• 한국융합학회논문지
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• 제8권12호
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• pp.215-220
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• 2017

도꼬마리 (X. strumarium )는 국화과에 속하며, '창이자'라는 한약명으로 사용되는 다년생 식물이다. 열매부위와 지상부위의 추출물을 이용하여 융합 식물활성작용을 관찰하였다. 산화적 스트레스에 대항하는 실험법으로는 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), ABTS assay를 사용하였다. 도꼬마리의 추출물은 단일분리가 아닌 ethanol, petroleum ether, 그리고 ethyl acetate로 추출하였다. 산화적 스트레스연구에 사용되는 radical scavenging 능력을 확인하기 위하여 DPPH radical and ABTS를 투여하였다. 본 연구에서 도꼬마리는 천연유래 항산화 효과를 보였다.

• Ocean and Polar Research
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• 제34권4호
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• pp.445-451
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• 2012

The EtOH extracts of red seaweeds (Symphyocladia latiuscula, Chondrus ocellatus and Carpopeltis affinis) and solvent partitioned fractions were investigated for their 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effects and the total phenolic contents were correlated with ABTS and DPPH radical scavenging activities. The EtOH extracts and their solvent partitioned fractions exhibited strong ABTS and DPPH radical scavenging activities. Among the solvent partitioned fractions obtained from n-Hexane (HX), methylenchloride (MC), ethylacetate (EA), and buthanol (BuOH), the HX fraction from C. affinis showed higher radical scavenging activities than other fractions. Total phenolic contents showed significant correlation ($r^2$ = 0.709) with ABTS radical scavenging activity. The results of this study suggest that the strong radical scavenging activity of HX fraction from C. affinis is a promising natural antioxidant for healthcare products.

• Fisheries and Aquatic Sciences
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• 제25권6호
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• pp.335-349
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• 2022

To optimize the hydrolysis conditions in the production of antioxidant hydrolysates from tuna cooking juice concentrate (TC) to maximize the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, TC containing 48.91% protein was hydrolyzed with Alcalase 2.4 L, and response surface methodology (RSM) was applied. The optimum hydrolysis conditions included a 2.2% (w/v) Alcalase concentration and 281 min hydrolysis time, resulting in the highest DPPH radical scavenging activity of 66.49% (0.98 µmol Trolox/mg protein). The analysis of variance for RSM showed that hydrolysis time was an important factor that significantly affected the process (p < 0.05). The effects of different drying methods (freeze drying, hot air drying, and vacuum drying) on the DPPH radical scavenging activity and amino acid (AA) profiles of TC hydrolysate (TCH) were evaluated. Vacuum-dried TCH (VD) exhibited an increase in DPPH radical scavenging activity of 81.28% (1.20 µmol Trolox/mg protein). The VD samples were further fractionated by ultrafiltration. The AA profiles and antioxidant activities in terms of the DPPH radical scavenging activity, 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power, and ferrous ion chelating activity were investigated. Glutamic acid, glycine, arginine, and cysteine were the major AAs found in the TCH fractions. The highest DPPH radical scavenging activity was found in the VD-1 fraction (< 5 kDa). The VD-3 fraction (> 10 kDa) exhibited the highest ABTS radical scavenging activity and ferric reducing antioxidant power. The ferrous ion chelating activity was the highest in VD-1 and VD-2 (5 to 10 kDa). In conclusion, this study provided the optimal conditions to obtain high antioxidant activities through TCH production, and these conditions could provide a basis for the future application of TCH as a functional food ingredient.

