• 한국식생활문화학회지
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• 제15권2호
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• pp.123-137
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• 2000

Korea market of food service industry has been increased greatly since early 90's with the emergence of professional catering business and the expansion of school food service. Because of the characteristics of food service establishment, feeding many people at a time, there is always a high potential of foodborne outbreak which requires special sanitary management to prevent. Currently, many catering businesses are interested in HACCP(Hazard Analysis Critical Control Points), a new sanitary management system, and are trying to develop HACCP Plans for their food service establishment. Microbiological hazard analysis, a most important step for developing HACCP Plan, provides also basic information for evaluating sanitary aspect of prepared foods and their materials. There are not extensive data of microbiological hazard analysis for food service operations, especially for prepared foods and raw materials, which are helpful for developing a HACCP Plan. The extensive microbiological hazard analyses for 65 prepared foods and 51 raw materials selected from a couple of food service establishment were performed in this research. These results will provide useful information to determine hazards for developing a HACCP plan, and also to understand sanitary status for prepared foods and raw materials in food service establishment.

• 한국식생활문화학회지
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• 제6권2호
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• pp.137-140
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• 1991

PALFAZE is one of ZE. ZE is One of the Chinese Sause. Especially PALFAZE is made from eight materials and used for the slices raw fish or raw meat. At first ZE was made from pickled vegitables that minced, or added vineger, some spices. But later when the garic-growing was spreaded it was changed to be made from minced garic, ginger mixed in vineger or added some spices, starch. Especially PALFAZE is made from garic, ginger, salt, vineger, mamanufactured plum, dried orange rind, boild chestnut, boiled rice.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권4호
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• pp.264-268
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• 2001

Aggregates of specific cells are often regarded as better from in artificial organs and mammalian cell bioreactors in terms of cell-specific functionality. In this study, the morphology and liver specific functions of freshly harvested primary rat hepatocytes, which were cultivated as spheroids and entrapped in a synthetic thermo-reversible extracellular matrix, were examined and compared to a control (hepatocytes in single cell form). A copolymer of N-isopropylacrylamide(98 mole % in feed) and acrylic acid (poly (NiPAAm-co-AAC)), a thermo- reversible copolymer gel ma- trix, was used to entrap hepatocytes either in spheroids or single cells. During a 7-day culture pe-riod, the spheroids maintained higher viability and produced albumin and urea at a relatively con-stant rate, while, the single cell culture showed a slight increase in cell numbers and a reduction in albumin secretion Hepatocytes cultrured as spheroids present a potentially useful three-dimensional cell culture system for application in bioartificial liver device.

• 한국약용작물학회지
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• 제12권5호
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• pp.366-371
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• 2004

Aconitine alkaloids produced from cell suspension cultures of Aconitum napellus for the first time. The effects of various culture conditions on cell biomass and aconitine accumulation in cell suspension cultures were investigated. Suspension cell cultures of A. napellus were established by transferring callus tissues from leaf explants onto liquid MS medium supplemented with $1\;mg/l$ NAA and $0.1\;mg/l$ kinetin. Among the culture media tested, MS medium had a pronounced effect on cell growth and aconitine accumulation. The maximum dry cell weight was obtained at inoculum size of 3 g (FCW) per flask and in MS medium supplemented with 5% sucrose after 8 weeks. The addition of salicylic acid (SA) and yeast extract (YE) in the MS medium enhanced aconitine accumulation. Using a proper combination of culture condition and supplements, aconitine content could reach 0.043% (dry weight basis), that was $2.5{\sim}3$ fold higher that detected in control cultures.

• The Plant Pathology Journal
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• 제24권3호
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• pp.328-336
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• 2008

Out of various fungi and bacteria tested for inhibition of Tobacco mosaic virus(TMV) infection using Nicotiana tabacum cv. Xanthi-nc, a bacterial isolate JB-l, identified as Pseudomonas putida had a strong direct inhibitory activity against the TMV infection. Its systemic or indirect activity was also noted at more than a half level of the direct control efficacy. Disease severity was reduced significantly in the susceptible tobacco N. tabacum cv. NC 82 by the treatment of the bacterial culture filtrate, somewhat more by the pretreatment than by simultaneous treatment, probably by inhibiting the TMV transmission and translocation in the plants, showing negative serological, which responses in the viral detection by DAS-ELISA. TMV-inhibitory substances from P. putida JB-1 were water-soluble, stable to high temperature(even boiling), and to a wide range of pH. As proteinase K nullified their antiviral activity, the TMV inhibition activity of P. putida may be derived from proteinaceous materials. In electron microscopy, TMV particles treated with the JB-1 culture were shown to be shrunken with granule-like particles attached on them. All of these aspects suggest that P. putida JB-1 may be developed as a potential agent for the control of TMV.

