• Genomics & Informatics
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• v.2 no.2
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• pp.75-80
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• 2004

In a variety of animal species, the perinatal exposure of experimental animals to the 2,3,7,8-tetrachlorodibenzo­p-dioxin (TCDD) leads to the immune dysfunction, which is more severe and persistent than that caused by adult exposure. We report here the changes of gene expression and the identification of the marker-genes representing the dioxin exposure. The expressions of the transcripts were analyzed using the 11 K oligonucleotide­microarray from the bone marrow cells of male C57BL/6J mice after an intraperitoneal injection of $1{\mu}g$ TCDD/kg body weight at various time intervals: gestational 6.5 day(G6.5), 13.5 day(G13.5), 18.5 day(G18.5), and postnatal 3 (P3W)and 6 week (P6W). The type of self-organizing maps(SOM) representing the specific exposure dioxin could be identified as follows; G6.5D(C14), G13.5D(C0, C5, C10, C18), G18.5D(7): P3W(C2, C21), and P6W(C4, C15, C20). The candidate marker-genes were restricted to the transcripts, which could be consistently expressed greater than $\pm$2-fold in three experiments. The resulting candidates were 85 genes, the characteristics of that were involved in cell physiology and cell functions such as cell proliferation and immune function. We identified the biomarker-genes for dioxin exposure: smc -like 2 from SOM C14 for the dioxin exposure at G6.5D, focal adhesion kinase and 6 other genes from C0, and protein tyrosine phosphatase 4a2 and 3 other genes from C5 for G13.5D, platelet factor 4 from C7 for G18.5D, fos from C2 for P3W.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.749-754
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• 2000

PCR primers were designed based on consensus sequences of dTDP-D-glucose 4,6-dehydratase, one of the enzymes involved in the biosynthesis of deoxysugar. The PCR product (360 bp) was obtained from Thermus caldophilus GK24. Colony hybridization was carried out to the cosmid library constructed from T. caldophilus GK24 genomic DNA by the PCR product DNA fragment. We isolated a cosmid clone (pSMTC-1) that was subcloned to call pKCB series plasmid (BamHI fragments), partially sequenced and analyzed. pKCB80 (4.2 kb-BamHI DNA fragment) of them showed ORFs that was orfA, orfB, orfC and orfD. The orfABCD gene cluster is the deosysugar biosynthetic gene ; orfA (glucose-1-phosphate thymidylytransferase), orfB (dTDP-D-glucose 4,6-dehydratase), orfC (dTDP-4-keto-L-rhamnose reductase) and orfD (dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase). The gene cluster that was related in biosynthesis of dTDP-L-rhamnose was also identified by computer analysis, and we proposed that the biosynthetic pathway of deoxysugar analyzed from DNA sequencing of pKCB80 is from D-glucose-1-phosphate, dTDP-D-glucose, dTDP-4-keto-6-deoxy-D-glucose via dTDP-4-keto-L-rhamnose to dTDP-L-rhamnose.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.11
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• pp.1010-1018
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• 2013

The dual-stream based stereoscopic 3D broadcasting standard was developed in 2011. EBS broadcasted 3DTV experimental service according to this standard in April 2012. Dual-stream based 3DTV broadcasting is service-compatible and backward-compatible, so viewers who have legacy 2D HDTV can watch the broadcasting content as 2D. And 3DTV viewers can select watching 2D or 3D. Since 3D content production is not usual yet for terrestrial broadcasters, only some particular programs in specific periods will be broadcasted as 3D at the initiatory stage. For it, stable viewing condition is strongly required for both 2D viewers and 3D viewers. This paper suggests broadcasting transmission system structure and effective switching scheme for stable 2D and 3D viewing conditions in the case of dual-stream based terrestrial 3DTV broadcasting.

• BMB Reports
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• v.32 no.4
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• pp.358-362
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• 1999

dTDP-D-glucose 4,6-dehydratase as an oxidoreductase catalyzes the conversion of dTDP-D-glucose to dTDP-4-keto-6-deoxy-D-glucose, which is essential for the formation of 6-deoxysugars. dTDP-D-glucose 4,6-dehydratase shows remarkable sterochemical convergence in which displacement of the C-6 hydroxyl group by a C-4 hydrogen proceeds intramolecularly with inversion of configuration. The reaction mechanism is known to be oxidation, dehydration, and reduction by bases mediating proton transfer and $NAD^+$ cofactor. In this study, the bases in the active site domain are proposed to be His-79 and His-300 from a comparison of the peptides of the dehydratase and UDP-D-glucose epimerase. His-79 and His-300 were mutated to prepare the mutants H79L (mutation of histidine to leucine at the 79th amino acid) and H300A (mutation of histidine to alanine at the 300th amino acid) by site-directed mutagenesis. The H79L protein was inactive, showing that His-79 participates in the reaction mechanism.

