• Nuclear Medicine and Molecular Imaging
• /
• v.41 no.3
• /
• pp.226-233
• /
• 2007

Purpose: Dual reporter gene imaging has several advantages for more sophisticated molecular imaging studies such as gene therapy monitoring. Herein, we have constructed hepatoma cell line expressing dual reporter genes of sodium iodide symporter (NIS) and enhanced green fluorescence protein (EGFP), and the functionalities of the genes were evaluated in vivo by nuclear and optical imaging. Materials and Methods: A pRetro-PN vector was constructed after separating NIS gene from pcDNA-NIS. RSV-EGFP-WPRE fragment separated from pLNRGW was cloned into pRetro-PN vector. The final vector expressing dual reporter genes was named pRetro-PNRGW. A human hepatoma (HepG2) cells were transfected by the retrovirus containing NIS and EGFP gene (HepG2-NE). Expression of NIS gene was confirmed by RT-PCR, radioiodine uptake and efflux studies. Expression of EGFP was confirmed by RT-PCR and fluorescence microscope. The HepG2 and HepG2-NE cells were implanted in shoulder and hindlimb of nude mice, then fluorescence image, gamma camera image and I-124 microPET image were undertaken. Results: The HepG2-NE cell was successfully constructed. RT-PCR showed NIS and EGFP mRNA expression. About 50% of cells showed fluorescence. The iodine uptake of NIS-expressed cells was about 9 times higher than control. In efflux study, $T_{1/2}$ of HepG2-NE cells was 9 min. HepG2-NE xenograft showed high signal-to-background fluorescent spots and higher iodine-uptake compared to those of HepG2 xenograft. Conclusion: A hepatoma cell line expressing NIS and EGFP dual reporter genes was successfully constructed and could be used as a potential either by therapeutic gene or imaging reporter gene.

• Journal of IKEEE
• /
• v.15 no.2
• /
• pp.122-128
• /
• 2011

Although conventional analog linescan cameras are used widely, high-speed, high-resolution Cameralink standard will lead the area of frame grabber industry such as factory automation. In this paper, we are developing embedded frame grabber testbed without PC which will give an another solution to image processing applications. Therefore, we designed hardware schematics and programmed FPGA device with VHDL in order to interface Cameralink standard linescan CCD camera. In the future, our embedded on-chip controller could be applied to various image processing systems such as medical imaging, especially optical coherence tomography, machine vision and industrial electronics.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.10 no.8
• /
• pp.867-874
• /
• 2015

Hoist have been installed at wharfs or seashore in the center of fishing village fraternities. A Hoist is used in harbor loading and unloading fishing gear or seafoods and is a device to refloat fishing boats into a breakwater or land in case of typhoon or bad weather. In this paper, we propose image perception and moving objects tracking system that detects boat's damage, theft and trespassing occurred at the wharf. This system detects objects' motion in real time by using the motion templet and tracks to concentrate on a moving object(person, boat, etc.) by using a PTZ camera.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 1997.04a
• /
• pp.61-67
• /
• 1997

The electronic speckle pattern interferometer (ESPI) has been applied to many technical problems such as deformation and displacement measurement, strain visualization and surface roughness monitoring. In this study, we used an ESPI system based on the dual beam speckle interferometer method in order to measure in-plane displacement and vibration mode using the ESIP technique. This research was carried out for the purpose of applying the vibration analysis method employing Electro-Optic holographic interference technique to the vibration analysis of uniform rectangular cantilevers plate(SS400,STS304) with cantilevers span to breadth ratio of 150 by 75. And thickness of 1mm and 0.8mm respectively. We improved the ESPI technique in order to obtain the distribution of displacement component resolved in one direction through a CCD camera combined with an image processing system. To certify and to assess the accuracy in measuring by this ESPI, the results obtained with the speckle method and vibration mode analysis are to be compared with those results by Warbuton's Theoretical expression and vibration made in FEM analysis.

• Journal of the Korean Society of Combustion
• /
• v.3 no.2
• /
• pp.13-27
• /
• 1998

The optimization of frontal device including fuel nozzle and swirler is required to secure the mixing of fuel and air and the combustion stability leading the reduction of pollutant emissions and the increase of combustion efficiency in gas turbine combustor. The effects of injection nozzle and swirler on the flow field, spray characteristics and consequently the combustion stability, were experimentally investigated by measuring the velocity field, droplet sizes of fuel spray, lean combustion limit and the temperature field in the main combustion region. Flow fields and spray characteristics were measured with APV(Adaptive Phase Doppler Velocimetry) under atmospheric condition using kerosine fuel. Temperatures were measured by Pt-Pt13%Rh, R-type thermocouple which was 0.2mm thick. Spray and flame was visualized by ICCD(Intensified Charge Coupled Device) camera. It was found that the dual swirler resulted in the biggest recirculation zone with the highest reverse flow velocity at the central region, which lead the most stable combustion. The various combustion characteristics were observed as a function of the geometries of injector and swirler, that gave a tip for the better design of gas turbine combustor.

• Proceedings of the KSME Conference
• /
• 2004.04a
• /
• pp.1631-1636
• /
• 2004

The supersonic impinging jet has been extensively applied to rocket launching system, gas jet cutting control, gas turbine blade cooling, etc. In such applications, wall temperature of an object on which supersonic jet impinges is a very important factor to determine the performance and life of the device. However, wall temperature data of supersonic impinging jets are not enough to data. The present study describes an experimental work to measure the wall temperatures of a vertical flat plate on which supersonic, dual, coaxial jet impinges. An Infrared camera is employed to measure the wall temperature distribution on the impinging plate. The pressure ratio of the jet is varied to obtain the supersonic jets in the range of over-expanded to moderately under-expanded conditions at the exit of coaxial nozzle. The distance between the coaxial nozzle and the flat plate was also varied. The coaxial jet flows are visualized using a Shadow optical method. The results show that the wall temperature distribution of the impinging plate is strongly dependent on the jet pressure ratio and the distance between the nozzle and plate.

