• Journal of Applied Biological Chemistry
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• 제66권
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• pp.338-344
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• 2023

Curcuma longa is a plant belonging to the genus Curcuma and is distributed across various Asian regions. This plant is widely known for its rhizomes, which possess a variety of pharmacological properties. However, although the leaves and stems of this plant also contain several health-promoting secondary metabolites, very few studies have characterized these compounds. Therefore, our study sought to quantify the secondary metabolites from the leaves and stems of Curcuma longa L. (LSCL) using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and high-performance liquid chromatography (HPLC). Our LC-ESI-MS analyses detected twenty-one phenolic compounds in the LSCL, among which fifteen compounds were detected via HPLC analysis. Four compounds, namely vanillic acid (0.129 mg/g), p-coumaric acid (0.431 mg/g), 4-methylcatechol (0.199 mg/g), and afzelin (0.074 mg/g) were then quantified. These findings suggest that LSCL is rich in secondary metabolites and holds potential as a valuable resource for the development of functional and nutritional supplements in the future.

• 한국약용작물학회지
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• 제22권5호
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• pp.378-383
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• 2014

Curcuma longa L. leaf was extracted by water at $60^{\circ}C$ for 12 hours after being treatment of Ultra High Pressure under 500 MPa for 5-15 minute. The high pressure extraction for 15 minute (HPE15) was measured the highest extraction yield of 25.48% (w/w), compared to those from conventional extraction methods. The HPE15 showed the lowest cytotoxicity as 11.97% in adding $1.0mg/m{\ell}$ of concentration. Also, HPE15 was measured the highest inhibition of hyaluronidase as 44.48% in adding $1.0mg/m{\ell}$. In addition, The production of NO from macrophages was measured as $7.06{\mu}M$ in adding $1.0mg/m{\ell}$ of HPE15, which was lower than the those from others processes. Finally, HPE15 significantly reduced up to $649.44pg/m{\ell}$ of $ProstaglandinE_2$ production from UV-irradiation. These results suggest that the Curcuma longa Linne leaf extract from high pressure process might enhance the skin immune activities possibly by high elution of active components than other processes.

• 한국작물학회지
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• 제48권6호
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• pp.438-441
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• 2003

울금의 기내대량번식을 위한 경정배양에 알맞은 기본배지의 종류와 기내증식 및 생장에 미치는 식물생장조절제의 농도를 구명하고자 본 실험을 수행한 결과는 다음과 같다. 울금의 경정은 MS(Murashige and Skoog)기본배지에 치상한 경우 Wanspojen, LS, White 배지보다 shoot와 root의 형성율이 높은 경향이었다. 기내의 유식물체 생장은 오옥신(NAA 0.5-l.0 $\textrm{mg}/\textrm{l}$)과 사이토키닌(BA 1.0-5.0 $\textrm{mg}/\textrm{l}$) 혼용처리가 효과적이었다.

• 한국식품영양과학회지
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• 제44권8호
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• pp.1114-1120
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• 2015

본 연구에서는 울금의 메탄올 추출물을 제조하고 3T3-L1 지방세포 모델을 이용하여 세포 수준에서 울금 인조쌀의 지방구 형성 조절을 통한 지질축적 억제능을 평가하였다. 더 나아가 C57BL/6J 마우스에 고지방식이와 함께 인조쌀, 울금 추출물이 첨가된 인조쌀을 공급하였을 때 체중과 지질대사의 변화를 관찰하였다. 세포 실험의 결과로부터 울금 메탄올 추출물을 처리한 후 $20{\mu}g/mL$의 농도에서 유의적인 지방구형성 억제를 관찰하였다. 동물 실험에서는 식이효율을 측정한 결과 인조쌀을 섭취한 군에서 그 효율이 낮아졌으며, 특히 울금 인조쌀을 섭취한 군에서 유의적으로 감소하였다. 부고환 지방 무게는 인조쌀 섭취군에서는 고지방섭취군과 차이를 보이지 않았지만, 울금 인조쌀을 섭취한 군에서는 유의적으로 낮아졌다. 혈청 지질 수준은 인조쌀군과 울금 인조쌀군 모두 고지방식이군에 비하여 개선되는 결과를 보였다. 혈청 중성지방은 인조쌀군과 울금 인조쌀군 모두유의적으로 낮아졌으며, 혈청 콜레스테롤 함량은 고지방식이군에 비해 인조쌀군에서 감소하는 경향을 보였고 울금 인조쌀군에서는 유의적으로 감소되었다. 이러한 결과를 통해 울금 이 첨가된 인조쌀에 다량 함유된 식이섬유소와 여러 생리활성물질이 지질대사를 개선시키고 지방 축적을 억제하여 일상식을 통한 체내 지질 수준 개선 효과를 기대해 볼 수 있을 것이라 사료된다.

