• 제목/요약/키워드: E-Science

검색결과 25,255건 처리시간 0.049초

Protective Immunity of Pichia pastoris-Expressed Recombinant Envelope Protein of Japanese Encephalitis Virus

  • Kwon, Woo-Taeg;Lee, Woo-Sik;Park, Pyo-Jam;Park, Tae-Kyu;Kang, Hyun
    • Journal of Microbiology and Biotechnology
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    • 제22권11호
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    • pp.1580-1587
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    • 2012
  • Japanese encephalitis virus (JEV) envelope (E) protein holds great promise for use in the development of a recombinant vaccine. Purified recombinant E (rE) protein may be useful for numerous clinical applications; however, there are limitations in using the Escherichia coli expression system for producing high-quality rE protein. Therefore, in this study, the yeast expression system was used to generate the rE protein. For protein production using the yeast system, the full-length JEV E gene was cloned into Pichia pastoris. SDS-PAGE and immunoblotting analysis demonstrated that the rE protein had a molecular mass of 58 kDa and was glycosylated. The predicted size of the mature unmodified E protein is 53 kDa, suggesting that post-translational modifications resulted in the higher molecular mass. The rE protein was purified to greater than 95% purity using combined ammonium sulfate precipitation and a SP-Sepharose Fast Flow column. This purified rE protein was evaluated for immunogenicity and protective efficacy in mice. The survival rates of mice immunized with the rE protein were significantly increased over that of Hyphantria cunea nuclear polyhedrosis virus E protein (HcE). Our results indicate that the rE protein expressed in the P. pastoris expression system holds great promise for use in the development of a subunit vaccine against JEV.

Growth curve estimates for wither height, hip height, and body length of Hanwoo steers (Bos taurus coreanae)

  • Park, Hu-Rak;Eum, Seung-Hoon;Roh, Seung-Hee;Sun, Du-Won;Seo, Jakyeom;Cho, Seong-Keun;Lee, Jung-Gyu;Kim, Byeong-Woo
    • 농업과학연구
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    • 제44권3호
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    • pp.384-391
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    • 2017
  • Growth curves in Hanwoo steers were estimated by Gompertz, Von Bertalanffy, Logistic, and Brody nonlinear models using growth data collected by the Hanwoo Improvement Center from a total of 6,973 Hanwoo (Bos taurus coreanae) steers 6 to 24 months old that were born between 1996 and 2015. The data included three parameters: A, mature size of body measurement; b, growth ratio; and, k, intrinsic growth rate. Nonlinear regression equations for wither height according to Gompertz, Von Bertalanffy, Logistic, and Brody models were $Y_t=144.7e^{-0.5869e^{-0.00301t}}$, $Y_t=145.3(1-0.1816e^{-0.00284t})^3$, $Y_t=143.1(1+0.7356e^{-0.00352t})^{-1}$, and $Y_t=146.8(1+0.4700e^{-0.00249t})^1$, respectively, while those for hip height were $Y_t=144.5e^{-0.5549e^{-0.00312t}}$, $Y_t=145.0(1-0.1724e^{-0.00295t})^3$, $Y_t=143.1(1+0.6863e^{-0.00360t})^{-1}$, and $Y_t=146.2(1+0.4501e^{-0.00263t})^1$, respectively. Equations for body length $Y_t=174.1e^{-0.8342e^{-0.00289t}}$, $Y_t=175.8(1-0.2500e^{-0.00265t})^3$, $Y_t=170.0(1+1.1548e^{-0.00363t})^{-1}$, and $Y_t=180.3(1+0.6077e^{-0.00215t})^1$, respectively, for the same models. Among the four models, the Brody model resulted in the lowest mean square error, with mean square errors of 31.79, 30.57, and 42.13, respectively, for wither height, hip height, and body length. Also, an estimated birth wither height, birth hip height, and birth body length (77.98, 80.57, and 70.97 cm, respectively) were lower in the Brody model than in other models. An inflection point was not observed during the growth phase of Hanwoo steer according to the growth curves calculated using Gompertz, Von Bertalanffy, and Logistic models. Based on the results, we concluded that the regression equation using the Brody model was the most appropriate among the four growth models. To obtain more accurate parameters, however, using data from a wider production period (from birth to shipping) would be required, and the development of a suitable model for body conformation traits would be needed.

