• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.4
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• pp.90-102
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• 2024

Haploid/double haploid plants developed from isolated microspores can significantly accelerate plant breeding. Haploid plants can naturally double their chromosomes to create a pure homozygous line of diploid plants. We present a method for producing embryos from isolated microspores of hot peppers (Capsicum annuumL.). We analyzed the polyploidization levels of the regenerated plants. The donor plants produced the optimal stage of microspores following short-term growth under low-intensity light, which resulted in high rates of embryogenesis and cotyledonary embryogenesis. To find an efficient culture method, liquid, doubled-layer, and 2-step cultures were tested. Liquid culture yielded the highest number of embryos, whereas the highest efficiency for cotyledonary embryogenesis was afforded by the doubled-layer culture. When normal cotyledonary embryos were transplanted onto a regeneration medium, they developed into complete plants. From these, 208 plants were tested via flow cytometric analysis, and 35.6% and 72.7% of the chromosomes from the Milyang-jare and LV2319 genotypes, respectively, were found to be spontaneous double haploids. These results are the same as those obtained on analyzing horticultural characteristics, including the size of leaves and the size and shape of fruits. The present study provides information on the practical application of isolated microspore culture of hot peppers, factors that affect embryogenesis, and methods for polyploidy testing.

• Geophysics and Geophysical Exploration
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• v.27 no.1
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• pp.51-56
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• 2024

In this paper, the vector gravity and gravity gradient tensor of an elliptical disk are derived. The vector gravity of an elliptical disk is defined by differentiating the gravitational potential due to the elliptical disk expressed by a double integral with respect to each axial direction. The vector gravity defined by the double integral is then transformed into a line integral of a closed curve along the elliptical disk boundary using the complex Green's theorem. Finally, vector gravity due to the elliptical disk is derived by 1D parametric numerical integration along the elliptical disk boundary. The xz, yz, zz components of the gravity gradient tensor due to the elliptical disk are obtained by differentiating the vector gravity with respect to vertical direction. The xx, yy, xy components are derived by differentiating the horizontal components of the vector gravity in the form of a double integral with respect to horizontal directions and then using the complex Green's theorem.

• Journal of Life Science
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• v.19 no.4
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• pp.449-456
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• 2009

Murine Neuro-2a (N2a) cells have been widely used for the investigation of neuronal differentiation, trophic interaction and neurotoxic effects of various compounds and their associated mechanisms. N2a cells have many genomic variations such as gains or losses in DNA copy number, similar to other neuroblastoma cells, and no systematic or high-resolution studies of their genome-wide chromosomal aberrations have been reported. Presently, we conducted a systematic genome-wide determination of chromosomal aberrations in N2a cells using a high-throughput, oligonucleotide array-based comparative genomic hybridization (oaCGH) technique. A hidden Markov Model was employed to assign each genomic oligonucleotide to a DNA copy number state: double loss, single loss, normal, gain, double gain and amplification. Unlike most neuroblastoma cells, Mycn amplification was not observed in N2a cells. In addition, these cells showed gain only in the neuron-derived neurotrophic factor (NF), while other neurotrophic factors such as glial line-derived NF and brain-derived NF presented normal copy numbers. Chromosomes 4, 8, 10, 11 and 15 displayed more than 1000 aberrational oligonucleotides, while chromosomes 3, 17, 18 and 19 displayed less than 20. The largest region of gain was located on chromosome 8 and its size was no less than 26.7 Mb (Chr8:8427841-35162415), while chromosome 4 had the longest region of single deletion, with a size of 15.1 Mb (Chr4:73265785-88374165).