• Korean Journal of Applied Biomechanics
• /
• v.13 no.2
• /
• pp.115-122
• /
• 2003

The purposes of this study were to analyze the muscle activities and the characteristics of muscle recruting patterns of lower limb for crouching start technique using four elite splint. The EMG technique was used to record muscle activities of both right and left sides of retus femoris, vastus medialis, biceps femoris, and gastrocnemius. Eight surface electrodes were placed on the surface of the selected muscles and one ground electrode was also attached on the back of neck(C7). One video camera was also used to record the crouching start motion to define 6 events and 5 phases for further analysis. The raw EMG data were filtered with band pass filter(10-350) to remove artifacts and then low pass filtered(4Hz) to find the linear envelope which resemble muscle tension curve. This filter EMG data were normalized to MVIC for the purpose of comparison between right and left sides muscle. The results of this study were as follows. All four subjects showed that vastus medialis muscle activity was comparatively higher than those of retus femoris, biceps femoris, gastrocnemius. And left side of muscle activity was comparatively higher than right side of muscle activity. Therefore, when the subjects starting out crouching start position, we recognized that the muscle of lower limb showed interaction effect by each muscle.

• Journal of Industrial Technology
• /
• v.39 no.1
• /
• pp.33-39
• /
• 2019

This paper addresses effects of nanoscale structures on structural colors of micropatterned transparent substrate by light diffraction. Structural colors is widely investigated because they present colors without any chemical pigments. Typically structural colors is presented by diffraction of light on a micropatterned surface or by multiple interference of light on a surface containing a periodic or quasi-periodic nano-structures. In this paper, each structural colors induced by quasi-periodic nano-structures, periodic micro-structures, and nano/micro dual structures is measured in order to investigate effects of nanoscale and microscale structures on structural colors in the transparent substrate. Using pre-fabricated pattern mold and hot-embossing process, nanoscale and microscale structures are replicated on the transparent PMMA(Poly methyl methacrylate) substrate. Nanoscale and microscale pattern molds are prepared by anodic oxidation process of aluminum sheet and by reactive ion etching process of silicon wafer, respectively. Structural colors are captured by digital camera, and their optical transmittance spectrum are measured by UV/visible spectrometer. From experimental results, we found that nano-structures provide monotonic colors by multiple interference, and micro-structures induce iridescent colors by diffraction of light. Structural colors is permanent and unchangeable, thus it can be used in various application field such as security, color filter and so on.

• Progress in Medical Physics
• /
• v.20 no.4
• /
• pp.216-224
• /
• 2009

$^{131}I$ is a radiological isotope being used widely for treatment of cancer as emitting gamma-ray and it is also applied to estimate the function of thyroid for its accumulation in thyroid. However, $^{131}I$ is more difficult to quantitate comapred to $^{99m}Tc$, because $^{131}I$ has multiple energy gamma-ray emissions compared to $^{99m}Tc$ which is a mono energetic gamma-ray source. Especially, scattered ray and septal penetration resulted by high energy gamma ray have a bad influence upon nuclear medicine image. The purpose of this study was to estimate scatter components depending on the different source locations within a phantom using Monte Carlo simulation (GATE). The simulation results were validated by comparing with the results of real experiments. Dual-head gamma camera (ECAM, Chicago, Illinois Siemens) with high energy, general-purpose, and parallel hole collimators (hole radius: 0.17 cm, septal thickness: 0.2 cm, length: 5.08 cm) was used in this experiment. The NaI crystal is $44.5{\times}59.1\;cm$ in height and width and 0.95 cm in thickness. The diameter and height of PMMA phantom were 16 cm and 15 cm, respectively. The images were acquired at 5 different locations of $^{131}I$ point source within the phantom and the images of $^{99m}Tc$ were also acquired for comparison purpose with low energy source. The simulation results indicated that the scattering was influenced by the location of source within a phantom. The scattering effects showed the same tendency in both simulation and actual experiment, and the results showed that the simulation was very adequate for further studies. The results supported that the simulation techniques may be used to generalize the scattering effects as a function of a point source location within a phantom.

• Nuclear Engineering and Technology
• /
• v.56 no.7
• /
• pp.2799-2805
• /
• 2024

Energy-resolved neutron imaging is an effective way to investigate the internal structure and residual stress of materials. Different sample sizes have varying requirements for the detector's imaging field of view (FOV) and spatial resolution. Therefore, a dual-mode energy-resolved neutron imaging detector was developed, which mainly consisted of a neutron scintillator screen, a mirror, imaging lenses, and a time-stamping optical fast camera. This detector could operate in a large FOV mode or a high spatial resolution mode. To evaluate the performance of the detector, the neutron wavelength spectra and the multiple spatial resolution tests were conducted at CSNS. The results demonstrated that the detector accurately measured the neutron wavelength spectra selected by a bandwidth chopper. The best spatial resolution was about 20 ㎛ in high spatial resolution mode after event reconstruction, and a FOV of 45.0 mm × 45.0 mm was obtained in large FOV mode. The feasibility was validated to change the spatial resolution and FOV by replacing the scintillator screen and adjusting the lens magnification.