• 한국발생생물학회지:발생과생식
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• 제17권3호
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• pp.221-229
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• 2013

Cathepsins are members of the multigene family of lysosomal cysteine proteinases and have regulated function in several life processes. The potential role of cathepsin F cysteine gene was expected as protease in the yolk processing mechanism during early developmental stage, but expression analysis was unknown after fertilization. The alignment analysis showed that amino acid sequence of cathepsin F from olive flounder liver expressed sequence tag (EST) homologous to cathepsin F of other known cathepsin F sequences with 87-98% identity. In this study, we examined the gene expression analysis of cathepsin F in various tissues at variety age flounder. Tissue distribution of the cathepsin F mRNA has been shown to be ubiquitous and constitutive pattern regardless of age in each group, although derived from cDNA library using liver sample. The mRNA level of cathepsin F more increased as developmental proceed during embryogenesis and early developmental stage, especially increased in the blastula, hatching stage and 3 days post hatching (dph). As a result, it may suggest that the proteolysis of yolk proteins (YPs) has been implicated as a mechanism for nutrient supply during early larval stages in olive flounder.

• 대한수학교육학회지:수학교육학연구
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• 제15권3호
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• pp.353-374
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• 2005

본 연구는 수학교육의 연구 방법론에 대한 많은 변화와 더불어 교과과정 개발의 과학적 접근에 대한 필요성이 증대되는 수학교육 연구 경향에 비추어, RME의 개발연구를 고찰함으로써, 우리나라의 좀더 발전적인 수학 교과과정 개발을 위한 시사점을 제시하는 데 그 목적이 있다. 이러한 목적을 달성하기 위하여 RME 개발연구의 배경과 이론적 틀, 개발연구의 목표, 절차, 자료수집, 자료분석, 정당화 과정을 포함한 개발연구의 방법론에 대해 살펴보았다. 마지막으로 우리나라의 수학 교과과정 개발의 개선을 위해 수학교육의 이론과 실제를 반영한 교육과정 문서의 구성, 교과과정 개발 배경에 대한 충실한 보고, 교과과정 개발 절차 개정의 필요성을 논하였다.

• Toxicological Research
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• 제22권2호
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• pp.127-133
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• 2006

Recently, we have reported that the environmental pollutant 2-bromopropane (2-BP) induces a significant embryo-fetal developmental toxicity in rats. However, the cause of developmental toxicity and the relationship between maternal and developmental toxicities could not be elucidated because the developmental toxicity of 2-BP was observed only in the presence of maternal toxicity The in vitro teratogenicity study using whole embryo culture was carried out to understand the teratogenic properties and the possible mechanism of teratogenicity induced by 2-BP in rats. Rat embryos aged 9.5 days were cultured in vitro for 48 hrs at medium concentrations of 0, 1, 3, or 10 mg/ml of 2-BP. Embryos were evaluated for growth, differentiation, and morphological alterations at the end of the culture period. At 10 mg/ml, 2-BP caused a delay in the growth and differentiation of embryos and an increase in the incidence of morphological alterations, including altered yolk sac circulation, abnormal axial rotation, craniofacial hypoplasia, open neuropore, absent optic vesicle and kinked somites. At 3 mg/ml, only a delay in the growth and differentiation of embryos was observed. There were no adverse effects on embryonic growth and development at the concentration of 1 mg/ml. The results showed that the exposure of 2-BP to rat embryos results in a developmental delay and morphological alterations at dose levels of 3 mg/ml culture media or higher and that 2-BP can induce a direct developmental toxicity in rat embryos.

• Reproductive and Developmental Biology
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• 제33권1호
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• pp.29-33
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• 2009

The brilliant cresyl blue (BCB) has been used to select the developmental competent oocytes in pigs, goats and cows. Growing oocytes have a higher level of active glucose-6-phosphate dehydrogenase(G6PDH) compare to mature oocytes and are rarely stained compared to mature oocytes, because G6PDH converts BCB to colorless. First polar body extrusion regard as a guideline of meoisis completion. Selection of polar body extrude oocyte is more developmental competent to blastocyst than unselected. This study was conducted to compare the BCB test to the polar body extrusion on selection of developmental competent porcine oocytes for the production of blastocyst. Cumulus-Oocytes complex were exposed to 26uM BCB stain diluted in NCSU-23 for 90 min. There was no significant difference embryo development to blastocysts between BCB treated and not treated($19.58{\pm}1.99$ vs $18.75{\pm}2.27%$), which means there was no detrimental effect of BCB exposure to oocytes. Normal fertilization is not differed among treatment groups from 70.0 to 78.4% development to blastocyst, beside polyspermy did not. To compare two different selection methods, BCB test and polar body extrusion, evaluate the developmental competent of IVP embryos. BCB+PB+(blue stained and polar body extruded, $20.71{\pm}0.45%$) and BCB-PB+(colorless and polar body extruded, $20.04{\pm}l.29%$) groups are significantly (p<0.05) higher developed than those of BCB+PB-(blue stained and no polar body, $13.24{\pm}0.73%$) and BCB-PB-(colorless and no poladbody, $7.25{\pm}0.77%$). These results showed that selection of polar body extruded oocytes method is more efficient than that of BCB test.

