• 제목/요약/키워드: Colony characteristics

검색결과 417건 처리시간 0.03초

Coniella granati에 의한 석류 열매썩음병 (Fruit Rot of Pomegranate (Punica granatum) Caused by Coniella granati in Korea)

  • 권진혁;박창석
    • 식물병연구
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    • 제8권4호
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    • pp.215-219
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    • 2002
  • 2002년 7월 경남 통영시 광도면 석류재배 농가포장에서 열매가 부패되는 이상증상이 발생하였다. 분리된 병원균은 감자한천배지에서 균총의 색깔은 흰색이며 물결모양을 이루면서 왕성하게 자랐고, 배지상에서 분생포자각을 잘 형성하였다. 분생포자는 단세포이며 모양은 방추형이고, 색깔은 갈색 또는 올리브색이다. 크기는 10.3~17.4$\times$2.8~4.0 $\mu\textrm{m}$ 였다. 분생포자 형성 세포는 짧은 원통형이며 무색으로 크기는 12.4~1.4$\times$2.8~3.6 $\mu\textrm{m}$ 였다. 불생포자각의 모양은 구형이며 갈색으로, 각벽은 2~3세포이고, 크기는 124.6~228.4 $\mu\textrm{m}$ 였다. 균사생육 적온은 $25^{\circ}C$였다. 분리된 병원균을 건전한 석류에 유상, 무상접종한 결과 접종 3일 후 감염을 일으켰으며, 병원성이 강하였다. 이상과 같이 이 병원균을 Coniella granati에 의한 석류열매 썩음병으로 명명할 것을 제안한다.

Sclerotinia trifoliorum에 의한 자운영 균핵병 (Sclerotinia Rot of Astragalus sinicus Caused by Sclerotinia trifoliorum)

  • 권진혁;이흥수;이용환;심홍식
    • 식물병연구
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    • 제16권1호
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    • pp.90-93
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    • 2010
  • 경남 고성군 자운영 재배포장에서 잎과 줄기가 수침상으로 무르고 썩으며, 시들어 말라 죽고 병든 부분에 흰색의 곰팡이와 균핵이 발생하였다. 감자한천배지 상에서 균총은 연한 회색이며 균핵의 모양은 구형이고 검은색으로 크기는 $2{\sim}14{\times}2{\sim}7mm$이었다. 균사 생육적온과 균핵 형성은 $20^{\circ}C$이었다. 자낭반은 구형 또는 원반 모양이며 크기는 3~8 mm이었다. 자낭은 원통형이고 크기는 $145{\sim}210{\times}10{\sim}12{\mu}m$이었다. 이상과 같이 자운영에 발생한 병징, 병원균의 균학적 특징 및 병원성 검정한 결과 Sclerotinia trifoliorum Eriksson에 의한 자운영 균핵병으로 동정하였다.

국내 일부학교 건축물의 실내공기질 평가 (The Assessment of Survey on the Indoor Air Quality at Schools in Korea)

  • 손종렬;노영만;손부순
    • 한국환경보건학회지
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    • 제32권2호
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    • pp.140-148
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    • 2006
  • Recently, indoor air quality (IAQ) in workplace, residential environments and schools has been concerned of people, scientists and related the public, and has recognized the health effects related to indoor air pollution. Therefore, this study was performed to investigate the characteristics of IAQ in 55 kindergartens, elementary school, middle schools, and high schools from June, 2004 to May, 2005 in Korea. We measured indoor air pollutants($PM_{10},\;CO_2$, HCHO, total bacteria colony(TBC), CO, radon, TVOCs, asbestos, and $O_3$), and physical factors(noise, temperature, relative humidity, and illumination) with necessary of management for IAQ in school. We classified into 5 kinds of the school by period since building completion, <1 year, 1-3 years, 3-5 years, and 5-10 years. The concentration of pollutants and the level of physical factors compared with standards and guidelines of IAQ on the Ministry of Environment, the Ministry of Health and Welfare, and the Ministry of Education and Human Resources Development. The major results obtained from this study were as follows. Temperature, relative humidity and illumination among the physical factors did not exceed the standards, but noise exceeded it. Asbestos and $O_3$ did not detect in surveyed classrooms. CO, TBC, TVOCs, and HCHO in kindergartens, TBC in elementary schools, TBC, TVOCs dnd HCHO in middle schools, and HCHO in high schools detected the standards. This study is conducted as a part of efforts to provide a foundational data for further relative researches on management of IAQ of school. Therefore, we suggest that country plan for management of IAQ in school should be established through long-term and continuous investigation for assessment on IAQ in school and health risk assessment for students.

