• 제목/요약/키워드: Construction Death

검색결과 157건 처리시간 0.021초

ANALYSIS AND MANIPULATION OF CANDIDATE GENES FOR DIARRHEAL DISEASE VACCINE DEVELOPMENTS

  • Kim Young-Chang
    • 한국미생물학회:학술대회논문집
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    • 한국미생물학회 2000년도 International Meeting 2000
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    • pp.58-65
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    • 2000
  • Diarrheal diseases are a major cause of both illness and death in developing countries and are caused by rotavirus, Shigella spp., Salmonella spp., enterotoxigenic Escherichia coli (ETEC), and Vibrio spp. In this study, for the development of vaccine against diarrheal diseases caused by Shigella sonei, Salmonella typhimurium, E. coli O157, and Vibrio cholerae, cloning and nucleotide sequence analysis of genes and characteristics of their gene products in E. coli were performed. For construction of attenuated strain of S. sonnei KNIH104 and Salmonella typhimurium KNIH100, the aroA genes were cloned, respectively. The recombinant plasmid $_pJP{\Delta}A45$ containing aroA deleted region and suicide vector $(_pJP5603)$ was constructed. The aroA gene deleted mutants were constructed using this recombinant plasmid. For cloning gene encoding antigenic region of E. coli O157 KNIH317, the O-antigen synthesis gene cluster and sit gene was cloned. The E. coli XL1-Blue cells harboring this recombinant plasmid showed cytotoxicity in Vero cells. The ctx gene was cloned for tile purpose of antigenic region against V. cholerae KNIH002. Sequence analysis confirmed that the virulence gene cassette was consisted of ace, zot, ctxA and ctxB genes.

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르네상스 시기의 유토피아 문학에 나타난 도시.건축에 관한 연구 (A Study on the Urban & Architecture that were Described in Utopian Literature of Renaissance Period)

  • 이일형
    • 건축역사연구
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    • 제19권6호
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    • pp.79-98
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    • 2010
  • If we take account of the 'Architectural Tradition' which aims a construction of better environment, we can see that this tradition has ended historically toward Utopia. And the concrete concepts of utopia mainly started on the Renaissance periods. The Utopias were described well in the literatures which contained particularly three representative utopian books in Renaissance period. The one was the most famous novel by Sir Thomas More and the other were by Tommaso Campanella and by Francis Bacon. These novels expressed ideal commonwealths in which inhabitants exist under perfect conditions, ideally perfect places or state of things. The plans of utopia are complete projects of image, its goal is an political, social and economical improvement according to the eras. Their utopias mostly had characteristics as follows; their shape of islands were almost circle, their shape of cities were rectangularity or circle and attached importance to geometrical compositions, their structure of cities were self-sufficiency in closed spaces and their architectural characteristics were uniformity, simplicity and non-ornament. And these architectural characteristics are urban and architectural traditions in communist countries. Also their utopian novels had not much explanations to daily lives of people like as birth, death, relative, mental conflict or authority, money, art. So their utopian novels were not practical and had inappropriate aspects.

Slope Movement Detection using Ubiquitous Sensor Network

  • Jung, Hoon;Kim, Jung-Yoon;Chang, Ki-Tae;Jung, Chun-Suk
    • Journal of Electrical Engineering and Technology
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    • 제4권1호
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    • pp.143-148
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    • 2009
  • About 70% of Korea consists of mountainous areas, and during the construction of many roads and railroads, cut slopes are inevitably formed. The rainy season, frost heaving in winter, and thawing in spring can all cause rockfalls and landslides. The failure of these slopes is increasing every year, causing damage to vehicles, personal injury and even death. To protect people and property from such damage, a real-time monitoring system is needed to detect the early stages of slope failures. The GMG placed TRS sensor units in the slopes to monitor them in real-time. But due to its reliance on data lines and power lines, the system is vulnerable to lightning damage. The whole system can be damaged by a single lighting strike. Consequently, for the purposes of this paper we propose the use of the Ubiquitous Sensor Network (USN) which follows the IEEE 802.1.4. By using the USN system we can minimize lightning damage and can monitor the movement of the slopes consistently.

