• Asian-Australasian Journal of Animal Sciences
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• v.23 no.4
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• pp.456-464
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• 2010

RNA interference (RNAi) is an acknowledged useful and effective tool to study gene function in various cells. Here, we suppressed the Connexin 43 (Cx 43) gene expression during in vitro development of ovine pre-implantation embryos using the RNAi method. The 353 bp Cx 43 double-stranded RNA was microinjected into in vitro fertilized ovine zygotes, and the levels of target mRNA and protein were investigated. Control groups included uninjected zygotes or those injected with RNase-free water. The dsRNA injection resulted in the specific reduction of Cx 43 transcripts as analyzed by quantitative real-time RT-PCR and decreased protein levels as shown by Western blot analysis at the blastocyst stage. Microinjection of Cx 43 dsRNA led to 20.3%, 21.7% and 34.5% blastocyst rates and 19.2%, 37.5% and 41.3% hatched blastocyst rates in Cx 43 dsRNA-injected, water-injected and uninjected groups, respectively. Then the RNAi could not significantly affect cell number and cell death rates of blastocysts. Therefore, suppression of Cx 43 dsRNA and proteins did not apparently affect the development potential of ovine pre-implantation embryos but may play a role in embryo quality. RNAi technology is a promising approach to study gene function in early ovine embryogenesis.

• The Mathematical Education
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• v.56 no.3
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• pp.341-363
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• 2017

This case study contributes to the efforts on identifying the essential features of responsive teaching practice where students' mathematical thinking is central in instructional interactions. We firstly conceptualize responsive teaching as a type of teachers' instructional decisions based on noticing literature, and agree on the claim which teachers' responsive decisions should be accounted in classroom interactional contexts where teacher, students and content are actively interacting with each other. Building on this responsive teaching model, we analyze classroom observation data of a 7th grade teacher who implemented a lesson package specifically designed to respond to students' mathematical thinking, called Formative Assessment Lessons. Our findings suggest the characteristics of responsive teaching practice and identify the relationship between noticing and responsive teaching as: (a) noticing on students' current status of mathematical thinking by eliciting and anticipating, (b) noticing on students' potential conceptual development with follow-up questions, and (c) noticing for all students' conceptual development by orchestrating productive discussions. This study sheds light on the actual teachable moments in the practice of mathematics teachers and explains what, when and how to support teachers to improve their classroom practice focusing on supporting all students' mathematical conceptual development.

• Journal of Korean Elementary Science Education
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• v.34 no.4
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• pp.394-405
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• 2015

The purpose of this study was to develop and apply a differentiated instruction-learning reflecting various characteristics and demands of the gifted underachievers, and thereby help to develop the study attitude and study achievement of the gifted underachievers. To achieve the purpose, a case study on five gifted underachievers who are in $6^{th}$ grade of elementary school was conducted. By collecting and analyzing a variety of data including standardized tests, interviews with teachers, students, and parents, and observation journals, this researcher examined the characteristics and demands of the gifted underachievers. For the development of the program, differentiated strategies were designed according to students' interest and concern, their multiple-intelligence, and their learning styles. As a result of the application of them, a program with a total of 20 sections was finally developed. The result of the application for the differentiated instruction-learning program revealed improvements in their academic achievements. In addition, it was found that their learning motives were improved. The program was also found to affect their self-concept and their attitude toward learning. The study showed that developing an education program for the gifted underachievers should begin from the analysis of each student's characteristics. Also, it was found that a differentiation strategy could become an alternative to apply various characteristics and demands of the gifted underachievers to the development of a program. The gifted underachievers' intellectual and definitive characteristics were changed even by the short-term mediation. Therefore, the new issue about educating the gifted is to provide a differentiated instruction-learning program fitting their characteristics and demands and help them exert their potential as best as they could.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.891-899
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• 2016

