• 청정기술
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• 제20권4호
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• pp.367-374
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• 2014

0.1~1 M의 철킬레이트 화합물을 이용한 화학흡수반응에 의한 바이오가스내 황화수소제거를 위한 실험이 수행되었다. 철킬레이트 화합물을 이용한 황화수소제거는 철킬레이트의 최적산화반응을 통해 이루어진다. 바이오가스에 존재하는 황화수소는 킬레이트농도 및 pH 등의 공정조건에 따라 효과적으로 제거될 뿐만 아니라 철킬레이트 산화반응에 의해 황화수소내 존재하는 황성분을 생성시킨다. Fe-EDTA의 농도가 증가하면 철킬레이트 화합물의 착물이 안정되어 황생성의 전환이 증가하였다. 또한 철킬레이트화합물의 안정도는 pH에 따라 변하는 중요한 인자이고 pH 9에서 최적반응을 나타냈다.

• 한국식품과학회지
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• 제7권4호
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• pp.212-220
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• 1975

국내에서 새로이 보급된 Indica형 장려품종인 통일미(統一米)와 Japonica형 재래장려품종인 진흥미(振興米)의 취반기호특성(炊飯嗜好特性)을 비교, 검토한 결과는 다음과 같다. 통일미는 진흥미와 비교할 때 취반시(炊飯時) 같은 가수율(加水率)에서 $4{\sim}6%$ 더 많은 수분을 흡수하였고 항상 높은 hardness를 나타내었으며 같은 hardness를 유지하기 위하여 20% 더 많은 가수율(加水率)을 요구하였다. texture특성치(特性値)중 hardness의 역수(逆數)로 부터 취반시(炊飯時) 최적가수율(最適加水率)을 구한 바 통일미는 140%, 진흥미는 120%이었다. 미반(米飯)의 호화도(糊化度)는 노화도(老化度)를 효소소화법( 酵素消化法), 요오드정색법(呈色法), texture측정법에 의하여 측정한 결과, 같은 가수율(加水率)에서 통일미는 진흥미보다 항상 낮은 호화도(糊化度)를 나타낸 반면 노화도(老化度)는 약간 떨어지는 경향이 있었다. 미분(米粉)의 amylogram 특성(特性)을 보면 통일미는 진흥미에 비하여 호화개시온도(糊化開始溫度)는 높은 반면 최고 점도(粘度)와 breakdown은 낮은 값을 나타내었다.

• 동의생리병리학회지
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• 제23권6호
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• pp.1267-1272
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• 2009

The purpose of this study is to survey the importance of items for the physiological and pathological symptoms for estimating a health condition according to Sasang constitution to establish the SHI(Sasangin Health Index) which will reflect the concept of inherent vitality. We surveyed expert opinion with questionnaires from 20 Sasang constitution specialists. The questionnaire was composed of 57 items on physiological and pathological symptoms and specialist opinions. Each item was marked from A to E according to importance in evaluating health state in each constitution, and if the mean score of an item was over 3.0, the item was regarded as important. Important indexes among physiological symptoms were diet, digestion condition, perspiration condition, frequency of defecation, heat and cold response, and temperature of drinking water in Soeumin, pathologic perspiration and defecation condition in Soyangin, repast, perspiration condition, and the amount of drinking water in Taeeumin, and urination frequency in Taeyangin. Important indexes among pathological symptoms were sighing, indigestion, and abdominal pain in Soeumin, oral condition, chest distress, brash, and amnesia in Soyangin, eye condition, palpitation, and edema in Taeeumin, and vomiting and incapacity of the lower limbs in Taeyangin. There are different health evaluating index and priority order in it according to Sasang constitution.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.262-270
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• 2007