영유아 생명표 작성에 관한 연구 (Construction of a Life Table for Infant and Childhood)

  • 김종석
    • 한국인구학
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    • 제3권1호
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    • pp.52-61
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    • 1979
  • The purpose of the study is to construct a life table for population. It is based on the fraction of last year of life, $a_X$. The data necessary for this purpose have been obtained from the 1975 Population Census Report of Korea and the Vital Statistics of Shindong-myon, Chunseong-gun, Gangwon Province which is collected for the Seoul National University public health program. Age specific death rate is adapted by the Model Life Table, West, Level 21. For the age groups of less than 5 years of age, when the record of vital events can be obtained, the fractions are calculated from the community vital statistics. And for the age groups older than 5 years of age, Greville's Method is used. The findings are summarized as follows: 1) The fraction of last year of life in infantile group is 0.3684 for males, 0.3711 for females, and in 1-4 years of age group 1.2164 for males, 1.2274 for females. Both are more than those of Japan and U.S. in 1963. 2) Infant mortality rate is 42.37 for males, 31.77 for females per 1,000 live births. 3) The mortality curves show that a higher rate is observed under 1 year of age. It drops to the lowest point at around 10 years of age and rises again as the age increases. 4) The age estimated half-survival rate is during the age group of 70-74 for both sexes. 5) Life expectancy at the age of 0 shows 65.73 years for males and 69.22 years for females.

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The Role of Forensic Engineering in the Diagnosis of Electrocution Fatalities: Two Case Reports

  • Mohammad Alqassim;Raneem Ewiss;Hamdah Al Ali
    • Safety and Health at Work
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    • 제14권1호
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    • pp.124-130
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    • 2023
  • The increase in the number of fatal electric accidents over the years has escalated the demand for specialized forensic engineers to determine their relevant technical causes. Likewise, the complexities associated with identifying the causes of electrocution accidents have prompted the General Department of Forensic Science and Criminology at Dubai Police to adopt a new methodology to diagnose electrocution accidents, consisting of an approach that involves medico-legal examination, electrical diagnosis of the evidence, and trace evidence analysis. This paper will discuss the application of the adopted method in further detail by unfolding two case reports. The first report outlines a case in which a worker got electrocuted at a construction site while attempting to turn on a lamp. The second case report involves the death of a technician in a workshop after trying to disconnect a washing machine from its plug. The methodology was utilized during the investigation of both cases, which were attended by the appointed forensic engineers and showed promising results.

신규 고열 위험 업종 선정을 위한 우선순위 및 온열 위험 평가 (Prioritizing for Selection of New High-heat Risk Industries and Thermal Risk Assessment)

  • 신새미;이혜민;기노성;박정민;변상훈;김성호
    • 한국산업보건학회지
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    • 제33권2호
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    • pp.230-246
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    • 2023
  • Objectives: The climate crisis has arrived and heat-related illnesses are increasing. It is necessary to discover new high-heat risk industries and understand the environment . It is also necessary to prioritize risks of industries that have not been included in the management target to date. The study was intended to monitor and evaluate the thermal risk of high-priority workplaces. Methods: A prioritization method was developed based on five factors: occurrence of and death due to heat-related illnesses, work environment monitoring, indoor work rate, small heat source, and limited heat dissipation. it, was applied to industrial accidents caused by heat-related illnesses. Wet bulb temperature index and apparent temperature were measured in July and August at 24 workplaces in seven industries and assessed for thermal risk. Results: The wet bulb temperature index was in the range of 23.8~31.9℃, and exposure limits were exceeded in the growing of crops, food services activities and accommodation, and building construction. The apparent temperature was in the range of 26.8~36.7℃, and exceeded the temperature standard for issuing heatwave warnings in growing of crops, food services activities and accommodation, warehousing, welding, and building construction. Both temperature index in growing of crops and building construction were higher than the outside air temperature. Conclusions: In the workplace, risks in industries that have not be controlled and recognized through existing systems was identified. it is necessary to provide break times according to the work-rest time ratio required during dangerous time period.

노년기 배우자 사별 후 적응과정에서의 개인적 성장 (Personal Growth through Spousal Bereavement in Later Life)

