Electrolysis)

  • 정홍기;이태호
    • 환경위생공학
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    • 제11권2호
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    • pp.59-63
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    • 1996
  • To treat certain wastewater that has alcohol and phenol, we performed the ozone electrolysis by using the titanium electrode. In this experiment, we examined decomposition voltage of organics, time for electrolysis, and removal efficiency of organics. In addition we compared the ozone oxidation electrolysis. The followings are results; 1. When it comes to the alcohol treatment in wastewater, ozone electrolysis showed higher removal efficiency than ozone oxidation or electrolysis. 2. After comparing the decomposition rate of methylalcohol, ethylalcohol, and prophylalcohol in ozone electrolysis, we knew the fact that increasing carbon number made the decomposition rate slow. 3. According to the treatment of alcohol by ozone electrolysis, decomposition voltage was 50V, time for electrolysis was three hours, and treatment acidity was neutral (pH 6.5 - 8.1). 4. Ozone electrolysis was effective to the phenol treatment. When we treated phenol by using ozone electrolysis for three hours, TOC treatment efficiency was 95%. However, ozone oxidation just showed 45% treatment efficiency.

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알코올을 이용한 흉부교감신경절차단술에 대한 임상경험 및 합병증 -증례 보고- (The Clinical Experiences and Complications of Percutaneous Neurolysis of Upper Thoracic Sympathetic Ganglion by Using Ethylalcohol -A report of three cases-)

  • 권옥희;김종일;반종석;민병우
    • The Korean Journal of Pain
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    • 제8권2호
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    • pp.374-377
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    • 1995
  • Percutaneous neurolysis of upper thoracic sympathetic ganglion was performed by simultaneously injecting 3 ml of pure alcohol into the $T_2$ and $T_3$ levels after testng with same amount of local anesthetics on the same sites. We experienced poor sympatholytic effect or intercostal neuritis and Horner's Syndrome as the result of complication of thoracic sympathetic ganglion block. In Case 1, in spite of the good testing result, neurolytic block effect was poor. In Case 2, intercostal neuritis occurred, but neuralgia subsided within 3 weeks. In Case 3, Horner's Syndrome occurred for 1 day. To increase the success rate of block and decrease the incidence of complications, good radio-opaque dye appearance and good test block effect should be obtained.

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Design of Thin solid Film Systems for Nd High Power Laser and Sol-gel Coating Experiment

  • Kim, Hyeon-Soo
    • 한국광학회:학술대회논문집
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    • 한국광학회 1989년도 제4회 파동 및 레이저 학술발표회 4th Conference on Waves and lasers 논문집 - 한국광학회
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    • pp.49-54
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    • 1989
  • Thin solid film systems polarizing beam splitter and disk amplifier used in the high power laser system ({{{{ lambda }}0=1060nm) are designed by computer aided optimization technique. Extinction ratio of designed polarizing beam splitter for incidence angle 30$^{\circ}$, 45$^{\circ}$, 55.60$^{\circ}$ are 1:93, 1:895, 1:1991. respectively. Maximum reflectance of designed thin film system of disk amplifier is less than 3% for pumping band (500-900nm) and 0.15% for laser beam. Further, SiO film chosen as one of the suitable thin films in the high power laser system is prepared by the sol=-gel process which gives high damage threshold. When the withdrawal speed is 6.15cm/min -16.62 cm/min and the mixing ratio is in the range of 1 mol (ethylsilicate): 4-8mol(ethylalcohol) : 2mol(water), the thickness of deposited film is in the range of 500{{{{ ANGSTROM }}-1500{{{{ ANGSTROM }}.

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