• Journal of the Korean Society of Marine Environment & Safety
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• v.10 no.2 s.21
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• pp.81-88
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• 2004

We review the adaptation strategies of the 21st climate change in an application to sea level rise. For the development of appropriate adaptation strategies on the coast vulnerable to the sea level rise, we have to consider the issues such as where to adapt, how to adapt, and when to adapt. The coastal target needed adaptation can be found by the evaluation of adaptive capacity of the coastal zone which requires the understanding of impacts and adaptive potential of the natural and socioeconomic systems in the coastal zone. Planned adaptation options to sea level rise can be classified into three generic approaches as managed retreat, accommodation, and protection In practice, the implementation of the options requires the analysis of land use, degree of vulnerability, cost and benefit, etc, and may be combination of the options rather than one approach. In terms of the response timing, the adaptation can be grouped as anticipatory and reactive ones. Generally it is more effective to consider both anticipatory and reactive adaptations at the same time for the impacts of future sea level rise. Due to the scientific uncertainty of climate change issues including sea level rise, the adaptation processes have to be designed to deal with a series of processes such as information md awareness establishment, planning and design implementation, and monitoring and evaluation in continuity and long-term period.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.3
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• pp.1285-1290
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• 2014

The aim was to determine whether ultrasound targeted microbubble destruction (UTMD) promotes dual targeting of HSP72 and HSC70 for therapy of castration-resistant prostate cancer (CRPC), to improve the specific and efficient delivery of siRNA, to induce tumor cell specific apoptosis, and to find new therapeutic targets specific of CRPC.VCaP cells were transfected with siRNA oligonucleotides. HSP70, HSP90 and cleaved caspase-3 expression were determined by real-time quantitative polymerase chain reaction and Western blotting. Apoptosis and transfection efficiency were assessed by flow cytometry. Cell viability assays were used to evaluate safety. We found HSP72, HSC70 and HSP90 expression to be absent or weak in normal prostate epithelial cells (RWPE-1), but uniformly strong in prostate cancerous cells (VCaP). UTMD combined with dual targeting of HSP72 and HSC70 siRNA improve the efficiency of transfection, cell uptake of siRNA, downregulation of HSP70 and HSP90 expression in VCaP cells at the mRNA and protein level, and induction of extensive tumor-specific apoptosis. Cell counting kit-8 assays showed decreased cellular viability in the HSP72/HSC70-siRNA silenced group. These results suggest that the combination of UTMD with dual targeting HSP70 therapy for PCa may be most efficacious, providng a novel, reliable, non-invasive, safe targeted approach to improve the specific and efficient delivery of siRNA, and achieve maximal effects.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7621-7628
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• 2013

Human epidermal growth factor receptor (HER) status is an important prognostic factor in breast cancer. There is no globally accepted method for determining its status, and which method is most precise is still a matter of debate. We here analyzed HER2 mRNA expression by quantitative reverse transcription-PCR (qRT-PCR) and HER2 DNA amplification using multiplex ligation-dependent probe amplification (MLPA). In parallel, we performed a routine evaluation of HER2 protein by immunohistochemistry (IHC). To assess the accuracy of the RT-PCR and MLPA techniques, a combination of IHC and fluorescence in situ hybridization (FISH) was used, substituting FISH when the results of IHC were ambiguous (2+) and for those IHC results that disagreed with MLPA and qRT-PCR, this approach being termed IHC-FISH. The IHC results for four samples were not compatible with the MLPA and qRT-PCR results; the MLPA and qRT-PCR results for these samples were confirmed by FISH. The correlations between IHC-FISH and qRT-PCR or MLPA were 0.945 and 0.973, respectively. The ASCO/CAP guideline IHC/FISH correlation with MLPA was (0.827) and with RT-PCR was (0.854). The correlations between the IHC results (0, 1+ as negative, and 3+ as positive) and qRT-PCR and MLPA techniques were 0.743 and 0.831, respectively. Given the shortcomings of IHC analysis and greater correlations between MLPA, qRT-PCR, and FISH methods than IHC analysis alone with each of these three methods, we propose that MLPA and real-time PCR are good alternatives to IHC. However a suitable cut-off point for qRTPCR is a prerequisite for determining the exact status of HER2.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1181-1187
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• 2019

Today's sports, by themselves, express a wide variety of phenomena and reproduce or imply complex social symbols. The definition of sports being transformed in combination with ideology, which has become a central issue in each era, has become more diverse in recent years. Recently, with the 4th Industrial Revolution leading the social phenomenon, sports culture is producing a new phenomenon. In this era, we need the study in the question of how to understand and interpret sports culture is the right approach. The struggle for survival in each discipline was expressed as a reinterpretation of sports culture. This is to answer questions about how sports culture is consumed, spread and reinterpreted. The purpose of this study is to find out the direction and directing point of sports culture. Based on such problem recognition, five types of answers to what sports culture consumes were presented. Based on this, the fairness related to school sports, sports society-club(sports clubs), sports events, sports media, and sports was suggested as a medium for the spread of sports culture. We are accepting and transforming numerous scientific civilizations to improve sports culture and to promote consumers. However, there is a pity not to define such a thing. Efforts at a more fundamental level, such as cultural regulation and fundamental directions, need to be discussed. The framework of reinterpretation of sports culture should be constantly looking for directions and answers about what to do, not just the level of interpretation.

