• Title/Summary/Keyword: Combination Approach

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Immersion in sea cucumber's steroid extract to increase male production of juvenile freshwater crayfish

  • Gregorius Nugroho Susanto;Endang Linirin Widiastuti;Tri Rustanti;Sutopo Hadi
    • Fisheries and Aquatic Sciences
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    • v.26 no.1
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    • pp.48-57
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    • 2023
  • One of the ways to increase the production for aquaculture is through the cultivation of monosexuals by ensuring genital reversal from which energy for reproduction is diverted towards growth. Masculinization has been identified as one of the most prominent techniques, where sex development was directed from female to male. This approach only altered the phenotype and not the genotype. The red claw crayfish (Cherax quadricarinatus) was a relatively new commercial commodity, and the males were known to grow faster than females. Hence, it was proposed to use monocultures comprising an all-male population to increase yield using steroid hormone, synthetic 17α-methyltestosterone. However, this technique generated residues that detrimentally affect human health, the environment, and cultivated organisms. Therefore, finding new safe natural steroid sources was essential, and one of which is exploring of natural hormones extracted from the viscera of sea cucumbers (Holothuria scabra Jaeger). This study focused on the determination of male formation and testosterone levels among juvenile crayfish, after immersing in sea cucumber steroid extract (SCSE). A completely random design with factorial was used with two variables, encompassing the varied doses (0, 2, 4 mg/L, 2 mg/L 17α-methyl testosterone as control group) and immersion times of 18 and 30 h. The result showed the dose-dependent ability of SCSE increase the male genital formation and promote the testosterone level of juvenile crayfish. In addition, the testosterone was influenced by dose and immersion duration time, with the highest level of testosterone observed in treatments of 4 mg/L SCSE with 30 h immersion was 0.248 ng/mL, while the male percentage was 77%. In conclusion, the combination of dose and immersion time significantly affected growth and testosterone levels.

Grounded Theory Analysis on the Formation Process of Pro-environmental Behavior for Outdoor Recreation Participants (아웃도어 레크리에이션 참여자의 친환경행동 형성과정에 관한 근거 이론적 분석)

  • Kim, Bong-Ju;Park, Soo-Jung;Kim, Min-Kyu
    • 한국체육학회지인문사회과학편
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    • v.54 no.5
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    • pp.607-618
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    • 2015
  • This study aims to investigate overall factors regarding participation process such as process to pro-environmental behavior, pro-environmental behavior, continuing factors and constraint factors of outdoor recreation participants, and to determine logical correlation between them in order to make a theory model. To this end, grounded theory approach is applied for interpretation. First, pro-environmental behavior of outdoor recreation participants was formed by sequential influence of causal conditions and situational context. Second, outdoor recreation participants who participated in pro-environmental behavior of central phenomenon experienced peculiar continuing factors and constraint factors but it was found they also experienced general continuing factors and constraint factors. Third, it was analyzed that resultant settlement of pro-environmental leisure activities was partially fed back to the participation continuing factors of mediating conditions and a club of interaction strategy. Result from this study will give a help to widely understand experience of participation in leisure and restraints on leisure of outdoor recreation participants, and to development of study through organic combination between subsequent researches.

Short- and Long-Term Effects of Stock Split Disclosure: Exploring Determinants (주식분할 공시에 대한 장·단기 효과: 결정요인 분석을 중심으로)

