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Development and Validation of Quick and Accurate Cephalopods Grouping System in Fishery Products by Real-time Quantitative PCR Based on Mitochondrial DNA (두족류의 진위 판별을 위한 Real-time Quantitative PCR 검사법 개발 및 검증)

  • Chung, In Young;Seo, Yong Bae;Yang, Ji Young;Kwon, Ki sung;Kim, Gun Do
    • Journal of Food Hygiene and Safety
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    • v.33 no.4
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    • pp.280-288
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    • 2018
  • In this study, an approach for the analysis of the five cephalopod species (octopus, long-arm octopus, squid, wet-foot octopus, beka squid) consumed in the Republic of Korea is developed. The samples were collected from the Southeast Asian countries Thailand, Indonesia, Vietnam, and China. The SYBR-green-based real-time qPCR method, based on the mitochondrial DNA genome of the five cephalopods was developed and validated. The intergroup variations in the mitochondrial DNA are evident in the bioinformatic analysis of the mitochondrial genomic DNA sequences of the five groups. Some of the highly-conserved and slightly-variated regions are identified in the mitochondrial cytochrome-c-oxidase subunit I (COI) gene, 16s ribosomal RNA (16s rRNA) gene, and 12s ribosomal RNA (12s rRNA) gene of these groups. To specify each five cephalopod groups, specific primer sets were designed from the COI, 16s rRNA and 12s rRNA regions. The specific primer sets amplified the DNA using the SYBR-green-based real-time PCR system and 11 commercially secured animal tissues: Octopus vulgaris, Octopus minor, Todarodes pacificus, Dosidicus gigas, Sepia esculenta, Amphioctopus fangsiao, Amphioctopus aegina, Amphioctopus marginatus, Loliolus beka, Loligo edulis, and Loligo chinensis. The results confirmed by a conveient way to calculate relative amplification levels between different samples in that it directly uses the threshold cycles (Ct)-value range generated by the qPCR system from these samples. This genomic DNA-based molecular technique provides a quick, accurate, and reliable method for the taxonomic classification of the animal tissues using the real-time qPCR.

Edge Property of 2n-square Meshes as a Base Graphs of Pyramid Interconnection Networks (피라미드 상호연결망의 기반 그래프로서의 2n-정방형 메쉬 그래프의 간선 특성)

  • Chang, Jung-Hwan
    • The Journal of the Korea Contents Association
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    • v.9 no.12
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    • pp.582-591
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    • 2009
  • The pyramid graph is an interconnection network topology based on regular square mesh and tree structures. In this paper, we adopt a strategy of classification into two disjoint groups of edges in regular square mesh as a base sub-graph constituting of each layer in the pyramid graph. Edge set in the mesh can be divided into two disjoint sub-sets called as NPC(represents candidate edge for neighbor-parent) and SPC(represents candidate edge for shared-parent) whether the parents vertices adjacent to two end vertices of the corresponding edge have a relation of neighbor or shared in the upper layer of pyramid graph. In addition, we also introduce a notion of shrink graph to focus only on the NPC-edges by hiding SPC-edges in the original graph within the shrunk super-vertex on the resulting graph. In this paper, we analyze that the lower and upper bound on the number of NPC-edges in a Hamiltonian cycle constructed on $2^n\times2^n$ mesh is $2^{2n-2}$ and $3*(2^{2n-2}-2^{n-1})$ respectively. By expanding this result into the pyramid graph, we also prove that the maximum number of NPC-edges containable in a Hamiltonian cycle is $4^{n-1}-3*2^{n-1}$-2n+7 in the n-dimensional pyramid.

