• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.29 no.1
• /
• pp.16-32
• /
• 2016

Objective : The water extract of Bojungikgi-tang (BTE) composed with Ginseng Radix, Astragali Radix, Angelicae gigantis Radix, Atractylodes Rhizoma alba, Aurantii nobilis Pericarpium, Glycyrrhizae Radix, Cimicifuga Rhizoma, and Bupleuri Radix and it has been traditionally used for chronic diseases or weakness after illness in oriental countries. However, little is known about the effects of aqueous extract of BTE on trimellitic anhydride (TMA)-induced contact hypersensitivity (CHS). Therefore, the aim of this study was to investigate the effects of BTE on TMA-induced CHS in BALB/c mice.Methods : In this study, the extract of BTE was prepared by extracting with distilled water at 100℃ for 2.5 h. The extract was freeze-dried following filtration through 0.45 μm filter. Mice were orally administrated with or without BTE extract of different doses (25-200 ㎎/㎏/day) for 28 days. During the challenge period, mice were externally applied with different doses of BTE extract one time per day 30 min before TMA treatment. I examined the effects of BTE on the serum levels of IgE, IgG1, IgG2a, and IgG2b, nitric oxide (NO) and prostaglandin E₂ (PGE₂) production in isolated peritoneal macrophages, Th2 cytokine production in isolated spleen cells, ear swelling responses, and the leukocyte infiltration induced by TMA.Results : The orally and externally administration of BTE dose-dependently reduced the serum levels of hapten-specific immunoglobulin E (IgE), IgG1 titer, and PGE₂ production as well as ear swelling responses and leukocyte infiltration in TMA-induced BALB/c mice. The levels of NO and PGE₂ production from peritoneal macrophages stimulated with TMA-BSA were markedly suppressed by pretreatment with BTE in a concentration dependent manner. Furthermore, the levels of Th2 cytokines (IL-4, IL-5, IL-13) production from spleen cells stimulated with ConA were markedly suppressed by BTE treatment in a concentration dependent manner.Conclusions : These results suggest that BTE treatment suppresses chronic contact hypersensitivity, and it can be assumed that the suppression of ear swelling, serum IgE, NO and PGE₂ levels, leukocyte infiltration, and Th2 cytokines in an animal model. Therefore, these properties may contribute to the strong anti-CHS response effect of BTE.

• International Commerce and Information Review
• /
• v.15 no.1
• /
• pp.227-256
• /
• 2013

Existing multinational subsidiary typologies seem to have limitations in two respects. First, the prevalence of subsidiary classification along two-dimensions fails to capture many distinct subsidiary types. Failure to reflect a sufficient richness in dimensionality can give rise to a partial picture of subsidiary typologies in the international business literature. A new typology developed from multi-dimensional approach will be required for reflecting various subsidiary roles in the multinational enterprise. Second, multinational subsidiary performing a number of activities is hard to be defined functionally across the value chain activities. In addition, multinational subsidiary roles can vary dramatically. In conclusion, despite a growing amount of work on subsidiary typologies, there seems to be limited convergence of results. the study regarding subsidiary roles still remain a challenge. In this respect, the purpose of this study is to develop a new typology based on multi-dimensional approach in order to overcome the limitations of traditional typologies. To classify subsidiary types, we propose 8 types of multinational subsidiary according to three dimensions that are adopted: (1) number of required value chain activities (2) subsidiary's sourcing capability and autonomy (3) global orientation (3) The case study analyzing Korean foreign subsidiaries appropriate for 8 types is performed to establish the validity of this study.

• Journal of Ginseng Research
• /
• v.44 no.2
• /
• pp.247-257
• /
• 2020

Background: Multidrug resistance (MDR) to chemotherapy drugs remains a major challenge in clinical cancer treatment. Here we investigated whether and how ginsenoside Rg5 overcomes the MDR mediated by ABCB1 transporter in vitro and in vivo. Methods: Cytotoxicity and colon formation as well as the intracellular accumulation of ABCB1 substrates were carried out in MDR cancer cells A2780/T and A549/T for evaluating the reversal effects of Rg5. The expressions of ABCB1 and Nrf2/AKT pathway were determined by Western blotting. An A549/T cell xenograft model was established to investigate the MDR reversal activity of Rg5 in vivo. Results: Rg5 significantly reversed ABCB1-mediated MDR by increasing the intracellular accumulation of ABCB1 substrates without altering protein expression of ABCB1. Moreover, Rg5 activated ABCB1 ATPase and reduced verapamil-stimulated ATPase activity, suggesting a high affinity of Rg5 to ABCB1 binding site which was further demonstrated by molecular docking analysis. In addition, co-treatment of Rg5 and docetaxel (TXT) suppressed the expression of Nrf2 and phosphorylation of AKT, indicating that sensitizing effect of Rg5 associated with AKT/Nrf2 pathway. In nude mice bearing A549/T tumor, Rg5 and TXT treatment significantly suppressed the growth of drug-resistant tumors without increase in toxicity when compared to TXT given alone at same dose. Conclusion: Therefore, combination therapy of Rg5 and chemotherapy drugs is a strategy for the adjuvant chemotherapy, which encourages further pharmacokinetic and clinical studies.