Of the twelve lytic bacteriophages recovered from five different fermenting cucumber tanks that were inoculated with Pediococcus sp. LA0281, a lytic phage, ${\phi}ps05$, was characterized in the present study. The plaques were mostly clear and round-shaped on the lawn of starter strain, indicating lytic phage. Overall appearance indicated that it belongs to the Siphoviridae family or Bradley's group B1, with a small isometric head and a flexible noncontractile tail with swollen base plate. The average size was found to be 51.2 nm in head diameter and 11.6 nm wide ${\times}$ 129.6 nm long for the tail. The single-step growth kinetics curve showed that the eclipse and the latent period were 29 min and 34 min, respectively, and an average burst size was calculated to be 12 particles per infective center. The optimum proliferating temperature ($35^{\circ}C$) was slightly lower than that of cell growth ($35\;to\;40^{\circ}C$). The structural proteins revealed by SDS-PAGE consisted of one main protein of 33 kDa and three minor proteins of 85, 58, and 52 kDa. The phage genome was a linear double-stranded DNA without cohesive ends. Based on the single and double digestion patterns obtained by EcoRI, HindIII, and SalI, the physical map was constructed. The overall size of the phage genome was estimated to be 24.1 kb. The present report describes the presence of a lytic phage active against a commercial starter culture Pediococcus sp. LA0281 in cucumber fermentation, and a preliminary study characterizes the phage on bacterial successions in the process of starter-added cucumber fermentation.

• 미생물학회지
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• 제25권4호
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• pp.293-303
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• 1987

혐기성 Clostrium therrnocellurn의 배양액으로부터 hydroxyapatite column chromatography를 통하여 cellulase complex중의 $C_{1}$ enriched fraction을 분리하였다. 다은 호기성 미생물과 마찬가지로 분리된 $C_{x}$, fraction과 다른 $C_{x}$ 성분과의 synergism에 의해 볼용성 성유소의 분해가 현저히 촉진되였다. $C_{x}$ 성분과는 달리 $C_{1}$, fraction은 공기중에서 산화에 의해 잘 실활되었으나 환원제, 특히 $\beta$-mercaptoethanol에 의해 효소 환성이 강하게 증가되는 것으로 보아 $C_{1}$ component는 다량의 sulfhydryl grou을 가지고 있는 것으로 판단되었다. 이 fraction은 열에 매우 안정하였으며 최적 온도와 pH는 각각 $60^{\circ}C$와 6.0 이었다.

• Journal of Dairy Science and Biotechnology
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• 제36권4호
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• pp.220-225
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• 2018

프로바이오틱스의 배양 및 동결건조 과정에서 프로바이오틱스가 사멸하지 않을 정도의 초음파 처리를 통하여 프로바이오틱스의 생존율 및 안정성이 높아지고, 코팅제가 골고루 분산되어 코팅 효율이 높아져 프로바이오틱스의 유통안정성 및 소화 생존율이 개선됨을 확인하였다.

• 한국콘텐츠학회논문지
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• 제19권7호
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• pp.166-175
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• 2019

전통의학의 체질론은 현대의학과는 구분되는 특성으로, 사상의학은 건강유지의 관점을 폐비간신(肺脾肝腎)의 기능적 강약(强弱)으로 구분하여 인체 장부(臟腑)의 체질에 따른 기능적 균형이 어떠한 조건에서 불균형이 되는 상태를 병의 주요한 요인으로 파악하였다. 아유르베다는 우리 몸에 존재하는 3개 "도샤(Dosha)'의 균형을 건강 유지의 기본으로 생각하고 질병이 생기는 이유를 체내 에너지 조절 기능을 하는 바따(Vata), 신진대사와 소화 기능을 담당하는 삣타(Pitha), 체온조절 기능을 담당하는 카파(Kapha)의 균형이 깨어졌기 때문으로 보았고 세가지 도샤(tridosha)가 균형을 맞추면 스스로 치유된다고 하였다. 체질에 따른 질병의 특징과 경향을 알아 치료에 접근하고 일상생활의 건강관리까지 포함하고 있는 두 이론은 각 개인의 특성과 독립적이고 자율적인 생활습관이 중시되는 현대사회에서 일상 생활 건강 유지 및 예방을 위해 다양한 예방 치료법의 융합영역으로 해석될 수 있다고 생각된다. 본 연구에서는 사상 의학과 아유르베다 이론을 체질 관점에서 비교 분석하여 환자의 치유력과 회복력에 도움이 되고, 환자의 만족도 증가를 위한 체질 기반 개념을 이해하고 예방 의료분야에 있어서의 활용의 다양성에 대해 도움이 되고자 한다.

