• 제목/요약/키워드: Culture-negative

검색결과 1,937건 처리시간 0.028초

비임균성 뇨도염 환자에서 Chlamydia trachomatis 검출방법에 관한 연구 (배양법, 효소면역법 및 직접면역형광법의 비교 관찰) (A Comparision of the Sensitivities of Culture, Chlamydiazyme and Direct Immunofluorescence Staining for Chlamydia trachomatis in Non-gonococcal Urethritis)

  • 최태열;김춘원;김중환
    • 대한미생물학회지
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    • 제21권3호
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    • pp.393-397
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    • 1986
  • Chlamydia trachomatis is one of the most common cause in non-gonococcal urethritis. There are several diagnostic methods for Chlamydia trachomatis; culture method using McCoy cell, enzyme immunoassay and direct immunofluorescence staining etc. We have studied a sensitivities of culture, chlamydiazyme and direct immunofluorescence staining(DIF). 85 male patients previously conformed to non-gonococcal urethritis have been selected in this study. Three samples were concurrently collected in the same patient. First sample was used to inoculation in McCoy cell, 2'nd sample was used to Chlamydiazyme test and 3'rd sample was used to direct immunofluorescence staining method. The results are following. 1. All culture, Chlamydiazyme and DIF positive cases are 15/85(17.7%). 2. Culture and Chlamydiazyme positive but DIF negative cases absent. 3. Culture and DIF positive, but Chlamydiazyme negative cases are 2/85(2.4%). 4. Chlamydiazyme and DIF positive, but culture negative cases are 9/85(10.6%). 5. Culture positive, but Chlamydiazyme and DIF negative cases are 6/85(7.1%). 6. Chlamydiazyme positive, but culture and DIF negative cases are 7/85(8.2%). 7. DIF positive, but culture and Chlamydiazyme negative cases are 3/85(3.5%). 8. All culture, Chlamydiazyme and DIF negative cases are 43/85(50.1%). In summarized, anyone positive cases of culture, Chlamydiazymc and DIF are 42/85(49.9%).

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족관절 골절 치료에 사용한 금속 내고정물 제거 수술 시 시행한 균 배양 검사의 결과 (Results of Culture Test at the Time of Removal of Metal Implants Used for Ankle Fracture Management)

  • 정형진;배서영;유재하
    • 대한족부족관절학회지
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    • 제18권2호
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    • pp.68-71
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    • 2014
  • Purpose: The purpose of this study is to report the results of culture test at the time of removal of metal devices used for management of ankle fractures and for analysis of contributing factors. Materials and Methods: We reviewed medical records of 132 patients with lower tibia and ankle fracture who had their metal devices removed during the period from January 2010 to February 2014. Patients with clinical signs of infection were excluded. Culture test was performed by taking the granulation tissue around the metal device at the time of removal. We divided the subjects into two groups, culture positive and negative. We then performed a retrospective review of each medical record of multiple factors that might contribute to the culture results, including laboratory results, medical history, material and size of metal device, indwelling period, and whether or not it was open injury. Results: Among 132 cases, six were culture positive. Enterococcus was detected in two cases and the others were Staphylococcus. No significant difference in medical history of patients and laboratory results, including C-reactive protein level, was observed between the culture positive and negative group. Culture positive rate was 5.4% in titanium and 3.9% in stainless steel. In terms of metal size, culture positive rate was 5.1% in small plates, 6.7% in large plates, and culture negative in intramedullary nails. The average indwelling period of metal device was 61.5 weeks in the culture positive group, and 68.6 weeks in the negative group. Nine cases were open fractures and all were in the culture negative group. Conclusion: Whether or not the culture result was positive, there were no meaningful contributing factors. Presence of bacterium on the metal device could not be screened by any laboratory results or other factors.

