• Korean Journal of Agricultural Science
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• v.44 no.4
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• pp.549-557
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• 2017

As exchanges between countries become more active, new threats such as drugs, illegal imports of food and medicines, and terrorism are present all over the world. From this, increased border security that protects people's safety is becoming a new issue. The activities of special purpose dogs that detect these threats in advance are becoming very important. One of the obstacles in securing superior individuals is musculoskeletal disorders which interfere with the work of special purpose dogs. In order to search for genes associated with these genetic disorders, we conducted genomic analysis using linkage disequilibrium information and investigated genetic characteristics to know heterozygosity and inbreeding status in the population. In this study, two breeds (Malinois, Shepherd) of army dogs and three breeds (Malinois, Shepherd, Retriever) from public databases were used for comparison. The 170K SNP marker panel was used for this study. In the principal component analysis, it was confirmed that clusters were formed for each breed. The number of effective populations differed for each cultivar, but this was due to the difference in numbers of individuals for each breed used for the analysis. The results of heterozygosity decay analysis showed that heterozygous alleles decreased with each generation. In the army dog group, if the population number is maintained properly, the frequency of allele genotype will not decrease significantly.

• Journal of Veterinary Clinics
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• v.34 no.5
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• pp.318-324
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• 2017

The objective of this study was to establish kinematic reference ranges for the femorotibial (FT) joint and the patellofemoral (PF) joint in healthy small-breed dogs by measuring 3D kinematics at the walk. Single-plane fluoroscopy was used to image the stifle joints of five healthy beagle dogs while the dogs were walking. 3D bone models of the femur, patella, and tibia were reconstructed by computed tomography scanning of the beagle dogs' hind limbs. The shape-matching technique was used to measure kinematic data from the fluoroscopic images and the 3D bone models. The cranial translation of the tibia during walking was inversely proportional to the FT joint flexion. There were significant correlations between the patellar motion and the tibial motion. The FT joint flexion had a strong correlation with the patellar proximodistal translation and flexion. Additionally, the tibial mediolateral translation had a strong correlation with the patellar shift and tilt. In this study, normal in vivo 3D FT joint and PF joint kinematics were demonstrated, and the average kinematic parameters were determined in walking beagle dogs.

• Journal of Veterinary Clinics
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• v.37 no.4
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• pp.175-179
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• 2020

The objective of this study was to determine the effect of osteotomy angle and tibial proximal segment rotation angle on angular and torsional tibial deformities and to assess the trends of these deformities during the rotation of the tibial proximal segment in a center of rotation of angulation (CORA)-based leveling osteotomy (CBLO) by performing computer modeling of the tibia. Four tibias of toy breed dogs with no history of lameness were used in this study. Osteotomies were performed in the proximal tibias at angles of 0°, 10°, 20°, -10°, and -20°, perpendicular to either the proximodistal or craniocaudal tibial axes. The mechanical medial proximal tibial angle (mMPTA) and transcondylar (TC) and distal cranial tibial (CnT) axes were used to measure angular and torsional deformities, respectively. All tibias showed an increase in angular and rotational deformities with an increase in the tibial plateau rotation angle. The tibia with osteotomies performed in the proximodistal and craniocaudal directions showed the highest magnitude of torsional and angular deformities, respectively. The results of this study revealed a tendency of occurrence of angular and torsional deformities with osteotomy performed along the proximodistal and craniocaudal directions in the CBLO.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.8
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• pp.1078-1084
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• 2009

The Jindo dog is a Korean natural monument and is recognized by the Fédération Cynologique Internationale. A prominent feature is the diverse coat color within the breed. To analyze the genetic basis of variation in the Jindo coat color, we sequenced the protein-coding regions of the melanocortin 1 receptor gene (MC1R). The MC1R coding sequence was determined from 154 dogs in five breeds (Jindo, Labrador Retriever, English Springer Spaniel, Belgian Malinois, and German Shepherd). To confirm the genetic structure of sampled populations, we tested for Hardy-Weinberg equilibrium (HWE) and computed $F_{st}$ The sample populations did not significantly deviate from HWE. $F_{st}$ was 0.02 between white and fawn Jindo dogs; this was lower than $F_{st}$ between breeds. Six single nucleotide polymorphisms (SNPs) were detected in the MC1R coding region. Among the six SNPs, five were non-synonymous (S90G, T105A, Q159P, M264V, and R306ter) and one was synonymous SNP (Y298Y). From the SNPs, we predicted four haplotypes (H1, H2, H3, and H4) for Jindo MC1R. Jindo dogs had different haplotypes corresponding to different coat colors. H1 was frequently observed in white Jindo dogs with an odds ratio of 5.03 (95% CI: 2.27-11.18, p<0.0001), whereas H2 and H4 were observed only in fawn Jindo dogs. Our findings indicate that SNP haplotype can influence coat color. Knowledge of MC1R haplotypes can help discriminate white and fawn coats in Jindo dogs. We hope this report will trigger more research into the genetics of this traditional Korean dog and will be a reference for dogs of Asian origin. Also, our results will provide a useful genetic marker for Jindo dog breeders who have selected for specific colors.