Inhibition of Cervical Cancer Cell Growth by Gene Silencing of HPV16 E6 Induced by Short-interfering RNA

  • Park, Sang-Muk;Lee, Sun-Kyung;Kim, Yoon-Sik
    • 대한임상검사과학회지
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    • 제43권3호
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    • pp.89-97
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    • 2011
  • The Human Papilloma Virus (HPV) infection has been strongly associated with pathogenesis of uterine cervix carcinoma. HPV type 16, a causative agent of uterine cervix carcinoma, encodes the E6 and E7 oncogenes, expression of which is pivotal for malignant transformation and maintenance of malignant phenotypes. To develop a gene therapy for HPV-related carcinoma, We investigated the effect of E6 short-interfering RNA (E6 siRNA) on the expression of this oncogene and on the growth of HPV 16-related uterine cervix carcinoma cells. SiHa cells, a uterine cervix carcinoma cell line, which contain a single copy of HPV 16 integrated in the chromosome and express the E6 and E7 oncogenes. Before 24 hr of transfection, cells were seeded and transfected with control plasmid or E6 siRNA-expressing plasmid. The mRNA was analysed by reverse transcriptase polymerase chain reaction (RT-PCR). The cell growth rate was investigated by MTT method. The E6 mRNA level in SiHa cells was decreased in HPV 16 E6 siRNA-expression vector transfected cells and a decrease in the growth of these cells was also observed. From these results. it is evident that E6 siRNA played a role in suppression of growth of SiHa cells and has a fair chance as a candidate for gene specific therapy for HPV related uterine cervix carcinoma.

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Taxpayer Behavior in Using E-Vehicle in Indonesia

  • Evi Zubaidah;Achmad Nurmandi;Ulung Pribadi;Mega Hidyati
    • Asia pacific journal of information systems
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    • 제31권3호
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    • pp.378-391
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    • 2021
  • The low use of e-vehicles in Yogyakarta City and Pekanbaru City is an essential issue for local governments to maximize e-government policies in both regions. The purpose of this research is to analyze people's attitudes and factors influencing the community of e-Samsat (e-Vehicle tax) users in developing the UMEGA theory, namely technology and government trust. This is a quantitative research based on the philosophy of positivism, which is used to examine populations and individual samples. Data were collected using research instruments and quantitatively analyzed. Furthermore, the hypothesis of the obtained data was tested using SEM-PLS. The results showed that social influence does not affect attitudes of e-Samsat (e-Vehicle tax) users. Furthermore, the study showed that the development of umega theory by adding variables of trust in technology and government affects people's behavior in using e-Samsat services.

과학기술특성화대학(KAIST)을 중심으로한 R&E 집중연구가 과학고등학교 학생에게 미치는 영향 (Effects of Science high school students' Competency through Intensive Research and Education Program in KAIST)