  • Jin-Hwon Lee;Kyung-Soon Kim
    • Asia-Pacific Journal of Business
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    • v.14 no.1
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    • pp.73-91
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    • 2023
  • Purpose - The purpose of this study is to re-examine the disclosure effect of stock splits and long-term performance after stock splits using stock split data over the past 10 years, and infer the motivation (signal or opportunism) of stock splits. In addition, we focus on exploring the determinants of the short- and long-term market response to stock splits. Design/methodology/approach - We measure the short-term market response to a stock split and the long-term stock performance after the stock split announcement using the event study method. We analyze whether there is a difference in the long-term and short-term market response to a stock split according to various company characteristics through univariate analysis and regression analysis. Findings - In the case of the entire sample, a statistically significant positive excess return is observed on the stock split announcement date, and the excess return during the 24-month holding period after the stock split do not show a difference from zero. In particular, the difference between short-term and long-term returns on stock splits is larger in companies with a large stock split ratio, small companies, large growth potential, and companies with a combination of financial events after a stock split. Research implications or Originality - The results of this study suggest that at least the signal hypothesis for a stock split does not hold in the Korean stock market. On the other hand, it suggests that there is a possibility that a stock split can be abused by the manager's opportunistic motive, and that this opportunism can be discriminated depending on the size of the stock split, corporate characteristics, and financing plan.

Insight from sirtuins interactome: topological prominence and multifaceted roles of SIRT1 in modulating immunity, aging, and cancer

  • Nur Diyana Zulkifli;Nurulisa Zulkifle
    • Genomics & Informatics
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    • v.21 no.2
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    • pp.23.1-23.9
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    • 2023
  • The mammalian sirtuin family, consisting of SIRT1-SIRT7, plays a vital role in various biological processes, including cancer, diabetes, neurodegeneration, cardiovascular disease, cellular metabolism, and cellular homeostasis maintenance. Due to their involvement in these biological processes, modulating sirtuin activity seems promising to impact immuneand aging-related diseases, as well as cancer pathways. However, more understanding is required regarding the safety and efficacy of sirtuin-targeted therapies due to the complex regulatory mechanisms that govern their activity, particularly in the context of multiple targets. In this study, the interaction landscape of the sirtuin family was analyzed using a systems biology approach. A sirtuin protein-protein interaction network was built using the Cytoscape platform and analyzed using the NetworkAnalyzer and stringApp plugins. The result revealed the sirtuin family's association with numerous proteins that play diverse roles, suggesting a complex interplay between sirtuins and other proteins. Based on network topological and functional analysis, SIRT1 was identified as the most prominent among sirtuin family members, demonstrating that 25 of its protein partners are involved in cancer, 22 in innate immune response, and 29 in aging, with some being linked to a combination of two or more pathways. This study lays the foundation for the development of novel therapies that can target sirtuins with precision and efficacy. By illustrating the various interactions among the proteins in the sirtuin family, we have revealed the multifaceted roles of SIRT1 and provided a framework for their possible roles to be precisely understood, manipulated, and translated into therapeutics in the future.

A Study on the Effect and Determinants of Virtual Presence in Live Commerce: Focusing on the Characteristics of Live Shopping Media and Influencers (라이브커머스에서 가상실재감의 효과와 결정요인 연구: 라이브쇼핑 매체 및 인플루언서 특성을 중심으로)

  • Choi, Su Jeong;Kim, Tae Kyung
    • The Journal of Information Systems
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    • v.32 no.1
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    • pp.23-51
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    • 2023
  • Purpose: Live commerce is a new type of electronic commerce in combination with live streaming services. It is expected to increase virtual presence in the context of online shopping by overcoming a lack of social interactions between sellers and buyers which have been raised as a limitation in electronic commerce. Drawing on the studies of communication media, this study examines how live commerce contributes to the increase of virtual presence which consists of telepresence and social presence. Telepresence refers to a buyer's perception that he or she is present at the physical shopping mall during live shopping streaming whereas social presence refers to a buyer's perception of social interaction with a seller which is human warm, social, sensitive, and personal. In this study, we verify key determinants of virtual presence and its consequences. More specifically, this study proposes virtual presence contributes to the increase of buyers' trust in products and further purchase intentions. Furthermore, we verify influential factors of virtual presence from the technical and influencer perspectives of live commerce. Design/methodology/approach: To test the proposed hypotheses, the partial least squares (PLS) analysis is conducted with a total of 250 data collected on users with experience in the TaoBao live streaming shopping platform. Findings: The results show that first, telepresence and social presence are increased by visibility, media richness and attractiveness in the context of live shopping streaming. Second, buyers' trust in product trust and purchase intentions are positively influenced by telepresence and social presence. Finally, buyers' trust in product has a direct, positve effect on their purchase intentions. Overall, the findings offer new insights into the studies of electronic commerce by introducting the concepts of virtual presence and media richness from the literature of communication media in the field of live commerce.