• Journal of Veterinary Clinics
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• v.18 no.2
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• pp.124-132
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• 2001

The purpose of this study is to know whether single layer continuous connell suture is an acceptable alternative to simple interrupted approximating suture for end-to-end intestinal anastomosis in dogs. Fourteen mixed-breed dogs weighing 2 to 5 kg were allotted to group treated with simple interrupted approximating suture (Group I) and group treated with single layer continuous Connell suture (Group II), each of 7 dogs. All dogs in each suture pattern were compared with time for total operation ad suture elapsed for intestines to anastomose, clinical signs, changing of pre-and postoperative luminal size, status of feces, adhesion at anastomotic site for 14 days after operation. Time for total operation and suture time for intestinal anastomosis were none significant between Group I and Group II, although those in Group II was about 3 minutes shorter than those in Group I, respectively. Group I spent average 47.08${\pm}$11.10 minutes on total operation, 20.97${\pm}$5.54 minutes on suture time for intestinal anastomosis and Group II spent average 44.74${\pm}$7.77 minutes, 17.73${\pm}$3.05 minutes, respectively. All dogs were no special differences in vitality, vomiting, appetite between Group I and Group II for 14 days after operation. All dogs, except one dog in Group I, had showed normal vitality and appetite since 6~8 days after operation. Initial return of fecal passage showed in all dogs before 6 days after operation and thereafter most dogs showed normal feces. According to results, it was thought that all dogs with normal vitality and appetite before 8 days had showed good prognosis. There were no changes of intestinal luminal size in 2 dogs performed Group In and one dog performed Group II between at operation and 14 days after operation. Narrowing rate of intestinal lumen in Group I was average 9.3% of the normal diameter, whereas in Group II, 9.5% of normal diameter. In complications after operation, only one dog in Group I showed intestinal intussusception but the others didn't. Length of adhesion was measured between intestinal anastomotic site and omental graft. Length of adhesion in dogs performed Group II was mostly shorter than that of Group I. Adhesion with proximate intestines occurred in five dogs, which consisted of 3 dogs performed Group I and 2 dogs performed Group II. Concurrently, they had a great length of adhesion between anastomotic site and omental graft. There were no great differences between Group I and Group II about speed of operation, clinical signs, complications such as leakage and stricture. And all dogs performed intestinal anastomosis showed good clinical condition and prognosis. In conclusion, Single layer continuous Connell suture can safely perform an intestinal anastomosis and be an alternative of simple interrupted approximating suture in aspect of speed clinically.

• Journal of Animal Science and Technology
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• v.50 no.2
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• pp.273-278
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• 2008

There have been studies about dog’s personality and behavior, which is helpful to breed dogs as guide or companion. In this study, a questionnaire was developed using 54 Jindo dogs, which considered ten items about aggressiveness and sociability. The scores were analyzed by principle component analysis (PCA), after accounting for four variables: age, gender, growing place, and coat-colors. Our results from the PCA indicated three principle components, which classified ‘aggressiveness’, ‘sociability’ and unknown factor. The four variables did not significantly affect aggressiveness(P>0.05). However, there was a relationship between coat-color and sociability, i.e., the Jindo dogs with fawn color were more sociable than the white ones(P<0.1).

• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.423-428
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• 2009

There were outbreaks of canine distemper in Korea from the late 1990's to the early 2000's even though modified live CDV vaccines had been used as the same way as before. The present study was undertaken to investigate the levels of neutralizing antibodies in the Korean dog population, and the factors associated with the levels, with special reference to the vaccination history of the dogs. A total of 772 serum samples were from clinically healthy dogs with over one year old throughout the Korea from January 2003 to April 2004. Details on the sex, breed, age, vaccination status and disease histories were recorded. The level of neutralizing antibodies titer was determined with a modified version of the microneutralization test. Titers over 16 were classified as protective CDV antibody titers. The overall rate of adult dogs with protective antibody titers was 96.0%. The dogs with protective antibody titers varied depending on age, sex, rearing environment and vaccination status. Because the majority of healthy adult dogs in Korea had adequate serum antibody titers against CDV and the immunity provided by the vaccinations is claimed to last for several years, annual revaccination protocol for CDV in adult dogs should be reconsidered.

• Journal of Life Science
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• v.18 no.6
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• pp.902-906
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• 2008

In this study, we analyzed the microsatellite DNA poly-morphisms for pedigree verification in Kyungju dog (Dongkyung-i) which is one of the Korean breed dogs. A total of 51 Dongkyung-i samples were genotyped using 8 microsatellite markers. The number of alleles observed at single locus ranged from 4 to 12, with average number of alleles per locus of 8.5. The expected heterozygosity and polymorphic information contents (PIC) values of the 8 microsatellite loci were $0.6162{\sim}0.8746$ (mean 0.7587) and $0.5461{\sim}0.8512$ (mean 0.7167), respectively. Of the 8 markers, PEZ3, PEZ6, PEZ12 and FHC2054 loci had relatively high PIC values (>0.7) in Dongkyung-i. Pedigree verification of Dongkyung-i was analyzed based on alleles observed. The results of the parentage testing were noted significant differences compared with breeders. These results show basic information of conservation and research in Dongkyung-i, and further studies of genetic pedigree in Dongkyung-i will be needed.

• Korean Journal of Veterinary Research
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• v.45 no.3
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• pp.369-373
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• 2005

A male mixed breed dog with unknown age (case 1) and 5-year-old female golden retriever (case 2) were admitted to the Department of Veterinary Pathology at College of Veterinary Medicine, Seoul National University after sudden death. At necropsy, stifle region of case 1 was replaced by coalescing firm tan nodules approximately 1-3 cm in diameter. Neoplastic nodules were also presented in the lung. In case 2, the spleen was enlarged 3-5 times its normal size and numerous firm tan nodules were noted in the kidney, pericardium, diaphragm and lymph nodes. Histopathologically, the neoplastic masses of case 1 consisted of plump spindle cells having abundant cytoplasm and indistinct cell borders that formed interlacing bundle. In case 2, the masses were consisted of round to polygonal cells with abundant eosinophilic cytoplasm and eccentric nuclei. Numerous multinucleated giant cells containing nuclei of various sizes were noted. Immunohistochemically, neoplastic cells of all two cases stained intensely with vimentin and lysozyme. Based on the gross and light microscopic findings together with immunohistochemistry, both cases were diagnosed as localized histiocytic sarcoma with pulmonary metastasis and disseminated histiocytic sarcoma, respectively.

• Journal of Veterinary Clinics
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• v.37 no.4
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• pp.169-174
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• 2020

The purpose of this study was to establish normal values for the thoracic limb joint reference angles in Shih Tzu dogs and to describe the standardized CT methodology for measuring the joint reference angle of the humerus. Five pairs of thoracic limbs of Shih Tzu dogs were collected for the CT scans in this study. Three blinded observers measured the joint reference angle of the humerus and radius for each dog by using CT scans in the frontal, sagittal, and axial planes. The means (± SDs) for the average of the right and left humeral joint reference angles were as follows: mMPHA, 83.74 ± 3.95°; mLDHA, 85.04 ± 2.57°; mCaPHA, 46.75 ± 2.20°; mCrDHA, 79.47 ± 1.97°; and HTA, 19.16 ± 2.38°. Means (± SD) for the average of right and left of the radial joint reference angles were as follows: aMPRA, 85.04 ± 1.58°; aLDRA, 87.59 ± 1.37°; aCrPRA, 84.60 ± 1.46°; aCdDRA, 84.27 ± 1.79°; and RTA, 20.91 ± 3.00°. The intraclass correlation coefficients (ICCs) of the joint reference angles for the inter- and intraobserver reliability were good to excellent, except those for the mCrHA and HTA were moderate. Our results suggest that the method of measuring joint reference angles of other long bones by using CT can be applied to thoracic limbs and can extract valid values for one specific breed.