  • 최진수;김영민;이영주
    • 한국과학교육학회지
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    • 제39권6호
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    • pp.729-738
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    • 2019
  • R&E 프로그램은 연구 중심의 자기주도적 학습을 통하여 과학영재들이 과학적 탐구능력과 창의적인 문제해결 능력을 신장시킬 수 있도록 하며, 관심 분야 전문가와의 긴밀한 만남과 과학탐구 경험을 통해 과학자로서의 연구 태도와 품성 및 자질을 함양하고, 창의성을 계발하도록 하는 것에 목적이 있다. R&E 프로그램의 교육적 효과로 미래과학기술 인력의 질적 향상뿐만 아니라 과학자들의 교육참여를 유도함으로써 교육 인력의 저변 확대를 통한 과학영재 교육의 질적 내실화를 도모할 수 있다. 본 연구에서는 R&E 프로그램이 미래 과학기술인력으로써 필요한 창의인재역량과 첨단과학기술 분야 진로 지향성에 미치는 영향을 살펴보기 위하여 KAIST 과학영재교육연구원의 R&E 프로그램에 참여한 D과학고등학교 1학년 학생 62명을 대상으로 집중연구 사전, 사후에 걸쳐 창의인재역량과 과학진로지향의 변화를 설문을 통해 분석하였다. 그 결과 학생들은 R&E 프로그램 참여 후 창의인재역량 중 인지적, 정의적 특성이 유의미하게 증가하였고, 사회적 특성에서는 유의한 변화가 없었으나 세부영역에서는 유의미한 증가를 확인하였다. 또한, 과학진로지향과 관련하여 과학학습, 과학진로에 대한 선호도, 과학진로에 대한 가치 인식 및 과학진로 정보의 필요성 항목에서 유의미한 변화가 없는 것으로 나타났다. 본 연구를 통해 R&E 프로그램의 교육적 효과를 보여줌으로써 대상과 지도목표에 따른 R&E 프로그램 모델의 개선방안을 제안하고자 하며, 나아가 연구역량 교육 및 과학진로진학을 지도하는데 기초 자료로 활용할 수 있을 것이다.

e-Science 국내외 기술 동향

  • 황순욱;남덕윤;김법균;염헌영
    • 정보처리학회지
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    • 제15권2호
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    • pp.43-57
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    • 2008
  • IT기술의 눈부신 발전으로 인해 과학적 연구(scientific research)는 더 이상 한 개인 연구자의 실험실내에서 수행되는 것이 아니라 학제, 실험실, 연구기관, 심지어는 국가를 포괄한 연구자 커뮤니티의 협업 형태로 수행되고 있다. e-Science란 이처럼 IT 기반으로 과학, 공학에 있어서 새로운 문제들을 풀기 위한 자원과 연구자들의 글로벌 협업을 말한다. 우리나라도 지난 2005년부터 국가적 차원에서 e-Science 사업을 진행해 오고 있다. 유럽도 지난 2004년부터 소위 EGEE (Enabling Grid for E-SciencE)라는 e-Science 프로젝트를 전 유럽차원에서 진행해 오고 있다. 본 논문에서는 그동안 진행해온 한국과 유럽의 e-Science 환경 구축에 있어서 핵심적인 역할을 하고 있는 e-Science 기반 기술에 대해서 소개를 하고자 한다.

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Double Mutations in eIF4E and eIFiso4E Confer Recessive Resistance to Chilli Veinal Mottle Virus in Pepper

  • Hwang, JeeNa;Li, Jinjie;Liu, Wing-Yee;An, Song-Ji;Cho, Hwajin;Her, Nam Han;Yeam, Inhwa;Kim, Dosun;Kang, Byoung-Cheorl
    • Molecules and Cells
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    • 제27권3호
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    • pp.329-336
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    • 2009
  • To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an elF4E mutation ($pvr1^2$) and C. annuum 'Perennial' containing an elFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All $F_1$ plants showed resistance, and $F_2$ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five $F_2$ and 329 $F_3$ plants of 17 families were genotyped with $pvr1^2$ and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both $pvr1^2$ and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in elF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of $F_2$ plants revealed that all plants containing homozygous genotypes of both $pvr1^2$ and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of elF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

Alteration of Lipid Metabolism by Ginseng Supplements With Different Levels of Vitamin E in High Cholesterol-Fed Rats

