• Title/Summary/Keyword: Direct quenching

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Mesodermal repression of single-minded in Drosophila embryo is mediated by a cluster of Snail-binding sites proximal to the early promoter

  • Park, Kye-Won;Hong, Joung-Woo
    • BMB Reports
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    • v.45 no.10
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    • pp.577-582
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    • 2012
  • single-minded (sim) is a master regulatory gene that directs differentiation in the central nervous system during Drosophila embryogenesis. Recent identification of the mesectoderm enhancer (MSE) of sim has led to the hypothesis that two Snail (Sna)-binding sites in the MSE may repress sim expression in the presumptive mesoderm. We provide evidence here that three Sna-binding sites proximal to the sim promoter, but not those of the MSE, are responsible for the mesodermal repression of sim in vivo. Using transgenic embryos injected with lacZ transgenes, we showed that sim repression in the mesoderm requires the three promoter-proximal Sna-binding sites. These results suggest that Sna represses the mesectodermal expression of sim by directly repressing the nearby promoter, and not by quenching adjacent transcriptional activators in the MSE. These data also showed how the MSE, lacking the three proximal Sna-binding sites, reproduced the endogenous pattern of sim expression in transgenic embryos.

Elucidation of photo-induced electron transfer in a loop-forming peptide: Dye-Ala-Gly-Gln-Tyr

  • Lee, Hwiin;Kim, Jeongyun;Kwon, Yong-Uk;Lee, Minyung
    • Rapid Communication in Photoscience
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    • v.4 no.3
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    • pp.59-62
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    • 2015
  • We investigated photo-induced electron transfer (PET) in a dye-labeled peptide, fluorophore-Ala-Gly-Gln-Tyr, employing time-resolved fluorescence. As an effort to develop new functional dyes, we studied an acriflavine derivative for the electron-acceptor in the excited state from tyrosine, an electrondonor in the ground-state. The pH dependence of the fluorescence lifetime of the model peptide indicates that electron transfer between the excited dye and tyrosine occurs when the tyrosine is deprotonated. The proton-coupled electron transfer appears to be sequential rather than concerted. We also report direct time measurements on the end-to-end loop formation processes of the peptide in water.

Microstructure and Fracture Path of Cr-Mn-N Steel upon Aging Treatment

  • Lee, Se-Jong;Sung, Jang-Hyun;Ralls, K.M.
    • Journal of the Korean Society for Heat Treatment
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    • v.4 no.3
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    • pp.21-30
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    • 1991
  • Microstructural analysis was conducted to observe the effect of aging treatments in a Cr-Mn austenitic stainless steel containing nitrogen, and the amount, size, shape and distribution of precipitates were investigated. It was found that on water quenching from $1000^{\circ}C$ after holding 3 h at that temperature, the steel contained no precipitates observable by optical microscopy. Precipitation of phases begins at places most favorable for the formation of nuclei-in the boundaries of grains and twins. Precipitates were studied in detail by means of scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Chemical compositions of precipitates were examined by the use of scanning transmission electron microscopy (STEM) together with an energy dispersive X-ray (EDX) microanalysis. Also chromium depletion adjacent to grain boundary precipitates was investigated by the use of Auger electron spectroscopy (AES) for a direct examination of the fracture surface chemistry.

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Improved Luminescence Properties of Polycrystalline ZnO Annealed in Reduction Atmosphere

  • Chang, Sung-Sik
    • Journal of the Korean Ceramic Society
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    • v.48 no.3
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    • pp.251-256
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    • 2011
  • The luminescence properties of polycrystalline ZnO annealed in reducing ambience ($H_2/N_2$) have been studied. An effective quenching of green luminescence with enhanced UV emission from polycrystalline ZnO is observed for the reduced ZnO. The variations of the UV and green luminescence band upon reduction treatment are investigated as a function of temperature in the range between 20 and 300 K. Upon annealing treatment in reducing ambience, the optical quality of polycrystalline ZnO is improved. The UV to green intensity ratio of sintered ZnO approaches close to zero (~0.05). However, this ratio reaches more than 13 at room temperature for polycrystalline ZnO annealed at $800^{\circ}C$ in reducing ambience. Furthermore, the full width at half maximum (FWHM) of the UV band of polycrystalline ZnO is reduced compared to unannealed polycrystalline ZnO. Electron paramagnetic resonance (EPR) measurements clearly show that there is no direct correlation between the green luminescence and oxygen vacancy concentration for reduced polycrystalline ZnO.

