• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.922-929
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• 2019

Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3'-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3'-UTR and pGL3 Control LDLR mutant-3'-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine premiR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3'-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR3'-UTR-driven (p<0.01), but not mutant LDLR-3'-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = -0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3'-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.

• Journal of Korean Society of Rural Planning
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• v.13 no.3
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• pp.23-32
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• 2007

In Korea, a regulation of visual impact evaluation on development plan in natural environment is provided at the Natural Environment Conservation Act, but it was difficult to obtained the effectiveness enough to conserve natural landscape. So, the visual impact review on development plan is introduced to the act, through the revision of the Natural Environment Conservation Act in 2005. The basic directions of visual impact review are preservation, restoration, view protection, and harmony. The items of review are as follows; (1) development alternations for the provided plan, (2) executive reduction plan of visual impacts including consideration of growth of plants, (3) deliberation process between persons or parties concerned, etc. For rapid settlement of visual impact review system, it needs that the validity, the reliability, and the objectivity should be confirmed through steady research about reasonable guidance of review.

• Journal of Soil and Groundwater Environment
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• v.15 no.3
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• pp.1-6
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• 2010

This study investigated the change of metal contamination levels according to amendment of enforcement regulation of the Soil Environmental Conservation Act in Korea. As an analytical result of 87 samples in abandoned metallic mine area, the extracted amount of As, Pb and Cu with aqua regia was 4.3~29.6 times higher than that with hydrochloric acid extraction and the number of samples, which contamination levels were found to exceed soil contamination standards, was also increased. On the other hand, in case of Cd, Zn, and Ni, the number of samples, which contamination levels were found to exceed soil contamination standards, was decreased or similar. These results can be used as a preliminary material in comparison between the soil pollution data accumulated previously and the data obtained by the revised standard method for the examination of soil pollution.

• Molecules and Cells
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• v.28 no.2
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• pp.111-117
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• 2009

The CHRM3 gene is a member of the muscarinic acetylcholine receptor family that plays important roles in the regulation of fundamental physiological functions. The evolutionary mechanism of exon-acquisition and alternative splicing of the CHRM3 gene in relation to transposable elements (TEs) were analyzed using experimental approaches and in silico analysis. Five different transcript variants (T1, T2, T3, T3-1, and T4) derived from three distinct promoter regions (T1: L1HS, T2, T4: original, T3, T3-1: THE1C) were identified. A placenta (T1) and testis (T3 and T3-1)-dominated expression pattern appeared to be controlled by different TEs (L1HS and THE1C) that were integrated into the common ancestor genome during primate evolution. Remarkably, the T1 transcript was formed by the integration event of the human specific L1HS element. Among the 12 different brain regions, the brain stem, olfactory region, and cerebellum showed decreased expression patterns. Evolutionary analysis of splicing sites and alternative splicing suggested that the exon-acquisition event was determined by a selection and conservation mechanism. Furthermore, continuous integration events of transposable elements could produce lineage specific alternative transcripts by providing novel promoters and splicing sites. Taken together, exon-acquisition and alternative splicing events of CHRM3 genes were shown to have occurred through the continuous integration of transposable elements following conservation.

• Korean Journal of Heritage: History & Science
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• v.49 no.1
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• pp.178-189
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• 2016

This essay explores the ways in which the philosophical concept of "original form" is expressed in relevant laws and regulations, the legal character of respective regulations, the way in which each regulation is applied in practice for heritage management, and the factors required for this concept to serve as a legally binding fundamental principle. The current laws and regulations on heritage maintain a consistent requirement for preserving the original form of heritage, both for the general public and for heritage professionals. However, the principle of preserving original form is expressed as a declaration or imperative without substantive definitions. Consequently, heritage administrators simply follow administrative procedures for heritage conservation, management, and promotion while failing to specify the meaning of "original form." For the practical application of the principle of preserving original form to overall heritage conservation activities as an actual legal principle, further provisions should be added for the purpose of clarifying the principle, with consideration given to the observation of fundamental principles for legal provisions, such as the principles of clarity, equality, and proportion. The principle of preserving original form still functions as the most necessary principle for heritage conservation and therefore should be reestablished as a refined and rational regulatory system.

• Journal of the Korean Professional Engineers Association
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• v.16 no.1
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• pp.20-34
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• 1983