Enhancing the Distribution of Community Enterprise Entrepreneurs through Product and Packaging Development of Klong Kone Shrimp Paste (Mesopodopsis) in the Post-Pandemic Era of Samut Songkhram Province

  • Panida NINAROON;Cholpassorn SITTHIWARONGCHAI;Chumpol RODJAM;Preecha KHAMMADEE;Pawintana CHAROENBOON
    • Journal of Distribution Science
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    • v.21 no.10
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    • pp.21-29
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    • 2023
  • Purpose: The current study focused on developing Klong Kone Shrimp Paste (Mesopodopsis) products and packaging, aiming to enhance the capabilities of entrepreneurs with cultural capital in post-pandemic Samut Songkhram Province. Research design, data and methodology: This study sought to analyze product and packaging issues and examine packaging expectations for Klong Kone shrimp paste. A combination of quantitative and qualitative methods was used, including a questionnaire administered to a sample of 400 individuals who purchased community enterprise products. Descriptive analysis, involving percentage, mean, and S.D., along with qualitative analysis through phenomenological methods, such as in-depth interviews and focus groups, were conducted with community enterprise entrepreneurs, experts, and customers. Results: The findings indicate that addressing product conditions and packaging issues with Klong Kone shrimp paste can be achieved by incorporating an attached label on the community product packaging. Conclusion: This approach is necessary due to the lack of community identity reflection and unattractive design, which currently deter customers from considering the products as souvenirs. Additionally, diversifying product offerings is recommended to better align with consumer needs. In terms of packaging expectations, a majority of respondents expressed a strong preference for an attractive appearance.

The Analyze the Effect of Home-based Integrative Korean Medicine Program for the Elderly Women with Mobility Impairment in Rural Areas at a Public Health Center (일개 보건지소의 농촌 거동 불편자 고령 여성 노인 대상 한방 방문진료의 효과 분석)

  • Chan-Ho Ju;Jin-Moo Lee;Chang-Hoon Lee;Jun-Bock Jang;Deok-Sang Hwang
    • The Journal of Korean Obstetrics and Gynecology
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    • v.36 no.4
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    • pp.49-60
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    • 2023
  • Objectives: To explore the impacts of home-based integrative Korean medicine program, on pain, quality of life (QOL), and satisfaction among elderly female residents with mobility impairment in rural areas during the period from August 2021 to November 2022. Methods: Five participants with mobility impairment in rural area were selected from Health Department of Public Health Center. We conducted an investigation into the variations in NRS (Numerical Rating Scale) scores, changes in quality of life (QOL), and levels of satisfaction resulting from a combination of acupuncture, counseling, and the provision of medical supplies. Results: Participants reported improvements in Overall pain and Quality of life. Satisfaction levels reported high. Conclusions: Home-based integrative Korean medicine program showed possibility of improvements for the elderly women with mobility impairment in rural areas in terms of pain, quality of life. In pursuit of improved management, it is advisable to contemplate the adoption of a fresh treatment approach.