• Journal of Korean Society of Forest Science
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• v.107 no.3
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• pp.266-277
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• 2018

This study was conducted in clear cutting stands. For the whole-tree yarding operation, a tower-yarder based on excavator with a radio-controlled double clamp carriage was applied to analyze yarding productivity and cost. The productivity and cost of yarding operation was $6.2m^3/SMH$(machine utilization rate, 80.4%) and $13,705won/m^3$, respectively. Although the maximum yarding distance was longer than the previous studies, the setting up time of the tower-yarder was decreased as used straw line. The application of the radio-controlled double clamp carriage improved productivity and reduced costs. Based on the fuel log and growing stock($137.0m^3/ha$), the minimum production($m^3$) and area(ha) were $558.3m^3$ and 4.1 ha for Pinus rigida stands and $433.7m^3$ and 3.2 ha for Quercus mongolica stands, respectively. Based on the this study, it will be possible to select an efficient yarding machine by accumulation of data on minimum production and area for many machines in various harvesting sites.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.214-217
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• 2011

A light pink double freesia (Freesia hybrida Hort.) 'Pink Ribbon' was developed from a cross of breeding a line, 'Pink Jewel' derived 'Michelle' and 'Rossini' and 'Purple Rain' followed by seedling and line selections at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. Growth and flowering characteristic tests were conducted from 2004 to 2007. 'Pink Ribbon' has double type flower with light pink color (RHS, RP66C). It has simultaneous flowering of many florets, good flower shape, upright leaves and stalks. The average cut flower yield of 'Purple Rain' observed in the greenhouse from 2005 to 2007 was 7.7 cuttings per plant. It has good production of cormlets, vigorous growth, too. 'Purple Rain' could be cultured under greenhouse conditions in Korea.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.8
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• pp.156-164
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• 2014

In a delay line type of a time-to-digital converter implemented in Field Programmable Gate Array, the timing accuracy decreases for a longer carry chain. In this paper, we propose a structure that has a multi-phase clock and a state machine to check metastability; this would reduce the required length of the carry chain with the same time resolution. To reduce the errors caused by the time difference in the four delay lines associated with a four-phase clock, the proposed TDC generates a single input pulse from four phase clocks and uses a single delay line. Moreover, the state machine is designed to find the phase clock that is used to generate the single input pulse and determine the metastable state without a synchronizer. With the measurement range of 1 ms, the measured resolution was 22 ps, and the non-linearity was 25 ps.

• Journal of Fashion Business
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• v.15 no.1
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• pp.80-91
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• 2011

The purpose of this study is to evaluate the differences of visual effects and visual images by variations in shoulder angle and shoulder width of power shoulder jacket. The stimuli are 5 samples: One control group, 2 variations of the angle of shoulder line and 2 variations of the width of shoulder line. The data has been obtained from 87 fashion design majors. The data has been analyzed by Factor Analysis, Anova, Scheffe's Test and the Correlation Analysis. The results of the study are as follows: The visual images by the angle and the width of shoulder line of power shoulder jacket are composed of 4 factors : attention, stiffness and softness, masculinity, and trendiness. Among these factors, attention factor is estimated by the most important factor. The visual effects by the angle and the width of shoulder line of power shoulder jacket are composed of 3 factors : whole silhouette, neck and arm figure, upper body figure. In these factors, the whole silhouette is estimated by the most important factor. The power shoulder jacket with extreme changes in shoulder angle has double images: it looks trendy and classy but also gives an awkward image. In case of changes in shoulder width, the visual evaluation shows big differences with the degree of expansion. The visual evaluation with variation in angle has stronger image and effect than that in width. The power shoulder jacket trend itself is also evaluated that variation in angle reflects the trend better than that in width.

• Journal of the Korea Fashion and Costume Design Association
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• v.20 no.3
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• pp.27-32
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• 2018

Hanbok is a our own history and tradition and is an icon of Korean history and culture. Today, the costumes are moving around the world fashion trend due to the development of mass media and internet at the same time. This is an important clue to marketing activities and can be used for predictive analysis. Although Hanbok is changing little by little every year, research on the trend of Hanbok is rare. This study analyzed the results of searching for 'Hanbok' as a keyword in Portal Site Naver and posting a customized purchase of Hanbok for marriage between 2016 and 2017. The analysis was based on analyzing the photos uploaded, and analyzing purchase reason in the On-line review. Most buyer of Hanbok purchased for prepare marriage. The choice of a customized hanbok is mostly to search online or to use the fair. The most important factor in choosing a custom Hanbok that appeared in online reviews is color and then price. The color of the jacket is mostly light color and the off-white color is the most used and the long skirt such as the pink system, the chorale system and the red system, and it can be seen that the pink skirt is overwhelmingly large. In the design of Hanbok, The sleeves were straight and narrow, and the length was the chest line. The collar were enlarged and widened. 고름 used the sole color instead of the jacket and skirt color, and it was narrow not long. skirt's pleats was wide, and designed to overlap with double color of the fabric.

