• 대한한의학방제학회지
• /
• 제22권1호
• /
• pp.13-32
• /
• 2014

Objectives : Until now the study of Cnidii Rhizoma, hemorrhage, brain waves, such as ischemic brain injury, analgesic, effect overcome of the stress from pregnancy melanin formation and inhibiting effects skin whitening have been published regarding this article. Cnidii Rhizoma demonstrates its different abilities depending on the characteristics. This paper reported that effect of Cnidii Rhizoma in Dongeuibogam blended prescriptions as main medicine. In addition, by analyzing data, we studied about utilizing of Cnidii Rhizoma. Methods : Cnidii Rhizoma in Dongeuibogam Prescriptions as the main ingredient was built with database of 202 prescriptions. Thus analyzed data was summarized in detail.(Table-1) If there is no difference in the title of the prescription but in other case the configuration information is different, formulations 1 and 2 were divided by the table. Results : The following results were reached through investigations on the prescriptions usikng Cnidii Rhizoma as a key component. 1. Prescriptions taking Cnidii Rhizoma as a monarch drug are utilized for 40 therapeutic purposes. In particular, 12.3% of prescriptions appear in the chapter of head, and 10.8% of those appear in the chapter of women, and 9.4% of eye, 8.9% of child, 6.4% of wind disease respectively. 2. Prescriptions utilizing Cnidii Rhizoma as the main ingredient are used in the treatment of headache, dizziness and pregnancy hemorrhage fetal movement, premature birth and they are also used for treating 131 different types of disease. 3. The dosage of Cnidii Rhizoma in formulas is from 2pun(about 0.75g) to 5don(nearly 18.75g), however 1don(nearly 3.75g) has been taken the most for clinical application. 4. We find out that according to herbs or prescriptions, Cnidii Rhizoma has a variety of functions such as ascending & descending of energy. Samultang is the most useful base prescription which used the Cnidii Rhizoma as the main component. Conclusion : These results suggest that, Cnidii Rhizoma once-amount use (don nearly 3.75g) 4g in head, gynecology, ophthalmology, pediatrics and paralysis disease associated with oriental medicine resource development can be considered to be widely used These results suggest that Cnidii Rhizoma was used most with 1 don(4g) and can be widely used for the resource development to the disease such as brain, gynecology, ophthalmologhy, pediatrics and wind-associated symptoms.

• 동아시아식생활학회지
• /
• 제18권4호
• /
• pp.618-623
• /
• 2008

기능성 식품 및 기능성 화장품으로 활용 가능한 소재를 개발하기 위한 목적으로 자외선 차단 효과, 항산화 효과, 미백효과 등을 측정한 결과는 다음과 같다. 1. 자외선 차단 효과는 두충 추출물은 $280{\sim}210\;nm$에서 단삼은 $270{\sim}220\;nm$에서 나타내는 것으로 보아 자외선 차단용 화장품 원료로 사용 시 UV-C영역의 자외선을 흡수하는 목적으로 사용할 수 있을 것이며, 진피는 $400{\sim}210\;nm$에서 천궁은 $380{\sim}220nm$의 거의 자외선 파장의 전 영역에서 흡수를 나타내었다. 2. DPPH 라디칼에 대한 소거능은 천궁 추출물은 79.0%, 두충 추출물은 74.2%, 진피 추출물은 40.4%, 단삼 추출물은 19.4%의 저해율 순서로 천궁 추출물이 가장 높은 전자공여능을 나타내었다. 3. Superoxide dismutase(SOD) 유사활성율은 단삼 추출물은 21.9%, 진피 추출물은 18.3%, 두충 추출물은 11.7% 순으로 나타내었으나, 천궁 추출물은 반응 과정에서 부유물이 생성되어 정확한 값을 측정할 수 없었다. 4. Xanthine oxidase 저해 활성을 측정한 결과, 단삼 추출물은 79.3%, 천궁 추출물은 71.9%, 두충 추출물은 57.5% 순으로 xanthine oxidase 저해 활성도가 매우 높은 것으로 나타났다. 5. Tyrosinase 저해 활성도는 천궁 추출물은 59.5%, 단삼추출물은 22.8%, 진피 추출물은 27.5%, 두충 추출물은 12.4% 순으로 나타내었다. 이상의 결과를 보면 자외선 차단 효과는 진피와 천궁 추출물이 좋은 자외선 차단용 원료로 사용할 수 있을 것이며, 항산화 효과는 네 가지 추출물의 특성을 고려하여 적당히 혼합한 혼합물, 미백 효과는 천궁 추출물을 사용하면 좋은 기능성 식품 및 기능성 화장품 원료로 사용 가능한 것으로 사료된다.

