• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5911-5913
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• 2013

Cyclamen coum is a traditional medicinal plant in the Turkey. Its anticancer properties and whether cyclamen extract induces any cytotoxicity in solid cancer cell lines have not been thoroughly investigated previously. Therefore we examined cytotoxic effects on cervical cells; HeLa and non small cell lung cancer cell, H1299, lines; Cyclamen extract induced cellular death of both HeLa and H1299 cells in a dose dependent manner. We also analyzed the capacity of cyclamen extract to induce apoptosis by the TUNEL method. Here, for the first time we report that the extract of Cyclamen coum, an endemic plant for Turkey, Bulgaria, Georgia and the Middle East can induce cytotoxicity via apoptosis in HeLa and H1299 cells. These results imply that cyclamen extract can be further analyzed to potentially find novel anticancer compounds.

• Research in Plant Disease
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• v.6 no.1
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• pp.33-35
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• 2000

A disease of Cyclamen persicum was found in Kangnung of Kang-won prefecture, Korea, 1999. Typical symptoms were dark green to brown lesions appeared on petals and leaves. Many spores appeared on the lesions under humid conditions. A Botrytis species was consistently isolated from the infected plant. Conidia were obvoid to ellipsoid, 1-celled, hyaline or pale brown. The conidia were about 0.9-16.0$\times$6.0-10.4㎛(mean, 1.27$\times$7.8㎛) under scanning electron microscope, many micro-projections appeared on the surface of conidia. The isolated fungus infected heathy Cyclamen persicu. The causal agent was identified as Botrytis cinerea. Gray mold of Cyclamen persicum was proposed to name this disease.

• Korean journal of applied entomology
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• v.32 no.4
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• pp.433-439
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• 1993

Morphological characteristics and taxohomy of the two most important Tarsonemid mutes in agriculture, cyclamen mite (Phytonemus pallidus (Banks), 1899) and Broad mite (Polyphagotarsonemus lalus Banks, 1904) are described and drawings of ventral and dorsal views of females and males of each species are provided. Cyclamen mite was collected from fatsia japonica, and Broad mite was collected from Capcicum annuum, Rhododennon schlippenbachil, impatrens sulianti, Ilex serrata, and Fraxinus rhynchophylla.

• Research in Plant Disease
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• v.9 no.2
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• pp.79-84
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• 2003

A wilt disease of commercial cyclamen (Cyclamen persicum) which grown in greenhouse was found in Gyeo-nggi province of Korea during the period from August, 2001 to July, 2002. The disease incidence was up to 42.7% in Kimpo, Gyeonggi province. The disease was more severe in ebb and flow irrigation system than con-ventional overhead flooding\'s. The wilted cyclamen plants showed the chlorosis of leaves and followed by the death. The vascular tissues of the infected basal stem and bulb were discolored with black streaks. The casual fungus was identified to be Fusarium oxysporum f. sp. cyclaminis on the basis of mycological characteristics. Effect of infected soil showed 100% infection rate when the cyclamen plants were grown in potting with infested soil. Examine resistant cultivar showed ‘Matis red’, ‘Schubert’, ‘Victoria’ and ‘Chopin’ were the resistant cultivars but most of cultivars were susceptible to Fusarium wilt. The control effect of chemicals for cyclamen wilt was not much efficient to curative effects even though. Benomyl WP and Fludioxonil SC on the Fusarium wilt had only preventive effects by soil drenching in the pot before inoculation of F. oxysporum. This is the first report on the fusarium wilt of cyclamen caused by F. oxysporum f. sp. cyclaminis in Korea.

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.213-213
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• 1995

• Plant Resources
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• v.7 no.3
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• pp.222-225
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• 2004

In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.479-484
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• 2000

This study was conducted to examine the effects of explant sources and plant growth regulators on mass propagation of Cyclamen persicum. Tuber, cotyledon, and petiole tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Shoots were not induced from calli on cotyledon and petiole explants cultured on MS medium containing various concentrations of 2,4-D or NAA. However, multiple shoots were formed directly from tuber explants cultured on the medium containing 0.5 and 1.0 mg/L 2,4-D or NAA. In MS medium with cytokinin alone, TDZ was more effective in shoot formation than BA or kinetin in all explants. The combinations of NAA and BA was found to be most effective in shoot formation from tuber, cotyledon and petiole explants. Especially, shoots were formed from all the tuber explants on the medium containing 0.5 mg/L of NAA and BA. Hormonal effects on root formation were examined by subculturing single shoots on MS medium containing NAA or IBA. The medium with 0.5 mg/L IBA was most effective in root induction and subsequent plantlet regeneration.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.195-199
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• 1997

Anthracnose symptoms were observed on the commercially cultivating ultivating cyclamen in Chonbuk province in 1995. The symptoms of infected flowers were small, circular and dark brown ring-spots or water-soaked lesions and gradually changed to lack blight. Mycelial colony of the isolates was light or whitish gray to dark gray on potato dextrose agar. Conidia were straight cylindrical and obtuse at the apex and measured 8.3~15.0${\times}$2.5~6.3 ${\mu}m$ in size. Appressoria were brown to dark brown and clavate, but most of them were irregular. Acervuli on lesions were brown, rounded and measured 50~140${\times}$32.5~90 ${\mu}m$ in size. The optimum temperatures for mycelial growth and conidial sporulation were ranged from 25 to 30$^{\circ}C$. Thus, based on mycological characteristics of the fungus, the causal agent of cyclamen anthracnose was identified as Colletotrichum gloeosporioides Penz.

• Journal of agriculture & life science
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• v.44 no.3
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• pp.7-14
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• 2010

Alternative subirrigation way, capillary wick system (CWS) was tested to reduce labor cost, waste water, contamination of ground water, and use of fungicide compared to overhead irrigation system (OIS). CWS helped reduce remarkably the working hours for watering from 4 hours in OSI to just 5 minutes. Labor cost was saved 98% in CWS compared to OIS. By the physical characteristics of various growing media, 1 coconut coir+2 perlite (v/v) mixture was selected because it had an ideal distribution of three phase, e.g. 1 solid: 1 liquid: 2 gas phase. Medium mixture containing scoria had so high bulk and particle density to hurt root. In bark-containing medium, the liquid phase and the percent saturation of liquid phase with time elapsed was lower than that of other mixture. It meant that the mixture contained very low level of water. Application of CWS for cyclamen pot production played an important role in reducing the incident of fusarium wilt symptom from 18% in conventional over watering system to 4%. Cyclamen pot irrigated by capillary wick had shorter petiole and more leaves than those by overhead watering. As a result, this system was highly beneficial to get uniform pot products with high quality. It improved water and nutrient solution efficiency relative to conventional overhead irrigation system (OIS).

• Research in Plant Disease
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• v.20 no.3
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• pp.223-226
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• 2014

Sclerotium rot caused by Sclerotium rolfsii occurred on Cyclamen europaeum grown at the experimental greenhouse of Gyeongsangnam-do Agricultural Research and Extension Services in April 2013. Infected plants showed water-soaked appearance, wilting, and rotting; severely infected plants eventually died. White mycelial mats spread over lesions, and sclerotia were formed on leaves, petioles, and flower stalks near soil line. On the basis of mycological characteristics, ITS rDNA sequence analysis, and pathogenicity to host plants, this fungus was identified as S. rolfsii Saccardo. This is the first report of sclerotium rot on C. europaeum caused by S. rolfsii in Korea.