• Journal of Microbiology and Biotechnology
• /
• v.33 no.4
• /
• pp.471-484
• /
• 2023

Compost is widely used as an organic additive to improve the bioremediation of diesel-contaminated soil. In this study, the effects of compost amendment on the remediation performance, functional genes, and bacterial community are evaluated during the bioremediation of diesel-contaminated soils with various ratios of compost (0-20%, w/w). The study reveals that the diesel removal efficiency, soil enzyme (dehydrogenase and urease) activity, soil CH₄ oxidation potential, and soil N₂O reduction potential have a positive correlation with the compost amendment (p < 0.05). The ratios of denitrifying genes (nosZI, cnorB and qnorB) to 16S rRNA genes each show a positive correlation with compost amendment, whereas the ratio of the CH₄-oxidizing gene (pmoA) to the 16S rRNA genes shows a negative correlation. Interestingly, the genera Acidibacter, Blastochloris, Erythrobacter, Hyphomicrobium, Marinobacter, Parvibaculum, Pseudoxanthomonas, and Terrimonas are strongly associated with diesel degradation, and have a strong positive correlation with soil CH₄ oxidation potential. Meanwhile, the genera Atopostipes, Bacillus, Halomonas, Oblitimonas, Pusillimonas, Truepera, and Wenahouziangella are found to be strongly associated with soil N₂O reduction potential. These results provide useful data for developing technologies that improve diesel removal efficiency while minimizing greenhouse gas emissions in the bioremediation process of diesel-contaminated soil.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.12
• /
• pp.1993-2005
• /
• 2019

Thermophilic anaerobic digestion (TAD) is characterized by higher biogas production rates as a result of assumedly faster microbial metabolic conversion rates compared to mesophilic AD. It was hypothesized that the thermophilic microbiome with its lower diversity than the mesophilic one is more susceptible to disturbances introduced by alterations in the operating factors, as an example, the supply of nitrogen-rich feedstock such as poultry manure (PM). Laboratory scaled TAD experiments using cattle slurry and increasing amounts of PM were carried out to investigate the (in-) stability of the process performance caused by the accumulation of ammonium and ammonia with special emphasis on the microbial community structure and its dynamic variation. The results revealed that the moderate PM addition, i.e., 25% (vol/vol based on volatile substances) PM, resulted in a reorganization of the microbial community structure which was still working sufficiently. With 50% PM application, the microbial community was further stepwise re-organized and was able to compensate for the high cytotoxic ammonia contents only for a short time resulting in consequent process disturbance and final process failure. This study demonstrated the ability of the acclimated thermophilic microbial community to tolerate a certain amount of nitrogen-rich substrate.

• Journal of Korean Society of Rural Planning
• /
• v.22 no.1
• /
• pp.93-100
• /
• 2016

Land price can be affected by convenience or psychological repulsion like PIMFY (Please In My Front Yard) or NIMBY (Not In My Back Yard) for various facilities. Services related to public establishment, welfare, medical attention, and amenities in rural areas are comparatively poorer than those in urban areas. The purpose of this study is to estimate the implications of the accessibility to community facilities in rural areas for land prices using a hedonic price model. The accessibility to facilities is estimated by real road distances and the land prices are applied for four types of land usages: field, rice paddy, building lots, and village halls. Community facilities are classified from public and community services view: education, safety, culture, transport, environment, health care, and finance. The results show that the accessibility to health care and transport can positively affect land prices and the accessibility to environment (waste facilities and junkyard) and unpleasant services (funeral hall and charnel house) can negatively affect land prices. Especially, the accessibility to hospital is the most positive influential factor for all types of land usages.

• Journal of The Korean Association of Information Education
• /
• v.22 no.1
• /
• pp.159-167
• /
• 2018

In this study, we have developed an online learning community site using Humhub social network software and promote social constructive learning through the questions and answers in subject specific learning groups. By accumulating learning contents which consist of questions and answers about specific topics, learners can acquire knowledge by searching relevant topics and questions and can create and reconstruct knowledge as well as consuming knowledge by participating in self-regulated learning community. We have developed a mathematical editor feature which enables users to enter mathematical expression such as equations and greek characters. Online learning community sites can be used for inquiry based information education.

• Journal of Microbiology
• /
• v.41 no.4
• /
• pp.327-334
• /
• 2003

This study employed two PCR-based 16S rDNA approaches, amplified rDNA restriction analysis (ARDRA) and denaturing gradient gel electrophoresis (DGGE), to characterize the bacterial community structure in groundwater. Samples were collected from groundwater for the use by private residences, as well as for industrial and agricultural purposes, in Ansan City. Each PCR product was obtained by PCR with eubacteria 16S rDNA and variable V3 region specific primer sets. After amplification, the 16S rDNA PCR products were digested with 4-base site specific restriction endonucleases, and the restriction pattern analyzed. The genetic diversity and similarity of the groundwater bacterial community was analyzed by eubacteria universal primer sets for the amplification of variable V3 regions of the bacterial 16S rDNA. The result of the bacterial community analysis, by ARDRA and DGGE, revealed the same pattern. The highest diversity was found in groundwater from site G1, which was used in residences. In the DGGE profile, a high intensity band was sequenced, and revealed to be Pseudomonas sp. strain P51.

• Journal of Soil and Groundwater Environment
• /
• v.24 no.2
• /
• pp.23-37
• /
• 2019

To evaluate the available groundwater supply to the agricultural water demand in the future with the climate change scenarios for 40 sub-regions in Jeju Island, groundwater recharge and the available groundwater supply were estimated using water balance analysis method. Groundwater recharge was calculated by subtracting the actual evapotranspiration and direct runoff from the total amount of water resources and available groundwater supply was set at 43.6% from the ratio of the sustainable groundwater capacity to the groundwater recharge. According to the RCP 4.5 scenario, the available groundwater supply to the agricultural water demand is estimated to be insufficient in 2020 and 2025, especially in the western and eastern regions of the island. However, such a water shortage problem is alleviated in 2030. When applying the RCP 8.5 scenario, available groundwater supply can't meet the water demand over the entire decade.

