Journal of Dairy Science and Biotechnology

Korean Society of Dairy Science and Biotechnology (한국낙농식품응용생물학회)
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Shelf-Life Extension and Increase in Survivability of Probiotics Powder by Ultrasonic Treatment

초음파를 이용한 프로바이오틱스 분말의 유통기한 연장 및 생존율 증대 효과

  • Hong, Dong-Ki (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Jung, Seong-Eun (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Lee, Myoung-Hee (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Lee, Ho-Jin (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Lee, Jae-Ho (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Na, Guk-Nam (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Choi, Il-Dong (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Lee, Jung-Lyoul (Research and Business Development Center, Korea Yakult Co., Ltd.) ;
  • Sim, Jae-Hun (Research and Business Development Center, Korea Yakult Co., Ltd.)
  • 홍동기 ((주)한국야쿠르트 중앙연구소) ;
  • 정성은 ((주)한국야쿠르트 중앙연구소) ;
  • 이명희 ((주)한국야쿠르트 중앙연구소) ;
  • 이호진 ((주)한국야쿠르트 중앙연구소) ;
  • 이재호 ((주)한국야쿠르트 중앙연구소) ;
  • 나국남 ((주)한국야쿠르트 중앙연구소) ;
  • 최일동 ((주)한국야쿠르트 중앙연구소) ;
  • 이정열 ((주)한국야쿠르트 중앙연구소) ;
  • 심재헌 ((주)한국야쿠르트 중앙연구소)
  • Received : 2018.12.20
  • Accepted : 2018.12.25
  • Published : 2018.12.31
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Abstract

The purpose of this study was to investigate the effect of ultrasonic treatment during probiotics production process on the shelf life and the survival rate. Once Lactobacillus plantarum and Bifidobacterium longum were cultured in edible culture medium, ultrasonic treatment was performed at 100 Hz for 1, 2, 5, 7, and 10 minutes. Ultrasonic-treated L. plantarum HY7715 and B. longum HY8001 were centrifuged, mixed with a cryoprotectant, and lyophilized. The prepared lactic acid bacteria powder was individually packaged. After 6 months, viable cell counts were measured separately under cold storage and room temperature. In vitro digestion experiments were performed to determine the survival rate at digestive tract. As a result, it was observed that shelf life and survival rate were increased compared to untreated control group.

프로바이오틱스의 배양 및 동결건조 과정에서 프로바이오틱스가 사멸하지 않을 정도의 초음파 처리를 통하여 프로바이오틱스의 생존율 및 안정성이 높아지고, 코팅제가 골고루 분산되어 코팅 효율이 높아져 프로바이오틱스의 유통안정성 및 소화 생존율이 개선됨을 확인하였다.

Keywords

References

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