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Physicochemical Characteristic of the Silkworm Sericin Cocoon (세리신잠견의 이화학적 특성)

  • 김수연;손해룡;배도규;김정호
    • Journal of Sericultural and Entomological Science
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    • v.45 no.1
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    • pp.10-17
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    • 2003
  • This study was carried out to analyze physicochemical characteristics of sericin cocoon from silkworm, Bombyx mori. The degumming loss increased with increasing treatment time up to 2 hr, and temperature up to 130$^{\circ}C$. At 130$^{\circ}C$, degumming loss of Nd-s jam and Nd$\^$H/ jam were 100% while that of Baegok jam was 24%. Nd-s jam and Baegok jam ha high glycine content of 29.1∼46.3 mol% where as Nd$\^$H/ jam had high serine content of 32.6 mol%. Thermal denaturation temperatures were found at 218$^{\circ}C$ for Nd-s jam, 216$^{\circ}C$ for Nd$\^$H/ jam, and 218$^{\circ}C$ for Baegok jam. Before degumming, crystallinities obtained by FT-IR analysis were 44.3, 43.7, and 59.9% for Nd-s jam, Nd$\^$H/ jam, and Baegok jam respectively. After degumming, crystallinity increased to 61.8% for Baegok jam. Before degumming, crystallinitics obtained from XRD were 35.9, 33.5, and 47.2%, for Nd-s jam, Nd$\^$H/ jam, and Baegok jam. After degumming, crystallinity increased to 49.8% for Baegok jam. The molecular weight of Nd$\^$H/ jam were 9,417 in 1 hr, 3,744 in 2 hr, 4,944 in hr, and 3,910 in 6 hr.

CONVOLUTION SUMS OF ODD AND EVEN DIVISOR FUNCTIONS

  • Kim, Daeyeoul
    • Honam Mathematical Journal
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    • v.35 no.3
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    • pp.445-506
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    • 2013
  • Let ${\sigma}_s(N)$ denote the sum of the s-th power of the positive divisors of N and ${\sigma}_{s,r}(N;m)={\sum_{d{\mid}N\\d{\equiv}r\;mod\;m}}\;d^s$ with $N,m,r,s,d{\in}\mathbb{Z}$, $d,s$ > 0 and $r{\geq}0$. In a celebrated paper [33], Ramanuja proved $\sum_{k=1}^{N-1}{\sigma}_1(k){\sigma}_1(N-k)=\frac{5}{12}{\sigma}_3(N)+\frac{1}{12}{\sigma}_1(N)-\frac{6}{12}N{\sigma}_1(N)$ using elementary arguments. The coefficients' relation in this identity ($\frac{5}{12}+\frac{1}{12}-\frac{6}{12}=0$) motivated us to write this article. In this article, we found the convolution sums $\sum_{k<N/m}{\sigma}_{1,i}(dk;2){\sigma}_{1,j}(N-mk;2)$ for odd and even divisor functions with $i,j=0,1$, $m=1,2,4$, and $d{\mid}m$. If N is an odd positive integer, $i,j=0,1$, $m=1,2,4$, $s=0,1,2$, and $d{\mid}m{\mid}2^s$, then there exist $u,a,b,c{\in}\mathbb{Z}$ satisfying $\sum_{k& lt;2^sN/m}{\sigma}_{1,i}(dk;2){\sigma}_{1,j}(2^sN-mk;2)=\frac{1}{u}[a{\sigma}_3(N)+bN{\sigma}_1(N)+c{\sigma}_1(N)]$ with $a+b+c=0$ and ($u,a,b,c$) = 1(Theorem 1.1). We also give an elementary problem (O) and solve special cases of them in (O) (Corollary 3.27).