A State-of-the Art Review of Clothing and Textiles Research in Korea : 1991~1999 (한국의류학의 연구경향분석 : 1991~1999)

  • 나수임;이정순;배주형
    • The Research Journal of the Costume Culture
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    • v.8 no.6
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    • pp.853-863
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    • 2000
  • This study is planned to present the future direction of the study of Korean clothes through the realistic recognition of the changes of studying issues of each area of the related clothes. For this purpose, the writer of this thesis have collected, classified and analyzed those various kinds of papers and theses published in the professional magazines of clothes such as Journal of Korean Home Economics, Journal of the Korean Society of Clothing and Textiles, Journal of the Korean Society of Costume, The Research Journal of the Costume Culture so that I might examine and study the main trend of the related study. The result of the study is as follows. 1. Since the first publication of Journal of Korean Home Economics in 1959 and Journal of the Korea Society of Clothing and Textiles, and Journal of the Korean Society of Costume, the study of clothes has been gradually increased. Furthermore, since 1990 the issuing times of various kinds of Journals have been continuously increased with the foundation of The Research Journal of the Costume Culture and Journal of Fashion Business. 2. Theses and papers published in the four greatest Journals of Korean Clothing and Textiles have been studies for the detailed classification and analysis of the data. According to the study, It is found that the theses in Journal of the Korea Society of Clothing and Textiles have actively studied textiles as they had studied before 1990s and those in Journal of the Korean Society of Costume have studied history of costumes and those in The Research Journal of the Costume Culture have studied the composition and science of clothes. 3. The order of studying subjects was 1) Design and Aesthetics, 2) Fashion Merchandising 3) Textiles, 4) Clothing Construction, 5) History of Costumes, 6) Costume Culture, 7) Socio-Psychology of clothing, 8) Others. Considering the above mentioned result of the study, most of the main fields of the clothing and textiles study have been studied continuously in balance since 1990 unlike the past although the past studies had been performed largely in some main fields only. And it was found that fields of clothing styles and clothing goods have been studied most actively since 1990s. 4. The studying field of Design and Aesthetics has showed to have the trend to be studied very actively since 1990s, which has utilized various relevant data with scholastic approach to psychology and aesthetics. Regarding Fashion Merchandising, the study of consumers decision process has been performed most actively. And theses on the international trades have been published considerably more than ever before, which resulted in gradual increase in gradual increase in the study of the fields related with global marketing, internet marketing, textile products exports, and overseas brands, etc. Regarding Textiles, the field of clothing sanitation and management has been studied more actively than that of clothing materials. In case of clothing construction, the study of physical styles and pattern making has been more active than the other related fields. Fields of socio-psychology of clothing and history costume have been a little reduced. Field of costume culture has been firmly rooted as an independent subject to be studied in the clothing related study. And study of the clothes has shown various trends of studies of ethnic & folk clothes, religious and ceremonial garments, linguistic & favorite approach to clothes and academic analysis of the data.

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Analysis of Pretreatment Prognostic Factors in Locally Advanced Carcinoma of the Uterine Cervix (국소진행된 자궁경부암에 있어서의 예후인자 분석)

  • Oh, Do-Hoon;Ha, Sung-Whan;Lee, Moo-Song
    • Radiation Oncology Journal
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    • v.10 no.1
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    • pp.69-76
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    • 1992
  • To identify pretreatment prognostic factors in locally advanced carcinoma of the uterine cervix, retrospective analysis was undertaken of 154 patients treated with curative radiation therapy at Seoul National University Hospital, from March 1979 through December 1986. According to FIGO classification, eight patients were stage IIIA, 134 were stage IIIB, and 12 were stage IVA. Five year locoregional control rate was $58\%$, $51\%$, and $27\%$ in stage IIIA, IIIB, and IVA, respectively. Five year disease free survival was $57\%$, $40\%$, and $25\%$ for each stage respectively. Five year overall survival was $67\%$, $51\%$, and $33\%$ in stage IIIA, IIIB, and IVA, respectively. In univariate analysis, fewer than or equal to four of pregnancies, initial hemoglobin of lower than $10\;g\%$, and pelvic sidewall invasion on CT were associated with poor locoregional control. Number of pregnancies, initial hemoglobin level, obstructive uropathy on intavenous pyelography (IVP), pelvic lymph node (LN) status on CT, and pelvic sidewall invasion on CT were significant factors in disease free survival. In terms of overall survival, pelvic sidewall invasion on CT and bladder invasion on CT were prognostically significant. In multivariate analysis, no factor was found to affect locoregional control and pelvic LN status was a sole significant factor affecting disease free survival. in terms of overall survival, the size.