The recently isolated aniline-degrading bacterium Pseudomonas migulae AN-1 was tagged with green fluorescent protein (GFP) to investigate its bioaugmentation potential against aniline-contaminated groundwater through microcosm experiments. The survival and cellular response of GFP-tagged AN-1 introduced in a lab-scale aquifer corresponded directly with aniline consumption. During the process, the GFP-tagged AN-1 biomass increased from 7.52 × 10⁵ cells/ml to 128 × 10⁵ cells/ml and the degradation rate of aniline was 6.04 mg/l/h. GFP-tagged AN-1 was moderately hydrophobic (41.74%-47.69%) when treated with 20-100 mg/l aniline and exhibited relatively strong hydrophobicity (55.25%-65.78%) when the concentration of aniline was ≥100 mg/l. The membrane permeability of AN-1 increased followed by a rise in aniline below 100 mg/l and was invariable with aniline above 100 mg/l. Pyrosequencing analysis showed that the relative abundance of Proteobacteria (accounted for 99.22% in the non-bioaugmentation samples) changed to 89.23% after bioaugmentation with GFP-tagged AN-1. Actinobacteria increased from 0.29% to 2.01%, whereas the abundance of Firmicutes barely changed. These combined findings demonstrate the feasibility of removing aniline in aquifers by introducing the strain AN-1 and provide valuable information on the changes in the diversity of dominant populations during bioaugmentation.

• Journal of Applied Biological Chemistry
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• v.52 no.4
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• pp.216-220
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• 2009

Aldo-keto reductase family 1 B10 (AKR1B10) has been considered as a potential cancer therapeutic target. Ethanol extracts prepared from 82 Korean local plants were examined for their antioxidant activity and inhibitory effects on recombinant human AKR1B10 (rhAKR1B10) in vitro. 21 extracts showed more than 80% of ABTS radical scavenging activity at $100\;{\mu}g/mL$ and 11 extracts inhibited more than 50% of rhAKR1B10 activity at $10\;{\mu}g/mL$. Especially, 9 extracts showed potent inhibition on rhAKR1B10 activity compared with positive control tetramethylene glutaric acid.

• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1850-1858
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• 2018

Glucosamine (GlcN) is widely used in the nutraceutical and pharmaceutical industries. Currently, GlcN is mainly produced by traditional multistep chemical synthesis and acid hydrolysis, which can cause severe environmental pollution, require a long prodution period but a lower yield. The aim of this work was to develop a whole-cell biocatalytic process for the environment-friendly synthesis of glucosamine (GlcN) from N-acetylglucosamine (GlcNAc). We constructed a recombinant Escherichia coli and Bacillus subtilis strains as efficient whole-cell biocatalysts via expression of diacetylchitobiose deacetylase ($Dac_{ph}$) from Pyrococcus furiosus. Although both strains were biocatalytically active, the performance of B. subtilis was better. To enhance GlcN production, optimal reaction conditions were found: B. subtilis whole-cell biocatalyst 18.6 g/l, temperature $40^{\circ}C$, pH 7.5, GlcNAc concentration 50 g/l and reaction time 3 h. Under the above conditions, the maximal titer of GlcN was 35.3 g/l, the molar conversion ratio was 86.8% in 3-L bioreactor. This paper shows an efficient biotransformation process for the biotechnological production of GlcN in B. subtilis that is more environmentally friendly than the traditional multistep chemical synthesis approach. The biocatalytic process described here has the advantage of less environmental pollution and thus has great potential for large-scale production of GlcN in an environment-friendly manner.

• Journal of The Korean Association of Information Education
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• v.21 no.5
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• pp.567-581
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• 2017

The purpose of this study is to analyze the facilitating and conflicting factors on the emerging technologies and trends predicted to impact future education in Korea. To do this, open online questionnaires on 20 emerging technologies and trends derived from a comprehensive literature review were completed by 24 experts in research, policy, schools, and corporate fields, and a content analysis of the collected qualitative data was conducted. As a result of the study, the effectiveness of the content and the maturity of technology were found to be the most important facilitating factors and obstacles. In addition, the potential for innovative teaching and learning methods and motivation, and the maturity and popularity of technology were found to be the main facilitating factors. On the other hand, health problems and negative effects on students in ethical aspects, the lack of research and development, and poor networks and infrastructures in terms of education environment were found to be the main impeding factors of emerging technologies and trends.

