Proceedings of the Microbiological Society of Korea Conference (한국미생물학회:학술대회논문집)
- 2000.05a
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- Pages.58-65
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- 2000
ANALYSIS AND MANIPULATION OF CANDIDATE GENES FOR DIARRHEAL DISEASE VACCINE DEVELOPMENTS
Abstract
Diarrheal diseases are a major cause of both illness and death in developing countries and are caused by rotavirus, Shigella spp., Salmonella spp., enterotoxigenic Escherichia coli (ETEC), and Vibrio spp. In this study, for the development of vaccine against diarrheal diseases caused by Shigella sonei, Salmonella typhimurium, E. coli O157, and Vibrio cholerae, cloning and nucleotide sequence analysis of genes and characteristics of their gene products in E. coli were performed. For construction of attenuated strain of S. sonnei KNIH104 and Salmonella typhimurium KNIH100, the aroA genes were cloned, respectively. The recombinant plasmid
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