• BMB Reports
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• v.54 no.1
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• pp.59-69
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• 2021

The ability to read, write, and edit genomic information in living organisms can have a profound impact on research, health, economic, and environmental issues. The CRISPR/Cas system, recently discovered as an adaptive immune system in prokaryotes, has revolutionized the ease and throughput of genome editing in mammalian cells and has proved itself indispensable to the engineering of immune cells and identification of novel immune mechanisms. In this review, we summarize the CRISPR/Cas9 system and the history of its discovery and optimization. We then focus on engineering T cells and other types of immune cells, with emphasis on therapeutic applications. Last, we describe the different modifications of Cas9 and their recent applications in the genome-wide screening of immune cells.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.5
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• pp.843-850
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• 2006

The standardization on RDD and REL has been developed by MPEG. REL is a Right Expression Language and RDD is term dictionary which develop active application of REL. However, since REL documents could be oかy edited by user understanding MPEG-21's framework, much easier editing system is required. As well, REL Consumption System to process and analyze REL documents, the RDD interoperability system to interoperate REL and RDD are needed. In this paper, REL Editing System and REL Consumption System, RDD Web Services System were designed and implemented, REL Editing System to make REL document, REL Consumption System to process and analyze edited documents, RDD Web Services System to process rights inquiry base on the Web Services.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.903-920
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• 2020

Since its first demonstration as a practical genome editing tool in the early 2010s, the use of clustered regularly interspaced short palindromic repeat (CRISPR) along with the endonuclease Cas9 (CRISPR/Cas9) has become an essential choice for generating targeted mutations. Due to its relative simplicity and cost-effectiveness compared to other molecular scissors, i.e., zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN), the CRISPR/Cas9 system has been shown to have a massive influence on genetic studies regardless of the biological kingdom. Although the system is in the process of being established, numerous protocols have already been released for the system and there have been various topics of CRISPR related papers published each year in ever-increasing manner. Here, we will briefly introduce CRISPR/Cas9 system and discuss the variants of the CRISPR system. Also, their applications to crop improvement will be dealt with mainly ornamental crops among horticultural crops other than Arabidopsis as a model plant. Finally, some issues on the barriers restraining the use of CRISPR system on floricultural crops, the prospect of CRISPR system as a DNA-free genome editing tool with efficient facilitators and finally, the future perspectives on the CRISPR system will be described.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.10
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• pp.5130-5143
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• 2019

Football (soccer) is one of the most popular sports in the world. A huge number of people watch live football matches by TV or Internet. A football match takes 90 minutes, but viewers may only want to watch a few highlights to save their time. As far as we know, there is no such a product that can be put into use to achieve intelligent highlight extraction from live football matches. In this paper, we propose an intelligent editing system for live football matches. Our system can automatically extract a series of highlights, such as goal, shoot, corner kick, red yellow card and the appearance of star players, from the live stream of a football match. Our system has been integrated into live streaming platforms during the 2018 FIFA World Cup and performed fairly well.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.7 s.112
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• pp.704-711
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• 2006

Vibration test on MAST(multi axial simulation table) system has several advantage over one-axial vibration test that could simulate 6-DOF, 3-axial translation and 3-axial moment, at the same time. Since field vibration motion can be fully represented with 6-DOF, multi-axial vibration test on vehicle component is widely conducted in technical leading companies to make sure its fatigue performance in vibration environment. On the way to fulfill the process, editing technique of obtained field data is key issue to success a reliable vibration testing with MAST system. Since the original signals are not only too large to fulfill it directly, but all of the measured data is not guarantee its convergency on generating its driving files, editing technique of the original signals are highly required to make some events that should meet the equal fatigue damage on the target component In this paper, key technique on editing a field data feasible for MAST system is described based on energy method in vibration fatigue. To explain its technique explicitly, author first introduced a process on field data acquisition of two vehicle component and then, representing events are produced to keep up with the editing strategy about a energy method. In the final chapter, a time information regarding a vibration test on MAST system is derived from the energy data which is critical information to perform a vibration test.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.764-769
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• 2006

Vibration test on MAST(multi axial simulation table) system has several advantage over one-axial vibration test that could simulate 6-DOF, 3-axial translation and 3-axial moment, at the same time. Since field vibration motion can be fully represented with 6-DOF, multi-axial vibration test on vehicle component is widely conducted in technical leading companies to make sure its fatigue performance in vibration environment. On the way to fulfill the process, editing technique of obtained field data is key issue to success a reliable vibration testing with MAST system. Since the original signals are not only too large to fulfill it directly, but all of the measured data is not guarantee its convergency on generating its driving files, editing technique of the original signals are highly required to make some events that should meet the equal fatigue damage on the target component In this paper, key technique on editing a field data feasible for MAST system is described based on energy method in vibration fatigue. To explain its technique explicitly, author first introduced a process on field data acquisition of two vehicle component and then, representing events are produced to keep up with the editing strategy about a energy method. In the final chapter, a time information regarding a vibration test on MAST system is derived from the energy data which is critical information to perform a vibration test.

• Proceedings of the Korea Contents Association Conference
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• 2008.05a
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• pp.147-151
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• 2008

Since the first HDV(High Definition Video) camcoder, HDR-FX1 with 1080i resolution, had been launched at 2004, the image capture and record with HD resolution have been possible by the various types of camcoder and recorder which can support the HDV format. At a present day, a non-linear editing system, which is composed of only one computer, can capture, edit and export the image with HDV format. The HDV format has the same class of resolution with HD and the same data transfer bit rate of DV(Digital Video). Additionally, the image with HDV format can be recorded in 6mm DV cassette tape. It is possible because the HDV format uses the concept of Long GOP(Gorup of pictures) method for its compression. In this paper, the characteristic feature of HDV format, the essential factors in editing with HDV format and the concept of Long GOP method for its compression have been studied for the way of effective HDV editing.

• Molecules and Cells
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• v.39 no.9
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• pp.687-691
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• 2016

Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the convenient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.

• BMB Reports
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• v.50 no.5
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• pp.247-256
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• 2017

CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research.

• Journal of Korea Multimedia Society
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• v.19 no.8
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• pp.1619-1625
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• 2016

Recently, as the public interests about the 3D printing technology are increased, various kinds of 3D printers are being released. But, they are limited to use because they cannot fabricate an object which is larger than the printer's printing volume. To relieve this problem, we propose an interactive 3D mesh editing system for 3D printing the object that is larger than the printing volume. The proposed 3D editing system divides the input 3D mesh using the user's line drawings defining cutting planes and it attaches various connectors. The output meshes are guaranteed to fabricate without post-processing. The printed parts can be assembled using the connectors. Our proposed system has an advantage that it can be used easily by non-professional 3D printer users.