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A Comparison Study of the Amplification Characteristics of the Seismic Observation Sites using Coda wave, Background Noise, and S-wave Energy from Fukuoka Earthquakes Series (후쿠오카 지역 발생 지진의 Coda파, 배경잡음 및 S파 에너지를 이용한 관측소의 증폭특성에 관한 비교 연구)

  • Kim, Jun Kyoung
    • The Journal of Engineering Geology
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    • v.23 no.4
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    • pp.435-445
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    • 2013
  • Since design response spectrum does not reflect local soil characteristics, site specific response spectrum of observed ground motions appears relatively higher than design response spectrum at high frequency range. These problems have been pointed out from the domestic seismic design industry. Among various estimation methods, this study used the method H/V ratio of ground motion for estimating site amplification. This method has been extended to background noise, Coda waves and S waves recently for estimating site amplification. This study applied this method to the background noise and Coda wave energy. This study analysed more than 267 background noises from 15 macro earthquakes including main Fukuoka earthquake (2005/03/20, M=6.5) and then compared to results from S waves, at 8 main domestic seismic stations. The results showed that most of the domestic seismic stations gave similar results to those from S waves. Each station showed its own characteristics of site amplification property in low, high and specific resonance frequency ranges. Comparison of this study to other studies using different method can give us much information about dynamic amplification of domestic sites characteristics and site classification.

The Relationship between Perceived Stress Scale and Carotid Intima-Media Thickness Using Ultrasonography in University Students (대학생들의 스트레스 지각 정도와 경동맥 내막-중막 두께의 관계 연구)

  • Kim, Dae-Sik;Sung, Hyun-Ho;Lee, Jong-Woo;Cho, Eun-Kyung
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.3
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    • pp.308-315
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    • 2017
  • Carotid artery intima-media thickness (CIMT) test is a standardized ultrasound procedure that screens for and monitors atherosclerosis in a safe and non-invasive manner, even in individuals with no symptoms of heart disease. The purpose of this study was to research the relationship between CIMT, as measured by ultrasonography, and the degree of individual perceived stress. There was a total of 168 (male: 46, female: 122) university students in Suwon-si, Gyeonggi-do, Korea, without other diseases. Written informed consent was obtained from all participants. CIMT was measured in the right and left common carotid arteries 1 cm below the bifurcation, and internal carotid artery, using non-invasive high-resolution Medison Accuvix V20 prestige ultrasound equipment and perceived stress, was measured with the 10-item PSS (Perceived Stress Scale) questionnaire. This study indicates that PSS may be appropriate to estimate perceived stress levels and weight. Failure to control stress anxiety could lead to weight and CIMT soaring to dangerous levels, resulting in a myocardial infarction or cerebrovascular accident. There was a statistically significant difference between left CIMT (p<0.05) and weight (p<0.05) according to each stress classification. The results of this study suggest that the weight measurement is influenced in part by one's generalized stress levels. Future continuous studies should be conducted to test the influences of perceived stress and generalized anxiety on CIMT when these are many continuous variables.

Intake of antioxidants and B vitamins is inversely associated with ischemic stroke and cerebral atherosclerosis