• 한국약용작물학회지
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• 제28권1호
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• pp.47-55
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• 2020

Background: Curcuma longa L., a perennial crop originating from tropical and subtropical region, including India, is noted for its important medicinal properties. However, C. longa plants are unable to endure the winter season in Korea, and its rhizomes were invariably succumb to fungal infection when stored in polyvinyl bags. In this study, we accordingly sought to develop a C. longa variety capable of producing high rhizome yields and to identify stable conditions under which rhizomes can be stored in Korea. Methods and Results: We evaluated the agronomic characteristics of nine C. longa germplasms and examined the effects of storing rhizomes at different temperatures (4℃ to 24℃) in paper bags or plastic baskets. We found that the finger rhizomes was higher in CUR02, CUR03, and CUR06 germplasms than those of other groups. Furthermore, in terms of yield per 1 ㎡, the weights of the finger rhizomes and tuberous roots were significantly higher in CUR09 (3.4 ㎏/㎡) and CUR04 (678.7 g/㎡) than those of other groups. Therefore, we consider that these C. longa germplasms might be useful as breeding material. Although the fresh weights of the rhizomes were slightly reduced when stored in paper bags and a plastic baskets at 10℃ to 15℃, there was no evidence of fungal decomposition or sprouting, which is observed when using a conventional storage method. Conclusions: The results of this study indicate that the selected C. longa germplasms can provide a useful source of breeding material for the development of high yielding varieties and that a temperature ranging from 10℃ to 15℃ and the use of paper bags or plastic baskets provide stable post-harvest storage conditions for C. longa rhizomes.

• 대한의생명과학회지
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• 제25권3호
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• pp.247-255
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• 2019

Curcuma longa L. (C.L), Curcuma aromatica Salisb. (C.A) and Zingiber officinale Rosc. (Z.O) of Zingiberaceae plants which are well known as effects of natural anti-oxidant, anti-cancer and anti-inflammatory. We examined that the Zingiberaceae plants are involved in development and modulation of orofacial pain in rats. Male, 7- to 8-week-old, Sprague-Dawley rats weighing 240~280 g were used in this study. Experiments were performed using acute pain model that was caused by the injection of 5% formalin into the right vibrissa pad. The number of scratching or rubbing to the injection site was recorded for 9 consecutive 5-minute intervals following injection of formalin. The experimental groups were acute orofacial inflammatory pain; control group (formalin, 5%), vehicle group (5% formalin after sodium carboxymethyl cellulose), single administration group, single mixed administration group, repeated administration group. The experiments were performed various concentrations of Zingiberaceae plants extract. Therefore, oral administration of C.L, C.A, and Z.O (p.o., concentrations of 12.5, 25 mg/mL) in orofacial inflammatory pain model substantially decrease the nociceptive behavior in a concentration dependent manner. And it tended to decrease at low concentration (12.5 mg/mL) of single mixed and repeated administration more than single administration. This result means that Zingiberaceae plants extract affects the modulation of acute orofacial inflammatory pain. Thus, Zingiberaceae plants extract may be a potential therapeutic treatment for orofacial inflammatory pain.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1500-1504
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• 2009

Effects of methanol extract from turmeric (Curcuma longa L.) (CME) on underlying mechanisms of lipolysis were investigated in 3T3-L1 adipocytes. Compared to the control, lipid accumulation with 72 hr treatment of CME at the concentration $20\;{\mu}g/mL$ was significantly decreased by 19.9% as quantified by Oil red O dye. Intracellular triglyceride (TG) content was also lowered by 19.3%. To determine the mechanism for TG content reduction, glycerol release level was measured. Incubation of 3T3-L1 adipocytes with 15 and $20\;{\mu}g/mL$ of CME significantly elevated the level of free glycerol released into the cultured medium by 20.4 and 28.6%, respectively. In subsequent measurements using quantitative real-time polymerase chain reaction (PCR), mRNA levels of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) were significantly increased by 21.2 and 24.9%, respectively, at the concentration $20\;{\mu}g/mL$. Results indicated that CME stimulated lipolysis through induction of HSL and ATGL mRNA expressions, resulting in increased glycerol release.

• 생약학회지
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• 제44권3호
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• pp.253-256
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• 2013

Six flavonoid compounds were isolated from the underground parts of Curcuma longa (Zingiberaceae) through repeated column chromatography. Their chemical structures were elucidated as kaempferol-3-O-${\alpha}$-L-rhamnopyranosyl(1${\rightarrow}$2)-O-${\alpha}$-L-rhamnopyranoside (1), quercetin-3-O-${\alpha}$-L-rhamnopyranosyl(1${\rightarrow}$2)-O-${\alpha}$-L-rhamnopyranoside (2), quercetin-3-O-${\beta}$-Dglucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside (3), kaempferol-3-O-${\alpha}$-L-rhamnopyranoside (4), quercetin-3-O-${\alpha}$-L-rhamnopyranoside (5), and quercetin (6), respectively, by spectroscopic analysis. These compounds were isolated for the first time from this plant.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.484-485
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• 2006

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.486-487
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• 2006