• 한국환경과학회지
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• 제31권8호
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• pp.715-725
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• 2022

Lactic Acid Bacteria (LAB) are among the representative probiotics that have been used for a long time in fermented food. Although there are many studies on detecting the radical scavenging activity of LAB, few studies have been conducted on the environmental factors that improve scavenging activity. This study investigated the environmental factors affecting the DPPH radical scavenging and various antioxidant activities of Kimchi-derived Lactobacillus plantarum K-21 with antihypertensive and radical scavenging activities. The optimal conditions for scavenging DPPH radicals were glucose 2%, bactopeptone 0.5%, Tween 80 0.05%, L-cysteine 0.05%, and an initial pH 6.5 at 35℃. Under optimal conditions, the DPPH radical scavenging activity was 94.8±2.2%, which was 1.5 times higher than that of the basic medium. In addition, L. plantarum K-21 had other antioxidant activities; ABTS radical scavenging (93.6±1.5%), hydroxyl radical scavenging (8.5±0.9%), metal chelating (65.9±0.5%), NO scavenging (53.1±19%), SOD-like (25.1±1.5%), and reducing power (11.7±1.4%) activities were detected. Therefore, L. plantarum K-21 may act not only as a starter for lactic acid-fermented foods with improved functionality but also as a drug for various diseases caused by oxidative stress.

• 농약과학회지
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• 제7권2호
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• pp.92-99
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• 2003

96-well plate를 사용해서 DPPH free radical 소거활성의 고효율검정 (high throughput screening)방법을 확립하였고, 이 방법을 이용하여 107개의 식물특정 효소저해제와 다양한 식물 추출물의 항산화활성을 조사하였다. DPPH free radical 소거활성 측정 측정을 위한 적정 시험조건은 총 반응액이 $250{\mu}L$일 경우 $100{\mu}M$의 DPPH(pH 7.8), 20 분의 반응시간이었고, 이 조건하에서 ascorbate와 a-tocopherol 은 농도 의존적인 항산화활성을 나타내었다. 107개의 식물 특정효소저해제 중에서 11개의 화학물질이 $100{\mu}M$ 농도에서 70% 이상의 항산화활성이 있었는데, 특히 ampicillin과 gallic acid는 각각 90.2% 와 92.6% 의 나타냈다. 또한 100개의 식물 추출물은 $50{\mu}g/mL$ 농도에서 70% 이상의 활성을 보이는 추출물이 11개이었는데, 그 중에서 AT-407의 활성이 90.1%로 가장 높게 나타났다. 따라서 96-well plate를 이용한 DPPH free ra 이때 소거활성 측정방법은 여러가지 합성물질이나 다양한 천연물질에 대하여 보다 간편하고 신속하게 항산화활성을 측정할 수 있을 것으로 사료되었다.

• 생약학회지
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• 제35권4호통권139호
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• pp.338-345
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• 2004

Scavenging effects of DPPH radical and hydroxyl radical, inhibition of linoleic acid oxidation, inhibition of NO synthesis and iNOS expression were tested with extracts and chromatographic subfractions of Canavalia lineata obtained at Jeju island. Chloroform extract and its subfractions gave moderate effects on scavenging DPPH radical and hydroxyl radical, They also inhibited linoleic acid oxidation, and NO synthesis. Inhibition of NO synthesis resulted from the repression of iNOS gene expression. Ethyl acetate extract and its subfractions showed excellent effects on scavenging DPPH radical and hydroxyl radical, while they were cytotoxic.

• 한국산림과학회지
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• 제102권4호
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• pp.566-570
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• 2013

정금나무의 기능성 품종을 육성하기 위하여 정금나무 선발 5개체의 열매에 대한 항산화 활성을 측정하였으며, 항산화 성분인 총 페놀 함량을 측정하였다. 총 페놀 함량은 gallic acid를 기준물질로 하여 분석한 결과 15.8~19.9 mg/g 범위로 전체 평균 17.9 mg/g을 나타냈으며, DPPH free radical 소거활성은 400 ppm의 농도에서 69.1~91.4% 범위로 전체평균 84.2%를 나타내었다. 환원력은 0.56~0.96 범위로 전체 평균 0.79를 나타내었다. 상관분석 결과 DPPH free radical 소거활성과 환원력에서 상관계수가 0.969로 매우 높은 정의 상관관계를 나타내었다.

