The Journal of Internal Korean Medicine (대한한방내과학회지)
- Volume 28 Issue 2
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- Pages.333-345
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- 2007
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- 1226-9174(pISSN)
Effect of Chungsimyeonjatang on Myocardiac Cell Injury in Mouse Myoblast $C_2C_{12}$ Cells
Mouse 유래 $C_2C_{12}$ 세포주에서 청심연자탕(淸心蓮子湯)의 심근세포 손상 억제 효과
- Yoon, Hyeon-Deok (Department of Oriental Internal Medicine Daegu Haany University) ;
- Shin, Oh-Chul (Department of Oriental Internal Medicine Daegu Haany University) ;
- Shin, Yoo-Jeong (Department of Oriental Internal Medicine Daegu Haany University) ;
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Kim, Seung-Mo
(Department of Oriental Internal Medicine Daegu Haany University) ;
- Park, Chi-Sang (Department of Oriental Internal Medicine Daegu Haany University)
- 윤현덕 (대구한의대학교 심계내과학교실) ;
- 신오철 (대구한의대학교 심계내과학교실) ;
- 신유정 (대구한의대학교 심계내과학교실) ;
-
김승모
(간계내과학교실) ;
- 박치상 (대구한의대학교 심계내과학교실)
- Published : 2007.06.30
Abstract
Determination and differentiation of cells in the skeletal muscle lineage is positively regulated by cell-cell contact. differentiation proteins proposed to mediate this effect include both classical MyoD and MEF members : potential interactions between the promyogenic activities of these classes of protein, however, are unknown. We show here that MyoD and MEF, two promyogenic family members that determine to each other in a cis fashion, form ineraction with MyoD- and MEF. These proteins contain myosin heavy chains and are enriched at sites of cell-cell contact between myoblasts, Therefore, In differentiation of MyoD MEF from CST (Chungsimyeonjatang) interact dependently, suggesting that the interactions occur in a cis fashio : consistent with this conclusion, MyoD-mediated differentiation is required for myoblast to occur by CST. Inhibition in myoblasts of a MyoD by STP in its ability to associate with MEF interferes with differentiation as assessed by morphological and transcription level, suggesting that this interaction is functionally important in myogenesis. Also, some of the differentiation-mediated proteins that are required for myogenesis seem to be based on interdependent activities of promyogenic classical SMAD-subfamilly.