• Journal of Acupuncture Research
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• v.15 no.2
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• pp.227-236
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• 1998

This study dealed with the effects of the blood pressure and regional cerebral blood fIow(rCBF) on acupuncture and moxibustion treatment to the acu-point equivalent to K 1 of sprague dawley rats(SDR). Acupuncture treatment of K 1 significantly decreased BP in SDR. 2. Acupuncture treatment of K 1 significantly increased rCBF in SDR. 3. Moxibustion treatment of K 1 significantly increased BP in SDR. 4. Moxibustion treatment of K 1 significantly increased rCBF in SDR. These results suggest that acupuncture and moxibustion causes a diverse response of blood pressure and reginal cerebral blood flow. During the moxibustion treatment of K 1 increased BP and rCBF, but after moxibustion recorved BP and rCBF. During the acupunture tretment of K 1 decreased BP and then recorved, rCBF was significantly increased.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.1
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• pp.99-103
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• 2002

Geopungdodam-tang(GDT) have been used in oriental medicine for many centuries as a therapeutic agent of apoplexy. The mechanism of GDT on the cerebral blood flow is not known. The purpose of this Study was to investigate effects of GDT on the pial arterial diameter and action mechanism of GDT-induced increased regional cerebral blood flow(rCBF). The changes of regional cerebral blood f1ow(rCBF) was determinated by Laser-Doppler Flowmetry(LDF), and the changes of pial arterial diametet were determinated by video microscopy methods and video analyzer. The results were as follows ; 1. Pial arterial diameter was significantly increased by GDT in a dose-dependent manner. 2. Pretreatment with L-NNA significantly inhibited GDT induced increased rCBF. 3. Pretreatment with methylene blue significantly inhibited GDT induced increased rCBF. 4. Pretreatment with indomethacin inhibited GDT induced increased rCBF. These results suggest that GDT causes a diverse response of cerebral hemodynamics(rCBF and pial arterial diameter). The cerebral hemodynamics is also mediated by nitric oxide synthase, cyclic GMP(guanylyl cydase) and prostaglandin(cyclooxygenase).

• The Journal of Internal Korean Medicine
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• v.20 no.1
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• pp.153-166
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• 1999

SAENGMAEGSAN(SMS) has been used in oriental medicine for many years as a therapeutic agent for cerebral disease. The effect of SMS on the vascular system is not known. The purpose of this study was to determine the effect of SMS on blood pressure, regio-nal cerebral blood flow(rCBF). 1. Blood pressure did not change by SMS in rats. 2. rCBF was increased by SMS in a dose-dependent manner. 3. Pretreatment with propranolol. methylene blue and indomethacin significantly inhibited SMS induced increase in rCBF. These results suggest that SMS causes a diverse response of blood pressure, regional cerebral blood flow (rCBF) and pial arterial diameter. The increase in rCBF is also mediated by prostaglandins. cyclic GMP and adrenergic ${\beta}$receptor.

• The Journal of Internal Korean Medicine
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• v.26 no.3
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• pp.596-604
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• 2005

Objectives : Geopungjeseub-tang(Gufengchushi-tang) has been used in oriental medicine for many centuries as a therapeutic agent for hemiplegia caused by deficiency of qi(氣虛) and damp phlegm(濕痰). This study was performed to evaluate effects of Geopungjeseub-tang extract(GJT) on hemodynamics[regional cerebral blood flow(rCBF), pial arterial diameter(PAD), mean arterial blood pressure(MABP), heart rate(HR)] in normal rats and in rats with cerebral ischemia by middle cerebral artery(MCA) occlusion. Also, effects of adrenergic ${\beta}-receptor$, cyclooxygenase on response to GJT were evaluated. Methods : Laser-doppler flowmetry(LDF) measured changes of rCBF, MABP and HR. Video microscope and width analyzer measured changes in PAD. Results : rCBF and PAD increased after treatment with GJT(10mg/kg, i.v.) during the period of cerebral reperfusion, and pretreatment with indomethacin raised rCBF and PAD increased after treatment with GJT during the same period as above. Pretreatment with propranolol decreased rCBF, but increased after GJT treatment, but raised PAD increased after GJT treatment during this period of reperfusion. Conclusion : CR caused diverse responses were observed in rCBF and PAD after treatment with GJT. ACF action is mediated by adrenergic ${\beta}-receptor$ and cyclooxygenase. Result suggest that GJT has an anti-ischemic effect through the improvement of cerebral hemodynamics and has theraputic potential for cerebral apoplexy.

