• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.15-15
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• 2001

We recently demonstrated that satellite regions exhibit an aberrant DNA methylation in cloned bovine embryos. Here, we examined, using bisulfite -sequencing technology, whether the inefficient demethylation of cloned donor genomes could be rescued by the presence of oocytic nuclei. Both AciI digestion and sequencing analyses showed that satellite sequence was demethylated more efficiently in cloned tetraploid blastocysts than in diploid clones. When methyl -CpG density (the number of methyl-CpG sites per string) was scored, a significant decrease was observed In tetraploids (P<0.001). These results suggest that unknown mechanisms provided by oocytic nuclei could assist the demethylation of satellite sequences in tetraploid clones.

• 한국콘텐츠학회논문지
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• 제20권8호
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• pp.466-479
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• 2020

본 연구는 사회적 농업에서 발달장애인의 일자리 가능성을 모색하여 창출방안을 도출하고자 하였다. 이를 위해 해외 사회적 농업 활동 중에서 발달장애인 대상 사례를 참조하였다. 그리고 국내의 사회적 농장 다섯 곳을 방문하여 관찰하고, 담당자를 인터뷰하였다. 연구내용은 발달장애인의 일자리로서 사회적 농업의 의미와 가능성을 파악하고 사회적 농업에서 지속가능한 발달장애인 일자리 창출방안을 탐색하였다. 연구결과, 국내의 사회적 농업은 초기단계 있으며, 발달장애인에 대한 치유와 돌봄 중심의 농업체험 중심의 활동이 대부분이었다. 향후 지속적인 농업교육과 활동을 통해서 발달장애인에게 적합한 농업 일자리로서의 가능성이 충분하다는 결론을 도출하였다. 이러한 결과를 바탕으로 본 연구에서는 사회적 농업 분야에서 발달장애인 일자리 창출모형을 제안하였다. 본 연구에서 제시하는 일자리 창출모형은 크게 치유중심의 체험형, 돌봄 중심의 보호작업형, 사회적 일자리 모형으로 구분하였고, 사회적 일자리 모형에 스마트 팜 모형과 식물공장 모형을 추가하였다.

• 한국콘텐츠학회논문지
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• 제10권11호
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• pp.275-284
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• 2010

본 연구는 3-5세 아동을 대상으로 발달선별검사인 K-DDSTII, K-ASQ을 실시하여 발달지연 결과를 가진 하부검사와 가정환경 간의 관련성을 알아보았다. 연구결과, K-DDST에서는 가족환경 중 아버지의 나이와 개인사회성, 어머니의 나이와 교육력이 대동작과 통계적으로 차이가 있었다. 또한 K-ASQ에서는 의사소통과 성별, 출생순서와 부모의 교육력에서 통계적으로 유의한 차이가 있었으며, 문제해결과 출생순서, 그리고 하부검사 중 하나이상의 발달지연이 있는 경우는 성별과 출생순서와 유의미한 통계적 차이가 있었다. 본 연구결과 발달선별 검사 시에 가족환경에 관한 조사가 병행된다면 아동 발달에 대한 선별검사의 효율성을 높이는 방안의 하나일 수 있음을 제시 할 수 있을 것이다.

• 한국컴퓨터정보학회논문지
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• 제25권11호
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• pp.255-262
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• 2020

본 연구는 스마트폰 사용이 활성화된 사회 환경 속에서 발달장애 학생이 스마트폰 애플리케이션을 사회 실생활 도구로 효과적으로 활용할 수 있는 방안에 관해 '발달장애 학생, 정보사회, 스마트폰 사용, 애플리케이션 활용' 주제 관련 선행연구 문헌들을 검토하여 다음과 같이 살펴보았다. 첫째, 정보사회를 살아가는 발달장애 학생의 스마트폰을 활용한 사회통합 능력 향상 지원 방안을 기술한다. 둘째, 발달장애 학생의 사회 실생활을 지원해 줄 수 있는 스마트폰 애플리케이션 유형을 기술한다. 셋째, 발달장애 학생의 사회 실생활 적응을 위한 스마트폰 애플리케이션 요구 영역을 기술한다. 이 연구 결과는 발달장애 학생이 정보사회에서 스마트폰의 다양한 기능을 익히고 배워 이용함으로써 주변 사람들과 상호교류하여 의사소통능력을 향상시키고 직업재활과 사회통합에 도움이 되도록 적절한 애플리케이션을 개발하여 교육적으로 지원, 제공하는 기초자료로 활용될 수 있을 것이다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1996년도 식물학심포지움 식물호르몬과 신호전달
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• pp.68-78
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• 1996

Two types of immunoloigcal probes, anti-ABBP Abs, have been developed. The purified ABBP from ABA-C1-BSA-sepharose 4B column was identified by PAGE and appeared in one band of about 56KD, as well as showed a specific binding ability and a high affinity for ABA (Kd2.0$\times$10-9 mol/L). Unexpectedly, the existence of rRNA with a length of around 300 nucleotides could be found, when the ABBP was digested with proteinase K and identified by eletrophorsis on an agarose gel (1%). As a result, about 120 cDNA clones coding maize 17s RNA and only one cDNA clone coding ABBP (24cDNA) were obtained from 200,000 seperated phage plaques by the anti-ABBP pAbs. 24cDNA had 1075bp and contained an open reading frame coding 254 amino acids. The anti-idiotypic Ab raised against an ABA MAb showed the ability of either mimicking ABA or competing with ABA. The localization of ABBPs in plant cell was investigated.