  • Choe, Hansaem;Hwang, Ji-Yun;Yun, Jin A;Kim, Ji-Myung;Song, Tae-Jin;Chang, Namsoo;Kim, Yong-Jae;Kim, Yuri
    • Nutrition Research and Practice
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    • v.10 no.5
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    • pp.516-523
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    • 2016
  • BACKGROUND/OBJECTIVES: This study was conducted to examine relationships between dietary habits and intakes of antioxidants and B vitamins and the risk of ischemic stroke, and to compare dietary factors according to the presence of cerebral artery atherosclerosis and stroke subtypes. SUBJECTS/METHODS: A total of 147 patients and 144 control subjects were recruited consecutively in the metropolitan area of Seoul, Korea. Sixty participants each in the case and control groups were included in analyses after 1:1 frequency matching. In addition, 117 acute ischemic stroke patients were classified into subtypes according to the Trial of Org 10172 in Acute Stroke Treatment (TOAST) guidelines. Dietary intake was measured using a semi-quantitative food frequency questionnaire composed of 111 food items and plasma lipid and homocysteine levels were analyzed. RESULTS: When compared with control subjects, stroke patients had unfavorable dietary behaviors and lower intakes of fruits ($73.1{\pm}83.2g$ vs. $230.9{\pm}202.1g$, P < 0.001), vegetables ($221.1{\pm}209.0g$ vs. $561.7{\pm}306.6g$, P < 0.001), and antioxidants, including vitamins C, E, $B_6$, ${\beta}$-carotene, and folate. The intakes of fruits, vegetables, vitamin C, and folate were inversely associated with the risk of ischemic stroke after adjusting for confounding factors. Intakes of vegetables, vitamins C, $B_6$, $B_{12}$, and folate per 1,000 kcal were lower in ischemic stroke with cerebral atherosclerosis than in those without. Overall vitamin $B_{12}$ intake per 1,000 kcal differed according to the TOAST classification (P = 0.004), but no differences among groups existed based on the post-hoc test. CONCLUSIONS: When compared with control subjects, ischemic stroke patients, particularly those with cerebral atherosclerosis, had unfavorable dietary intake, which may have contributed to the development of ischemic stroke. These results indicate that proper dietary recommendations are important for the prevention of ischemic stroke.

Comparison of Artificial Neural Network and Empirical Models to Determine Daily Reference Evapotranspiration (기준 일증발산량 산정을 위한 인공신경망 모델과 경험모델의 적용 및 비교)

  • Choi, Yonghun;Kim, Minyoung;O'Shaughnessy, Susan;Jeon, Jonggil;Kim, Youngjin;Song, Weon Jung
    • Journal of The Korean Society of Agricultural Engineers
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    • v.60 no.6
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    • pp.43-54
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    • 2018
  • The accurate estimation of reference crop evapotranspiration ($ET_o$) is essential in irrigation water management to assess the time-dependent status of crop water use and irrigation scheduling. The importance of $ET_o$ has resulted in many direct and indirect methods to approximate its value and include pan evaporation, meteorological-based estimations, lysimetry, soil moisture depletion, and soil water balance equations. Artificial neural networks (ANNs) have been intensively implemented for process-based hydrologic modeling due to their superior performance using nonlinear modeling, pattern recognition, and classification. This study adapted two well-known ANN algorithms, Backpropagation neural network (BPNN) and Generalized regression neural network (GRNN), to evaluate their capability to accurately predict $ET_o$ using daily meteorological data. All data were obtained from two automated weather stations (Chupungryeong and Jangsu) located in the Yeongdong-gun (2002-2017) and Jangsu-gun (1988-2017), respectively. Daily $ET_o$ was calculated using the Penman-Monteith equation as the benchmark method. These calculated values of $ET_o$ and corresponding meteorological data were separated into training, validation and test datasets. The performance of each ANN algorithm was evaluated against $ET_o$ calculated from the benchmark method and multiple linear regression (MLR) model. The overall results showed that the BPNN algorithm performed best followed by the MLR and GRNN in a statistical sense and this could contribute to provide valuable information to farmers, water managers and policy makers for effective agricultural water governance.