• Hwankyungkyoyuk
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• v.19 no.2 s.30
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• pp.96-107
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• 2006

Inquiry has great value in environmental education(EE). Being able to see the world environmentally through 'inquiry-based environmental education' can be an important value and goal of EE. In this study, we intended to develop an EE program of measuring activity of dissolved oxygen(DO), based on the theory of 'inquiry-based EE'. Especially, we recognized the potential that DO meter can be used in 'inquiry-based EE', and we tried to develop a model of inquiry-based EE using DO meter. As a result of this research, we present specific models of inquiry-based EE about how to perform measuring activity of DO and how to use the DO meter in laboratories and streams from the perspective of inquiry of water environment. In the process of program development, we considered organization of the inquiry process, use of concept and knowledge, scientific inquiry and insightful inquiry, integration, sustain-ability, content components of 'Environmental Studies for EE', developmental level and in-forest of students. The developed EE model is a scientific inquiry model, pursuing 'explanation' based on data collection. Through this model, we tried to make students see water environment more deeply. The developed program can be applied to various water environments, like laboratories, streams, ponds, etc. It can be more effective inquiry activity if we perform measuring activities simultaneously with PH, electrical conductivity, and turbidity meters.

• The Journal of Korean Association of Computer Education
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• v.4 no.1
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• pp.161-174
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• 2001

This study is aimed at putting forward effective methods of computer education for use in schools by comparing the schools of UK and Korea. It shows : 1) a big difference in infrastructure such as H/W and Network, 2) for S/W budget UK teachers have control over their own budget, but not in Korea, 3) government, school, teacher, student, and parent all have the same mind set to promote the use of information communication technology in the UK, but in Korea the government alone drives it with its own policy. At University level, potential computer teachers in the UK are emphasized to practice fields works in schools, but in Korea, the course mainly consists of education theory and pre-teachers get only four weeks field experience. In addition, computer teachers are often those with majors in different fields, but due to the lack of interest in computer education, they are forced into these empty positions. This often leads to conflict between students and teachers because of those teachers who do not have the necessary depth in knowledge of computers.

• The Journal of the Korean dental association
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• v.57 no.12
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• pp.728-735
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• 2019

Objective: Previous studies suggested the potential influence of YouTube videos regarding dentistry on the mass population. However, there was not any clear investigation for Korean population. We aimed to systemically analyze the type of the dentistry-related videos uploaded on YouTube Korea and the accounts used for uploading, and to assess their effect on the view count of the content. Methods: Classification, type of the accounts, and view count of the videos listed by the keyword 'dentistry' were analyzed, which were uploaded on YouTube Korea platform from September 2017 to April 2019. Kruskal-Wallis test with post hoc analysis was used to assess the effect of the classification of the videos and the type of accounts on the view count. Results: 1.026 videos were enrolled to the analysis. Primary classification of the videos was information/education, advertisement, life, news, child contents, autonomous sensory meridian response, broadcast, cartoon/game, humor, and music. Secondary classification of the videos was dental experience, advertisement, role-playing, information/education., humor, cartoon/game, child contents, life, and broadcast. Type of the accounts was dentistry associates, general public, media company, and government office (sorted by frequency). Subject of the most videos (93.6%) was general public. There was statistically significance in the view count of the videos according to the primary and secondary classifications, the account used for uploading, and target subject of the videos. Conclusion: Dentists and their associates should recognize the importance of YouTube platform and try to monitor and intervene the dentistry-related contents, considering its huge impact on the general public.