Comparison of Live Performance and Meat Quality Parameter of Cross Bred (Korean Native Black Pig and Landrace) Pigs with Different Coat Colors

  • Hur, S.J.;Jeong, T.C.;Kim, G.D.;Jeong, J.Y.;Cho, I.C.;Lim, H.T.;Kim, B.W.;Joo, S.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.7
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    • pp.1047-1053
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    • 2013
  • Five hundred and forty crossbred (Korean native black pig${\times}$Landrace) F2 were selected at a commercial pig farm and then divided into six different coat color groups: (A: Black, B: White, C: Red, D: White spot in black, E: Black spot in white, F: Black spot in red). Birth weight, 21st d weight, 140th d weight and carcass weight varied among the different coat color groups. D group (white spot in black coat) showed a significantly higher body weight at each weigh (birth weight, 140th d weight and carcass weight) than did the other groups, whereas the C group (red coat color) showed a significantly lower body weight at finishing stage (140th d weight and carcass weight) compared to other groups. Meat quality characteristics, shear force, cooking loss and meat color were not significantly different among the different coat color groups, whereas drip loss was significantly higher in F than in other groups. Most blood characteristics were not significantly different among the different groups, except for the red blood cells.

Comparison of Properties of Natural Ca-Montmorillonite and its Al-pillared Montmorillonites (천연 Ca-몬모릴로나이트와 이로부터 합성된 Al-가교몬모릴로나이트의 특성 비교연구)

  • 이정현;김수진
    • Journal of the Mineralogical Society of Korea
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    • v.15 no.4
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    • pp.273-282
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    • 2002
  • Al-pillared montmorillonite was synthesized from Na-saturated montmorillonite which was prepared by ionic substitution from Ca-montmorillonite of the Jabut mine, Gyeongiu City d(001), surface areas, and dehydration and ionic substitution properties have been compared for both Ca-montmorillonite and Al-pillared montmorillonite. d(001) spacings of Ca-montmorillonite and Al-pillared montmorillonite were 15.1 $\AA$ and $18.3\AA$, respectively. Dehydration took place before $350 ^{\circ}C$ in Ca-montmorillonite, whereas linealy up to $550^{\circ}C$ in Al-pillared montmorillonite. BET surface areas are 5~6 times larger in Al-pillared montmorillonite ($192 \m^2$/g) than Camontmorillonite. Ca-montmorilonite shows high selectivity for $Na^{+}$ /, whereas Al-pillared montmorillonite for $Ca^{2+}$ . The former shows decreasing d(001) spacing with increasing substitution of $Na^{+}$ and irregular interstratified structure at high substitution of $Ca^{2+}$ /, whereas the latter shows linear decreasing pattern in d(001) spacing with increasing $Ca^{ 2+}$.

Expression of the EPO-like Domains of Human Thrombopoietin in Escherichia coli

  • Koh, Yeo-Wook;Koo, Tai-Young;Ju, Sang-Myoung;Kwon, Chang-Hyuk;Chung, Joo-Young;Park, Myung-Hwan;Yang, Jai-Myung;Park, Seung-Kook
    • Journal of Microbiology and Biotechnology
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    • v.8 no.6
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    • pp.553-559
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    • 1998
  • cDNA of human thrombopoietin (hTPO) amplified by polymerase chain reaction from a cDNA library of human fetal liver was cloned. EPO-like domains ($hTPO_{153} \;or\; hTPO_{l63})\; of\; hTPO(hTPO_{332}$) were expressed in Escherichin coli using several kinds of expression systems, such as ompA secretion, thioredoxin fusion, and the $P_L$ and T7 expression systems. To obtain $hTPO_{153}$ in soluble form, $hTPO_{153}$ cDNA was fused in-frame behind the gene encoding ompA signal sequence and thioredoxin protein. When fused with either of the genes, $hTPO_{153}$ was not expressed to the detectable level. However, a high level expression of the EPO-like domain of hTPO was obtained using the PL and T7 expression system. $hTPO_{153} \;or\; hTPO_{l63} cDNA were subcloned into the pLex and pET-28a(+) vectors under the control of the inducible$ P_L\;T_7$ promoter, respectively. Proteins expressed using pl.ex vector and pET-28a(+) detected in insoluble forms with an expression level of about 14% and 9% of total cellular proteins, respectively, and the level of expression was rapidly diminished in 2 h after the maximum level of expression was reached.