A Study on Evaluation of the Rurality by the Distance of View (조망거리에 따른 농촌경관의 농촌다움 평가에 관한 연구)

  • Shin, Min-Ji;Shin, Ji-Hoon
    • Journal of Korean Society of Rural Planning
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    • v.21 no.2
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    • pp.63-77
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    • 2015
  • Improve the rural views of the Government and the municipalities with the intention of rural landscape planning. However, rural scenery evaluation of landscape types and element categories, landscape scenes, such as the valuation of resources selected, form a coherent system and ecological point of view is not only a Visual perspective of evaluation, or evaluation is made. This study evaluates the rural landscape through the rural landscape for rurality is implemented can explain the image. Rural amenity 100 represented the rural landscape in rurality with photos of photos, and scenes, photos, location-based rural Vista rurality landscapes drawn components. Selected landscape photography scenic landscapes in the u.s. survey and image vocabularies through surveys is representative of the landscape in rurality of each Vista location-specific photos and images began extracted factors. As a result, the Distant view is a town and village in the rolling hills of the surrounding environment is an image behind the Vista Skyline, Farmland Rural village forests, Behind the hills, Individual housing roof, Housing arrangement, The number of household includes landscape components. Factor analysis 'openness', 'intimacy', 'activity', 'complexity', 'safety' was down to five factors, such as a description of the overall factors that force was 66.45 percent. Middle distance view is close to being out of town houses and village environment, an image in the skyline hills Vista, farmlands, village forests, rolling hills, behind the scenes of the housing component of the neck, the sperm will honor an individual factor analysis results are 'intimacy', 'safety', 'openness', 'specificity', 'complexity' five factors the whole description of the capacity factors compared to 67.24 percent. Close-range view is mainly in Vista village embraced the individual elements and an image of a harmonious location in the surroundings of the sperm, individual houses, fences, gates, courtyard, Garden, garage, agricultural facilities, including, but not limited to, factors assay but an 'intimacy', 'complexity', 'safety', 'activity', 'openness' five factors, such as a description of the overall factors that force was 65.29%. This research seeks to determine the extent to which rurality of rural landscape Vista by location attributes and elements and representative officer in photo selection and rurality to extract the image. To date, followed by a comprehensive study, which was presented through the readjustment could not overlook the Visual elements. The future rural development officer for the evaluation of rural landscape classification and quantitative and objective assessment will have to come up with ways to do.

A Software Error Examination of 3D Automatic Face Recognition Apparatus(3D-AFRA) : Measurement of Facial Figure Data (3차원 안면자동인식기(3D-AFRA)의 Software 정밀도 검사 : 형상측정프로그램 오차분석)

  • Seok, Jae-Hwa;Song, Jung-Hoon;Kim, Hyun-Jin;Yoo, Jung-Hee;Kwak, Chang-Kyu;Lee, Jun-Hee;Kho, Byung-Hee;Kim, Jong-Won;Lee, Eui-Ju
    • Journal of Sasang Constitutional Medicine
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    • v.19 no.3
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    • pp.51-61
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    • 2007
  • 1. Objectives The Face is an important standard for the classification of Sasang Constitutions. We are developing 3D Automatic Face Recognition Apparatus(3D-AFRA) to analyse the facial characteristics. This apparatus show us 3D image and data of man's face and measure facial figure data. So We should examine the Measurement of Facial Figure data error of 3D Automatic Face Recognition Apparatus(3D-AFRA) in Software Error Analysis. 2. Methods We scanned face status by using 3D Automatic Face Recognition Apparatus(3D-AFRA). And we measured lengths Between Facial Definition Parameters of facial figure data by Facial Measurement program. 2.1 Repeatability test We measured lengths Between Facial Definition Parameters of facial figure data restored by 3D-AFRA by Facial Measurement program 10 times. Then we compared 10 results each other for repeatability test. 2.2 Measurement error test We measured lengths Between Facial Definition Parameters of facial figure data by two different measurement program that are Facial Measurement program and Rapidform2006. At measuring lengths Between Facial Definition Parameters, we uses two measurement way. The one is straight line measurement, the other is curved line measurement. Then we compared results measured by Facial Measurement program with results measured by Rapidform2006. 3. Results and Conclusions In repeatability test, standard deviation of results is 0.084-0.450mm. And in straight line measurement error test, the average error 0.0582mm, and the maximum error was 0.28mm. In curved line measurement error test, the average error 0.413mm, and the maximum error was 1.53mm. In conclusion, we assessed that the accuracy and repeatability of Facial Measurement program is considerably good. From now on we complement accuracy of 3D-AFRA in Hardware and Software.

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