• 한국동물위생학회지
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• 제36권1호
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• pp.37-44
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• 2013

The ecologically and economically important honeybee species are susceptible to infections by various pathogens. This study was investigated to detect infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province by PCR in 2010~2011. A total of 11 infectious pathogens, including 6 viruses, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptoms of sudden collapse, depopulation, or paralysis. The infectious pathogens and infection rates among 24 honeybee colonies detected were as follows: sacbrood virus (66.7%), deformed wing virus (4.2%), black queen cell virus (12.5%), Kashmir bee virus (29.2%), American foulbrood (41.7%), European foulbrood (12.5%), stonebrood (45.8%), chalkbrood (4.2%), and Nosema (33.3%), respectively. Since the coinfection rates of multiple pathogens were detected high in honeybee colonies reared in eastern Gyeongbuk province, large-scale investigation and appropriate control programs need to be established in this region.

• 한국동물위생학회지
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• 제33권2호
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• pp.129-134
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• 2010

Canine brucellosis is a contagious disease of the reproductive tract that cause mainly abortion and infertility in dog. A serological and bacteriological survey was conducted for breeding kennels which were suffered from frequent outbreak of canine brucellosis in Gyeongbuk province in 2009. Among 138 samples, 45 serum samples were sero-positive. Brucella canis was isolated from 30 blood samples of the seropositive cases, and from 2 samples of 62 sero-negatives. The biochemical properties of 32 isolates were characterized with no production of H2S, no fermentation of carbohydrates, hydrolyzation of urea, and development of thionin dye medium. At amplification of BCSP and 16S-rRNA gene using PCR, 711bp and 905bp DNA fragments were detected in agarose. Three tandem repeat pattern was shown in genotyping by Multi-locus VNTR assay (MLVA).

• 한국동물위생학회지
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• 제36권4호
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• pp.243-248
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• 2013

Q fever is a rickettsial infection caused by Coxiella burnetii that is transmitted from animals to humans. Modes of transmission to humans include inhalation, tick bites and ingestion of unpasteurized milk or dairy products. This survey was aimed at monitoring the seroprevalence of C. burnetii in bulk-tank milk (BTM) in Gyeongbuk province. In addition, the seroprevalence of C. burnetii was investigated at the herd level of dairy cattle in eastern Gyeongbuk province in which many dairy cattle are reared. Among 324 BTM samples collected from 20 country areas, 175 (54%) BTM samples from 15 (75%) country areas were positive for C. burnetii by ELISA. By regions, the seroprevalence of BTM samples in eastern, central, western and northern areas of Gyeongbuk province were 62.7%, 48.4%, 45.1%, and 41.4%, respectively. When analyzed in the dairy cattle reared in the eastern area where high seroprevalence occurred in BTM samples, 119 (24.2%) out of 492 dairy cattle were positive for C. burnetii. Seroprevalence of C. burnetii in dairy cattle was increased with daily milk yield of farm (P<0.05) and age (P<0.001). Since seroprevalence of C. burnetii is relatively high in both BTM samples and dairy cattle reared in Gyeongbuk province, further studies on the high risk farms and herds are needed to evaluate infection status and appropriate control programs in this region.

• 한국동물위생학회지
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• 제32권2호
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• pp.131-137
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• 2009

This study was conducted to determine the prevalence of antibodies to Toxoplasma gondii (TG) in cattle and pigs reared in eastern areas of Gyeongbuk province by ELISA. Among 368 sera collected from 119 cattle farms, 76 (20.7%) sera from 34 (28.6%) farms had antibodies to TG. Fifty (27.2%) out of 184 cattle in Uljin-gun and 26 (14.1%) out of 184 cattle in Yeongdeok-gun were positive. Pyeonghae (50.0%) in Uljin-gun and Dalsan (33.3%) in Yeongdeok-gun had the highest TG antibodies in cattle compared to other areas. Prevalence of TG antibodies in cattle was increased with age. Among 368 sera collected from 43 pig farms, 62 (16.8%) sera from 16 (37.2%) farms had antibodies to TG. Forty (21.7%) out of 184 pigs in Uljin-gun and 22 (12.0%) out of 184 pigs in Yeongdeok-gun were positive. Uljin and Puk (40.0%) in Uljin-gun and Yeonghae (33.3%) in Yeongdeok-gun had the highest TG antibodies in pigs compared to other areas. Prevalence of TG antibodies in sows was higher than that in fattening pigs. Seasonally, prevalence of TG antibodies in pigs was highest in summer (23.4%) and lowest in winter (12.5%). Based on these observations, data indicate that infection by the protozoan parasite TG is widely prevalent in cattle and pigs reared in eastern areas of Gyeongbuk province.

