• 한국발생생물학회지:발생과생식
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• 제26권3호
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• pp.107-115
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• 2022

Kisspeptins, products of KISS1 gene, are ligands of the G-protein coupled receptor (GPR54), and the kisspeptin-GPR54 signaling has an important role as an upstream regulator of gonadotropin releasing hormone (GnRH) neurons. Interestingly, extrahypothalamic expressions of kisspeptin/GPR-54 in gonads have been found in primates and experimental rodents such as rats and mice. Hamsters, another potent experimental rodent, also have a kisspeptin-GPR54 system in their ovaries. The presence of testicular kisspeptin-GPR54 system, however, remains to be solved. The present study was undertaken to determine whether the kisspeptin is expressed in hamster testis. To do this, reverse transcription-polymerase chain reactions (RT-PCRs) and immunohistochemistry (IHC) were employed. After the nest PCR, two cDNA products (320 and 280 bp, respectively) were detected by 3% agarose gel electrophoresis, and sequencing analysis revealed that the 320 bp product was correctly amplified from hamster kisspeptin cDNA. Modest immunoreactive (IR) kisspeptins were detected in Leydig-interstitial cells, and the weak signals were detected in germ cells, mostly in round spermatids and residual bodies of elongated spermatids. In the present study, we found the kisspeptin expression in the testis of Syrian hamster. Further studies on the local role(s) of testicular kisspeptin are expected for a better understanding the physiology of hamster testis, including photoperiodic gonadal regression specifically occurred in hamster gonads.

• 대한소아치과학회지
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• 제50권4호
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• pp.421-433
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• 2023

This study was to examine the developmental dental abnormalities in childhood cancer survivors. Risk factors were assessed for 125 children with radiographic data through a retrospective analysis of medical records and panoramic images. 68.0% of childhood cancer survivors exhibited at least one dental abnormality. The types of abnormalities varied depending on the age at cancer diagnosis and treatment intensity, ranging from microdontia (43.2%), to abnormal root development (39.2%) and tooth agenesis (33.6%). Logistic regression analysis demonstrated that a young age at diagnosis (under 3 years), the use of heavy metal agents, a history of hematopoietic stem cell transplantation (HSCT), and combination treatment of chemotherapy, radiation therapy, and HSCT were associated with a significantly higher risk for overall dental abnormalities. The increased risk ratios were 6.00, 3.06, 3.22, and 7.87, respectively (p < 0.05). The results of this study will predict dental abnormality in permanent dentition according to the diagnosis age and treatment method of childhood cancer.

• Human Ecology Research
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• 제58권2호
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• pp.255-266
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• 2020

This study observed and investigated the commute time of dual-earner couples from a family developmental perspective. It tested whether the household responsibility hypothesis was effective in explaining the gender gap in the commute time for these couples. I extracted 2,103 time diaries written by 1,266 matched couples from the 2014 Korean Time Use Survey data for this study. The sample was categorized by the age of the eldest child into four age groups of 0-6, 7-12, 13-18, and 19-29. Analytic results from ANOVA, Scheffé test, and OLS regression are as follows. First, husbands traveled longer hours to work than wives in all age groups, while the commute time of couples tended to increase along with the child' age. However, couples in the child group aged 7 to 12 had the shortest commute time. Second, domestic labor time of wives were negatively associated with the commute time, which appeared to support the household responsibility hypothesis. Third, in the child group aged 7 to 12, wives spent more time for work commute as their income increased; however, wives with traditional gender role attitudes had a shorter commute time in the child group aged 0 to 6. Forth, neither the wives' nor husbands' work characteristics were related to the wives' commute time; however, both wives' and husbands' work characteristics were related to the husbands' commute time. The findings suggest the possibility of spatial entrapment by working wives throughout the family life cycle due to household responsibilities, which provides implications for policy intervention in consideration of the gender gap in commute time for dual-earner couples.