• Korean Journal of Materials Research
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• v.11 no.2
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• pp.88-93
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• 2001

The thermal behavior and the crystallographic characteristics of an epitaxial $C49-TiSi_2$ island formed in a Si (001) substrate by $N_2$, treatment were investigated by X-ray diffraction (XRD) and high-resolution transmission electron microscopy (HRTEM). It was found from the analyzed results that the epitaxial $C49-TiSi_2$ was thermally stable even at high temperature of $1000^{\circ}C$ therefore did not transform into the C54-stable phase and did not deform morphologically. HRTEM results clearly showed that the epitaxial $TiSi_2$ phase and Si have the orientation relationship of (060)[001]$TiSi_2$//(002)[110]Si, and the lattice strain energy at the interface was mostly relaxed by the formation of misfit dislocations. Furthermore, the mechanism on the formation of the epitaxial $_C49-TiSi2$ in Si and stacking faults lying on the (020) plane of the C49 Phase were discussed through the analysis of the HRTEM image and the atomic modeling.

• Journal of the Korean Mathematical Society
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• v.45 no.4
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• pp.903-921
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• 2008

A continuous linear operator $T\;:\;X{\rightarrow}X$ is called hypercyclic if there exists an $x\;{\in}\;X$ such that the orbit ${T^nx}_{n{\geq}0}$ is dense. We consider the problem: given an operator $T\;:\;X{\rightarrow}X$, hypercyclic or not, is the restriction $T|y$ to some closed invariant subspace $y{\subset}X$ hypercyclic? In particular, it is well-known that any non-constant partial differential operator p(D) on $H({\mathbb{C}}^d)$ (entire functions) is hypercyclic. Now, if q(D) is another such operator, p(D) maps ker q(D) invariantly (by commutativity), and we obtain a necessary and sufficient condition on p and q in order that the restriction p(D) : ker q(D) $\rightarrow$ ker q(D) is hypercyclic. We also study hypercyclicity for other types of operators on subspaces of $H({\mathbb{C}}^d)$.

• Proceedings of the KIEE Conference
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• 2005.11b
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• pp.252-254
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• 2005

전력계통을 감시, 보호하기 위해서 해당 도메인에 맞게 전력감시 시스템을 운용한다. 전기품질을 위해서 전기품질감시 시스템을, 전력설비의 노후 정도, 교체기간을 감시하기 위해서 전력설비진단 시스템을 각각 운용한다. 최근 전력 IT 기술의 발달과 전력감시 시스템의 효율적 관리가 필요하게 되면서 국내외 선진사들은 전력시스템의 통합 솔루션을 소개, 개발하고 있는 추세이다. 본 논문에서는 전력감시 시스템들을 총합한 전력감시진단종합 시스템을 개 발하였고 이를 소개하고자 한다.

• Asian Journal of Turfgrass Science
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• v.10 no.3
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• pp.231-246
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• 1996

In order to elucidate the human impact on the distribution of cellular slime molds, samples were collected from 3 types of forest ; natural forests(Mt. Deogyu and Mt. Tsukuba), semi-natural forests(Seoul Great Zoo and Tama Zoo), artificial forests(Seoul National University and Tokyo Gakugei University) .The distribution of cellular slime molds in mountains was different from that of zoo and universities. In mountains, endemic species was occurred and species diversity was higher than in zoo and universities. In zoo and universities disturbed by human, Dictyostelium sphaerocephalum was occurred with higher importance value than in mountains. 6 species were selected to investigate the effect of temperature on froctification; Polysphondylium canlidum, D. delicatum. D. firmibasis, D. sphaerocephalum P. violaceum, D. purpureum. P. violaceum and D. purpureum had an optimum temperature for fructification around 25~3O˚C but the others around 22~23˚C. The degree of sensitivity to temperature was as follows; P. candidum >D. lelicatum > D. firmibasis > D. sphaerocephalum > P. violaceum > D. purpureum. Key words: Human impact, Cellular slime molds, Occurrence and distribution, fructification, Dictylostelium delicatum. Dictyostelium sphaerocephalum.