Rapid Detection of Streptococcus mutans Using an Integrated Microfluidic System with Loop-Mediated Isothermal Amplification

  • Jingfu Wang;Jingyi Wang;Xin Chang;Jin Shang;Yuehui Wang;Qin Ma;Liangliang Shen
    • Journal of Microbiology and Biotechnology
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    • v.33 no.8
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    • pp.1101-1110
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    • 2023
  • Streptococcus mutans is the primary causative agent of caries, which is one of the most common human diseases. Thus, rapid and early detection of cariogenic bacteria is critical for its prevention. This study investigated the combination of loop-mediated isothermal amplification (LAMP) and microfluid technology to quantitatively detect S. mutans. A low-cost, rapid microfluidic chip using LAMP technology was developed to amplify and detect bacteria at 2.2-2.2 × 106 colony-forming units (CFU)/ml and its detection limits were compared to those of standard polymerase chain reaction. A visualization system was established to quantitatively determine the experimental results, and a functional relationship between the bacterial concentration and quantitative results was established. The detection limit of S. mutans using this microfluidic chip was 2.2 CFU/ml, which was lower than that of the standard approach. After quantification, the experimental results showed a good linear relationship with the concentration of S. mutans, thereby confirming the effectiveness and accuracy of the custom-made integrated LAMP microfluidic system for the detection of S. mutans. The microfluidic system described herein may represent a promising simple detection method for the specific and rapid testing of individuals at risk of caries.

Combinatorial Experiment for Al-6061 and Al-12Si alloy Based on Directed Energy Deposition (DED) Process (3차원 적층 제조 공정(DED) 기반 Al-6061+Al-12Si 합금 조합 실험)

  • Seoyeon Jeon;Suwon Park;Yongwook Song;Jiwon Park;Hyunyoung Park;Boram Lee;Hyunjoo Choi
    • Journal of Powder Materials
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    • v.30 no.6
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    • pp.463-469
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    • 2023
  • Aluminum alloys, known for their high strength-to-weight ratios and impressive electrical and thermal conductivities, are extensively used in numerous engineering sectors, such as aerospace, automotive, and construction. Recently, significant efforts have been made to develop novel aluminum alloys specifically tailored for additive manufacturing. These new alloys aim to provide an optimal balance between mechanical properties and thermal/electrical conductivities. In this study, nine combinatorial samples with various alloy compositions were fabricated using direct energy deposition (DED) additive manufacturing by adjusting the feeding speeds of Al6061 alloy and Al-12Si alloy powders. The effects of the alloying elements on the microstructure, electrical conductivity, and hardness were investigated. Generally, as the Si and Cu contents decreased, electrical conductivity increased and hardness decreased, exhibiting trade-off characteristics. However, electrical conductivity and hardness showed an optimal combination when the Si content was adjusted to below 4.5 wt%, which can sufficiently suppress the grain boundary segregation of the α-Si precipitates, and the Cu content was controlled to induce the formation of Al2Cu precipitates.

Checkmeat: A Review on the Applicability of Conventional Meat Authentication Techniques to Cultured Meat

  • Ermie Jr. Mariano;Da Young Lee;Seung Hyeon Yun;Juhyun Lee;Seung Yun Lee;Sun Jin Hur
    • Food Science of Animal Resources
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    • v.43 no.6
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    • pp.1055-1066
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    • 2023
  • The cultured meat industry is continuously evolving due to the collective efforts of cultured meat companies and academics worldwide. Though still technologically limited, recent reports of regulatory approvals for cultured meat companies have initiated the standards-based approach towards cultured meat production. Incidents of deception in the meat industry call for fool-proof authentication methods to ensure consumer safety, product quality, and traceability. The cultured meat industry is not exempt from the threats of food fraud. Meat authentication techniques based on DNA, protein, and metabolite fingerprints of animal meat species needs to be evaluated for their applicability to cultured meat. Technique-based categorization of cultured meat products could ease the identification of appropriate authentication methods. The combination of methods with high sensitivity and specificity is key to increasing the accuracy and precision of meat authentication. The identification of markers (both physical and biochemical) to differentiate conventional meat from cultured meat needs to be established to ensure overall product traceability. The current review briefly discusses some areas in the cultured meat industry that are vulnerable to food fraud. Specifically, it targets the current meat and meat product authentication tests to emphasize the need for ensuring the traceability of cultured meat.