• Ocean and Polar Research
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• 제26권1호
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• pp.59-64
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• 2004

As a part of our search for novel antioxidants from the seaweeds, we have investigated radical scavenging effect for their crude extracts using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1), a peroxynitrite-generating species in vitro. Thirty-four seaweeds were screened for $ONOO^-$ and DPPH radical scavenging activities. A potent inhibitory effect against peroxynitrite generated by SIN-1 at $5{\mu}g/ml$ of methanol extracts was observed in order of Ishige okamurae(95.3%), Sargassum hemiphyllum(90.2%), Symphyocladia latiuscula(89.6%), Porphyra suborbiculata(86.7%), and Gelidium amamsii(85.9%), Also, a significant scavenging effect against direct authentic peroxynitrite was revekaled for methanol extracts of Ishige okamurae(66.2%) and Sargassum hemiphyllum(55.2%) and the acetone/methylene chloride(1:1) extract of Gigatina tenella (61.0%). In our measurement for evaluating the capacity to scavenge the stable free radical of DPPH, acetone/methylene chloride(1:1) extracts of Symphyocladia latiuscula, Gloiopeltis furcata, and Sargassum thunbergii and the methanol extract of Sargassum sp. showed an inhibitory potency of 85.8%, 82.8%, 74.1%, and 64.0%, respectively.

• 생명과학회지
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• 제11권6호
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• pp.509-516
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• 2001

까마중의 부위별 DPPH free radical 소거활성은 뿌리에서 가장 높았고, 줄기, 전부위, 열매 잎 그리고 꽃 순으로 높았다. 추출방법간의 DPPH free radical 소거활성은 80%methanol에 침지하여 추출한것보다는 $80^{\circ}C$에서 80% meth-anol에 흰류추출한것이 높았다. Sephades LH-20 column chromatography에 의하여 분획된 L6의 DPPH free radical 소거활성은 ethy acetate 분획 추출물보다 6.7배 높았다. 분획물내의주요물질은 2, 6-methano-3-benzazocin-11-ol, 2[1h]-pyridinethione 및 2-hydroxy-5 methylbenzaldehyde로 phenolic 화 물이었다. 줄기와 뿌리의 POD및 SOD 활성은 타 부위에 비하여 높은 활성을 나타내었고, 생육기간에는 파종 후 60일후에서 활성이 가장 높았다. PODdmlehddnl gth 패턴은 뿌리에서 10개의 band로 가장 많았고 그중 8개의 band가 식물체의 부위별로 차이가 있어Tdmaul, SOD의 동위효소 패턴은 잎에 5개의 band로 가장 많았고 그중 2개의 band가 식물체의 부위별로 차이가 있었다.이가 있었다.

• 한국자원식물학회지
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• 제26권3호
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• pp.347-354
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• 2013

Apple and pear are popular fruits consumed in Korea and are common fruit in daily diet. In order to compare the antioxidant activity of the apple and pear peels, total polyphenol contents, total flavonoid contents, $ABTS^+$ free radical scavenging activity, and DPPH free radical scavenging activity were measured from hot water, ethanol, and methanol extracts of the two fruit peels. The total polyphenol and flavonoid contents were highest in 95% methanol extracts of the apple peelsand 70% ethanol extract of the pear peels, respectively. Total polyphenol contents of the pear peels were higher than that of apple peels, and total flavonoid contents of the apple peels were higher than that of pear peels. The apple and pear peels had the highest $ABTS^{+{\cdot}}$ and DPPH free radical scavenging activity in 95% methanol extracts and 70% ethanol extracts, respectively. $ABTS^{+{\cdot}}$ and DPPH free radical scavenging activity of pear peels was higher than that of apple peels, and the DPPH free radical scavenging activity of apple and pear peels were detected in hot water, 95% methanol, and 70% ethanol extracts, respectively. Ascorbic acid, a synthetic antioxidant used as positive control, had significantly higher scavenging activity than the apple and pear peels. In conclusion, the apple and pear peelshave great potential as natural antioxidants. Therefore, above results should be considered to provide the possibility for the development of high functional antioxidants.