• 한국동물위생학회지
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• 제34권3호
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• pp.251-258
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• 2011

This study was performed to determine the prevalence of Theileria sergenti (T. sergenti) in cattle reared in eastern areas of Gyeongbuk province by PCR. Among 443 samples collected from 42 cattle farms, 96 (21.7%) samples from 13 (31.0%) farms were positive for T. sergenti. By regions, 87 (26.6%) out of 327 cattle in Gyeongju, 8 (9.5%) out of 84 cattle in Pohang, and 1 (3.1%) out of 32 cattle in Ulleung were positive for T. sergenti. T. sergenti infection in dairy cattle (27.9%) was significantly higher than that in Korean cattle (9.4%, P<0.0001). Accordingly, Korean cattle were more resistant to T. sergenti infection. Prevalence of T. sergenti in cattle was increased with age (P<0.0001). The infection rate in cows (23.3%) was significantly higher than that in bulls (5.0%, P<0.01). Seasonally, prevalence of T. sergenti in cattle was highest in autumn (32.7%, P<0.01). Prevalence of T. sergenti in grazing cattle (66.7%) was significantly higher than that in non-grazing cattle (15.8%, P<0.0001). Since prevalence of T. sergenti infection is high in cattle reared in eastern areas of Gyeongbuk province, survey on other hemoparasites and appropriate control programs need to be established in this region.

• 한국동물위생학회지
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• 제36권4호
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• pp.249-254
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• 2013

This study was done to determine the seroprevalence of Coxiella burnetii in cattle with reproductive disorders reared in eastern Gyeongbuk province. A total of 146 cattle blood samples from 83 farms in eastern Gyeongbuk province were collected in 2011~2012. Among them, 17 (11.6%) samples from 11 (13.3%) farms were seropositive for C. burnetii by ELISA. In dairy cattle with mastitis, 15 (38.5%) milk samples from 9 (42.9%) farms were seropositive among 39 milk samples from 21 farms. Among the seropositive farms, one farm with high seroprevalence was selected to assess the seroprevalence of C. burnetii in the bulk-tank milk (BTM) and blood samples of dairy cattle. The BTM sample from the farm was seropositive to C. burnetii and 6 (15%) out of 40 dairy cattle were seropositive. In cattle with reproductive disorders including infertility, abortion and stillbirth, 2 (1.9%) samples from 2 (3.2%) farms were positive among 107 samples collected from 62 farms. Among 2 positive samples, one of them is dariy cattle (100%) and the other is Korean cattle (0.9%) (P<0.001). This suggests that Korean cattle was relatively resistant to C. burnetii. Based on the data obtained, we identified association between C. burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. Since seroprevalence of C. burnetii is relatively high in dairy cattle, further intensive studies on C. burnetii among the high risk milk farms and dairy cattle herds are needed to evaluate infection status.

• 한국동물위생학회지
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• 제37권4호
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• pp.241-246
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• 2014

Q fever is a zoonotic disease caused by the obligate intracellular bacterium Coxiella (C.) burnetii and affects wild and domestic animals worldwide. The aim of this study was to evaluate the seroprevalence of C. burnetii in native Korean goat (Capra hircus coreanae) in Gyeongbuk province, Korea, using ELISA. A total of 256 goat blood samples from 56 farms in Gyeongbuk province were collected between May 2012 and March 2013. Among them, 22 (8.6%) samples from 10 (17.9%) farms were seropositive for C. burnetii by ELISA. According to regional analysis, the seroprevalences among goat farms in eastern, western, southern, and northern areas of Gyeongbuk province were 0%, 18.2%, 36.8%, and 6.3%, respectively, showing the highest seroprevalence in the southern region. Among 22 counties in Gyeongbuk province, 10 (45.5%) counties had one or more farms positive to C. burnetii antibody. Accordingly, the seroprevalence of C. burnetii in high-risk humans and animals are constantly demanded by regional investigation.

• 한국응용곤충학회지
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• 제46권1호
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• pp.27-35
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• 2007