• 대한소아치과학회지
• /
• 제32권1호
• /
• pp.55-66
• /
• 2005

천궁의 메탄올 수용액 추출물의 진정 작용을 밝히기 위하여 staircase test를 시행하였으며, 근육이완 효과를 검색하기 위하여 rotarod test를 시행하였다. 진정 작용의 기전 구명을 위하여 GABA 및 glycine 수용체에 작용하는 약물인 picrotoxin, yohimbine, isoniazid, strychnine 등의 약물을 이용하여 발작 유도에 걸리는 시간을 비교 관찰하였다. 또한 생체에서의 안전성을 검증하기 위하여 Irwin의 행동시험 및 신경세포에 대한 독성을 평가하기 위하여 MTT test를 실시하여 다음의 결과를 얻었다. 1. Staircase 실험에서 천궁 추출물을 10mg/kg 용량으로 경구 투여했을 때 climbing수는 대조군과 차이가 없었으나, rearing수에서는 대조군에 비하여 42%의 감소를 보였다. 2. 30mg/kg의 용량으로 경구 투여했을 때, climbing수는 대조군과 차이가 없었으나, rearing수에서는 51%의 감소를 유발하였다. 3. 근육이완 작용을 탐색하기 위한 rotarod 실험에서는 대조군과 차이를 나타내지 않았다. 4. 천궁 추출물의 투여는 picrotoxin, isoniazid, yohimbine에 의한 발작에서는 큰 변화를 유발하지 않았으나, strychnine에 의한 발작 유도시간은 41%의 증가를 유발하였다. 5. 일반 행동에서도 특이한 변화를 유발하지 않았으며, 신경세포에 적용했을 때 특이한 독성 반응을 나타내지 않았다. 천궁의 메탄을 수용액 추출물은 현저한 진정 작용을 유발하며, 그 작용 기전으로서 glycine의 작용을 증가시켜 chloride ion의 세포내 유입으로 인한 억제성 신경전도를 유도한다고 사료된다. 또한 근육이완 작용도 나타나지 않았으며, 특이한 생체독성도 유발되지 않은 것으로 보아, 향후 치과임상에서 진정요법의 한 수단으로서 사용될 수 있다는 가능성을 제시한다.

• 동의생리병리학회지
• /
• 제19권4호
• /
• pp.938-944
• /
• 2005

Down-regulation of melanin synthesis is required for recovery of pigmentary disorders and it is known that direct inhibitors of tyrosinase suppress melanin synthesis. We screened several oriental medicinal plants using B16/Fl0 cells and found that the methanolic extract of Cnidii Rhizoma down-regulated melanin synthesis effectively. Although the proliferation of Bl6/Fl0 cells was decresed by the methanolic extract of Cnidii Rhizoma, it did not appear necrosis. Bl6/F10 cells incubated with the methanolic extract of Cnidii Rhizoma showed reduced pigmentation and tyrosinase activity. Western blotting revealed that the amount of tyrosinase was decreased by the methanolic extract of Cnidii Rhizoma. These results suggest that the inhibitary effect of the methanolic extract of Cnidii Rhizoma on melanogenesis is due to the suppression of tyrosinase in Bl6/F10 cells and Cnidii Rhizoma is a candidate for an efficient whitening agent.