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New Model of In-situ Xenograft Lymphangiogenesis by a Human Colonic Adenocarcinoma Cell Line in Nude Mice

  • Sun, Jian-Jun;Jing, Wei;Ni, Yan-Yan;Yuan, Xiao-Jian;Zhou, Hai-Hua;Fan, Yue-Zu
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.6
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    • pp.2823-2828
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    • 2012
  • Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice. Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models. Results: Some microlymphatics with thin walls, large and irregular or collapsed cavities and increased LMVD, with strong positive of LYVE-1, D2-40 in immunohistochemistry, were observed, identical with the morphological characteristics of lymphatic vessels and capillaries. Expression of LYVE-1 and D2-40 proteins and mRNAs were significantly higher in xenograpfts in-situ than in the negative control group(both P<0.01). Moreover, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins and mRNAs were significantly higher in xenografts in-situ (both P<0.01), in conformity with the signal regulation of the VEGF-C,-D/VEGFR-3 axis of tumor lymphangiogenesis. Conclusions: In-situ xenografts of a human colonic adenocarcinoma cell line demonstrate tumor lymphangiogenesis. This novel in-situ animal model should be useful for further studying mechanisms of lymph node metastasis, drug intervention and anti-metastasis therapy in colorectal cancer.

Combined Treatment with 2-Deoxy-D-Glucose and Doxorubicin Enhances the in Vitro Efficiency of Breast Cancer Radiotherapy

  • Islamian, Jalil Pirayesh;Aghaee, Fahimeh;Farajollahi, Alireza;Baradaran, Behzad;Fazel, Mona
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.18
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    • pp.8431-8438
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    • 2016
  • Doxorubicin (DOX) was introduced as an effective chemotherapeutic for a wide range of cancers but with some severe side effects especially on myocardia. 2-Deoxy-D-glucose (2DG) enhances the damage caused by chemotherapeutics and ionizing radiation (IR) selectively in cancer cells. We have studied the effects of $1{\mu}M$ DOX and $500{\mu}M$ 2DG on radiation induced cell death, apoptosis and also on the expression levels of p53 and PTEN genes in T47D and SKBR3 breast cancer cells irradiated with 100, 150 and 200 cGy x-rays. DOX and 2DG treatments resulted in altered radiation-induced expression levels of p53 and PTEN genes in T47D as well as SKBR3 cells. In addition, the combination along with IR decreased the viability of both cell lines. The radiobiological parameter (D0) of T47D cells treated with 2DG/DOX and IR was 140 cGy compared to 160 cGy obtained with IR alone. The same parameters for SKBR3 cell lines were calculated as 120 and 140 cGy, respectively. The sensitivity enhancement ratios (SERs) for the combined chemo-radiotherapy on T47D and SKBR3 cell lines were 1.14 and 1.16, respectively. According to the obtained results, the combination treatment may use as an effective targeted treatment of breast cancer either by reducing the single modality treatment side effects.

Effect of Number and Location of Amine Groups on the Thermodynamic Parameters on the Acridine Derivatives to DNA

  • Kwon, Ji Hye;Park, Hee-Jin;Chitrapriya, Nataraj;Han, Sung Wook;Lee, Gil Jun;Lee, Dong Jin;Cho, Tae-Sub
    • Bulletin of the Korean Chemical Society
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    • v.34 no.3
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    • pp.810-814
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    • 2013
  • The thermodynamic parameters for the intercalative interaction of structurally related well known intercalators, 9-aminoacridine (9AA) and proflavine (PF) were determined by means of fluorescence quenching study. The fluorescence intensity of 9AA decreased upon intercalation to DNA, poly[$d(A-T)_2$] and poly[$d(G-C)_2$]. A van't Hoff plot was constructed from the temperature-dependence of slope of the ratio of the fluorophore in the absence and presence of a quencher molecule with respect to the quencher concentration, which is known as a Stern-Volmer plot. Consequently, the thermodynamic parameters, enthalpy and entropy change, for complex formation was calculated from the slope and y-intercept of the van't Hoff plot. The detailed thermodynamic profile has been elucidated the exothermic nature of complex formation. The complex formation of 9AA with DNA, poly[$d(A-T)_2$] and poly[$d(G-C)_2$] was energetically favorable with a similar negative Gibb's free energy. On the other hand, the entropy change appeared to be unfavorable for 9AA-poly[$d(G-C)_2$] complex formation, which was in contrast to that observed with native DNA and poly[$d(A-T)_2$] cases. The equilibrium constant for the intercalation of PF to poly[$d(G-C)_2$] was larger than that to DNA, and was the largest among sets tested despite the most unfavorable entropy change, which was compensated for by the largest favorable enthalpy. The favorable hydrogen bond contribution to the formation of the complexes was revealed from the analyzed thermodynamic data.

