• Title/Summary/Keyword: Enlarged shape

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Chondrocyte Culture from Epiphyseal Plate and its Morphological Changes in Autologous Implants of Rabbit (토끼 성장판 연골세포 배양과 자가 이식편에서의 형태학적인 변화)

  • 양영철;정해일;최장석
    • Journal of Life Science
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    • v.10 no.4
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    • pp.408-421
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    • 2000
  • We tried to establish the culture method of the chondrocyte isolated from the epiphyseal cartilage and to investigate morphological changes of chondrocyte cultured with enzyme-digested costal cartilage, the perichondrium and experimentally damaged meniscus of rabbit. De novo chondrocyte pellets were prepared from epiphyseal plates by culturing isolated epiphyseal chondrocytes from 4 week. old rabbits. We morphologically assessed the cartilage formation of the chondrocyte culture with enzyme-digested costal carilage, the perichondrial culture, the cultured chondrocytes transplants into experimentally damaged meniscus of rabbits, the perichondrial culture, the cultured chondrocytes transplants into experimentally damaged meniscus of rabbit. In the 24 days, the epiphyseal chondrocytes maintained the typical phenotypes of the partial nodular cell formation. The 30 days cryopreserved chondrocytes showed abnormal and irregular shape. In the type II collagen added culture, the chondrocytes showed expanded rough endoplasmic reticulum and small and large round-like vesicles of processes. In the type IV collagen added culture, the chondrocytes showed large perinuclear vaculoes and abundant well-developed rough endoplasmic reticulum of processes. In the culture with enzyme- digested costal cartilage and the perichondrial culture, the chondrocytes showed a few swelling rough endoplasmic reticulum and vacuoles. The cultured epiphyseal chondrocytes maintained typical phenotype and the chondrocytes were grown faster and maintained more typical phenotype in the type II and IV collagen added culture. The transformed chondrocytes secreted abundant extracellular matrix in the type II collagen added culture, and showed processes in the type IV collagen added culture. The perichondrial chondrocytes were grown faster and their implants were able to transplant. The cultured chondrocytes transplanted into experimentally damaged meniscus were adapted between the meniscus tissues. And the immunocyto-chemical reaction of the type II collagen of the chondrocytes were found to be maintained. The chondrocytes cultured cartilage. The chondrocytes secreted abundantly. The cultured chondrocytes transplanted into experimentally damaged meniscus changed immature cells into enlarged mature cells with extracellular secretion.

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Actual Wearing Conditions and Fitting Problems of Ready-to-wear Garment for Tween Generation Boys aged from 12 to 14 (만(滿) $12{\sim}14$세(歲) 트윈 세대(世代) 남학생(男學生)의 의복(衣服) 착용실태(着用實態) 및 맞음새 연구(硏究))

  • Kim, Kyung-A
    • Journal of Fashion Business
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    • v.8 no.5
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    • pp.85-99
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    • 2004
  • The purpose of the study is to survey the current state of tween generation boys' clothing-wearing, to identify problems in the current apparel sizing system, and to contribute to the improvement of the fitness of ready-made clothes. 1. According to the result of surveying the current state of clothing-wearing, boys prefer casual wear and, as they grow old, they tend to purchase clothes alone or in company with their friends. In addition, they use mainly easy casual wear or sports wear stores. They appear to be highly dissatisfied with price and size and think that the fitting of coats, pants and jackets is poor. Moreover, they complain about sizes such as waist circumference, pants length and upper-arm circumference. 2. According to the result of comparing apparel sizing system with body measurements, the waist circumference and hip circumference of young casual wear are fit to the large size of body at the age of 12 and the over-average size of body at the age of 13 and 14, but its bust circumference is fit only to the extra-large size of body at the age of 13 and 14. Such results come from differences in body shape between adults and adolescents. Although adolescents' body size has been enlarged, their body line is still immature and, accordingly, their drop-value is smaller than that of adults, which appears to cause adolescents to be highly dissatisfied with fitting.