  • 장수지
    • 한국사회복지학
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    • 제65권4호
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    • pp.165-193
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    • 2013
  • 본 연구의 목적은 노년기 배우자 사별 후의 성장과정을 살펴보고, 그에 대한 이론을 개발하는 것이다. 이를 위해 배우자와 사별한 노인 17명을 대상으로 질적 연구를 실시하였다. 심층면접 후 Strauss와 Corbin(1998)의 근거이론 방법을 적용하여 분석한 결과, 143개의 개념과 43개의 하위범주, 19개의 범주가 도출되었다. 배우자 사별 후 참여자들에게 나타난 중심현상은 '우울', '막막함', '생활상의 스트레스', '심리적 위축감', '회한', '홀가분함' 등의 심리적 반응이었다. 이러한 사별 직후의 심리적 손상 정도는 인과적 조건으로서 사별 전의 '부부관계', '개인의 독립적/의존적 성향', '사별에 대한 마음의 준비'에 따라 차이가 있었으며, 맥락적 조건에는 '친밀한 인간관계 구축에 대한 욕구', '독립성 유지에 대한 욕구'가 존재하였다. 현상을 극복하기 위한 작용/상호작용 전략은 '현실에 대한 직시'와 '새로운 삶을 위한 노력'이었으며, 이를 촉진, 제어하는 중재적 조건은 '사회적지지'와 '신앙생활'이었다. 나타난 결과, 즉 성장의 내용은 '삶의 의미 찾기', '자존감의 향상', '인간관계의 강화', '포용과 수용'이었다. 노년기 배우자 사별 후 성장의 과정은 시간 순에 따라 '슬픔과 절망단계', '끌어안고 나아가기 단계', '성장단계'로 이어졌다. 마지막으로 유형분석의 결과, 배우자 사별 후 성장과정은 '적극적 변화형', '발전적 적응형', '포용형', '답보형', '원망형'의 5가지로 분류되었다. 본 연구의 결과, 노년기 배우자 사별 후 성장은 노년기에 보편적으로 경험하게 되는 "발달적 위기 이후의 삶의 통합과정으로서, 배우자 사별 직후의 절망을 딛고 일어서 삶의 주체자로서 새로운 삶을 모색하고 더욱 강해진 자아를 발견해 나가며, 배우자를 포용해 나가는 과정"으로 개념화할 수 있다.

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VENGEANCE, VIOLENCE, VAMPIRES: Dark Humour in the Films of Park Chan-wook

  • Hughes, Jessica
    • 비교문화연구
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    • 제28권
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    • pp.17-36
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    • 2012
  • This essay places the South Korean film Thirst (2009) within Park Chan-wook's oeuvre as a filmmaker notorious for graphic depictions of violence and revenge. Park's use of dark humour in his films, which is emphasized in Thirst perhaps more than ever, allows for a more self-aware depiction of violence, where both the viewer and the protagonist are awakened to the futility of revenge. This ultimately paints his characters as fascinatingly crazy - simultaneously heroes, villains, and victims. Film theorist Wes D. Gehring's three themes of dark humour ('man as beast,' 'the absurdity of the world,' and 'the omnipresence of death') become most obvious in Park's most recent film, which pays closer attention to character development through narrative detail. Rather than portraying the characters as sentimental, dark humour depicts their misfortunes in an alternative way, allowing for consideration of such taboo subjects as religion, adultery, and death/suicide. These issues are further tackled through Thirst's portrayal of its vampire protagonist, which ultimately de-mystifies the traditional vampire figure. While this character has more often been associated with romance, exoticism and the mystical powers of the supernatural, Thirst takes relatively little from the demons of Nosferatu (Murnau, 1922) and various other Dracula adaptations, nor the romantic figures of Interview with the Vampire (Jordan, 1994), and Twilight (Hardwicke, 2008). Instead, it is part of a much smaller group of contemporary vampire films, which are rather informed by a postmodern reconfiguration of the monster. Thus, this paper examines Thirst as an important contribution to the global and hybrid nature of those films in which postmodern vampires are sympathetic and de-mystified, exhibiting symptoms stemming from a natural illness or misfortune. Park's undertaking of a vampire film allows for a complex balance between narrative and visuals through his focus on the Western implications of this myth within Korean cinema. This combination of international references and traditional Korean culture marks it as highly conscious of New Korean Cinema's focus on globalization. With Thirst, Park successfully unites familiar images of the vampire hunting and feeding, with more stylistically distinct, grotesque images of violence and revenge. In this sense, dark humour highlights the less charming aspects of the vampire struggling to survive, most effective in scenes depicting the protagonist feeding from his friend's IV in the hospital, and sitting in the sunlight, slowly turning to ash, in the final minutes of the film. The international appeal of Park's style, combining conventions of the horror/thriller genre with his own mixture of dark humour and non-linear narrative, is epitomized in Thirst, which underscores South Korea's growing global interest with its overt international framework. Furthermore, he portrayal of the vampire as a sympathetic figure allows for a shift away from the conventional focus on myth and the exotic, toward a renewed construction of the vampire in terms of its contribution to generic hybridization and cultural adaptation.