Surface modification of materials by thermal plasma (열플라즈마를 이용한 재료의 표면개질)

  • Kang, Seong-Pyo;Lee, Han Jun;Kim, Tae-Hee
    • Journal of the Korean institute of surface engineering
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    • v.55 no.6
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    • pp.308-318
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    • 2022
  • The surface modification and treatment using thermal plasma were reviewed in academic fields. In general, thermal plasma is generated by direct current (DC) and radiofrequency (RF) power sources. Thermal spray coating, a typical commercial process using thermal plasma, is performed by DC thermal plasma, whereas other promising surface modifications have been reported and developed using RF thermal plasma. Beyond the thermal spray coating, physical and chemical surface modifications were attempted widely. Superhydrophobic surface treatment has a very high industrial demand particularly. Besides, RF thermal plasma system for large-area film surface treatment is being developed. Thermal plasma is especially suitable for the surface modification of low-dimensional nanomaterial (e.g., nanotubes) by utilizing high temperature and rapid quenching. It is able to synthesize and modify nanomaterials simultaneously in a one-pot process.

Microstructural, mechanical, and electrochemical analysis of carbon doped AISI carbon steels

  • Muhammad Ishtiaq;Aqil Inam;Saurabh Tiwari;Jae Bok Seol
    • Applied Microscopy
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    • v.52
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    • pp.10.1-10.15
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    • 2022
  • The effect of carbon doping contents on the microstructure, hardness, and corrosion properties of heat-treated AISI steel grades of plain carbon steel was investigated in this study. Various microstructures including coarse ferrite-pearlite, fine ferrite-pearlite, martensite, and bainite were developed by different heat treatments i.e. annealing, normalizing, quenching, and austempering, respectively. The developed microstructures, micro-hardness, and corrosion properties were investigated by a light optical microscope, scanning electron microscope, electromechanical (Vickers Hardness tester), and electrochemical (Gamry Potentiostat) equipment, respectively. The highest corrosion rates were observed in bainitic microstructures (2.68-12.12 mpy), whereas the lowest were found in the fine ferritic-pearlitic microstructures (1.57-6.36 mpy). A direct correlation has been observed between carbon concentration and corrosion rate, i.e. carbon content resulted in an increase in corrosion rate (2.37 mpy for AISI 1020 to 9.67 mpy for AISI 1050 in annealed condition).

CORIUM COOLABILITY UNDER EX-VESSEL ACCIDENT CONDITIONS FOR LWRs

  • Farmer, Mitchell T.;Kilsdonk, Dennis J.;Aeschlimann, Robert W.
    • Nuclear Engineering and Technology
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    • v.41 no.5
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    • pp.575-602
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    • 2009
  • In the wake of the Three Mile Island accident, vigorous research efforts were initiated to acquire a basic knowledge of the progression and consequences of accidents that involve a substantial degree of core degradation and melting. The primary emphasis of this research was placed on containment integrity, with: i) hydrogen combustion-detonation, ii) steam explosion, iii) direct containment heating (DCH), and iv) melt attack on the BWR Mark-I containment shell identified as energetic processes that could lead to early containment failure (i.e., within the first 24 hours of the accident). Should the core melt fail the reactor vessel, then non-condensable gas production from Molten Core-Concrete Interaction (MCCI) was identified as a mechanism that could fail the containment by pressurization over the long term. One signification question that arose as part of this investigation was the effectiveness of water in terminating an MCCI by flooding the interacting masses from above, thereby quenching the molten core debris and rendering it permanently coolable. Successful quenching of the core melt would prevent basemat melt through, as well as continued containment pressurization by non-condensable gas production, and so the accident progression would be successfully terminated without release of radioactivity to the environment. Based on these potential merits, ex-vessel corium coolability has been the focus of extensive research over the last 20 years as a potential accident management strategy for current plants. In addition, outcomes from this research have impacted the accident management strategies for the Gen III+LWR plant designs that are currently being deployed around the world. This paper provides: i) an historical overview of corium coolability research, ii) summarizes the current status of research in this area, and iii) highlights trends in severe accident management strategies that have evolved based on the findings from this work.