토양으로부터의 Agrobacterium tumefaciens의 분리, 동정 및 옥수수의 형질전환에 이용 (Identification of Agrobacterium tumefaciens from Soil and Transformation of Maize)

  • 노광수;강봉중
    • KSBB Journal
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    • 제7권3호
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    • pp.191-200
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    • 1992
  • 토양 시료로부터A. tumefaciens를 분리 동정하고 이를 옥수수의 형질전환에 이용하기 위하여 binary vector를 이용하여 형질전환시킨 후 옥수수의 중배축 조직과 공존배양을 실시하였다. Schroth의 선택배지에서 선별된 colony들은 단일 형태를 나타내었다. 분리 균주는 해바라기 유식물의 잎조직에 종양을 형성하였으며, 거대 plasmid를 가지고 있었는데 이는 대조균주인 C58이나 Ach5의 plasmid와는 다른 종류였다. 분리균주들에 의해 형성된 tumor의 형태 및 균주의 생리, 생화학적 특성들에 의하여 공시균주를 A. tumefaciens biovar 1으로 동정하였으며 nopaline 형으로 추정하였다. 분리 균주중 AK204를 사용하여 옥수수 조직을 형질전환시키기 위하여 선택maker를 가지고 있는 binary vector(pGA642)를 균주내로 전이 시켰다. 이로 인하여 형질전환된 AK204를 옥수수 중배축 조직과 공존배양하므로서 옥수수 조직세포를 형질전환 시킨 결과, 1차적으로 Km에 저항성을 띠는 형질전환체를 선발할 수 있었으며, 그들의 가용성 단백질의 PAGE pattern이 변화되었음을 확인하였다.

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Involvement of Cdc25c in Cell Cycle Alteration of a Radioresistant Lung Cancer Cell Line Established with Fractionated Ionizing Radiation

  • Li, Jie;Yang, Chun-Xu;Mei, Zi-Jie;Chen, Jing;Zhang, Shi-Min;Sun, Shao-Xing;Zhou, Fu-Xiang;Zhou, Yun-Feng;Xie, Cong-Hua
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권10호
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    • pp.5725-5730
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    • 2013
  • Cancer patients often suffer from local tumor recurrence after radiation therapy. Cell cycling, an intricate sequence of events which guarantees high genomic fidelity, has been suggested to affect DNA damage responses and eventual radioresistant characteristics of cancer cells. Here, we established a radioresistant lung cancer cell line, A549R, by exposing the parental A549 cells to repeated ${\gamma}$-ray irradiation with a total dose of 60 Gy. The radiosensitivity of A549 and A549R was confirmed using colony formation assays. We then focused on examination of the cell cycle distribution between A549 and A549R and found that the proportion of cells in the radioresistant S phase increased, whereas that in the radiosensitive G1 phase decreased. When A549 and A549R cells were exposed to 4 Gy irradiation the total differences in cell cycle redistribution suggested that G2-M cell cycle arrest plays a predominant role in mediating radioresistance. In order to further explore the possible mechanisms behind the cell cycle related radioresistance, we examined the expression of Cdc25 proteins which orchestrate cell cycle transitions. The results showed that expression of Cdc25c increased accompanied by the decrease of Cdc25a and we proposed that the quantity of Cdc25c, rather than activated Cdc25c or Cdc25a, determines the radioresistance of cells.

Rhizopus stolonifer에 의한 바나나 무름병 발생 (Occurrence of Rhizopus Soft Rot on Musa sapientum Caused by Rhizopus stolonifer in Korea)

  • 권진혁
    • 한국균학회지
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    • 제35권1호
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    • pp.54-57
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    • 2007
  • 2005년부터 2006년까지 2년 동안 경남 진주시 농산물 도매시장에 유통 판매중인 바나나에서 이상증상이 발생하였다. 병징은 상처난 부위의 과실이 수침상으로 물러지면서 검은색으로 빠르게 부패되었다. 균총은 처음 흰색에서 연한 갈색으로 되며 검은색의 포자낭을 많이 형성하며 균사생육 적온은 $30^{\circ}C$였다. 포자낭경은 처음 흰색에서 연한 회색을 나타내며 폭은 $12{\sim}26\;{\mu}m$이었다. 포자낭은 처음 흰색에서 검은색으로 되며 모양은 구형 또는 반구형이며 크기는 $83{\sim}195\;{\mu}m$이었다. 주축은 반구형이며 크기는 $72{\sim}125\;{\mu}m$이었다. 포자낭포자는 담갈색으로 단포이며 구형 또는 타원형으로 불규칙한 것이 많으며 크기는 $9{\sim}20{\times}7{\sim}8\;{\mu}m$이었다. 이상과 같이 바나나에 발생한 병징과 병원균의 균학적 특징 및 병원성을 검정한 결과, 본 병해를 Rhizopus stolonifer에 의한 바나나 무름병으로 명명할 것을 제안한다.