• Herbal Formula Science
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• v.7 no.1
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• pp.121-130
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• 1999

Oriental medicine prescription has been used for the treatment of various clinical symptoms associated with cerebral apoplexy. However, single herb drug does not used cerebral apoplexy and there mechanisms of action have not been defined, and it is not yet known what effects they have on the hemodynamics of cerebral circulation. The study was aimed to investigate the effect batryticatus bombycis(BB) on the vascular systems including changes in blood pressure (BP), and regional cerebral blood flow (rCBF) and of male Sprague-Dawely rats. The changes in rCBF were determinated by laser-Doppler flowmetry. 1. Blood pressure was not affected by BB in rats. 2. rCBF was increased by BB in a dose-dependent manner. 3. Pretreatment with methylene blue(1mg/kg), and propranolol(1mg/kg) did not inhibited BB induced increased in rCBF. 4. Pretreatment with propranolol(1mg/kg) was increased by BB in a dose-dependent manner in blood perssure. 5. Pretreatment with ODQ($10{\mu}g/kg$) and L-NNA(1mg/kg) were inhibited BB induced increased in rCBF. 6. Pretreatment with L-NNA(1mg/kg) was increased rCBF in a dose-dependent manner. These results suggest that BB causes a diverse response of blood pressure and regional cerebral blood flow(rCBF). The increased in rCBF is also mediated by nitric oxide synthease and guanylate cyclase.

• Herbal Formula Science
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• v.10 no.1
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• pp.105-112
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• 2002

Yongyanggaktang has been used in oriental medicine as a therapeutic agent for cerebral disease and eclampsia for many years. The purpose of this study was to determine the effect of Yongyanggaktang and its exception of Cornu Saigae Tataricae(羚羊角) in YYG about regional cerebral blood flow(rCBF) in rats. 1. rCBF was increased by Yongyanggaktang in dose dependently. 2. rCBF was increased by exception of Cornu Saigae Tataricae(羚羊角) in YYG at high dosages. 3. rCBF was decreased by Yongyanggaktang pretreated propranolol. 4. L-NNA did not affect rCBF. 5.ODQ did not affect rCBF. Above the results. Yongyanggaktang relates to the symphathetic action.

• The Journal of Internal Korean Medicine
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• v.30 no.2
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• pp.298-305
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• 2009

Objectives: The present study was carried out to investigate the effects of Yupoongyangyeong-tang(YYT) on changes of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in rats. In addition, the author also investigated action mechanisms of YYT on changes in rCBF and MABP. Methods and Results : In this study, treatment with YYT elevated rCBF in a dose-dependent manner, but MABP levels were elevated only in the 10 mg/ml treatment group. Pre-treatment with indomethacin. an inhibitor of cyclooxygenase, inhibited increase of rCBF effectively. Pre-treatment with methylene blue, an inhibitor of guanylate cyclase, inhibited increase of rCBF induced by YYT, too. In addition. pre-treatment with indomethacin also inhibited increase of MABP. However, pre-treatment with methylene blue did not affect MABP levels. Conclusion : These results suggest that YYT is useful to treat patients with diseases related to cerebral ischemia, because YYT can increase rCBF. In addition, the mechanisms are thought to be related to both cyclooxygenase and guanylate cyclase.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.2
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• pp.173-182
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• 2013

