• Korean Journal of Weed Science
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• v.10 no.3
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• pp.214-220
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• 1990

To establish a greening assay for screening, and physiological and biochemical studies of the compounds affecting biosynthesis of plant pigments, were conducted on environmental factors, and on ways of incubation and illumination which affect plant greening. Greening was good when both cucumber and barley were grown for 5 to 6 days at $25^{\circ}C$ in darkness, when adaxial sides of cucumber cotyledons were contacted with the solution, and when barley leaf fragments were taken 0.5 to 2.0cm from the leaf tip. Potassium phosphate buffer(pH 6.0) at 10mM was most desirable for plant greening. The speed of greening during illumination was increased as the temperature increased from $15^{\circ}C$ to $35^{\circ}C$. The responses were sensitive more in cucumber than in barley, and in chlorophyll biosynthesis than in carotenoid biosythesis. The content of chlorophyll was greatest at the light intensity of 5000 and 1000 lux for cucumber and barley, respectively, but the biosynthesis of carotenoids were greatest at the light intensity higher than for chlorophyll. In use of solvents for dissolving chemicals, acetone, ethyl alcohol and DMSO at 10, 0.1 and 2.5% or less, respectively, did not affect the biosynthesis of plant pigments. $pI_{50}$ values were calculated for chemicals affecting pigment biosynthesis.

• Environmental Analysis Health and Toxicology
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• v.15 no.1_2
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• pp.13-18
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• 2000

A few in vitro methods were developed to compare the result on the phototoxicity of phenothiazines. By the MTT assay, the Candida test, and the RBC photohemolysis, the phototoxicities of UVA and UVB irradiation were measured. This paper presents the comparisons of methods which are effective to measure the phototoxicities of the chemicals causing phototoxicity and photoallergy. The tested chemicals of phenothiazines include Chlorpromazine, Promethazine, Perphenazine, Chlorprothixene, Trifluoperazine and Thioridazine. Each chemical represented variable results according to the test methods. MTT assay shows the most sensitive method.

• Archives of Pharmacal Research
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• v.27 no.2
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• pp.173-176
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• 2004

Three phenolic compounds, rosmarinic acid (1), methyl rosmarinate (2), ethyl rosmarinate (3), and two flavonoids, luteolin (4), luteolin-7-O-$\beta$-D-glucuronide methyl ester (5) were isolated from the aerial part of Lycopus lucidus (Labiatae). Their structures were determined by chemical and spectral analysis. Compounds 1-5 exhibited potent antioxidative activity on the NBT superoxide scavenging assay. The $IC_{50}$ values for compounds 1-5 were 2.59, 1.42, 0.78, 2.83, and 3.05 $\mu\textrm{g}$/mL respectively. In addition, five compounds were isolated from this plant for the first time.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.328.2-328.2
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• 2002

This paper presents stability of tyhoid vaccine -attenuuated vaccine(oral) and killed vaccine{vi polysaccharide)- 5 classes with various temperature(3 points: iced temperature, refrigeration temp.. indoor temp.). Analytical techniques -vi polysaccharide content. pH. sterility. assay - have been used for the quantity of pharmacologically active chemical entries. From this study. we have found the attenuated vaccine is show iced temp. 258% and indoor temp. 0.02% compare with refrigeration temp. in assay examination and the killed vaccine is not found different. (omitted)

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.1
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• pp.27-34
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• 2015

Many applications of nanoparticles have been developed since 1970s. Surface plasmon resonance (SPR) effect can be generated at the surface of nanoparticles by illumination. SPR is the resonant oscillation of conduction electrons at the surface material stimulated by incident light. The collisions between excited electrons and metal atoms can cause the production of thermal energy (photothermal effect). Here, we presented the development of thermo-cosmetics using photothermal effect of gold nanoparticles. Gold nanoparticles (GNPs) were chosen for it's low toxicity. We also and investigated the cell biocompatibility and heating effectiveness for photothermal effect of GNPs. Synthesized GNPs were verified by UV-vis spectrophotometer, where GNP has a characteristic absorbance spectrum. Concentration of GNP was measured by atomic absorption analyzer. The cytotoxicity was confirmed by MTT assay and double staining assay. Photothermal effect of GNP was demonstrated by the thermal increasing properties depending on GNP concentration, which was taken by an IR-thermal camera with a xenon lamp as the light source. If the thermal effect of GNP is applied for thermo-cosmetics, it can supply heat to skin by converting solar energy into thermal energy. Thus, cosmetics containing GNPs can provide benefits to people in the cold region or winter season for maintaining skin temperature, which lead to a positive effect on skin health.

