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Analysis of Total Phenolic Content and Antioxidant Activity from Fruits of Vaccinium oldhamii Miq.

정금나무 열매의 총 페놀 함량 및 항산화활성 분석

  • Kim, Hyeusoo (Division of Special Purpose Trees, Korea Forest Research Institute) ;
  • Kim, Moon Sup (Division of Special Purpose Trees, Korea Forest Research Institute) ;
  • Kim, Sea Hyun (Division of Special Purpose Trees, Korea Forest Research Institute) ;
  • Yun, Kyeong Won (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Song, Jeong Ho (Division of Special Purpose Trees, Korea Forest Research Institute)
  • 김혜수 (국립산림과학원 특용자원연구과) ;
  • 김문섭 (국립산림과학원 특용자원연구과) ;
  • 김세현 (국립산림과학원 특용자원연구과) ;
  • 윤경원 (국립순천대학교 한약자원학과) ;
  • 송정호 (국립산림과학원 특용자원연구과)
  • Received : 2013.08.14
  • Accepted : 2013.09.27
  • Published : 2013.12.31

Abstract

Vaccinium oldhamii Miq. is a Korean native tree, which is deciduous and shrub tree with broad leaf. To develop superior cultivars for edible or medicinal purposes, we evaluated total phenolic content and antioxidant activity. Total phenolic content was measured by folin-denis method and antioxidant activity was measured by DPPH free radical scavenging activity (DPPH method) and reducing power (potassium ferricyanide method). Total phenolic content, DPPH free radical scavenging activity (at 400 ppm) and reducing power (at 700 ppm) were 15.8~19.9 mg/g, 69.1~91.4% and 0.56~0.96, respectively. A linear correlation was shown between DPPH-radical scavenging activity and reducing power (r=0969).

정금나무의 기능성 품종을 육성하기 위하여 정금나무 선발 5개체의 열매에 대한 항산화 활성을 측정하였으며, 항산화 성분인 총 페놀 함량을 측정하였다. 총 페놀 함량은 gallic acid를 기준물질로 하여 분석한 결과 15.8~19.9 mg/g 범위로 전체 평균 17.9 mg/g을 나타냈으며, DPPH free radical 소거활성은 400 ppm의 농도에서 69.1~91.4% 범위로 전체평균 84.2%를 나타내었다. 환원력은 0.56~0.96 범위로 전체 평균 0.79를 나타내었다. 상관분석 결과 DPPH free radical 소거활성과 환원력에서 상관계수가 0.969로 매우 높은 정의 상관관계를 나타내었다.

Keywords

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