• The Journal of Information Technology
• /
• v.1 no.1
• /
• pp.85-101
• /
• 1998

Information system, organization structure, and business strategy have been major variables in MIS research. Most of research about these variables interested in the relationship of two variables. There were very few efforts to connect three variables. But relationship or fitness of these factors plays an important role to improve performance. Nowadays managers face new challenge. That is 'globalization' To cope with this turbulent environment, they have to know how to fit these Important factors(information system, organization structure, business strategy). It is the main purpose of this article to find out the fitness of these variables under global business environment. Based on literature review, I made research model which tells whether fitness of three variables affects performance under the control of headquarter. And I propose hypothesis. I define strategy as global innovator, integrated player, implementers role, local innovator and organization structure as multinational, global, international, trans-national and information system as independent IT operation, headquarter driven configuration, intellectual synergy configuration, global integrated IT configuration.

• Korean Journal of Veterinary Service
• /
• v.43 no.2
• /
• pp.89-97
• /
• 2020

This study was carried out to examine a novel inactivated Salmonella Typhimurium (S. Typhimurium) vaccine candidate for protection of mice against salmonellosis by immunization of BALB/c mice using various type adjuvant. The novel type-inactivated vaccine candidate was constructed by adding Chlorhexidine digluconate solution. BALB/c mice were divided into 6 groups of 15 mice apiece. The mice were intramuscularly (IM) primed at 6 weeks of age and were IM boosted 8 weeks of age. Groups A and B mice were injected with sterile phosphate-buffered saline as controls; group C mice were inoculated with 5×10⁸ cells/100 µL of formalin-inactivated S. Typhimurium cells and adjuvant ISA70; groups D~F mice were immunized with 5×10⁸ cells/100 µL of the inactivated vaccine candidate and adjuvant ISA70, adjuvant IMS1313 and adjuvant IMS1313 containing 30 ㎍/mL of GI24, respectively. All mice (except group A mice) were orally challenged with a virulent S. Typhimurium strain at 10 weeks of age. Mice from groups C-F had significantly increased IgG levels compared to control groups (A-B) mice. The levels of splenocyte IFN-γ and IL-4 in mice of all groups were measured by ELISA, resulting in increased immunity in group F mice compared to those of groups A-E mice. These data suggested that systemic and cell-mediated immune responses were highly induced by IM immunization with the vaccine candidate and adjuvant IMS1313 containing GI24. Furthermore, clinical signs such as death were observed in only 20% of group F mice after virulent Salmonella strain challenge, however, groups B and C (100%), and groups D and E (60%) mice died. This data suggested that mice immunized by intramuscular prime and booster with this vaccine candidate and adjuvant IMS1313 containing GI24 effectively protected mice from salmonellosis.

• Journal of Legislation Research
• /
• no.53
• /
• pp.213-267
• /
• 2017

Regulatory barrier is considered most challenging out of all FinTech barriers, which many technology innovators have always experienced. Even though technological solutions promise customers accessibility to more cost-effective and secured financial services, it is quite challenging to create regulatory environment that enables innovation FinTech industry. Especially, a common challenge FinTech innovators and business face is regulatory uncertainty and confusion rather than any particular regulation. Since many FinTech models are continuously introducing new innovative ways in providing financial services, significant confusion could be raised in applying principles of existing law and regulations. In addition, it is uncertain whether or not applying complex regulatory compliance model intended for large financial institutions to small start-ups is appropriate since most existing regulations and rules are established and introduced without considering innovative tools such as mobile instruments, e-trade, and internet. Therefore, new mechanism to access to regulatory information in a more cost-effective, quick and immediate way should be created. Regulators, technological innovators, and financial customers should cooperate each other to find out appropriate solutions for those issues. Many regulators are introducing regulatory sandbox which provides service providers with opportunities to test their innovations, during the test, providing regulators with enough time to understand risks of innovations. However, regulatory sandbox is not a panacea for all challenges to FinTech innovations. Therefore, regulators should make comprehensive and multidimensional efforts including regulatory sandbox in supporting FinTech ecosystem.