• Bulletin of the Korean Chemical Society
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• v.33 no.5
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• pp.1693-1698
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• 2012

A headspace gas chromatographic mass spectrometric (GC-MS) assay method was developed for the simultaneous determination of methyl tertiary butyl ether (MTBE), $tert$-butyl alcohol (TBA) and benzene, toluene, ethyl benzene and xylene (BTEX) in soil contaminated with gasoline. 2 g of soil sample were placed in a 10 mL headspace vial filled with 5 mL of phosphoric acid solution (pH 3) saturated with NaCl, and the solution was spiked with fluorobenzene as an internal standard and sealed with a cap. The vial was heated in a heating block for 40 min at $80^{\circ}C$. The detection limits of the assay were 0.08-0.12 ${\mu}g$/kg for the analytes. For five independent determinations at 10 and 50 ${\mu}g$/kg, the relative standard deviations were less than 10%. The method was used to analyze fifty six soil samples collected from various regions contaminated with gasoline in Korea. The developed method may be valuable for the monitoring of the analytes in soil.

• Bulletin of the Korean Chemical Society
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• v.27 no.10
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• pp.1613-1617
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• 2006

Optimum analytical conditions for cyclic voltammetry (CV) and square wave (SW) stripping voltammetry were determined using mercury-mixed carbon nanotube paste electrode (PE). The results approached the microgram working ranges of SW: 10.0-80.0 $ugL^{-1}$ and CV: 100-700 $ugL^{-1}$ Cd (II); working conditions of 300-Hz frequency, 100 mV amplitude, 1.6 V accumulation potential, 400 sec accumulation time, and 40 mV increment potential. First, analysis was performed through direct assay of cadmium ions deep into the fishs brain core and plant tissue in real time with a preconcentration time of 400 sec. The relative standard deviation of 10.0 $mgL^{-1}$ Cd (II) observed was 0.064 (n = 12) at optimum conditions. The low detection limit (S/N) was set at 0.6 $ugL^{-1}$ ($5.33{\times}10^{-9}$ M). The methods can be used in direct analysis in vivo or in real-time monitoring of plant tissue.

• Biomedical Science Letters
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• v.15 no.2
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• pp.105-112
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• 2009

The screening of the anti-cancer activity of the chemical library provided 2-thio-4-aminopyrimidine as the initial hit. The confirmation of structure and biological effect of hit was performed by synthesis and biological evaluation. The optimization of hit was performed by derivatization of substituents while keeping the core structure. The evaluation of growth inhibitory activity was carried out using SRB assay against 6 human cancer cell lines and human fibroblast. The growth inhibitory activity of compounds showed substituent dependency and more than 5 compounds showed complete growth inhibition of cancer cell lines at 10 ${\mu}M$ concentration. Chemical library screening followed by synthetic modification provided possibility that 2-thio-4-aminopyrimidine can be used as a new scaffold for the development of anti-cancer agent.

• KSBB Journal
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• v.27 no.2
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• pp.131-135
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• 2012

To develop potential cosmetic ingredients with antimicrobial and antioxidant activities of 4 Korean bamboo species (P. bambusoides, P. nigra var. henonis, P. pubescens and Sasa coreana) using three different extraction methods-water, ethanol and supercritical fluid extraction. Antimicrobial activities and DPPH assay have been examined. Among the antimicrobial activities against two test strains, Escherichia coli and Staphylococcus aureus, ethanol extracts of 3 bamboo trees, P. bambusoides, P. nigra var. henonis, and P. pubescens, showed stronger than those of supercritical extracts. However, 4 bamboo supercritical extracts showed dose-dependent increase in antioxidant activity by DPPH assay. These results suggest that water fraction of bamboo extracts may be useful for the cosmetic ingredient with low cytotoxicity.

• The Plant Pathology Journal
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• v.34 no.2
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• pp.85-92
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• 2018

Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QD-based biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.