• BMB Reports
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• 제34권5호
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• pp.421-427
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• 2001

Osteoclasts, cells primarily involved in bone resorption, originate from the hematopoietic precursor cells of the monocyte/macrophage lineage and differentiate into multinucleated mature forms. We developed an in vitro osteoclast culture system using embryonic chicken bone marrow cells. This culture system can be utilized in studies on the differentiation and function of osteoclasts. Phosphatidylinositol 3-kinase (PI3-kinase) and mitogen-activated protein kinases (MAPKs) have been implicated in diverse cellular functions including proliferation, migration, and survival. Using the developed avian osteoclast culture system, we examined the involvement of these kinases in osteoclast differentiation by employing specific inhibitors of the kinases. We Found that the inhibition of the PI 3-kinase, p38, or ERK interfered with osteoclast formation, suggesting that the signaling pathways that involve these molecules participate in the process of chicken osteoclast differentiation.

• 한국콘텐츠학회논문지
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• 제20권12호
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• pp.681-692
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• 2020

본 연구는 의사소통 교수법의 흐름 안에서 출판된 <세종한국문화>라는 한국어 문화교재를 분석하여 문화교육과 표현활동의 통합적 다양화 방안을 제안하였다. 본 연구는 문화인식, 문화 문식력을 넘어서 문화 능력의 신장이라는 최종 목표를 설정하고 해당 교재의 주제 분포, 말하기 쓰기 등의 표현활동 유형을 분석하였다. 학습자가 속해 있는 두 문화의 단순한 비교를 넘어서 창조적 문화 생산 활동을 지향하는 문화능력의 신장은 표현활동과의 통합이 그 목표를 이루는 데 매우 효과적인 방안임을 전제로 하였다. 이 교재는 시각자료를 풍성하게 제시하면서 성취문화보다는 행동문화와 정보문화의 제시를 중점으로 삼고 있으며 초급 단계보다 높은 단계에서도 활용 가능하도록 다양한 수준의 매체 읽기 자료를 배열한 점을 그 특징으로 지적하였다. 또한 교재 속 표현활동 유형을 분석하여 말하기와 쓰기 영역의 질문형식이 학습자들의 표현활동을 몇 가지 유형에 제한시키고 있음을 발견하였다. 문화 이해와 문화 문식력 단계에서 문화 능력의 단계로 나아가기 위해서 질문 형식의 다양화, 표현 활동의 확장, 통합 활동의 창조적 적용 등을 제안하였다.

• 한국문화재보존과학회:학술대회논문집
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• 한국문화재보존과학회 2007년도 제26회 추계학술대회
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• pp.51-52
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• 2007

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.237-242
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• 2007

Barley anther culture is hard working to plating picking out anther from the glume and demand long time comparing to be short available development stage for effective culture. Also, it has been treatment massive materials due to low plantlet comparing to get desirable plants intensively. Consequently, this experiment was carried out trying to be more high barley anther culture effectively in terms of save plating effort. Plating materials and culture temperature affected anther culture efficiency are among the inoculation tissues or organs such as anthers, spikelets and whole panicles, culture efficiency was higher with spikelets in two-rowed than six-rowed barley due primarily to a lower contamination, and calli were induced within 30 to 50 days. Callus induction and plant regeneration rates were higher in cultures at $25^{\circ}C$ than at $15^{\circ}C$ and $20^{\circ}C$. Days to callus induction were 25 to 50 days at $25^{\circ}C$ and 50 to 60 days at $20^{\circ}C$.

• 한국항공운항학회지
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• 제23권1호
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• pp.1-7
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• 2015

The aircraft maintenance demands a lot of aviation mechanics in urgent according to the high season and low season in seasonal demand adjusted, unplanned maintenance work such as AD (airworthiness directives) and troubleshooting of aircraft system fault. The advent of Super-Large Aircraft such as A380 with aircraft materials, power plant and avionics systems which were not in previous models must be expected to increase the complexity of the aircraft maintenance system. This study is intended to propose the development plan for formation of positive flexibility culture in aircraft maintenance organization through the review of previous research on flexibility culture of organization and through empirical research for bureaucratic and poor factors by the evaluation of flexibility culture level of domestic aircraft maintenance organization.