• Toxicological Research
• /
• 제18권2호
• /
• pp.167-174
• /
• 2002

Historical control data have been shown to be valuable in the proper interpretation and validation of reproductive toxicology studies. The present data were compiled from rat fertility and early embryonic development studies conducted at Korea Institute of Toxicology during the 1994∼2001 period. These data were assembled in order to provide background information for the general and reproductive data collected in 11 fertility and early embryonic development studies using Sprague-Dawley rats obtain-ing from the Breeding Facility, Korea Institute of Toxicology, Korea. A total of 274 males and 274 females were used in these studies during the eight-year period. Parameters of fertility and early embryonic development included clinical sign, body weights, food consumption, organ weights, estrus cycle, copulation index, precoital time, fertility index, pregnancy index, sperm parameters, and early embryonic development parameters. Most of the values were comparable to the previous historical control data reported by other investigators. These data can be wed not only as a historical data base for the meaningful interpretation of data from reproductive and developmental toxicity studies, but also as a contribution to biological characterization of Sprague-Dawley rats.

• Journal of Forest and Environmental Science
• /
• 제34권4호
• /
• pp.275-283
• /
• 2018

This study created the running water (Bandibooliwon) and lentic water (Sooryunwon) artificial habitats of Luciola lateralis in Hwadamsup, Gwangju-si, Gyunggi-do from April 2014 to February 2016. Through various habitat types, metapopulation was formed, and more sound population was induced to be maintained via interactions between habitats. We investigated coenosite at the habitat creation-target site, and 20 species in the lentic water site and 38 species in running water site were found. After releasing L. lateralis, we checked the status of settlement of L. lateralis larvae through traps and macroscopy, controlled the flow velocity of the habitats in line with the life cycle of L. lateralis (hatching, larva period, landing, pupation, copulation, and oviposition), and shaped gentle slope at water side, and planted moss. We artificially supplied melanian snails (Semisulcospira sp.), the food source, in the running water site and induced stable feeding. Also, We created artificial insectary, bred larvae stably in winter, and secured firefly population for a festival. Through night time observation, the time most suitable for a festival was confirmed to be from 20:30 to 22:00.

• Natural Product Sciences
• /
• 제4권2호
• /
• pp.58-61
• /
• 1998

Eurycoma longifolia Jack was evaluated for aphrodisiac property on sexually naive male mice using the electrical copulation cage. Optimum condition was provided for this study and the male mice were treated with 500 mg/kg of either chloroform, methanol, water or n-butanol fractions from E. longifolia Jack. However, the mice in the yohimbine and control groups received 30 mg/kg and 3 ml/kg of yohimbine and normal saline respectively. The male mice were then conditioned to seek either an estrous female, sexually vigorous male or no mouse, a measurement of right, wrong or no choice respectively. Besides this, hesitation time which was the time spent before the sexually naive male mice crossed the electrical grid (maintained at 0.12 mA) was also determined. Results showed that E. longifolia Jack possesses aphrodisiac property on the sexually naive male mice as shown by the slow and transient reduction in hesitation time and also a similar manner in the increase in the % of sexually naive male mice scoring right choice throughout the investigation period. Hence, this further supports the folkuse of this plant as aphrodisiac.

• 한국임상수의학회지
• /
• 제22권4호
• /
• pp.424-427
• /
• 2005

A 5-year-old Pekingese bitch was presented with a history of vaginal bleeding and copulation before 4-week. Ovarian remnant syndrome was diagnosed on the clinical changes, vaginal cytology and exploratory laparotomy. An abdominal mass was diagnosed on radiography, ultrasonography and Computed Tomography. The remnant of ovarian tissue and an abdominal mass were removed surgically under general anesthesia. Ultrasonographic examination of the removed ovarian tissue (water bath scanning) revealed three corpora lutea. The condition resolved following surgical excision of the remaining ovarian tissue and an abdominal purulent mass.

• 치과방사선
• /
• 제6권1호
• /
• pp.33-38
• /
• 1976

The author observed the effects of the cobalt-60 irradiation on the amelogenesis and dentinogenesis of the albino rat fetuses by means of histological and histochemical methods. Females in oestrus were mated overnight and examined the next morning for evidence of copulation. The lower left abdomen of mothers were exposed to cobalt-60 irradiation on the 10th day of gestation, l00R 200R and 300R respectively. The fetuses were removed from the mothers on the 18th day of gestation. The employed histochemical methods were PAS reaction, colloidal iron reaction, aldehyde fuchsin stain, α-amino acid reaction, -SH radical reaction and methyl- green pyronin stain. The results were as follows; 1. The group irradiated by l00R made no histological differences in comparison with the control group. 2. Increasing the irradiation to 200R, abnormal dentin formation occured, and resulted in enamel hypoplasia and in atrophy and necrosis of odontoblasts. In dentinal papilla, the dilation and the degeneration of the blood vessels, excessive reticular atrophy and osteodentin were revealed. 3. With the more irradiation (200R-300R), the positive material of PAS, α-amino acid and aldehyde fuchsin tended to decrease in the ameloblast and the odontoblast. No significant changes appeared in DNA, the stainability of methylgreen pyronin.