A phenomenon in the unplanned urban sprawl of Cheong-ju city has to be rest-rained from disposing and to develop, though progress in urbanization inevitably decreases rural area. So, this thesis aims at reviewing, revising and guiding toward the more effective land use planning practice and zoning mechanism and system of Cheong-ju city. I proposed that land use planning should be had several factors and thereupon that Korean zoning mechanism and system including Building Code should be amended because of inducing human activities in buildings and facilities not to demarcate and plan every one of them. First, the factors of urban planning are a rather diversified approach to it than a unified, supplementation of the applicable difference in time between preparing and appling it, a solid controlling system, a micro and macro planning theory and giving serious consideration for the inertia from the existing land use. Second, the applicable amendments for our zoning mechanism including Eluding Code are as follows; a need of reorganization for Zoning Ordinance appricated to different regulation concerning a scale and peculiarity of a oily, a decision of the different earmarked confinement in area to different zone ana from Zoning Ordinance in City Planning Law, a demarcated facilities and the more detailed land use concerning human activies than the exsisting and entrusting decision on the ways and processes of it to every rural city planning committee, a settlement of zone ana area considering for block-unit and Planned Unit Development(P. U. D.), and a need of security jurisprudence in order to solve the zoning regulation rather in City Planning Law than in Building Code that our zoning regulation and controlling system are depended on. According to these applicable amendments to Cheong-ju city, I proposed the conceptions of Cheong-ju city land use planning are as follows; a reinforcement with one cored pattern of urban land use, a selection of gradual urban sprawl by way of city development and renewal, a strengthening accessibility to the core of the city with Plurizing the system of arterial roads, a choice of priority to conservation at the core of tile city and security of open spaces and parking area at the area of the city, a harmonization between development and conservation at the inner ring area that is situated between the core and periphery reserved area in order to develop in the future, a buffered open space situated at the congested area with heterogenous functions, and a completion of urban open space system. The proposal made here so far is for the hope of the better structure of Cheong-ju city that should be granted to the peculiarity developed with educational, cultural, conservative and self-sufficient city functions.

• The Transactions of the Korean Institute of Power Electronics
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• v.12 no.6
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• pp.433-440
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• 2007

Recently, the reduction of standby power consumption is significantly issued in electronic and electrical industry for the conservation of environment. In the case of a switched mode power supply (SMPS), it is demanded high efficiency at extremely low output power conditions by consumers. However, it is very different from high efficiency techniques at full load conditions. In addition, many SMPSs are designed as a multi-output circuit for various loads because of cost down. This circuit is difficult to implement both high efficiency and good cross regulation performance, simultaneously. In this paper, secondary side post regulator (SSPR), current mode control method, and power sequence control technique are proposed to reduce standby power consumption and to improve cross regulation performance of the multi-output SMPSs which consist of single or multiple converter. The proposed methods are analyzed by their operational principles and optimal designs verified by experimental results with 110[W] and 270[W] SMPSs.

• Journal of Families and Better Life
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• v.16 no.3
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• pp.123-140
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• 1998

The purpose of this study was to investigate the consistency of socially responsible consumer attitudes and behaviors toward environment problem. The major findings were as follows; 1. The socially responsible consumer behaviors toward environment problem were classified into resource conservation purchasing & using in green product and recycling behavior by factor analysis. 2. Correlation coefficient of the socially responsible attitudes and behaviors toward environment problem was found to be above. 43. This result implies that the consistency of socially responsible attitudes and behaviors was very high. 3. In the area of total socially responsible consumer attitudes and behaviors toward environment problem the related variable on the consistency of attitude and behavior were altruism perceived power of big business liberalism alienation dogmatism perceived consumer effectiveness faith on the environmental regulation policy experience of environment education and consumer i formation.

• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.2
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• pp.105-122
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• 1994

The landscape plannings were composed of the fundamental provisions which are important factors for the city and local plannings in Germany. The State Government run institute like LOLF in NRW have been established to solve the serious ecological problems. The institute have completed the basic system covering various ecological problems, such as environmental affection analysis and ecological risk, to contribute the application of the various land-using plants. The ecological landscape plannings are regarded as major fundamental plans of environmental preservation for the plannings development. The problems about the local landscape plannings needed to be preserved ecologically are introduced into the Federal Nature Preservation Law and State Congress Law in order to carry out the environmental protection policy effectively. The local landscape plannings are divided into two-step models by the special classification system for the planning. One is build-up of an independent design separated from city plans functionally, and the other is a systematic regulation for cooperation between the local plans and ecological conservation functions. The local plannings including lands to be preserved as the Nature Preservatin Zone is classified a rest area total sectioned plains.

• Journal of the Korean Institute of Landscape Architecture
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• v.24 no.1
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• pp.42-54
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• 1996

Many of urban parks which have been designated for urban planning regulation are not well developed because the priority of investment is inferior to that of other public facilities. Park development is an urgent urban policy and it is hard to decide priority among parks. Therefore, it is necessary to evaluate the priority of park development. Thus the Analytic Hierarchy Process has been introduced because it is suitable for achieving objectivity by collecting the professional opinions. The goals of park development are evaluated by AHP method and the priorities among goals are conservation of natural environment, satisfying the residents' residents' needs, population density, regional pollution, land acquisition cost, ratio of greeneries and facilities within park areas. Priority of park development is decided by the standardization of each individual inventory data and considering of the professional opinions. Among the multi-attribute judgement methods, AHP method may be the most widely used. Despite the critical evaluations of the AHP, such as limitation of the nine-point scale and the rank reversal problem, application of AHP to decide the priority of park development is suitable for actual circumstances.