Effect of Chungsimyeonjatang on Myocardiac Cell Injury in Mouse Myoblast $C_2C_{12}$ Cells (Mouse 유래 $C_2C_{12}$세포주에서 청심연자탕(淸心蓮子湯)의 심근세포 손상 억제 효과)

  • Yoon, Hyeon-Deok;Shin, Oh-Chul;Shin, Yoo-Jeong;Kim, Seung-Mo;Park, Chi-Sang
    • The Journal of Internal Korean Medicine
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    • v.28 no.2
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    • pp.333-345
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    • 2007
  • Determination and differentiation of cells in the skeletal muscle lineage is positively regulated by cell-cell contact. differentiation proteins proposed to mediate this effect include both classical MyoD and MEF members : potential interactions between the promyogenic activities of these classes of protein, however, are unknown. We show here that MyoD and MEF, two promyogenic family members that determine to each other in a cis fashion, form ineraction with MyoD- and MEF. These proteins contain myosin heavy chains and are enriched at sites of cell-cell contact between myoblasts, Therefore, In differentiation of MyoD MEF from CST (Chungsimyeonjatang) interact dependently, suggesting that the interactions occur in a cis fashio : consistent with this conclusion, MyoD-mediated differentiation is required for myoblast to occur by CST. Inhibition in myoblasts of a MyoD by STP in its ability to associate with MEF interferes with differentiation as assessed by morphological and transcription level, suggesting that this interaction is functionally important in myogenesis. Also, some of the differentiation-mediated proteins that are required for myogenesis seem to be based on interdependent activities of promyogenic classical SMAD-subfamilly.

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Purification and Characterization of Ice Nucleating Proteins from Ice Nucleation-Active Bacteria (빙핵활성 세균으로부터 빙핵활성 단백질의 정제 및 특성)

  • Kim, Ki-Chung;Lee, Ung;Song, Dong-Up;Cho, Baik-Ho
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.99-108
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    • 1996
  • 3종의 빙핵세균 Peudomonas syringae 8401, Pseudomonas fuorescens 8701, Erwinia herbicola 8701의 세포 외막으로부터 아무런 변성제도 사용치 않고 sucrose density gradient centrifugation, Sephacryl gel filtration chromatography, DEAE-cellulose ion exchange chromatography, non-denaturing buffer를 이용한 PAGE, electroelution, SDS-PAGE를 통해 빙핵활성 단백질을 고도로 정제할 수 있었다. P. suringae와 P. fluorescens에서는 각각 3종류(155 kD, 75 kD, 50 kD)의 빙핵활성 단백질이, E. herbicola에서는 155 kD를 제외한 2종류(75 kD, 50 kD)의 빙핵활성 단백질은 이 연구를 통해 처음 밝혀진 것으로 , 지금까지 보고된 빙핵활성 단백질(150 KD 이상)보다는 훨씬 작은 것이다. 이는 빙핵활성을 나타내는 단백질의 기본단위는 이 실험의 결과만에 의하면 최대 50 kD임을 시사한다. 이들 단백질은 그 유래된 세균의 종류나 또는 단백질 분자량의 크기에 관계없이 모두 -5.5~7.5$^{\circ}C$에서 물을 동결시키는 높은 빙핵활성을 갖고 있었다. 이는 지금까지 보고된 어느 정제단백질의 빙핵활성보다 높은 것이다. 정제된 단백질의 빙핵활성은 trypsin 처리에 의해 상실되었고, pH6~8범위에서는 안정하였으며, pH5이하, pH9이상에서는 활성을 상실하였다. 보존온도에 대한 영향은 3$0^{\circ}C$이상이 되면 점차 활성이 감소하는 경향을 보이다 37$^{\circ}C$이상에서는 활성이 완전히 상실되었다. 금속이온으로서 Hg\ulcorner이온과 SDS에 의해 활성이 상실되었으나 phosphatidylinositol의 첨가에 의해서는 활성이 약간 증가(-1$^{\circ}C$)하였다.

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