This Study was designed to investigate the effects of Sibjeondaebo-tang (SDT) and Gamy-Sibjeondaebo-tang (GST, Sibjeondaebo-tang adding Cervi Pantotrichum Cornu) on the improvement in regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, and in the rats with cerebral ischemia induced by middle cerebral artery occlusion, and further to determine the mechanisms. And, It was to investigate the effects of the SDT and GST with the change of histologic examination through the BDNF in the hippocampus CA1. In changes of cerebral hemodynamics, SDT and GST significantly increased rCBF in a dose-dependent manner but decreased MABP in normal rats. In mechanism of cerebral hemodynamics, Increase of GST-induced rCBF was significantly inhibited by pretreatment with methylene blue (0.01 mg/kg, i.p.), an inhibitor of guanylate cyclase, and Decrease of GST-induced MABP was significantly increased by pretreatment with methylene. These results suggested that the action of GST was mediated by guantlate cyclase pathway. In cerebral ischemics, the rCBF was stably improved by SDT (10 mg/kg, i.p.) significantly and stably increased by GST (10 mg/kg, i.p.) during the period of cerebral reperfusion, which contrast with the findings of rapid and marked increase in Control group. These results suggested that GST had anti-ischemic action in cerebral ischemic state. In histological examination through TTC stain, Sample A group and Sample B group decreased discoloration in the cortical part at $28^{th}$ day after MCAO induction. In immunohistochemistric response of BDNF, Sample A group and Sample B group increased respondent effect at $28^{th}$ day after MCAO induction. These results suggest that GST can Increase rCBF in normal state, as well as improve the stability of rCBF in cerebral ischemic state. Furthermore, methylene blue inhibitor study suggested the mechanism of blood flow enhancement by GST may be mediated by guanylate cyclase pathway.

• Journal of Acupuncture Research
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• v.28 no.4
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• pp.19-35
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• 2011

Objectives : This study was designed to investigate the effects of acupuncturing $PC_8$ used perpendicular needling method determine the mechanism of action of acupuncturing $PC_8$ by measuring the changes of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats. Methods : This study also investigated the effects of acupuncturing $PC_8$ on the change of rCBF in cerebral ischemic rats, and revealed the mechanism of its action. In addition, the effects of acupuncturing $PC_8$ on focal ischemic brain injury was studied in cerebral ischemic rats. Results : 1. Acupuncturing $PC_8$ significantly increase rCBF but decreased MABP in normal rats. 2. Acupuncturing $PC_8$ increased of rCBF was significantly inhibited by pretreatment with indomethacin (1mg/kg, i.p.), an inhibitor of cyclooxygenase in normal rats. 3. Acupuncturing $PC_8$ increased of rCBF was significantly inhibited by pretreatment methylene blue (10 ${\mu}g$/kg, i.p.), an inhibitor of guanylate cyclase in normal rats. 4. Acupuncturing $PC_8$ was significantly improved the rCBF than control group increased unstable in cerebral ischemic rats. 5. Acupuncturing $PC_8$ was not significantly improved the rCBF than control group by pretreatment with indomethacin (1mg/kg, i.p.), an inhibitor of cyclooxygenase in cerebral ischemic rats. 6. Acupuncturing $PC_8$ was significantly increased the rCBF than control group by pretreatment methylene blue ($10{\mu}g$/kg, i.p.), an inhibitor of guanylate cyclase in cerebral ischemic rats. Conclusions : In conclusion, our study suggested that acupuncturing $PC_8$ can increase rCBF in normal state, and improve stability of rCBF in ischemic state. In addition, we suggested that mechanisms related with acupuncturing $PC_8$ was involved in the guanylate cyclase pathway.

• Herbal Formula Science
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• v.6 no.1
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• pp.187-197
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• 1998

The study was aimed to investigate the effect cuscutae semen(CS) on the vascular systems including changes in blood pressure (BP), regional cerebral blood flow(rCBF) and pial arteriolar diameter of male Sprague-Dawely rats. The changes in rCBF were determinated by laser-Doppler flowmetry, and the changes in diameter of pial arteriole were measured through a closed crainal window. 1. Blood pressure was not affected by CS in rats. 2. rCBF was increased by CS in a dose-dependent manner. 3. Pretreatment with methylene blue(Img/kg), and propranolol(1mg/kg) significantly inhibited CS induced increased in rCBF. 4. Pretreatment with indomethacin(1mg/kg) did not inhibited CS induced increased in rCBF. 5. Pial arterial diameter was increased by CS in a dose-dependent manner. These results suggest that CS causes a diverse response of blood pressure, regional cerebral blood flow(rCBF), and pial arteral diameter. The increased in rCBF is also mediated by adrenergic ${\beta}-receptor $ and guanylate cyclase.