Rhizopus stolonifer에 의한 멜론무름병 발생 (Occurrence of Rhizopus Soft Rot on Melon (Cucumis melo L.) Caused by Rhizopus stolonifer in Korea)

  • 권진혁
    • 한국균학회지
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    • 제36권2호
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    • pp.199-202
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    • 2008
  • 시장에서 판매중인 멜론에서 이상증상이 발생하였다. 병징은 과실의 상처난 부위에 수침상으로 무르면서 빠르게 부패되었다. 균총은 처음 흰색에서 연한 갈색으로 되고 검은색의 포자낭을 많이 형성하며 균사생육 적온은 $30^{\circ}C$였다. 포자낭은 처음 흰색에서 성숙하면 검은색으로 되고 구형 또는 반구형이며 크기는 $80{\sim}195\;{\mu}m$이었다. 포자낭경은 폭은 $12{\sim}24\;{\mu}m$이었다. 주축은 반구형이며 크기는 $70{\sim}90\;{\mu}m$이었다. 포자낭포자는 담갈색, 단포이며 구형 또는 타원형이며 크기는 $9{\sim}20\;{\times}\;6{\sim}8\;{\mu}m$이었다. 균학적 특성을 기초로 병원균은 Rhizopus stolonifer로 동정되었고 병원성이 확인되었다. 따라서 본 병해를 Rhizopus stolonifer에 의한 멜론 무름병으로 명명할 것을 제안한다.

A report of 39 unrecorded bacterial species in Korea, belonging to the Betaproteobacteria and Gammaproteobacteria

  • Choi, Ahyoung;Bae, Jin-Woo;Cha, Chang-Jun;Chun, Jongsik;Im, Wan-Taek;Jahng, Kwang Yeop;Jeon, Che Ok;Joh, Kiseong;Kim, Seung Bum;Seong, Chi Nam;Yoon, Jung-Hoon;Cho, Jang-Cheon
    • Journal of Species Research
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    • 제4권2호
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    • pp.109-126
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    • 2015
  • As a subset study to discover indigenous prokaryotic species in Korea, a total of 39 bacterial strains assigned to the classes Betaproteobacteria and Gammaproteobacteria were isolated from diverse environmental samples collected from soil, tidal flat, freshwater, seawater, seaweed, wetland, plant roots, guts of insects, and fermented foods. From the high 16S rRNA gene sequence similarity (>99.1%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 39 species have been described in Korea; therefore 4 species of 4 genera in the order Burkholderiales and 1 species in the order Neisseriales within the class Betaproteobacteria, and 10 species of 6 genera in the order Alteromonadales, 11 species of 3 genera in the order Pseudomonadales, 4 species of 4 genera in the order Enterobacteriales, 2 species of 2 genera in the order Vibrionales, 1 species in the order Aeromonadales, 3 species of 3 genera in the order Oceanospirillales, 2 species of 2 genera in the order Xanthomonadales, and 1 species in the order Chromatiales within the Gammaproteobacteia are reported for proteobacterial species found in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

A report of 29 unrecorded bacterial species in Korea, belonging to the Alphaproteobacteria

  • Liu, Qingmei;Kim, Seung-Bum;Cho, Jang-Cheon;Yoon, Jung-Hoon;Joh, Ki-seong;Cha, Chang-Jun;Chun, Jong-sik;Seong, Chi-Nam;Bae, Jin-Woo;Jahng, Kwang-Yeop;Jeon, Che-Ok;Im, Wan-Taek
    • Journal of Species Research
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    • 제4권2호
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    • pp.97-108
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    • 2015
  • As a subset study to discover indigenous prokaryotic species in Korea, a total of 29 bacterial strains assigned to the classes Alphaproteobacteria were isolated from various environmental samples collected from plant root, ginseng soil, forest soil, marsh, mud flat, freshwater and seawater. From the high 16S rRNA gene sequence similarity (>99.1%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 29 species included in Alphaproteobacteria is have been described in Korea; therefore 14 species of 9 genera in the order Rhizobiales, 7 species of 6 genera in the order Sphingomonadales and 4 species of 2 genera in the order Caulobacterales and 3 species in the order Rhodobacterales and 1 species in the order Rhodospirillales found in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

A report on 15 unrecorded bacterial species of Korea isolated in 2016, belonging to the class Betaproteobacteria

  • Kim, Dong-Uk;Seong, Chi-Nam;Jahng, Kwangyeop;Lee, Soon Dong;Cha, Chang-Jun;Joh, Kiseong;Jeon, Che Ok;Kim, Seung-Bum;Kim, Myung Kyum
    • Journal of Species Research
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    • 제7권2호
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    • pp.97-103
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    • 2018
  • In 2016, as a subset study to discover indigenous prokaryotic species in Korea, a total of 15 bacterial strains were isolated and assigned to the class Betaproteobacteria. From the high 16S rRNA gene sequence similarity (>98.8%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 15 species have been described in Korea; therefore, 1 strain of the Aquitalea, 5 strains of the Paraburkholderia, 2 strains of the Comamonas, 1 strain of the Cupriavidus, 1 strain of the Diaphorobacter, 2 strains of the Hydrogenophaga, 1 strain of the Iodobacter, 1 strain of the Massilia and 1 strain of the Rhodoferax within the Betaproteobacteria are described for unreported bacterial species in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.