국립묘지 발전방안을 위한 추모 서비스 개선 연구 (Improvement of memorial service for national cemetery development plan)

  • 이정선
    • 서비스연구
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    • 제12권3호
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    • pp.134-143
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    • 2022
  • 국립묘지는 나라를 위해 목숨을 바친 분들의 뜻을 기리고 충의와 위훈을 영구히 추앙하기 위해 국가적 차원의 보훈을 실현하기 위한 공간이다. 국립묘지 설립의 목적은 국가와 민족을 위해 희생한 호국정신을 본받자는 것이다. 국가는 사적(私的)인 죽음과 국가를 위한 희생으로서의 공적(公的)인 죽음을 구분한다. 나아가 공적인 것으로 분류된 죽음을 세심하게 관리한다. 국립묘지는 바로 그러한 공적인 죽음을 관리하는 제도적 공간이다. 헌신과 훈격에 따른 예우로 안장 및 추모를 위한 기준이 신분 또는 계급 중심에서 벗어나야 한다. 국립묘지는 예우의 정도와 우선순위를 헌신 및 훈격 중심(重心)으로 전환하는 것이 민주 사회에서의 진정한 보훈 시스템이 될 것이다. 무엇보다도 먼저, 전사자와 더불어 국권 회복을 위해 헌신한 독립유공자들을 최고, 최우선 예우로 모실 때가 되었다. 이는 우리 국립묘지가 겪고 있는 갈등을 해소하고 국민의 공감을 얻는 첩경이다. 본 연구는 국립묘지가 국민으로부터 사랑받는 열린 추모 공간 및 역사 공간으로는 부족한 점이 있음을 전제로 출발하고 있으며, 국립묘지가 국민들로부터 사랑받는 열린 공간으로 거듭나도록 서비스 개선 중심으로 종합적인 발전방안을 제시하고자 한다, 또한 국내외 우수 사례와 고품격이면서도 친환경적인 선진국 국립묘지 조성사례를 통해 시사점과 적용 가능성을 검토한다.

Molecular phylogenetic relationships within the PSP producing marine dinoflagellate, genus Alexandrium

  • Kim, Choong-jae;Kim, Sook-Yang;Kim, Kui-Young;Kang, Young-Sil;Kim, Hak-Gyoon;Kim, Chang-Hoon
    • 한국양식학회:학술대회논문집
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    • 한국양식학회 2003년도 추계학술발표대회 논문요약집
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    • pp.136-137
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    • 2003
  • The marine dinoflagellate genus Alexandrium has been recognized as the most representative toxic phytoplankton on account of production of paralytic shellfish poisoning (PSP) throughout the world. PSP producers, generally A, tamarense and A. catenella, within the genus Alexandrium have caused high level intoxicauon of fisheries products and even death of human. In addition, more recent increasing of geographical range of this deleterious species has given rise to alarming tension. The study presented here aimed construction of the molecular phylogenetic relationships through sequences-determination from 16 morphotypic species (containing newly sequenced 3 morphotypic species, A. tamiyavainchii, A. fraterculus and A. pseudogonyaulax) in LSU rDNA D1-D2 and 12 morphotypic species (containing newly sequenced 6 - morphotypic species, A. catenella, A. tamiyavanichii, A. fraterculus, A. affine, A. insuetum and A. pseudogonyaulax) in SSU rDNA region, and the sequences were subjected to comparative-analysis in respect to regional population using functionally expressed rDNA genus and pseudogenes. And we discussed on genetic differentiation between A. tamarense and A. catenella together with putative PSP divegence of the genus Alexandrium. The results of phylogenetic analysis showed the robust monophyletic 14 distinct classes of A. tamarense, A. excavatum, A. catenella, Tasmanian A. tamarense, A. affine (and/or A. concavum), Thai A. tamarense, A. tamiyavanichii, A. fraterculus, A. margalefii, A. andersonii, A. ostenfeldii, A. minutum (and/or A. lusitanicum), A. insuetum, and A, pseudogonyaulx clade. A. fraterculus and A. tamiyavanichii were sister relationship and they were positioned independently between A, affine cluster and those of A. margalefi, A. andersonii, A. ostenfeldii, A. minutum and A. insuetum. A. pseudogonyaulax appeared to be an ancestral taxon among Alexandrium.

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