The Effect of Job Stress and Job Exhaustion on Job Performance of Body Guards and Security Guards due to Organizational Culture

  • Kim, Sang-Jin
    • 한국컴퓨터정보학회논문지
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    • 제25권7호
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    • pp.193-202
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    • 2020
  • 본 연구는 경호경비원의 조직문화에 따른 직무스트레스와 직무소진이 직무성과에 미치는 영향을 규명하기 위하여 경호경비원 320명을 대상으로 설문조사를 실시하였다. 이 연구를 통하여 얻은 결론은 다음과 같다. 첫째, 조직문화에서 혁신문화는 직무스트레스에 통계적으로 .01수준에서 부적(-)인 영향을 미친다. 둘째, 조직문화는 직무성과에 직접적인 영향을 미치지 않는다. 셋째, 조직문화 가운데 시장문화는 직무성과에 통계적으로 .01수준에서 부적(-)으로 영향을 미친다. 넷째, 직무스트레스는 직무성과에 통계적으로 .001수준에서 부적(-)으로 영향을 미친다. 다섯째, 직무소진은 직무성과에 통계적으로 .001수준에서 부적(-)으로 영향을 미친다. 여섯째, 직무스트레스는 조직문화의 혁신문화와 직무성과의 관계에서 통계적으로 .01수준에서 부적(-) 매개효과가 있다. 일곱째, 직무소진은 조직문화와 직무성과의 관계에서 통계적으로 유의한 영향이 없다.

흡인이 의심되는 급성 중독환자에서 병원균 양성 배양 결과의 예측 (Prediction of pathogen positive-culture results in acute poisoning patients with suspected aspiration)

  • 백승하;박성욱
    • 대한임상독성학회지
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    • 제20권2호
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    • pp.75-81
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    • 2022
  • Purpose: This study sought to compare the characteristics of patients with pathogen-positive and negative cultures, and to investigate factors predicting pathogen-positive culture results in patients of acute poisoning with suspected aspiration. Methods: Consecutive patients with acute poisoning admitted to an intensive care unit between January 2016 and December 2018 were retrospectively studied. Respiratory specimens were collected from the enrolled patients at the time of the suspected aspiration. We compared the characteristics of patients with pathogen-positive and negative culture results and analyzed the causative pathogens. Results: Among the 526 patients, 325 showed no clinical features that could be attributed to aspiration, and 201 patients had clinical features suggestive of aspiration. Of these, 113 patients had pathogen-positive culture, 61 were negative, and the specimens of 27 patients contained poor-quality sputum. In univariate analysis, patients with a positive culture showed a longer time to culture from ingestion (p=0.01), faster heart rate (p=0.01), and higher partial pressure of arterial oxygen to the fraction of inspired oxygen (PaO2/FiO2) (p=0.02) than patients with negative culture. Multivariate analysis demonstrated that PaO2/FiO2 (adjusted odd ratio, 1.005; 95% confidence interval [CI], 1.002-1.008; p=0.005) was a significant risk factor for pathogen-positive culture. The area under the receiver operating characteristic curve of PaO2/FiO2 was 0.591 (95% CI, 0.510-0.669, p=0.05). Gram-negative pathogens (GNPs) were predominant and at least one GNP was observed in 84 (73.3%) patients among those with pathogen positive culture. Conclusion: We failed to find any clinical factors associated with positive culture results. Antibiotics that cover GNPs could be considered when deciding the initial antibiotic regimen at the time of suspected aspiration.

문화상호주의적 관점에서 본 베트남 다문화가족의 가족생활적응 사례연구 (A Case Study on Adaptability Factors in Family Life for Vietname Multi-cultural Families in Korea in Terms of the Inter-culturalism)