Process Conditions for the Fabrication of Hydrophobic Surfaces with Different Photo-curable Resins (광경화성 레진의 성분 변화에 대한 소수성 표면 제작을 위한 공정 조건)

  • Hong, Sung-Ho;Woo, Heung-Sik
    • Tribology and Lubricants
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    • v.36 no.5
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    • pp.267-273
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    • 2020
  • This study experimentally investigates hydrophobic surfaces fabricated via additive manufacturing. Additive manufacturing, commonly known as 3D printing, is the process of joining materials to fabricate parts from 3D model data, usually in a layer-upon-layer manner. Digital light processing is used to fabricate hydrophobic surfaces in this study. This method uses photo-curable resins and ultraviolet (UV) sources. Moreover, this technique generally has faster shaping speeds and is advantageous for the fabrication of small components because it enables the fabrication of one layer at a time. Two photo-curable resins with different compositions are used to fabricate micro-patterns of hydrophobic surfaces. The resins are composed of a photo-initiator, monomer, and oligomer. Experiments are conducted to determine suitable process conditions for the fabrication of hydrophobic surfaces depending on the type of resin. The most important factors affecting the process conditions are the UV exposure time and slice thickness. The fabrication capability according to the process conditions is evaluated using the side and top views of the micro-patterns observed using a microscope. The micro-patterns are collapsed and intertwined when the exposure time is short because sufficient light (heat) is not applied to cure the photo-curable resin with a given slice thickness. On the other hand, the micro-patterns are attached to each other when the exposure time is prolonged because the over-curing time can cure the periphery of a given shape. When the slice is thicker, the additional curing area is enlarged in each slice owing to the straightness of UV light, and the slice surface becomes rough.

Pectinase-Processed Ginseng Radix (GINST) Ameliorates Hyperglycemia and Hyperlipidemia in High Fat Diet-Fed ICR Mice

  • Yuan, Hai-Dan;Kim, Jung-Tae;Chung, Sung-Hyun
    • Biomolecules & Therapeutics
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    • v.20 no.2
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    • pp.220-225
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    • 2012
  • To develop a ginseng product possessing an efficacy for diabetes, ginseng radix ethanol extract was treated with pectinase and obtained the GINST. In the present study, we evaluate the beneficial effect of GINST on high fat diet (HFD)-induced hyperglycemia and hyperlipidemia and action mechanism(s) in ICR mice. The mice were randomly divided into five groups: regular diet group (RD), high fat diet group (HFD), HFD plus GINST at 75 mg/kg (GINST75), 150 mg/kg (GINST150), and 300 mg/kg (GINST300). Oral glucose tolerance test reveals that GINST improves the glucose tolerance after glucose challenge. Fasting plasma glucose and insulin levels were decreased by 4.3% and 4.2% in GINST75, 10.9% and 20.0% in GINST150, and 19.6% and 20.9% in GINST300 compared to those in HFD control group. Insulin resistance indices were also markedly decreased by 8.2% in GINST75, 28.7% in GINST150, and 36.4% in GINST300, compared to the HFD control group. Plasma triglyceride, total cholesterol and non-esterified fatty acid levels in the GINST300 group were decreased by 13.5%, 22.7% and 24.1%, respectively, compared to those in HFD control group. Enlarged adipocytes of HFD control group were markedly decreased in GINST-treated groups, and shrunken islets of HFD control mice were brought back to near normal shape in GINST300 group. Furthermore, GINST enhanced phosphorylation of AMP-activated protein kinase (AMPK) and glucose transporter 4 (GLUT4). In summary, GINST prevents HFD-induced hyperglycemia and hyperlipidemia through reducing insulin resistance via activating AMPK-GLUT4 pathways, and could be a potential therapeutic agent for type 2 diabetes.

Design Optimization for Air Ducts and Fluid Pipes at Electromagnetic Pulse(EMP) Shield in Highly Secured Facilities (EMP 방호시설의 덕트 및 배관 최적 설계 방안)

  • Pang, Seung-Ki;Kim, Jae-Hoon
    • Journal of the Korean Society for Geothermal and Hydrothermal Energy
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    • v.10 no.4
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    • pp.15-24
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    • 2014
  • This study conducted a computational fluid dynamics(CFD) analysis to find an appropriate diameter or sectional area of air ducts and fluid pipes which have an electromagnetic pulse(EMP) shied to protect indoor electronic devices in special buildings like military fortifications. The result shows that the optimized outdoor air intake size can be defined with either the ratio of the maximum air velocity in the supply duct to the air intake size, or the shape ratio of indoor supply diffuser to the outdoor air intake. In the case of water channel, the fluid velocity at EMP shield with the identical size of the pipe, decreases by 25% in average due to the resistance of the shield. The enlargement of diameter at the shield, 2 step, improves the fluid flow. It illustrated that the diameter of downstream pipe size is 1step larger than the upstream for providing the design flow rate. The shield increases friction and resistance, in the case of oil pipe, so the average flow velocity at the middle of the shield increase by 50% in average. In consideration of the fluid viscosity, the oil pipe should be enlarged 4 or 5 step from the typical design configuration. Therefore, the fluid channel size for air, water, and oil, should be reconsidered by the engineering approach when EMP shield is placed in the middle of channel.