• Biomolecules & Therapeutics
• /
• v.27 no.3
• /
• pp.327-335
• /
• 2019

As the elderly population is increasing, Alzheimer's disease (AD) has become a global issue and many clinical trials have been conducted to evaluate treatments for AD. As these clinical trials have been conducted and have failed, the development of new theraphies for AD with fewer adverse effects remains a challenge. In this study, we examined the effects of Theracurmin on cognitive decline using 5XFAD mice, an AD mouse model. Theracurmin is more bioavailable form of curcumin, generated with submicron colloidal dispersion. Mice were treated with Theracurmin (100, 300 and 1,000 mg/kg) for 12 weeks and were subjected to the novel object recognition test and the Barnes maze test. Theracurmin-treated mice showed significant amelioration in recognition and spatial memories compared those of the vehicle-treated controls. In addition, the antioxidant activities of Theracurmin were investigated by measuring the superoxide dismutase (SOD) activity, malondialdehyde (MDA) and glutathione (GSH) levels. The increased MDA level and decreased SOD and GSH levels in the vehicle-treated 5XFAD mice were significantly reversed by the administration of Theracurmin. Moreover, we observed that Theracurmin administration elevated the expression levels of synaptic components, including synaptophysin and post synaptic density protein 95, and decreased the expression levels of ionized calcium-binding adapter molecule 1 (Iba-1), a marker of activated microglia. These results suggest that Theracurmin ameliorates cognitive function by increasing the expression of synaptic components and by preventing neuronal cell damage from oxidative stress or from the activation of microglia. Thus, Theracurmin would be useful for treating the cognitive dysfunctions observed in AD.

• Journal of the Korean Society of Earth Science Education
• /
• v.12 no.1
• /
• pp.27-39
• /
• 2019

The purpose of this study is to develop a STEAM program for teaching climate change through CLAMP (Climate-Leaf Analysis Multivariate Program) paleoclimate inquiry in connection with high school 'Integrated Science' subject. In order to do so, we analyzed the 2015 revised national curriculum and science textbook in terms of the PDIE instructional design model, and developed the teaching-learning materials for 10 class hours through expert panel discussion and pilot test. According to the STEAM class procedure, in the situation presentation stage, the fossil leaves were collected from the dicotyledon plants near school, and the LMA (Leaf Margin Analysis) climate inquiry activity. was presented as the learning goal. During the creative design stage, students were taught about geology and leaf fossils in the study region, and CLAMP input data (31 characteristics of morphotype and leaf architectural of fossil leaves) were given. In the emotional experience and new challenge stage, we collected leaf fossils for outdoor learning, explored paleoclimate with CLAMP method, and promoted climatic literacy in the process of discussing tendencies and causes of Cenozoic's climate change. The validity of the development program was assessed (CVI .84) as being suitable for development purpose in all items through the process of establishing reliability among expert panel. In order to apply the program to the high school, a pilot test was conducted to supplement the discrepancies and to review the suitability. The satisfaction rate of the participants was 4.48, and the program was complemented with their opinions. This study will enable high school students to have practical knowledge and reacting volition for climate change, and contribute to fostering students' climate literacy.

• Journal of Broadcast Engineering
• /
• v.23 no.6
• /
• pp.855-865
• /
• 2018

Sound event detection is one of the research areas to model human auditory cognitive characteristics by recognizing events in an environment with multiple acoustic events and determining the onset and offset time for each event. DCASE, a research group on acoustic scene classification and sound event detection, is proceeding challenges to encourage participation of researchers and to activate sound event detection research. However, the size of the dataset provided by the DCASE Challenge is relatively small compared to ImageNet, which is a representative dataset for visual object recognition, and there are not many open sources for the acoustic dataset. In this study, the sound events that can occur in indoor and outdoor are collected on a larger scale and annotated for dataset construction. Furthermore, to improve the performance of the sound event detection task, we developed a dual CNN structured sound event detection system by adding a supplementary neural network to a convolutional neural network to determine the presence of sound events. Finally, we conducted a comparative experiment with both baseline systems of the DCASE 2016 and 2017.

• The Journal of the Korea Contents Association
• /
• v.19 no.6
• /
• pp.10-23
• /
• 2019

Although social big data can provide a multi-faceted perspective on school bullying experiences among children and adolescents, the complexity and variety of unstructured text presents a challenge for systematic collection and analysis of the data. Development of an ontology, which identifies key terms and their intricate relationships, is crucial for extracting key concepts and effectively collecting data. The current study elaborated on the definition of an ontology, carefully described the 7 stage development process, and applied the ontology for collecting and analyzing school bullying social big data. As a result, approximately 2,400 key terms were extracted in top-, middle-, and lower-level categories, concerning domains of participants, causes, types, location, region, and intervention. The study contributes to the literature by explaining the ontology development process and proposing a novel alternative research model that uses social big data in school bullying research. Findings from this ontology study may provide a basis for social big data research. Practical implications of this study lie in not only helping to understand the experience of school bullying participants, but also in offering a macro perspective on school bullying as a social phenomenon.