• Child Health Nursing Research
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• 제20권4호
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• pp.350-358
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• 2014

목적 본 연구는 미숙아에게 발달관련 합병증을 예방하고 최적의 성장발달을 촉진하기위한 발달지지간호 개념을 탐색하기위해 시도되었다. 방법 Schwartz-Barcott와 Kim (2000)의 개념 분석 방법을 사용하였으며 이론적 단계, 현장작업 단계, 최종분석 단계에 따라 개념을 분석하였다. 현장작업단계에서는 참여자와 심층면담방법을 통해 자료를 수집하였다. 결과 발달지지간호 개념의 속성은 간호실무차원과 가족중심차원인 2차원으로 구분될 수 있으며, 간호실무차원은 자궁 내 환경 따라가기, 상호작용, 개별화, 발달에 대한 인식을 간호에 통합 등의 4가지 속성으로 도출되었으며, 가족중심차원은 전문적인 협력, 부모-아기 애착지지인 2가지 속성으로 도출되었다. 결론 본 연구에서는 혼종모형을 사용한 개념분석을 통해 미숙아 발달지지간호에 대한 6가지 속성을 추출하였다. 아직 명확하게 제시되지 않은 미숙아 발달지지간호에 대한 개념정의를 제시함으로써 미숙아 발달지지간호 관련 이론 개발 및 실무 증진에 활용될 수 있다고 본다.

• Archives of Pharmacal Research
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• 제29권10호
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• pp.890-897
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• 2006

In inflammatory responses, induction of cytokines and other immune regulator genes in macrophages by pathogen-associated signal such as lipopolysaccharide (LPS) plays a crucial role. In this study, the gene expression profile changes by LPS treatment in the macrophage/monocyte lineage cell line RAW264.7 was investigated. A 60-mer oligonucleotide microarray of which probes target 32381 mouse genes was used. A reverse transcription-in vitro translation labeling protocol and a chemileuminescence detection system were employed. The mRNA expression levels in RAW264.7 cells treated for 6 h with LPS and the control vehicle were compared. 747 genes were up-regulated and 523 genes were down-regulated by more than 2 folds. 320 genes showing more than 4-fold change by LPS treatment were further classified for the biological process, molecular function, and signaling pathway. The biological process categories that showed high number of increased genes include the immunity and defense, the nucleic acid metabolism, the protein metabolism and modification, and the signal transduction process. The chemokine-cytokine signaling, interleukin signaling, Toll receptor signaling, and apoptosis signaling pathways involved high number of genes differentially expressed in response to LPS. These expression profile data provide more comprehensive information on LPS-target genes in RAW264.7 cells, which will be useful in comparing gene expression changes induced by extracts and compounds from anti-inflammatory medicinal herbs.

• 대한간호학회지
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• 제46권6호
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• pp.793-803
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• 2016

Purpose: Developmental care has been recognized as a very important component for the development and health promotion of preterm infants. However, research on how to assess developmental nursing competency has not been studied as expected. This study was done to develop and evaluate a new scale to measure nursing competency for developmental support of preterm infants. Methods: Concept analysis was done with using the Hybrid model of Schwartz-Barcott and Kim (2000), from which a preliminary new scale (30 items) was developed. To test the validity and reliability of the new scale being developed, data were collected from 122 NICU nurses at 4 hospitals in 3 cities in the Republic of Korea, from December, 2014 to March, 2015. Results: The final version of the Developmental Support Competency Scale for Nurses (DSCS-N) caring for premature infants was a 4-point Likert type scale, consisting of 19 items, and categorized as 6 factors, explaining 62.5% of the total variance. Each of the factors were named as follows; 'environmental support' (4 items), 'parental support' (3 items), 'interaction' (3 items), 'critical thinking' (3 items), 'professional development' (3 items), and 'partnership' (3 items). The Cronbach's ${\alpha}$ coefficient for the scale was .83 and the reliability of the subscales ranged from .60~.76. Conclusion: The psychometric evaluation of the new scale demonstrated an acceptable validity and reliability. Findings indicate that the DSCS-N can be used as the tool to test the effect of educational programs for nurses and contribute to advance developmental care for preterm infants.

