Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Phenotype of Hepartocyte Spheroids in Synthetic Thermo-reversible Extracellular Matrix

  • Park, Keun--Hong (Center for Biomaterials and Biotechnology, Department of Materials sceience and Engineering, Kwangju Institute of Science and Technology) ;
  • Park, Ju-Young (Center for Biomaterials and Biotechnology, Department of Materials sceience and Engineering, Kwangju Institute of Science and Technology) ;
  • Bae, You-Han (Center for Biomaterials and Biotechnology, Department of Materials sceience and Engineering, Kwangju Institute of Science and Technology)
  • Published : 2001.07.01
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Abstract

Aggregates of specific cells are often regarded as better from in artificial organs and mammalian cell bioreactors in terms of cell-specific functionality. In this study, the morphology and liver specific functions of freshly harvested primary rat hepatocytes, which were cultivated as spheroids and entrapped in a synthetic thermo-reversible extracellular matrix, were examined and compared to a control (hepatocytes in single cell form). A copolymer of N-isopropylacrylamide(98 mole % in feed) and acrylic acid (poly (NiPAAm-co-AAC)), a thermo- reversible copolymer gel ma- trix, was used to entrap hepatocytes either in spheroids or single cells. During a 7-day culture pe-riod, the spheroids maintained higher viability and produced albumin and urea at a relatively con-stant rate, while, the single cell culture showed a slight increase in cell numbers and a reduction in albumin secretion Hepatocytes cultrured as spheroids present a potentially useful three-dimensional cell culture system for application in bioartificial liver device.

Keywords

References

  1. Proc. Natl. Acad. Sci. USA v.83 Survival, organization, and function of microcarrier-attached hepatocytes transplanted in rats. Demitrious, A. A.;S. M. Levenson;P. M. Novikoff;A. B. Novikoff;A. R. Chowdhury
  2. Hepatology v.21 Fulmination hepatic failure: Summary of a workshop. Hoofnagle, J. H.;R. L. Jr. Carithers;C. Shapiro;N. Ascher
  3. Hepatology v.21 In vivo evaluation of a hollow fiber liver assist device. Jauregui, H. O.;C. J. Mullon;D. Trenkler;S. Naik;H. Santangini
  4. Biomaterials v.11 Interacerebral implantation of synthetic polymer/biopolymer matrix: A new perspective for brain repair. Woerly, S.;R. Marchand;N. Lavallee
  5. Cellular solids: Structure and properties. King, G.;M. Ashby
  6. Exp. Cell Res. v.186 Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged and under other nonadherent environments. Koide, N.;K. Sakaguchi;Y. Koide;K. Asano;M. Kawaguchi;H. Matsushima;T. Takenami;U. Shinji;M. Mori;T. Tsuji
  7. J. Cell Biol. v.101 Spheroidal aggregate culture of rat liver cells: Histoypic reorganization, biomatrix deposition, and maintenance of functional activities. Landry, J.;D. Bernier;C. Ouellet;R. a Goyette;N. Marceau
  8. Biochem. Biophys. Res. Comm. v.161 Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver derived proteoglycans. Koide, N.;T. Shinji;T. Tanabe;K. Asano;M. Kawaguchi;K. Sakaguchi;Y. Koide;M, Mori;T. Tsuji
  9. Exp. Cell Res. v.189 Long-term culture of rat liver cell spheroids in hormonally defined media. Tong, J. Z.;O. Bernard;F. Alvarez
  10. J. Clin. Electron Microsc. v.22 Ultrastructure of multicellular spheroid formed in primary culture of adult rat hepatocytes. Asano, K.;N. Koide;T. Tsuji
  11. Cell Transplant. v.4 Formation of porcine hepatocyte spheroids for use in a bioartificial liver. Lazar, A.;H. J. Mann;R. P. Remmel;R. A. Shatford;F. B. Cerra, and W. S. Hu
  12. J. Control. Rel. v.53 Ex-tracellular matrix for a rechargeable cell delivery system. Bae, Y. H.;B. Vernon;C. K. Han;S. W. Kim
  13. J. Biomater. Sci. Polm. Eds. v.10 Insulin release from islets of Langerhans entrapped in a poly(N-isopropylacrylamide-co-acrylic acid) polymer gel. Vernon, B.;S. W. Kim;Y. H Bae
  14. Methods Cell Biol. v.13 Preparation of isolated rat liver cells. Seglen, P. O.
  15. Cytotechnology v.10 Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver. Nyberg, S.;L. R. A. Shatford;W. D. Payne;W. S. Hu;F. B. Cerra
  16. Biotechnol. Bioeng. v.50 Improvement of serum amino acid profile in hepatic failure with the bioartificial liver using multicellular hepatocyte spheroids. Shiraha, H.;N. Koide;H. Hada;K. Ujike;M. Nakamura;T. Shinji;S. Gotoh;T. Tsuji
  17. Clin. Chem. v.8 Modified reagents for determination of urea and ammonia. Chaney, A. L.;E. P. Marback
  18. Proc. Natl. Acad. Sci. USA v.80 Reciprocal modulation of growth and differentiated functions of mature rat hepatocytes in primary culture cell-cell contact and cell membrane. Nakamura, T.;K. Yoshimoto;Y. Nakayama