  • 신유경;장진경
    • 가족자원경영과 정책
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    • 제14권3호
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    • pp.109-122
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    • 2010
  • The present study aims to investigate cultural similarities and differences that influence interracial couples (between Korean men and Vietnamese women) when adapting to Korean family culture in terms of interculturalism. The interviewees consisted of three generations. This study used in-depth interviews. Data was collected from January to August 2008. 15 families (45 people) were used for analysis. Results were as follows: (1) the patriarchal culture of the two nations had a positive influence on the adaptation of members during family interactions(2) the culture of filial piety is a value system that both nations believe is important. However, different rituals had a negative influence on the practice of filial piety (3) the community-oriented culture is regarded as important in the two nations. However, Vietnamese wives cannot lead a free community life because Koreans see them in negative terms (4) the Vietnamese traditional wedding custom called "NopJjeOh" had a negative influence on the adaptation to the Korean family culture (5) the preference for sons in Vietnam is not as high as in Korea. This difference had a negative influence on adaptation (6) clear differences between the daily lives of the two nations had a negative influence on adaptation to Korean family culture. In conclusion, Korea and Vietnam have many similarities in culture, which enable Vietnamese wives to adapt to Korean culture more easily than other foreign wives. If various programs are developed to promote the mutual understanding between both cultures based on these research findings, it would further contribute toward social integration in Korea.

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대학병원 종사자의 조직구조, 조직문화에 따른 조직갈등의 관계에 대한연구 (The Relation of Organization Structure, Organization Culture and Organization Conflict of University Hospital Staff Members)

  • 이창호;조덕영;김윤진
    • 보건의료산업학회지
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    • 제9권1호
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    • pp.95-107
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    • 2015
  • The purpose of this study is to analyze the characteristics of hospital organization structure, organization culture, organization conflict and the interrelation of influential factor in organization conflict. The data for this study were collected through a self-administered survey with a structured delivered to 755 subjects including several nursing staff members, administration staff members and medical technicians at six hospitals. The structural equation model was used to examine the relation of organization structure, organization culture and organization conflict. The results of the analysis in this study were as follows. First, organization structure had a positive influence on organization conflict, especially in terms of complexity and centralization. But, formalization had a negative influence on organization conflict. Second, organization culture had a positive influence on organization conflict, especially in terms of group culture, developmental culture, and hierarchical culture. But, group culture had a negative influence on organization conflict. Third, organization structure had positive influences, especially on organization culture complexity, centralization, and formalization. But, centralization had negative influences too.

Exploring Impact of Positive/Negative Valence Order on Repeated Exposure to Suspenseful Stories

  • Chang Ui Chun
    • International Journal of Advanced Culture Technology
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    • 제11권3호
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    • pp.182-189
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    • 2023
  • This study investigates the psychological effects of positive/negative valence order in repeated exposure to a suspenseful text. Specifically, the study seeks to understand how the order in which positive and negative elements are presented in a narrative impacts the experience of suspense, arousal, and enjoyment in readers or listeners. Using a suspenseful short story, participants were exposed to narratives with varying valence orders in a repeated-measures design. The study employed self-report questionnaires and psychophysiological measurements to capture participants' psychological responses. The results supported the hypothesis that repeated exposure impacts suspense, with negative valence enhancing suspense and arousal. Moreover, the order of valence influenced participants' psychological responses, indicating that positive valence can mitigate the impact of repeated exposure. However, the influence on enjoyment was not significant. Psychophysiological measures, specifically skin conductance level (SCL), revealed trends of habituation over repeated exposure. The findings underscore the significance of negative valence in heightening suspense and suggest directions for future research in exploring diverse factors that contribute to suspense in both fictional and real-life contexts.

Evaluation of Negative Results of BacT/Alert 3D Automated Blood Culture System

  • Kocoglu M. Esra;Bayram Aysen;Balcl Iclal
    • Journal of Microbiology
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    • 제43권3호
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    • pp.257-259
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    • 2005
  • Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (BPA) and anaerobic (BPN) media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in $5\%$ sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar. Organisms growing on these subcultures were subsequently identified using both Vitek32 (bioMerieux, Durham, NC) and conventional methods. Twenty four $(2.6\%)$ of the 904 subcultures grew on the subculture media. The majority $(83.3\%)$ of these were determined to be gram-positive microorganisms. Fourteen $(58.3\%)$ were coagulase-negative staphylococci, two $(8.3\%)$ were Bacillus spp., one $(4.2\%)$ was Staphylococcus aureus, and one $(4.2\%)$ was identified as Enterococcus faecium. Streptococcus pneumoniae and Neisseria spp. were isolated together in two $(8.3\%)$ vials. Gram-negative microorganisms comprised $12.5\%$ of the subcultures, of which two $(8.3\%)$ were found to be Pseudomonas aeruginosa, and one $(4.2\%)$ was Pseudomonas fluorescens. The other isolate $(4.2\%)$ was identified as Candida albicans. We conclude that the subculture of negative results is valuable in the BacT/Alert 3D system, especially in situations in which only one set of blood cultures is taken.