영남지방의 지역 및 기후지대별 담배거세미나방과 파밤나방의 발생소장을 각각의 성페로몬을 이용하여 조사하였다. 경람지역에서 담배거세미나방은 3월 중하순부터 11월 중하순까지 발생하였다. 경북지역에서 담배거세미나방은 4월 중하순부터 11월 상중순까지 발생하였으나, 울진에선 5월 하순부터 10월 하순까지 발생하였다. 기후지대별 담배거세미나방의 발생최성기는 남부해안지대에서 9월 중순, 내륙지대 및 분지지대에서 8월 하순, 내륙산간지대에서 8월 중순, 그리고 동해안중부지대에서 9월 상순이었다. 담배거세미나방의 지역별 발생비율은 경남의 경우 진주에서 20.0%로 가장 높았으며, 창녕에서 8.6%로 가장 낮았고, 경북의 경우 성주에서 3.3%로 가장 높았으며, 울진에서 0.8%로 가장 낮았다. 기후지대별 발생비율은 내륙지대에서 43.7%로 가장 높았으며, 다음은 남부해안지대에서 36.3%, 분지지대에서 8.4%, 내륙산간지대에서 7.0%이었고, 동해안중부지대에서 4.6%로 가장 낮았다. 한편, 파밤나방은 경북의 동해안중부지대를 제외하곤 3월 상순부터 11월 중순까지 발생하였다. 포항, 영덕 및 울진의 동해안중부지대에서 파밤나방은 대체로 4월 중순부터 11월 중순까지 발생하였다. 기후지 대별 파밤나방의 발생최성기는 내륙지대, 분지지대 및 내륙산간지대에서 8월 상순, 남부해안지대 및 동해안중부지대에서 8월 하순이었다. 파밤나방의 지역별 발생비율은 경남의 경우 창녕에서 1.6%로 가장 높았으며, 부산에서 0.4%로 가장 낮았고, 경북의 경우 성주에서 37.2%로 가장 높았으며, 울진에서 2.7%로 가장 낮았다. 기후지대별 발생비율은 분지지대에서 약 54.3%로 가장 많았으며, 다음은 내륙산간지대에서 29.9%, 동해안중부지대에서 9.3%, 내륙지대에서 3.9%이었고, 남부해안지대에서 2.6%로 가장 낮았다. 따라서 담배거세미나방은 경남지방에서, 파밤나방은 경북지방에서 다발생 되었고, 기후지대별 담배거세미나방의 발생량은 경남의 내륙지대, 파밤나방의 발생량은 경북의 분지지대에서 가장 많았다. 영남지방에서 담배거세미나방의 발생량은 파밤나방보다 2.4배 많았으나, 최초 발생시기와 발생최성기는 담배거세 미나방보다 파밤나방이 약간 빨랐다.

• 한국작물학회지
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• 제50권spc1호
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• pp.94-98
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• 2005

경북 지역 농업기후 지대를 미질 및 식미치를 근거로 하여 다시 세분화된 지대를 설정하고자 2002년부터 2004년까지 3년간 경북 전 지역에서 가장 많이 재배되고 있는 품종 중 중만생종인 일품벼, 주남벼, 남평벼, 중생종인 화영벼, 조생종인 오대벼를 재배하고 있는 논 513개소의 쌀을 수집하여 분석 조사한 결과를 요약하면 다음과 같다. 1. 지대별 식미치의 평균은 동해안 남부지대가 67.6로 다른 지대에 비해 약간 낮았으며, 완전미 비율은 동해안 남부지대 및 동해안 중부지대가 각각 $87.3\%,\;88.2\%$로 낮았으며 변이계수도 각각 $8.2\~8.3\%$로 높았다. 단백질 함량은 동해안 중부지대가 $8.0\%$로 다른 지대에 비해 약간 높았고, 변이계수도 $8.8\%$ 높았다. 2. 특정지역을 세분하여 쌀 품질을 조사해보면 전체 평균의 변이계수보다 높게 나타나는 지역과 낮게 나타나는 지역으로 뚜렷하게 구분된다. 변이 계수가 크다는 것은 특정 지역 내에서도 쌀 품질의 세분화된 지역간 차이가 심하므로 고품질 쌀 생산을 위해서는 세분화된 벼 재배 구역 설정하여 적지적작의 정밀농업이 필요하였다. 3. 식미치를 근거로 세분화된 지대의 쌀 품질 요인에 대한 연차 간 변이계수는 기존 농업기후지대의 변이계수보다 상당히 낮아져, 세분화된 지대별 고품질 쌀 생산을 위한 특정 지역의 정밀 기술 개발 및 보급의 기초 자료로 활용할 수 있을 것으로 사료되었다.

• 한국동물위생학회지
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• 제39권3호
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• pp.205-210
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• 2016

BSE (bovine spongiform encephalopathy)-like symptoms salivation, gait disorder, and the downer syndrome occurred to 8 heads of cattle at six cattle farms in Gyeonju, east eastern Gyeongsang province in 2013. Eight brain tissue samples using a vibration saw (Multimaster$^{(R)}$ FEIN, Germany) were collected referring to "The brain removal technique of transmisible spongiform encephalopathy sampling DVD" by Animal And Plant Quarantine Agency. Brain removal techinque using a vibration saw on the thick, solid frontal bone of cattle is more convenient for incision and removal than an ax for BSE testing.