• 대한한의학방제학회지
• /
• 제18권2호
• /
• pp.25-51
• /
• 2010

This report describes 214 prescriptions including Angelicae Gigantis Radix and Cnidii Rhizoma in Dongeuibogam. Angelicae Gigantis Radix and Cnidii Rhizoma have been used separately or concurrently in Oriental Medicine for a long time as a treatment for various diseases. The following conclusions are reached through investigations on the applications of prescriptions including Angelicae Gigantis Radix and Cnidii Rhizoma in Dongeuibogam. 1. 34 times(15.9%) prescriptions are recorded in women chapter, 24 times(11.2%) in wind chapter, 13 times(6.1%) in children chapter and 12 times(5.6%) in intumescence chapter, which are arranged in order of frequency. 2. Treating blood, stroke and fetal movement are ranked top, regulating blood, headache, cataract, carbunculosis, pregnancy, abortion, underdevelopment, etc, among 137 symptoms in prescriptions including Angelicae Gigantis Radix and Cnidii Rhizoma. 3. The dosages of Angelicae Gigantis Radix and Cnidii Rhizoma are ranged from 2.5 pun~6 nyang. 1 jeon is recorded 54 times(25.2%), 7 pun is 29 times(13.6%), 5 pun is 24 times(11.2%), 1 nyang is 21 times(9.8%), same amount is 11 times(5.1%), 5 pun is 10 times(4.7%), 8 pun is 9 times(4.2%) used among 187 prescriptions including Angelicae Gigantis Radix and Cnidii Rhizoma. 4. The dosages of Angelicae Gigantis Radix and Cnidii Rhizoma are ranged from 2.5 pun~6 nyang. 2.5 pun~1.5 jeon, 2 jeon~6 nyang, same amount which are recorded by percent(decoction groups: the other groups) are 69.0%(98.0:2.0%), 25.5%(18.5:81.5%), 5.1%(18.2:81.8%) respectively. The less using dosage of Angelicae Gigantis Radix and Cnidii Rhizoma has a more percentage of decoctions groups, The more using dosage of Angelicae Gigantis Radix and Cnidii Rhizoma has a more percentage of the other groups except decoctions. The less using prescriptions have an effects of controling menstrual pain, enhancing blood and chi, etc, as a whole. The more using prescriptions as a form of compound powder have an effects in epilepsy, chronic diseases, etc. 5. The 214 prescriptions including Angelicae Gigantis Radix and Cnidii Rhizoma in Dongeuibogam are mainly composed of Goongguitang, Samooltang, Sagoonjatang and Gobangpoongtang, etc.

• 대한본초학회지
• /
• 제26권4호
• /
• pp.109-113
• /
• 2011

Objectives : We investigated the antioxidant and Antidiabetic effects of Cnidii Rhizoma Fermentata by Aspergillus oryzae and Aspergillus Kawauchi for 3days. Methods : In this study we compared Cnidii Rhizoma Fermentata with Aspergillus oryzae and Aspergillus Kawauchi that examined using reducing sugar, DPPH radical scavenging activity, ${\alpha}$-amylase and ${\alpha}$-glucosidase inhibitory activity. Also determined changes of pH and sugar content during fermentation for 3days. Results : The values for DPPH radical scavenging activity of Cnidii Rhizoma fermented by Aspergillus oryzae (86.6%) was higher than that of Aspergillus Kawauchi (77.9%). In ${\alpha}$-amylase inhibitory activity, fermented by Aspergillus Kawauchi had the highest inhibitory activity among other groups. But in ${\alpha}$-glucosidase inhibitory activity, fermented by Aspergillus oryzae had the highest inhibitory activity among other groups. While all groups of the sugar content increased During 3days fermentation, the pH was decreased. Conclusions : Based on these results, It was suggested that Cnidii Rhizoma Fermentata can be a useful and cost-effective resource for fuctional food and medicine.

• 대한약침학회지
• /
• 제12권2호
• /
• pp.31-40
• /
• 2009

Objective : This study was carried out to investigate protective effects of Pharmacopuncture Solutions (PSs) made by Carthmi Flos (CF), Cnidii Rhizoma (CR) and Astragali Radix (AR) on C6 glioma cells Methods : We investigated the effects of PSs on proliferation rates and types of C6 cells, and also investigated the effects on LDH release. In addition, protective effects of PSs on oxidative stress induced by hydrogen peroxide and SOD-like activities were also investigated. Results : PSs made by CF, CR and AR did not show cytotoxicity in various concentrations. CF-PS and AR-PS elevated levels of proliferation rates significantly. Treatment with CF-PS lowered level of LDH release in C6 cells. In addition, CF-PS and CR-PS showed protective effects on cell death induced by hydrogen peroxide respectively. Finally, CF-PS group showed high level of SOD-like activity compared to that in CR-PS group. Conclusion : These results suggest that CF-PS can accelerate proliferation of neuroglial cells, and has protective action against oxidative stress, which was involved in anti-oxidative effects such as SODlike activities. In addition, CR has protective effects against oxidative stress, and AR can accelerate proliferation of neuroglial cells.