  • Do, Kyung-Min;Park, Yong-Bok;Bok, Song-Hae;Lee, Mi-Kyung;Jeong, Tae-Sook;Choi, Myung-Sook
    • Preventive Nutrition and Food Science
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    • 제6권1호
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    • pp.66-72
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    • 2001
  • Ginseng may have antioxidant and pharmacologic effects similar to those of vitamin E. The interactive effect of ginseng and vitamin E was studied with respect to cholesterol metabolism and the antioxidant status. A ginseng supplement (0.1%, wt/wt) with comparable levels of vitamin E was provided with a high-cholesterol (1%, wt/wt) diet to rats for 5 weeks. The amount of vitamin E included in the ginseng-free and ginseng diets was either a low (low-E) or a normal (normal-E) level. The ginseng supplements significantly (p<0.05) altered the concentrations of plasma triglycerides in both the low-vitamin E group and normal-vitamin E group compared to the each ginseng-free group. The hepatic triglyceride and cholesterol content were not significantly (p>0.05) different between groups regardless of the vitamin E level in the diet. The hepatic HMG-CoA reductase activity was significantly (p<0.05) lowered by the ginseng supplement in both the low-vitamin E and the normal-vitamin E groups compared to the ginseng-free group. The HMG-CoA reductase activity was also significantly (p<0.05) lowered with in increase of the dietary vitamin E in the ginseng-free group. The excretion of fecal neutral sterol was significantly (p<0.05) lower in the normal-E ginseng group than th low-E ginseng-free group. Neither dietary ginseng nor vitamin E significantly changed the hepatic antioxidant enzymes activity. This data indicates that ginseng supplements lower the concentration of plasma triglyceride and hepatic HMG-CoA reductase activity regardless of eh dietary vitamin E level. This information may contribute to understanding the interactive effect of ginseng and vitamin E on cholesterol biosynthesis in high cholesterol-fed rats.

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Virtual Screening을 위한 e-Science 프로젝트 동향 (The Trend of e-Science Projects for Virtual Screening)

  • 김남규;안선일;이세훈;이준학;황순욱
    • 한국콘텐츠학회:학술대회논문집
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    • 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
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    • pp.569-573
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    • 2007
  • e-Science는 이전의 연구수행방법을 최신의 정보통신기술을 적용하여 연구생산성을 향상시키는 새로운 패러다임이다. 이러한 e-Science를 적용하는 연구 분야 중에서도 바이오 분야는 바이오인포매틱스 학문이 만들어질 정도로 정보통신 기술을 많이 사용하는 분야이다. 이 바이오 분야에 적용되는 IT기반의 기술 중에서 대용량의 컴퓨팅 자원을 바탕으로 한 e-Science가 적용되는 분야가 신약 개발 과정 중 하나인 Virtual Screening이다. 이 Virtual Screening을 수행하는 e-Science 프로젝트 중 WISDOM과 Avian Flu에 대해 알아보고 국내에서 진행되고 있는 프로젝트의 진행 상황을 살펴본다. 그리고 앞으로 국내에서 KISTI와 전남대가 계획하고 있는 Virtual Screening에 대한 공동 연구에 대한 향후 방향을 설정해본다.

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A Review of Structural Testing Methods for ASIC based AI Accelerators

  • Umair, Saeed;Irfan Ali, Tunio;Majid, Hussain;Fayaz Ahmed, Memon;Ayaz Ahmed, Hoshu;Ghulam, Hussain
    • International Journal of Computer Science & Network Security
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    • 제23권1호
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    • pp.103-111
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    • 2023
  • Implementing conventional DFT solution for arrays of DNN accelerators having large number of processing elements (PEs), without considering architectural characteristics of PEs may incur overwhelming test overheads. Recent DFT based techniques have utilized the homogeneity and dataflow of arrays at PE-level and Core-level for obtaining reduction in; test pattern volume, test time, test power and ATPG runtime. This paper reviews these contemporary test solutions for ASIC based DNN accelerators. Mainly, the proposed test architectures, pattern application method with their objectives are reviewed. It is observed that exploitation of architectural characteristic such as homogeneity and dataflow of PEs/ arrays results in reduced test overheads.