Influence of cingulate cortical ablation and vagotomy upon gastric ulceration in rats (흰쥐의 위궤양 발생에 미치는 대상회전 제거 및 미주신경 절단의 영향)

  • Park, Hyoung-Jin;Kang, Sook-Hee;Jo, Yang-Hyeok;Kim, Chul
    • The Korean Journal of Physiology
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    • v.11 no.1
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    • pp.45-50
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    • 1977
  • This study was undertaken to investigate the effect of cingulate cortical ablation upon gastric ulceration, and the pathway through which cingulate cortex exerts the effect. 56 female rats were divided equally into cingulate (cingulate cortical ablation), cingulate-vagal (cingulate cortical ablation and vagotomy), normal control and vagal (vagotomy) groups. Cingulate cortex was ablated through a slit-shaped opening (1 mm in width, 13 mm in length) which was made symmetrically on both sides of, and parallel to, the sagittal suture by removing a bone flap from parietal and frontal bones on each side. Vagus nerves on both sides were transected around the distal end of the esophagus. In the normal control animals, surgical intervention ended with scalp incision. All rats were kept without restraint or food deprivation for 3 weeks after surgery. The stomach of each rat was inflated with 7 ml of physiological saline and then removed under deep anesthesia. The mucosal surface was examined under dissecting microscope for the location, shape and number of ulcers, and then enlarged photograph $(4{\times})$was taken. The incidence of ulcer in each group was counted and the number of ulceration as well as the total area of glandular mucosa were measured on the photograph. Results obtained were as follows: 1. The mean number of ulcer per stomach and the total area of ulcer exprssed as permillage of the total area of glandular mucosa were significantly higher in the cingulate group than the cingulate-vagal, the normal control and the vagal groups. There was no difference among the latter three groups. 2. The incidence of ulcer in the cingulate group was significantly higher than that in the .normal control group and was also higher, though not significantly, than those in the cingulate-vagal and the vagal groups. There was no difference among the normal control, the cingulate-vagal and the vagal groups. It is inferred from the above results that the cingulate cortex exerts an inhibitory influence upon gastric ulceration and that this influence is mediated by controlling the vagal activity.

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A Study on the Dwellings of Korean Diaspora in Russia and Central Asia (옛 소련 지역 한국인 동포의 주거건축에 관한 연구 - 단독주택 평면을 중심으로 -)

  • Lee, Sang-Hae
    • Journal of architectural history
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    • v.13 no.4 s.40
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    • pp.19-34
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    • 2004
  • This study examines the dwellings of the Korean diaspora in Maritime Provinces of Russia, and Kazakhstan and Uzbekistan of Central Asia to find the gist of the residence transformed as Korean traditional residence culture to accept Russian foreign culture. Through the examination, transformation process of the dwellings of the Korean diaspora was found as follows: - 1st Period(1864-1937): The Korean diaspora who Immigrated to Maritime Provinces of Russia built traditional houses of Korean style and few of them lived in Russian style houses. - 2nd Period(1937-1955): The Koreans who immigrated to Central Asia from Maritime Provinces under compulsion built 'ground house' by digging the earth and installed gudeul which is a traditional Korean heating system and roofed with reed. - 3rd period(1955-1991): The Koreans built straight lined '-' shape houses with two or three rooms wherein most of them were installed with gudeul to heat the room. Around the 1940s as they economically got well, the Koreans started to build houses with Russian style with one or two rooms with gudeul or a separate building with gudeul. - 4th period(1991-present): Houses of the 3rd period are still used by being enlarged or remodeled with less use of gudeul which is well reflected on Ujeong-maul village in Russia Maritime Provinces. As can be found above, the dwellings of the Korean diaspora in Russia Maritime Provinces and Central Asia are on the process of being transformed into Russian style to adapt to Russian culture.