• 대한본초학회지
• /
• 제20권4호
• /
• pp.95-101
• /
• 2005

Objectives : In order to distinguish Cnidii Rhizoma and Ligustici Chuanxiong Rhizoma (cultivated in Korea and China), their external and internal states were observed via microscopic examination. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : Cnidii Rhizoma, Ligustici Chuanxiong Rhizoma (from Korea), and Ligustici Chuanxiong Rhizoma (from China) all demonstrated similar patterns as following : 1. external form 1) showing irregular nodular form with $2{\sim}7cm$ diameter. 2) having several trochites in parallel, and nodulose root trace on surface. 3) in addition to yellow-white or yellow-gray intersections, yellow-brown oil sacs were distributed, with cambium resembling a form of annulus. 2. internal form 1) its root and rhizome is composed of 10 layers of cork, with narrow cortical layers. 2) its phloem is wide, and oval light yellow-brown oil sacs are distributed around. The closer to the cambium, the smaller it is. 3) cambium is a form of a wave-like ring or an irregular polygon. 4) xylem and vessel are rare, most of which are arranged in a 'V' format, and sometimes xylem fiber is found. 5) pith is composed of parenchymatous cell, and parenchyma is full of starch grains. Conclusions : In conclusion, C officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) have rhizomes in a similar form with no special difference. Therefore, it is concluded that C. officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) are categorized into the same species.

• 대한본초학회지
• /
• 제24권4호
• /
• pp.1-8
• /
• 2009

Objectives : This study was carried out to discriminate origin and molecular marker of oriental medicine "Cnidii Rhizoma" be circulated between Korea and China, which is difficult to discriminate from morphological distinction because of a fragmental materials of roots. Methods : Materials were collected randomly from a medicinal herb markets in Korea and China and be analyzed with ITS (internal transcribed spacer) regions of nuclear ribosomal DNA (nrDNA). Results : As a results, ITS regions of nrDNA was shown to be identify as three molecular markers. "Cnidii Rhizoma" was made up syster group of the genus Ligusticum L. and divided into three groups with "Tou-chun-gung", "IL-chun-gung" and "China-chun-gung". Conclusions : From the analysis of ITS regions of nrDNA, we presumed that it is the same origin of "Cnidii Rhizoma" from Korea and China because of phylogenetic tree consisted of sister groups with the genus Ligusticum than the genus Cnidium.

• 생약학회지
• /
• 제32권1호통권124호
• /
• pp.72-78
• /
• 2001

This study was performed to elucidate the influence on the plasma decursinol concentration after administration of combined decursin and Cnidii Rhizoma or decurisin and Bupleuri Radix extracts in comparison with single decursin administration. The identification of decursinol isolated from Angelica gigas was carried out by GC/MS and the concentration of decursinol in the plasma after oral administration was determined by HPLC. The value of area under the plasma concentration (AUC) and the maximal concentration (Cmax) of decursinol after administration of methanol and ether Angelicae Gigantis Radix extract was higher than those of single decursin-treated group. It was also found that the AUC and the Cmax of decursinol after combined administration with decursin and Cnidii Rhizoma were higher than those of decursin administration, whereas those of combined with decursin and Bupleuri Radix was lower. Moreover, the studies were performed with decursinol only or with combined decursinol and Cnidii Rhizoma/Bupleuri Radix, both of combined treatment increased the plasma decursinol concentrations compared with decursinol-treated group in rats. On the basis of these results, it is concluded that co-existing substances or co-administration with other drugs may influence the plasma levels of decurisinol after oral administration.