• Journal of Ecology and Environment
• /
• v.35 no.2
• /
• pp.123-129
• /
• 2012

Salt marsh is an important transitional zone among terrestrial, riverine, and marine ecosystems and is a productive habitat that interacts extensively with adjacent landscape elements of estuarine and coastal ecosystems. Nowadays, in addition to various human activities, a variety of natural processes induce changes in salt marshes. This study aims to provide background information to restore disturbed salt marshes and to propose their ecological restoration using seed banks. The study area is a prepared area for the Gwangyang Container Port located in the southern Korea. This area was formed by accumulating mud soils dredged from the bottom of the forward sea. This land was created in a serial process of preparing the Gwangyang container port and the salt marsh was passively restored by seeds buried in mud soil dredged from seabed. As a result of stand ordination based on vegetation data collected from the land, stands were arranged according to tolerance to salinity in the order of $Suaeda$ $maritima$, $Salicornia$ $europaea$, and $Phragmites$ $communis$ communities on the Axis 1. Landscape structure of the projected area was analyzed as well. Edges of the projected area were divided from the marginal waterway by the dike. Four types of vegetation appeared on the dike: $Alnus$ $firma$ plantation, $Robinia$ $pseudoacacia$ plantation, $Lespedeza$ $cyrtobotrya$ plantation, and grassland. In the more internal areas, two types of vegetation sequences appeared: $Aster$ $tripolium$ community-$Suaeda$ $glauca$ community-$Salicornia$ $europaea$ community sequence and $Aster$ $tripolium$ community-$Suaeda$ $maritima$ community-$S.$ $europaea$ community sequence. Mixed community showed the highest species diversity (H' = 0.86) and $S.$ $europaea$ community showed the lowest (H' = 0.0). Evenness is the highest in Mixed community (J' = 2.26) and the lowest in $S.$ $maritime-S.$ $europaea$ community (J' = 0.0). Several plant communities were successfully established on the land created by mud soil dredged from the bottom of Gwangyang Bay. Moreover, community diversity in this area approached a similar level with those from other studies involving natural salt marshes. Therefore, restoration effect based on community diversity obtained in our study can be evaluated as a successful achievement. In this respect, although most salt marshes in Korea and other places worldwide have been destroyed or disturbed by excessive land use, feasibility of seed bank as a restoration tool is greatly expected.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.9 no.5
• /
• pp.478-495
• /
• 2016

The existing city planning was performed by internal few decision-maker, but the information that an individual could contact with through the evolution of the ICT technology increased in the smart city, and a channel diversified and came to be able to participate in a decision making process by various methods. I show that it is in the collaborative planning process to come to a mutual understanding with residents directly or indirectly if utilize big data in a process of the safe community planning of the smart city. Therefore, I compare the utilization contents between data of Matsubara-shi,Japan and data of certification city of Korea which received the certification of the WHO international safe community. In the area where this study prepares for the approval of the international safe community, it wants you to use it though you utilize the data as the supporting role of the residents participation plan.

• Korean Journal of Soil Science and Fertilizer
• /
• v.46 no.3
• /
• pp.173-181
• /
• 2013

Livestock wastes are considered as major environmental pollutants because they contain high concentration of organic materials. In 2001, The Environmental Department reported that stock farmers were increasing as 5.1%/year, which resulted in a gradual increase in livestock wastes generation. The direct disposal of livestock wastes create several environmental problems. Thus, several countries banned the disposal of livestock wastes in environment including aquatic systems. Recently, aeration-based liquid fertilization was considered as potential way for the disposal of livestock wastes. In this study, next generation sequencing (NGS) analysis was used to understand the microbial community changes during liquid fertilization of livestock wastes. Microbial community was compared with liquid fertilizer physicochemical analysis such as $BOD_5$, $COD_{Mn}$ pH, N (Nitrogen), P (Phosphorus), K (Potassium) etc. The physicochemical parameters and bacterial community results pave the way for producing effective livestock-based fertilizer. By comparing the physical characteristics of the manure with microbial community changes, it is possible to optimize the conditions for producing effective fertilizer.

• Journal of Korean Society of Rural Planning
• /
• v.26 no.2
• /
• pp.117-126
• /
• 2020

In this study, demands of smart technology development were analyzed for rural village communities. Questionnaire items were derived by grasping the current status of information and communication technology. 49 villages in 8 regions were selected and surveys and statistical analysis were conducted. The main results of the study are as follows. First, 92% of community leaders use smartphones, search for information (38%), communicate with the Internet (36%) using smartphones, use KakaoTalk (31%), and Facebook (24%). Second, in the rural and urban exchange activities, promote support information service (51%) and promote method suggestion service (48.5%) showed that the demand for services in promote field was high. It is linked to the creation of economic opportunities. Third, in the income and production activities, demand for distribution services technology (39.3%) was high in the field of production and distribution, and cold chains that help maintain freshness until food, such as meat, fish, and vegetables are delivered to consumers when agricultural products are distributed. The constant temperature control system needs to be actively introduced. Fourth, autonomy activities showed the highest demand for air conditioning and control systems (34.2%) of community building, and the lowest demand for electronic voting (9.4%) and videoconferencing (9.4%) services. Lastly, in the general activity area of the community, the demand for technology of emergency services (37.1%) and health self-diagnosis service (35.4%), which are technologies in the welfare sector, ranked first and second respectively.