• Toxicological Research
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• v.33 no.4
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• pp.265-272
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• 2017

Aryl hydrocarbons such as 3-nitrobenzanthrone (NBA), 4-aminobiphenyl (ABP), acetylaminofluorene (AAF), benzo(a)pyrene (BaP), and 1-nitropyrene (NP) form bulky DNA adducts when absorbed by mammalian cells. These chemicals are metabolically activated to reactive forms in mammalian cells and preferentially get attached covalently to the $N^2$ or C8 positions of guanine or the $N^6$ position of adenine. The proportion of $N^2$ and C8 guanine adducts in DNA differs among chemicals. Although these adducts block DNA replication, cells have a mechanism allowing to continue replication by bypassing these adducts: translesion DNA synthesis (TLS). TLS is performed by translesion DNA polymerases-Pol ${\eta}$, ${\kappa}$, ${\iota}$, and ${\zeta}$ and Rev1-in an error-free or error-prone manner. Regarding the NBA adducts, namely, 2-(2'-deoxyguanosin-$N^2$-yl)-3-aminobenzanthrone (dG-$N^2$-ABA) and N-(2'-deoxyguanosin-8-yl)-3-aminobenzanthrone (dG-C8-ABA), dG-$N^2$-ABA is produced more often than dG-C8-ABA, whereas dG-C8-ABA blocks DNA replication more strongly than dG-$N^2$-ABA. dG-$N^2$-ABA allows for a less error-prone bypass than dG-C8-ABA does. Pol ${\eta}$ and ${\kappa}$ are stronger contributors to TLS over dG-C8-ABA, and Pol ${\kappa}$ bypasses dG-C8-ABA in an error-prone manner. TLS efficiency and error-proneness are affected by the sequences surrounding the adduct, as demonstrated in our previous study on an ABP adduct, N-(2'-deoxyguanosine-8-yl)-4-aminobiphenyl (dG-C8-ABP). Elucidation of the general mechanisms determining efficiency, error-proneness, and the polymerases involved in TLS over various adducts is the next step in the research on TLS. These TLS studies will clarify the mechanisms underlying aryl hydrocarbon mutagenesis and carcinogenesis in more detail.

• Food Science of Animal Resources
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• v.38 no.6
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• pp.1131-1143
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• 2018

This study aimed to investigate the drying yield, meat quality, oxidation stability and sensory properties of Hanwoo beef loin subjected to different dry-aging conditions. A total of 54 Hanwoo beef loins (bone-in, $6^{th}-13^{th}$ ribs) with fat cover at 2 d postmortem were assigned to four groups and hung in a dry-aging room at a controlled temperature ($2^{\circ}C-4^{\circ}C$), humidity (65%-85%) and air velocity (3 m/s). Four treatment conditions were tested; the respective temperature, humidity and treatment duration are as follows: T1, $2^{\circ}C$, 85%, 60 d; T2, $2^{\circ}C$, 65%, $20d+2^{\circ}C$, 75%, $20d+4^{\circ}C$, 85%, 20 d; T3, $2^{\circ}C$, 75%, $20d+4^{\circ}C$, 85%, 40 d; T4, $4^{\circ}C$, 85%, 60 d. The drying yield, total aerobic counts and fat contents increased, whereas the moisture content, meat color (CIE $L^*$, $a^*$, and $b^*$), and Warner-Bratzler shear force values decreased significantly during the aging period for all treatments (p<0.05). The cooking loss (%) did not change significantly until 40 d, and it was significantly higher in T1 and T4 than in T2 and T3 at 60 d (p<0.05). The water-holding capacity, pH and lipid oxidation [thiobarbituric acid reactive substance (TBARS) values] increased during aging (p<0.05). The sensory scores for tenderness, juiciness, flavor, and overall acceptability of dry-aged loin muscles increased as the aging period increased. T2 and T3 had significantly higher sensory scores and T1 had significantly lower scores for tenderness, juiciness, flavor, and overall acceptability at 20, 40, and 60 d for loin muscles (p<0.05).