• Applied Biological Chemistry
• /
• 제32권1호
• /
• pp.57-63
• /
• 1989

담배저장해충은 숙성된 엽연초에 가장 심각한 해를 끼치는 것으로 알려져 있다. 이 해충의 성유인 물질들 중에서 serricornin이 가장 강한 생리활성을 보여주고 다른 유인물질들은 교접시 이것에 대한 보조역할을 한다. 분광학적인 증거와 합성에 관한 연구로부터 이 화합물의 구조는 (4S,6S,7S)-4,6-dimethyl-7-hydroxynonan-3-one임이 밝혀졌다. 담배저장 해충으로부터 얻어진 성유인 물질들은 모두 polyketide 생합성의 과정에서 생성된다고 제안되었다. 두 가지 nonasymmetric 합성과 열가지 asymmetric 합성이 상세하게 고찰되었다.

• 대한소아치과학회지
• /
• 제3권1호
• /
• pp.12-17
• /
• 1976

The study is about the effect of tetracycline-HCl on the amelogenesis and the dentinogenesis of the albino rats by means of histochemistry and fluorescence microscopy. Females in oestrus were mated overnight and examined the next morning for evidence of copulation. The mothers were intraperitonealy injected with a single dose of tetracycline-HCl from the eighth to tenth day of gestation. The heads of new born rats were fixed in Carnoy's solution and 10% formalin solution. The staining methods were alizarin red S stain, PAS reaction, colloidal iron reaction, Morin's stain and hematoxylin-eosin stain, The results were as follows: 1. By the single injection of tetracycline, the matrix formation of enamel and dentin were disturbed, and the shape and arrangement of ameloblast and odontoblast were distorted. 2. It seemed that, with the higher dose of tetracycline, the positive materials of PAS reaction were increased in the disturbed enamel and dentin matrix, but those of alizarin red S stain and colloidal iron reaction were decreased. 3. The fluorescence intensity in the disturbed enamel and dentin matrix were higher than the other areas and appeared to increase gradually with the higher dose of tetracycline.

• 한국수산과학회지
• /
• 제30권6호
• /
• pp.992-996
• /
• 1997

Study on sexual dimorphism in Littorphism in Littorina brevicula should be interesting to examine that how the species prevent niche-overlap between the sexes? and how the species act differently in the reproductive roles of the sexes? Sexual dimorphism on the shell of L. brevicula was examined with 211 periwinkles larger than 5 mm shell length in Pusan, Korea. Sexual dimorphism was detected in the aperture length only. Aperture length of the female was longer than that of the male. A difference in the sexual role in the reproduction seems to be a probable explanation for the sexual dimorphism in the aperture length. The reproductive role of the male is to choose the best female and to transfer the sperm successfully by copulation, and the role of the female is to spawn their fertilised eggs into the seawater successfully. Perhaps females migrate to lower elevations on the shore than the males, so that they are submerged when they spawn. Because wave action at the lower area of the shore is stranger than that at the higher area on the shore, the females might need larger aperture size than the males to avoid dislodgement.

• Molecules and Cells
• /
• 제39권7호
• /
• pp.573-579
• /
• 2016

During copulation, male Drosophila transfers Sex Peptide (SP) to females where it acts on internal sensory neurons expressing pickpocket (ppk). These neurons induce a post-mating response (PMR) that includes elevated egg-laying and refractoriness to re-mating. Exactly how ppk neurons regulate the different aspects of the PMR, however, remains unclear. Here, we identify a small subset of the ppk neurons which requires expression of a pre-mRNA splicing factor CG3542 for egg-laying, but not refractoriness to mating. We identify two CG3542-ppk expressing neurons that innervate the upper oviduct and appear to be responsible for normal egg-laying. Our results suggest specific subsets of the ppk neurons are responsible for each PMR component.

• 농업과학연구
• /
• 제49권3호
• /
• pp.451-462
• /
• 2022

Dioscorea bulbifera tubers contain several phytochemicals of pharmaceutical value. They have been traditionally used for treating various ailments, including postmenopausal symptoms. In the present study, we analyzed the direct effects of Dioscorea tuber extract on mouse spermatozoa. Its contraceptive effect was also evaluated by an intravaginal application before copulation. Mouse spermatozoa were cultured in vitro with various concentrations of the extract. After culturing, the spermatozoa were stained with fluorescein isothiocyanate peanut agglutinin or Coomassie blue to study the acrosome reaction, stained with trypan blue to study the viability, or treated with a hypo-osmotic medium to study the membrane damage. Estrous female mice were intravaginally injected with the extract and copulated with males. The extract induced acrosome exocytosis, viability loss, and membrane damage in a concentration-dependent manner. Female mice treated with the extract showed complete loss of fertility. These observations indicate that the Dioscorea bulbifera tuber extract could be used as a topical contraceptive. Infertility could be due to the precocious acrosome exocytosis of the spermatozoa or membrane damage.