• 지역사회간호학회지
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• 제12권1호
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• pp.49-59
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• 2001

Purpose: This study was intended to develop the framework of public health center based 'Child growth and developmental clinic' and analyze the efficiency of services. This was the part of Mother-Child Health Guidance Project by the Public Health Center in Seoul. The purposes of this project were to improve children's health and development with early detection of developmental delay by screening test, and to assist and guide the parent for child care and rearing, so that children can reach their maximum potential. Method: Framework and standards for operation of 'Child growth and development clinic' were developed. We selected screening tool. developed parent education materials, and educated the personnel of public health center. Result: 243 children were enrolled for developmental screening, and we detect the children with developmental delay and referred them for further evaluation. The proportion of developmental delayed children was approximately 2% of the enrolled children. Also, we analyzed the characteristics of enrolled children, and their parental needs and attitude on child rearing. Some problems in operation of developmental clinic were discussed. Conclusion: Though we can found some limitations, we are confident that pubic health center can be charge of important role for improving child development for the majority. This study will be the basis of children's developmental clinic running by public health center.

• 한국콘텐츠학회논문지
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• 제21권11호
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• pp.711-722
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• 2021

본 연구의 목적은 발달장애인의 취업이 사회활동에 미치는 영향을 파악하여 발달장애인의 사회통합 활동 방안을 모색하는 것이다. 연구방법으로 391명의 발달장애인을 대상으로 취업 여부와, 사회활동에 대한 설문조사 자료를 통해 교차분석과 상관분석, 그리고 로지스틱 회귀분석을 실시하였다. 분석결과 첫째, 발달장애인의 취업은 사회활동에 유의한 것으로 나타났다. 즉 취업한 발달장애인의 사회활동 참여는 79.7% 미취업 상태는 39.3%로 나타났다. 둘째, 개인적 특성을 통제변인으로 신중하게 검증한 발달장애인의 취업은 사회활동에 유의한 영향을 미치는 것으로 5.2배 증가하였다. 연구결과로 볼 때 발달장애인의 사회활동 참여를 위해서는 발달장애인의 적극적인 취업지원과 취업에 대한 정책적 노력이 요구됨을 알 수 있다. 마지막으로 지원고용과 장애인 표준사업장 확대, 직무지도원 제도 도입과 관련 기관과의 네트워크 구축을 제언하였다.

• Child Health Nursing Research
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• 제13권2호
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• pp.222-229
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• 2007

Purpose: This study was done to evaluate the validity of the Korean - Child Development Review in screening children with developmental risks. Method: The participants in the study were 172 children aged 6-75 months old and their parents. Data were collected by questionnaire or interview. Korean Denver II and Bayley test were administered with K-CDR for validation of children who were referred for developmental assessment at D University Hospital or public health center. The data were analyzed using correlation, $X^2$ test, and cross tab analysis. Results: There was a significant relationship between the K-CDR and Korean Denver II. The correlation coefficients were .42 to .61 by developmental sector. The sensitivity and specificity of K- CDR were .84 and .66 respectively when "abnormal" with MDI of Bayley test defined as lower than 85 the score which was used as a diagnosis of developmental delay. The responses to 6 questions for parents showed significant differences according to 3 groups by developmental state. Parents of children in the developmental disability group more frequently checked problems from the 26 problem checklist about their children's development and behavior. Conclusion: The results show that K-CDR is valid and has good sensitivity and moderate specificity in screening developmental delay.

• 한국임상보건과학회지
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• 제2권1호
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• pp.47-57
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• 2014

Purpose. The purpose of this study is to investigate effects of group play therapy program on improvement of social skills of children with developmental disabilities. Methods. With 4-year children with developmental disabilities with developmental problems, group play therapy program with 16 sections, 40minutes for each section for 16 weeks was conducted, and by using Korean infant social skills test (K-SSRSP), social skills index (SSQ) between before and after applying group play therapy program on children with developmental disabilities were compared. Results. When group play therapy are applied on children with developmental disabilities, it was found that there was improvement of social skills including problem solving, emotional expression, sense of order and self-confidence. Conclusions. According to the result of this study, if more specific studies regarding group play therapy program are conducted and they can reflect on other integrated programs, then it is expected that there will be greater synergy effects on improving not only children with developmental disabilities but also social skills of them.

• Toxicological Research
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• 제29권4호
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• pp.221-227
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• 2013

Embryonic stem (ES) cells have potential for use in evaluation of developmental toxicity because they are generated in large numbers and differentiate into three germ layers following formation of embryoid bodies (EBs). In earlier study, embryonic stem cell test (EST) was established for assessment of the embryotoxic potential of compounds. Using EBs indicating the onset of differentiation of mouse ES cells, many toxicologists have refined the developmental toxicity of a variety of compounds. However, due to some limitation of the EST method resulting from species-specific differences between humans and mouse, it is an incomplete approach. In this regard, we examined the effects of several developmental toxic chemicals on formation of EBs using human ES cells. Although human ES cells are fastidious in culture and differentiation, we concluded that the relevancy of our experimental method is more accurate than that of EST using mouse ES cells. These types of studies could extend our understanding of how human ES cells could be used for monitoring developmental toxicity and its relevance in relation to its differentiation progress. In addition, this concept will be used as a model system for screening for developmental toxicity of various chemicals. This article might update new information about the usage of embryonic stem cells in the context of their possible ability in the toxicological fields.