수술실 간호사가 지각하는 간호조직문화 유형, 셀프리더십 및 소진 간의 관계 (Relationship among Types of Nursing Organizational Culture, Self-leadership and Burnout as Perceived by Perioperative Nurses)

  • 임민경;성영희;정정희
    • 간호행정학회지
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    • 제23권2호
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    • pp.170-180
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    • 2017
  • Purpose: This study was designed to assess the types of nursing organizational culture, and degree of self-leadership and burnout as perceived by perioperative nurses, and to identify correlations between these variables. Methods: Participants were 155 nurses from 3 tertiary hospitals in Seoul and data were analyzed using descriptive statistics, ANOVA, Scheffe, and Pearson correlation coefficient. Results: In the types of nursing organizational culture, the mean score for hierarchy-oriented culture was 3.57, and for self-leadership, 3.61 and for burnout, 2.87. In the relationship between the types of nursing organizational culture, self-leadership and burnout, relation-oriented culture (r=.24, p<.01), innovation-oriented culture (r=.23, p<.01) and task-oriented culture (r=.22, p<.01) had a slight positive correlation with self-leadership in that order. The innovation-oriented culture (r=-.29, p<.01) and relation-oriented culture (r=-.42, p<.01) among the types of nursing organizational culture showed a negative correlation with burnout while the hierarchy-oriented culture (r=.28, p<.01) showed a positive correlation with burnout. Self-leadership showed a negative correlation with burnout (r=-.42, p<.01). Conclusion: The results show that nurses in operating rooms have a high awareness of hierarchy-oriented culture that affects burnout in a negative way while having a low awareness of relation-oriented culture that has a positive influence on burnout.

근관내(根管內) 기계조작(器械操作)이 감염확산(感染擴散)에 미치는 영향(影響) (THE INFLUENCE OF INSTRUMENTATION IN THE CANAL ON THE EXTENSION OF INFECTION)

  • 김영해
    • Restorative Dentistry and Endodontics
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    • 제10권1호
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    • pp.177-181
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    • 1984
  • This study was to confirm the influence of intracanal instrumentation whether the pulp infection could be extended to periapical tissue. Fifty three teeth (24 badly decayed and infected, 29 sound teeth) were employed for this experiment and grouped as follows; 1. The specimen taken from the tip of 12 infected pulp in which reamer was inserted to the canal up to apical 1/3 and cultured as long as 48 hours. After 24 hours culture 11 cases were positive and 1 ease was negative but the time of incubation elapsed as 48 hours a negative case turned to positive. 2. Broth immersed paper disc was placed for 1 minute on the tip of 12 infected teeth with a reamer inserted to the apical end and cultured as usual manner in the incubator. At 24 hour culture the growth was significant in 9 cases and after 48 hours total 12 cases were positive. 3. Reamer was inserted to apical 1/3 on 14 sterile pulp canals and specimens obtained from the root tip were cultured for 24 and 48 hours. The results on both group were negative. 4. Similar maner with No.3 except reamer tip was rest exactly at the apex revealed only 2 cases of positive at 48 hour culture. 5. The tip of 24 reamers which reached to apical 1/3 and apex of infected canal were cultured for 24 and 48 hours. At 24 hour culture the growth was evident. 6. The tip of 14 reamers which inserted to apical 1/3 of sterile canal showed negative at 24 hour culture. The 15 cases of the tip which reached to the apex of sterile canal were found negative except 3 positive cases at 48 hour culture.

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