• Journal of Intelligence and Information Systems
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• v.15 no.4
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• pp.183-200
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• 2009

Collaborative Filtering is one of the most used recommender systems. However, basically it cannot be used to recommend new products to customers because it finds products only based on the purchasing history of each customer. In order to cope with this shortcoming, many researchers have proposed the hybrid recommender system, which is a combination of collaborative filtering and content-based filtering. Content-based filtering recommends the products whose attributes are similar to those of the products that the target customers prefer. However, the hybrid method is used only for the limited categories of products such as music and movie, which are the products whose attributes are easily extracted. Therefore it is essential to find a more effective approach to recommend to customers new products in any category. In this study, we propose a new recommendation method which applies centrality concept widely used to analyze the relational and structural characteristics in social network analysis. The new products are recommended to the customers who are highly likely to buy the products, based on the analysis of the relationships among products by using centrality. The recommendation process consists of following four steps; purchase similarity analysis, product network construction, centrality analysis, and new product recommendation. In order to evaluate the performance of this proposed method, sales data from H department store, one of the well.known department stores in Korea, is used.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.9
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• pp.1060-1068
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• 2007

Perchlorate contamination in aquatic system is a growing concern due to the human health and ecological risks associated with perchlorate exposure. In spite of potential risks associated with perchlorate, drinking water standard has not been established worldwide. Recently, US EPA has issued new protective guidance for cleaning up perchlorate contamination with a preliminary clean-up goal of 24.5 ppb. In Korea, the drinking water standard and discharge standard for perchlorate has not been established yet and little information is available to address perchlorate problems. Perchlorate treatment technologies include ion exchange, microbial reactor, carbon adsorption, composting, in situ bioremediation, permeable reactive barrier, phytoremediation, and membrane technology. The process description, capability, and advantage/disadvantages of each technology were described in detail in this review. One of recent trends in perchlorate treatment is the combination of available treatment options such as combined microbial reduction and permeable reactive burier. In this review, we provided a brief perspective on perchlorate treatment technology and to identify an efficient and cost-effective approach to manage perchlorate problem.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.244-249
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• 2009

At the 21th century management environment with the emotion and creativity, as design has recognized the core element of management strategy, the combination of design and management, design management, has been increased the importance worldwidely. In the rapid changing enterprise environment, the small or venture enterprises in flexible management environment than the large enterprise have advantage on management process. The design management has created the various field of knowledge in the integrated solution, the integrated approach methods of management are the effective operative process in the importance of design management. As CEOs in small/venture enterprise take part in the innovation of design management, a specialistes in recognition of specificity and design management are defined and created the design management process through the enterprise resource planning programs. With the understanding on the above situation, the purpose of this study is to suggest the design is strategically applied in the management level of Daejeon region small/venture enterprises and the recognition of design management as a strengthen the competitiveness.

• Journal of KIISE:Software and Applications
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• v.36 no.3
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• pp.187-199
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• 2009

With the advent of Web 2.0, OpenAPIs are becoming an increasing trend to emphasize Web as platform recently. OpenAPIs are used to combine services and generate new services by mashup. However because the standard documents for OpenAPIs do not exist, it may restrict the use of OpenAPIs. Previous studies of OpenAPIs mashup have been limited to tool design or language definition for service combination rather than dynamic composition. On the other hand, Web services that are a software technology implementing SOA provide standard documents such as WSDL to explain each service, UDDI to register it, and SOAP to transfer messages. Thus Web applications can interpret and execute services by using these technologies. Recent works have also been performed to provide semantic features and dynamic composition for SOA. If a dynamic and systematic approach is provided to combine Web services and OpenAPIs, Web applications can provide users with diverse services. In this study, we present a SOA based framework for mashup of OpenAPIs and Web services. The framework supports dynamic composition of OpenAPIs and Web services, where the process of composite services is described in OWL-S. A prototype is provided to validate our framework. The framework is expected to add diversity to typical Web services.

• Journal of Korea Water Resources Association
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• v.43 no.5
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• pp.495-505
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• 2010

The integrated basin-wide flood mitigation planning has a numerous individual proposals with different characteristics. So, it makes difficult to develop the optimal alternative that combines such elements. To develop the optimal alternative, it's necessary not only to assess the flood damage but also to define a tolerable degree of damage. Such a definition is made in a way, dubbed the 'social consensus' in a broad sense; but initially, it requires the analysis of the economic efficiency within a scope, which is technically achievable. This study aims to propose the alternatives development method so that the combination of the individual proposals could be economically efficient. To this end, this study defined the flood mitigation projects as the economic production activities carried out by inputs and outputs, and proposed the optimal project size as the principle of developing alternatives. This study may have the worth in improving the alternatives development method by considering the optimal project scale from the economic viewpoint while the existing method depending on engineering aspect. Such approach is expected to correspond to the current government's policy that avoids the passive flood mitigation focused on channel-wide planning but pursues the basin-wide flood mitigation planning focusing on damaged area, thereby provide efficient tools to come up with the solution.