Modeling on the Condensation of a Stable Steam Jet Discharging into a Quenching Tank (응축탱크로 방출되는 안정된 증기제트 응축모델)

  • 김환열;하광순;배윤영;박종균;최상민
    • Journal of Energy Engineering
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    • v.10 no.4
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    • pp.349-356
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    • 2001
  • Phenomenon of direct contact condensation (DCC) heat transfer between steam and water is characterized by the transport of heat and mass through a moving steam/water interface. Since the DCC heat transfer provides some advantageous features in the viewpoint of enhanced heat transfer, it is widely applied to the diversified industries. This study proposes a simple condensation model on the stable steam jets discharging into a quenching tank with subcooled water from a single horizontal pipe for the prediction of the steam jet shapes. The model was derived from the mass, momentum and energy equations as well as thermal balance equation with condensing characteristics at the steam/water interface for the axi-symmetric coordinates. The extremely large heat transfer rate at the steam/water interface was reflected in the effective thermal conductivity estimated from the previous experimental results. The results were compared with the experimental ones. The predicted steam jet shape(i. e. radius and length) by the model was increasing as the steam mass flux and the pool temperature were increasing, which was similar to the trend observed in the experiment.

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Imaging of self-assembled monolayers by surface plasmon microscope (표면 플라즈몬 현미경을 이용한 자기조립 단분자막의 이미징)

  • 표현봉;신용범;윤현철;양해식;김윤태
    • Korean Journal of Optics and Photonics
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    • v.14 no.1
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    • pp.97-102
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    • 2003
  • Multi-channel images of 11-MUA(11-Mercaptoundecanoic acid) and 11-MUOH(11-Mercaptoundecanol) self-assembled monolayers were obtained by using two-dimensional surface plasmon resonance (SPR) absorption. The patterning process was simplified by exploiting direct photo-oxidation of thiol bonding (photolysis) instead of conventional photolithography. Sharper images were resolved by using a white light source in combination with a narrow bandpass filter in the visible region, minimizing the diffraction patterns on the images. The line profile calibration of the image contrast caused by different resonance conditions at each point on the sensor surface (at a fixed incident angle) enables us to discriminate the monolayer thickness in nanometer scale. Furthermore, there is no signal degradation such as photo bleaching or quenching, which are common in the detection methods based on fluorescence.

The Study of Low Carbon Microalloyed Forging Steels by Direct Quenching Method with Mo Additions (몰리브덴을 첨가한 직접 소입 저탄소 비조질강에 관한 연구)

  • Wee, Kyoum-Bok;Lee, Kyung-Sub
    • Korean Journal of Materials Research
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    • v.2 no.6
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    • pp.452-460
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    • 1992
  • Effects of the microalloyed elements, temperatures and cooling rates on the strength and toughness of the medium carbon microalloyed hot forging steels obtained by air cooling(A.C.) method and the low carbon microalloyed forging steels by direct quenching(D.Q.) method were investigated. Combined additions of V+Nb produced the optimum combination of strength and toughness with ferrite-pearlite structure of the medium carbon steel by the A.C. method. 831MPa in UTS and 52.1J in toughness were obtained for 0.40c+0.12V+0.07Nb. It was martensite structure for the low carbon steel by the D.Q. method. The highest UTS and toughness obtained by Mo additions were 855MPa and 108j by 0.12C+0.10V+0.03Nb+1.13Mo respectively. Especially, the toughness of the low carbon steel was twice better than that of the medium carbon steel. 110$0^{\circ}C$was more appropriate than 120$0^{\circ}C$ for the reheating and forging temperature and 1.$2^{\circ}C$ /s was the best cooling rate from the viewpoint of the strength and toughness. Multiple regression analysis was used to quantify the influence of the microalloyed elements, temperatures and cooling rates on the strength, toughness, austenite grain size, and the pearlite interlamellar spacing.

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