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A Study on the Improvement of Efficiency and Linearity of Power Amplifier using PBG Structure (PBG 구조를 이용한 전력 증폭기의 효율 및 선형성 개선에 관한 연구)

  • 김병희;박천석
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.12 no.7
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    • pp.1182-1190
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    • 2001
  • In this paper, microstrip photonic bandgap (PBG) structure with special perforation patterns etched on the line itself is analyzed and optimized in shape, then used for harmonic tuning of power amplifier. This PBG has an advantage in being fabricated and grounded. The dimension of unit lattice is enlarged vertically, but its input and output line maintain 50 Ω using tapered line. This modification from original structure can lessen possible error in etching PCB. The analysis and design of PBG structure are acquired from using EM simulation. The measured insertion loss of the final structure is 0.3 ∼0.4 dB, and its bandwidth of stopband is 6∼7 GHz. Measured results of improved characteristics by using PBG structure at the output of the power amplifier are 0.72∼0.99 dB in output power, 1.14∼7.8 % in PAE, and 1 dBc in the third IMD.

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Ultrastructural Changes of Oocytes from Vitellogenesis into Maturation in Korean Catfish, Silurus asotus (난황형성부터 성숙까지 메기(Silurus asotus) 난모세포의 미세구조)

  • Yoon, Jong-Man;Kim, Young-Gill
    • Applied Microscopy
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    • v.28 no.4
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    • pp.447-463
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    • 1998
  • This study was carried out to investigate the histomorphological changes and the electrophoretic patterns of egg components, obtained from 100 of 2-year-old female catfishes (Silurus asotus). Female Korean catfishes collected in the vicinity of Chollabukdo have synchronous ovaries which discontinously ovulate eggs during the breeding season (from late May until early July). The fishes were killed by severing the spinal cord just posterior to the head after immobilization with tricaine methanesulfonate (MS 222). Especially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were examined by means of light and transmission electron microscopy. The size of the nucleoli and number of the yolk granules increased as the oocyte groved. Yolk granules were deposited in the oocyte as fluid Due to tile presence of large early and late maturing oocytes, their ovaries were enlarged, transparent, granular and greenish in color. As the percentages of fish in late maturing oocyte (LMO) and ripe oocyte (RO) stage increased from March to April, mean gonadosomatic index (GSI) values (19.95%) increased. Zona radiata changed from squamous into cuboid in shape in the early maturing oocyte (EMO) stage. Processes, microvilli, from the zona radiata and from the oocyte grow, and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radials becomes more compact and devoid of pore canals during oocyte maturation. Seasonal changes in the microscopic appearance of the ovaries were well correlated with those in both gonadosomatic index and macroscopic appearance. The main cytological changes such as increase in size of cell, nucleus, nucleolus, and increase in number of nucleoli and mitochondria demonstrated with electron microscopy in the previtellogenic oocytes of Korean catfish, provided evidences for important synthetic processes in an early preparatory phase of oocyte development. The electrophoretic pattern of major band in mature stage was much thicker (24 k, 66 k, 90-110k dalton) than that in previtellogenic phase.

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Reconsideration on the Origination and Changes of Changbang-anchogongs in Joseon Period (조선 시대 창방안초공의 기원과 변천 재고찰)

  • Lee, Woo-Jong
    • Journal of the Architectural Institute of Korea Planning & Design
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    • v.34 no.9
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    • pp.129-140
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    • 2018
  • The purpose of this study was to reconsider existing hypotheses on the orinination and changes of changbang-anchogongs and their types and suggest an alternative hypothesis. The earliest changbang-anchogongs in $17^{th}$ century is presumed to originate as imitations of chungbang ppaelmoks for ornamental and reinforcing parts for changbangs. They were framed whole sides of buildings equally, and were used in small numbers of extant royal buildings and Buddhist halls. From late $17^{th}$ century, enlarged dragon shape changbang-anchogongs applied to Buddhist halls maily to decorate and strengthen front side of buildings. As a result of exchange between changbang-anchogongs and Ikgong type brackets, combined changbang-anchogongs in Buddhist halls were invented for stronger connection with upper bracketing units and have been spread during $18^{th}$ century. Danpyeongbang anchogongs were originated by the connection between short pieces of pyeongbangs and early type of anchogongs similar to ppaelmoks. They belong to minority subtype, but were drawn in illustrations by mistake, as parts of main halls of royal palaces from $19^{th}$ century, because of painter's confusion about new techniques for changbang-anchogongs. Combined changbang-anchogongs for royal buildings were presumed to be invented in 1790s by stimulation from a Buddhist hall related to royal tomb. However, unlike Buddhist precedence, they were used to reinforce and decorate whole sides, even corners, of buildings, and their frameworks and shapes were imported from Daeryang-anchogongs at flanks of ritual halls in royal tombs and royal shrines from $17^{th}$ century.