• 한국직업건강간호학회지
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• 제29권1호
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• pp.49-57
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• 2020

Purpose: This study aimed to identify and compare the risk factors of depression among middle-class Korean workers. Methods: A cross sectional study was designed for secondary data analysis. From the 8th Korean Medical Panel Survey (2008~2013), a total of 3,056 data was drawn and analyzed. With SPSS version 24, a developmental stage comparison, with the stage being young adults (20~39), middle-aged adults (40~64), and older adults (65+) were conducted. Frequency, percentage, 𝑥² test and logistic regression analysis were statistical tools used to analyze the data. Results: In all developmental groups, experience of frustration was found to be a common risk factor of depression. Stress from excessive task, peer-compared subjective health status, and self-perceived social class were risk factors of depression in the young adults and the middle-aged adults. Anxiety for the future significantly influenced depression in the middle-aged adults and older adults. Conclusion: Experience of frustration was a major risk factor of depression among Korean middle-class workers. Interventions to reduce depression need to be developed focusing on the specific risk factors by developmental stages such as experience of frustration, stress from task burden, poor peer-compared subjective health status and anxiety for the future.

• 한국가금학회지
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• 제21권3호
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• pp.175-182
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• 1994

An experiment was conducted to establish a large scale production method of anti-serum against chicken IgM and to profile the developmental changes of serum IgM levels during the feeding period(from hatching to 7 weeks of age) in broiler chicks. Blood samples were taken from Hubbard chicken at the age of hatching, three days of age, and weekly thereafter till to 7 weeks of age. The pure IgM was isolated from ammonium sulfate treated chicken serum by both sephadex G-200 and sepharose CL-6B chromatography. The breaking-through peak containing IgM appeared from the fraction 26 to 28. These fractions consisted mainly of IgM when tested by anti-chicken IgM(Nordic, Netherlands). Immunized with the heavy chain of this purified IgM, the rabbit immune sera(anti-chicken IgM) were formed a reaction only with the purified chicken IgM. The quantitative assay of serum IgM were carried by RID method. The optimal time for diffusion was 14 hours and the coefficient of determination($R^{2}$) for regression equation of standard curve was 0.992. It was observed that IgM concentrations were the highest at hatching(3.23 mg /mL), after that decreased gradually. From 2 to 5 weeks of age the levels unchanged(2.0 ~ 2.3mg /mL), and gradually decreased to 7 weeks of age(1.3 mg /mL).

• 한국보육학회지
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• 제18권1호
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• pp.53-76
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• 2018

본 연구는 보육정책이 가진 아동 발달적 기능에 초점을 두고, 돌봄의 유형이 영아의 발달에 미치는 영향을 살펴보고자 했다. 구체적으로는 돌봄의 유형을 어린이집 돌봄, 가정내 양육으로 구분하고 이를 세분화하여 각 발달영역에 미치는 영향을 비교하고자 하였다. 한국아동패널 1-3차년도 자료를 활용, 발달 영역 별 중다회귀방법으로 분석한 결과는 다음과 같다. 첫째, 어린이집의 이용이 영아의 운동기능 및 언어, 사회성의 모든 발달영역에 미치는 영향이 모두 긍정적으로 작용하는 것으로 나타났다. 둘째, 어린이집 이용 시기에 따라서 각 발달 영역의 수준이 차이를 보였으며, 마지막으로 어린이집 인증여부는 영아의 사회성 발달 측면에서만 그 차이가 나타났다. 종합해 볼 때, 어린이집 이용이 영아의 발달 수준에 긍정적이라고 하더라도 어린이 평가인증 강화와 같은 서비스 질 제고 방안 역시 여전히 필요해 보인다.

• Child Health Nursing Research
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• 제17권1호
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• pp.39-47
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• 2011

Purpose: This study was done to determine whether a parenting education program using responsive teaching strategies is effective for parenting stress, maternal interactional behavior and behavioral problems in children at risk for Attention Deficit Hyperactivity Disorder (ADHD). Methods: Participants in this study were 17 elementary school children and their parents. The ADHD risk group was determined by scores on the Korean-ADHD Rating Scale. The parenting education program was developed based on Dr. Mahoney's responsive teaching curriculum. Mothers participated in the program once a week for 8 sessions. Treatment outcome was evaluated using the Korean version of the Child Behavior CheckList (K-CBCL), parenting stress, maternal interactional behavior, and child pivotal developmental behavior. Data were analyzed using Wilcoxon signed rank test, Mann-Whitney test, and regression analysis. Results: After the parenting education program, no significant difference in parenting stress (Z=-1.00, p=.320) was found, but there was a significant decrease in the child's internal behavior problems (Z=-2.05, p=.040), and also a significant improvement in maternal interactional behavior and a significant difference in child pivotal developmental behavior (Z=-2.67, p=.008). Conclusion: The results indicate that parenting education programs based on responsive teaching strategies are effective and that application of a program is recommended to prevent behavioral problems and improve maternal child interaction for children at risk for ADHD.