• Journal of the Korean Society of Costume
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• v.58 no.5
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• pp.118-133
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• 2008

The basic costumes of the joseon dynasty such as pants and jackets were not particularly distinctive over the period in comparison to po. The coat that was worn over pants and jacket, however, had different forms in the course of the joseon dynasty. In this paper, I will analyze the changing style of pyeonbokpo, the daily costumes of Joseon man, which were excavated in recent years. Changes in the collar style were the most distinctive of joseon pyeonbokpo such as cheolik, dapho, aekjueum, jikryeong, daechangyeui, jungchimak, and juyeui. Although pyeonbokpo had double collars in the early joseon dynasty, they disappeared in the seventeenth century: a straight line also replaced by a curve for the collar perimeter. Front-adjusting of pyeonbokpo was very deep in the early joseon dynasty; front-adjusting became simple in the late joseon dynasty, resulting in the central positioning of a ribbon. The rectangular form of a gusset(mu) was stitched to the bodice through various pleats. Gusset patterns changed greatly into various forms in the seventeenth and eighteenth centuries, along with the ladder gusset form. The long vest(dapho) was half-sleeved and had a deep front adjustment in the early joseon dynasty; however, it did not have collars and sleeves since the eighteenth century. The waist line of cheolik had the ratio of 1.2 to 1 for the bodice to skirt. But the length of skirts grew gradually into the ratios of 1 to 1, 1 to 2, and 1 to 3.8 while the waist line moved upwards to the chest line. Sleeves of all po had a straight form but they gradually developed the tendency to widen towards the wrists. Later, sleeves changed again, having a wide rectangular form starting from the armpits. Cheolik was widely worn in the early joseon dynasty; however, it was worn less in later years. Instead, clothes that had slits such as dopo, daechangeui, and jungchimak were greatly available. These characteristics of the change in men's costumes enable us to measure the time period of excavated findings despite the lack of records for Joseon graves.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.115.1-115.1
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• 2011

We analyzed the line profiles of the planetary nebula (PN) NGC 7009 secured with the Keck I HIES and BOES's spectral data. The 5 positions were taken over the nebular image, 4 points on the bright rim plus 1 point at the central position. The covered spectral wavelength range was $3250{\AA}-8725{\AA}$ in these observations. We decomposed the lines of HI, HeI, HeII, CII, NIII, [ClIII], [NII], [OII], [OIII], [SII], [SIII], [ClIII], and [ArIII] using the IRAF and StarLink/Dipso. After correcting the Earth's movement and the PN's radial velocities, -48.6 & -48.9 km/s, respectively, for the Keck & BOES, we produced the line profiles in a velocity scale. The zero velocity at each line profile clearly indicates which part of the components is approaching or receding, giving a general information of the kinematical structure. Almost all of the low-to-medium excitation lines, such as [NII], [SII], [O III], and [ArIII], secured at the central position and four positions along the major & minor axes, showed 3 components, double peak + a wide wing component, suggesting the fast outflow structures are present. The overall geometry is a prolate shell which also has a fainter outer shell in the halo zone, but there appears to be some peculiar sub-structures inside the main shell. The high excitation He I, HeII, NIII lines which might be formed close to the inner boundary of the shell show unusual features, completely different from the other lines. The HeII and these high excitation lines may be indicative of a relative recent fast outflow from the central star and the permitted lines such as NIII might be affected by the innermost structure. We discuss a possible presence of a jet-like fast outflow structure in an out-flow axis different from the main axis of the spheroid shell.