• 대한지역사회작업치료학회지
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• 제5권1호
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• pp.63-69
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• 2015

목적 : 본 연구는 발달장애 아동을 대상으로 가상현실게임 프로그램이 눈-손 협응력에 어떤 영향을 주는지에 대해 알아보고자 한다. 연구방법 : 발달장애아동 6명을 대상으로 그 중에 3명을 감각통합 프로그램과 가상현실게임 프로그램을 실시하는 실험군으로 선정하였고, 나머지 3명의 아동들은 감각통합 프로그램만을 실시는 대조군으로 선정하였다. 2013년 9월부터 10월까지, 총 10회의 프로그램을 실시하였다. 한국판 시지각 발달검사(K-DTVP-2) 중 하위 항목인 눈-손 협응을 사전-사후평가로 실시하여 결과로 나온 점수를 비교분석하였다. 결과 : 가상현실프로그램에 참여한 아동이 가상현실 프로그램 참여전보다 참여후가 눈-손 협응 능력의 평균점수가 높았고 통계적으로 유의한 차이를 보였다(p>0.044). 감각통합 프로그램에 참여한 아동이 감각통합 프로그램 참여 전보다 참여 후가 눈-손 협응 능력의 평균점수가 높았으나 통계적으로 유의한 차이를 보이지 않았다. 실험군과 대조군 간에는 통계적으로 유의한 차이를 보이지 않았다. 결론 : 가상현실게임 프로그램을 이용한 훈련이 발달장애 아동의 눈-손 협응력 향상에 영향을 주었다.

• 한국수정란이식학회지
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• 제30권3호
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• pp.229-237
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• 2015

To improve the developmental potential of bovine somatic cell nuclear transfer (SCNT) embryos, this study compared the developmental rates to blastocyst stage in the SCNT embryos using donor fibroblasts treated with 5-azacytidine (5AC) and S-adenosylhomocysteine (SAH) at different concentrations. Their reprogramming efficiency level was investigated with level of telomerase activity. Donor fibroblasts isolated from adult ear skin of a cow were exposed to 5AC and SAH at different concentrations during 2 passages. After nuclear transfer into enucleated recipient oocytes, the cleavage and developmental rates were significantly (p<0.05) decreased in the SCNT embryos using 5AC-treated fibroblasts (5AC-SCNT embryos), compared with those of non-treated control (control-SCNT embryos) and SAH-treated fibroblasts (SAH-SCNT embryos). The developmental rates to blastocyst stage tended to be slightly increased in the SAH-SCNT embryos at each of the concentrations, and especially, the developmental rates in the SCNT embryos using 1.0 mM SAH-treated fibroblasts were significantly (p<0.05) higher than that of control SCNT embryos. The mean numbers of total and ICM cell in blastocysts were also significantly (p<0.05) decreased in the 5AC-SCNT embryos, compared with those of other SCNT blastocysts. Further, the level of telomerase activity was also significantly (p<0.05) decreased in the 5AC-SCNT embryos than those of control and SAH-SCNT embryos. Whereas, a significantly (p<0.05) up-regulated telomerase activity was observed in SAH-SCNT embryos, compare with that of control-SCNT embryos. In conclusion, SCNT embryos using hypomethylated donor cells with SAH, not 5AC, may improve the developmental potential and reprogramming efficiency.

• 산업융합연구
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• 제19권4호
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• pp.75-84
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• 2021

본 연구는 취업한 발달장애인의 장애수용이 생활만족도에 미치는 영향에서 직무만족도와 대인관계의 매개효과를 검증하고자 하였다. 이를 위해 장애인고용패널 2차 웨이브 중 의사소통 가능 정도의 측정이 가장 높은 2019년도 4차 조사 데이터를 사용하여 구조방정식 모형 분석을 수행하여 다음과 같은 결과를 도출하였다. 첫째, 취업한 발달장애인의 장애수용 정도는 생활만족도에 정(+)적인 영향을 미치는 것으로 확인됐다. 둘째, 취업한 발달장애인의 장애수용 정도는 생활만족도에 미치는 영향에서 직무만족도는 매개한다. 셋째, 취업한 발달장애인의 장애수용 정도는 생활만족도에 미치는 영향에서 대인관계는 매개한다. 이상의 연구결과를 토대로 함의 및 제언을 제시하였다.