Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Teratogenicity Evaluation of 2-Bromopropane Using Rat Whole Embryo Culture

랫드 전배아배양법을 이용한 2-Bromopropane의 최기형성 평가

  • Kim Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Shin Dong-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Yang Young-Soo (College of Veterinary Medicine, Chonnam National University) ;
  • Oh Ki-Seok (College of Veterinary Medicine, Chonnam National University) ;
  • Jiang Cheng-Zhe (Center for Safety Evaluation and Research of Drugs, Institute of Laboratory, Animal Science. CAMS, Peking Union Medical College) ;
  • Chung Moon-Koo (Korea Institute of Toxicology, KRICT)
  • Published : 2006.06.01
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Abstract

Recently, we have reported that the environmental pollutant 2-bromopropane (2-BP) induces a significant embryo-fetal developmental toxicity in rats. However, the cause of developmental toxicity and the relationship between maternal and developmental toxicities could not be elucidated because the developmental toxicity of 2-BP was observed only in the presence of maternal toxicity The in vitro teratogenicity study using whole embryo culture was carried out to understand the teratogenic properties and the possible mechanism of teratogenicity induced by 2-BP in rats. Rat embryos aged 9.5 days were cultured in vitro for 48 hrs at medium concentrations of 0, 1, 3, or 10 mg/ml of 2-BP. Embryos were evaluated for growth, differentiation, and morphological alterations at the end of the culture period. At 10 mg/ml, 2-BP caused a delay in the growth and differentiation of embryos and an increase in the incidence of morphological alterations, including altered yolk sac circulation, abnormal axial rotation, craniofacial hypoplasia, open neuropore, absent optic vesicle and kinked somites. At 3 mg/ml, only a delay in the growth and differentiation of embryos was observed. There were no adverse effects on embryonic growth and development at the concentration of 1 mg/ml. The results showed that the exposure of 2-BP to rat embryos results in a developmental delay and morphological alterations at dose levels of 3 mg/ml culture media or higher and that 2-BP can induce a direct developmental toxicity in rat embryos.

Keywords

References

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