• Animal Bioscience
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• 제35권2호
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• pp.308-316
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• 2022

Objective: This study was conducted to determine reduction of various odorous materials from a swine farm equipped with a continuous pit recirculation system (CPRS) with aerobically treated liquid manure. Methods: The CPRS is used in swine farms in South Korea, primarily to improve air quality in pig houses. In this study, CPRS consists of a manure aerobic treatment system and a fit recirculation system; the solid fraction is separated and composted, whereas the aerobically treated liquid fraction (290.0%±21.0% per day of total stored swine slurry) is continuously returned to the pit. Four confinement pig barns in three piggery farms were used; two were equipped with CPRS and the other two operated a slurry pit under the slatted floor. Results: All chemical contents of slurry pit manure in the control were greater than those of slurry pit manure in the CRPS treatment (p<0.05). Electrical conductivity and pH contents did not differ among treatments. The biological oxygen demand of the slurry pit treatment was greater than that of the other treatments (p<0.05). Total nitrogen, total phosphorus, and ammonia nitrogen contents of the slurry pit treatment were greater than those of other treatments (p<0.05). Odor intensity of the CPRS treatment was lower than that of the control at indoor, exhaust, and outside sampling points (p<0.05). The temperature and carbon dioxide of the CPRS treatment in the pig barn was significantly lower than those of control (p<0.05). All measured odorous material contents of the CPRS group were significantly lower than those of the control group (p<0.05). Conclusion: The CPRS application in pig farms is considered a good option as it continuously reduces the organic load of animal manure and lowers the average odorant concentration below the threshold of detecting odorous materials.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.800-807
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• 2022

Four aprE genes encoding alkaline serine proteases from B. subtilis strains were used as template genes for family gene shuffling. Shuffled genes obtained by DNase I digestion followed by consecutive primerless and regular PCR reactions were ligated with pHY300PLK, an E. coli-Bacillus shuttle vector. The ligation mixture was introduced into B. subtilis WB600 and one transformant (FSM4) showed higher fibrinolytic activity. DNA sequencing confirmed that the shuffled gene (aprEFSM4) consisted of DNA mostly originated from either aprEJS2 or aprE176 in addition to some DNA from either aprE3-5 or aprESJ4. Mature AprEFSM4 (275 amino acids) was different from mature AprEJS2 in 4 amino acids and mature AprE176 in 2 amino acids. aprEFSM4 was overexpressed in E. coli BL21 (DE3) by using pET26b(+) and recombinant AprEFSM4 was purified. The optimal temperature and pH of AprEFSM4 were similar to those of parental enzymes. However, AprEFM4 showed better thermostability and fibrinogen hydrolytic activity than the parental enzymes. The results indicated that DNA shuffling could be used to improve fibrinolytic enzymes from Bacillus sp. for industrial applications.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.286-286
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• 2022

The properties of starch play an important role in determining the palatability of rice. In addition, the gelatinization temperature (GT) of rice starch is an important factor in determining the quality of rice because it is related to the cooking time and texture of rice. For the development of high-quality rice, it is important to understand the genetic basis of palatability-related traits, and QTL analysis is an effective method to explain the genetic basis of variation in complex traits. QTL mapping related to alkali digestion value (ADV) of brown and milled rice was performed using the 120 Cheongcheong/Nagdong double haploid (CNDH) line. As a result, 12 QTLs related to ADV were detected, and 20 candidate genes were selected from the RM588-RM1163 region of chromosome 6 through screening by gene function analysis. The comparison of the relative expression level of candidate genes showed that OsSS1q6 is highly expressed in CNDH lines with high ADV in both brown rice and milled rice. In addition, OsSS1q6 has high homology with starch synthase 1 protein, and interact with various starch biosynthesis-related proteins, such as GBSSII, SBE, and APL. Therefore, we suggest that OsSS1q6 identified through QTL mapping could be one of the various genes involved in the GT of rice by regulating starch biosynthesis. This study can be used as basic data for breeding high-quality rice and provides a new genetic resource that can increase the palatability of rice.