• 보건행정학회지
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• 제31권4호
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• pp.518-530
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• 2021

Background: This study examined the effect of having a usual source of care on the degree of patient-centered communication among persons with disability. The role of the usual source of care has been emphasized to improve patient experience, especially for patients with complex health conditions. Methods: This study used the 2017-2018 Korean Health Panel data, and the final study observations were 22,475 (20,806 people without disability and 1,669 people with disability). We applied generalized estimating equation model to show the effect of having a usual source of care on patient-centered communication, and subgroup analysis considering the types and severity of disabilities. Results: Persons who have disabilities, compared with ones without it, significantly had more usual sources of care (32.4% vs. 24.6%). By type of disability, persons with mental (51.4%), internal organ (43.8%), visual (37%), and physical disabilities (31.6%) had more usual sources of care than hearing/speech (26.6%), and developmental disabilities (18.6%). The average score of patient-centered communication was higher among who had a usual sources of care (3.2 vs. 2.7), and the regression analysis showed that having a usual sources of care was positively associated with higher patient-centered communication score (𝛽=0.476, p<0.05). However, the positive effects of usual sources of care was not observed among persons with severe hearing/speech, developmental, and mental disabilities. Conclusion: This study showed that role of patient-centered communication was limited in persons with severe hearing/speech disabilities, developmental, and mental disabilities. The education programs and supports are needed to improve communication skills between medical staff and persons with specific types of disabilities.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제22권3호
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• pp.141-148
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• 2011

Pbjective : A significant proportion of children with autism spectrum disorders (ASD) have regression characterized by loss of previously acquired skills. The purpose of this study was to compare demographic, clinical characteristics and autism-related symptomatology of the children who have regression with children who don't have regression. Methods : The subjects with ASD and their unaffected siblings (SIB) were recruited from the Korean Autism Genetic Study Consortium. Typically developing children (TC) were volunteered from community. The subjects were administered the Korean version of Autism Diagnostic Interview-Revised (K-ADI-R) and the Korean version of Autism Diagnostic Observation Schedule (K-ADOS) to diagnose or exclude ASD. Regression was defined on the basis of K-ADI-R data. The Korean version of Vineland Adaptive Behavior Scale (K-VABS), Aberrant Behavior Checklist (K-ABC) and Social Responsiveness Scale (K-SRS) were obtained from their parents. Results : Regression occurred in 8.33% (n=14) of children with ASD (n=168). Any SIB (n=166) and TC (n=53) did not experience regression. Regression was associated with lower IQ and lower score of K-VABS. There was no difference in autism symptom severity and K-ABC, K-SRS scores, between children with ASD who experienced regression and who did not. Conclusion : Regression seems to be a distinctive feature of ASD. Regression is associated with cognitive and more general functions, rather than symptoms specific to autism.

• 산업융합연구
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• 제20권3호
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• pp.73-79
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• 2022

본 연구는 발달장애 아동의 심리운동센터 이용 부모의 선택 동기, 만족도, 재이용 의사의 관계를 검증하고자 하였다. 이를 위해 수도권 소재의 사설 심리운동센터에서 심리운동프로그램에 참여하고 있는 아동의 부모를 대상으로 설문조사를 실시하였다. 수집된 자료는 188부이며, 서술통계, 요인분석과 신뢰도분석 그리고 상관분석과 회귀분석을 통하여 인과관계를 검증하였다. 분석 결과 다음과 같은 결론을 얻었다. 첫째, 발달장애 아동의 심리운동 참여에 대한 부모님의 선택동기와 만족도 관계에서 하위요인인 이용만족은 즐거움과 사교요인, 성과만족에서는 사교, 건강과 체력요인에서 유의미한 영향력을 나타내었다. 둘째, 선택 동기와 재이용 의사의 관계에서 즐거움, 기술발달 및 성취감, 사교요인에서 유의미한 영향력을 나타내었다. 셋째, 만족도와 재이용 의사의 관계에서 이용만족과 성과만족 요인에서 재이용 의사를 유의미하게 설명하였다. 향후 심리운동센터 재이용률을 높이기 위해 본 연구의 측정변인 외에 추가적인 분석이 필요하다고 판단된다.