• Journal of Chest Surgery
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• 제45권3호
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• pp.196-198
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• 2012

Granular cell tumors are uncommon soft tissue neoplasm of nerve sheath origin, which are predominately benign. Granular cells can be found at any site in the body including the tongue, skin, subcutaneous tissue, breast, gastrointestinal, and urogenital systems. However, granular cell tumors have only been rarely described in the chest wall. Here we report a case of a granular cell tumor that occurred in the chest wall of a 59-year-old woman, along with a review of the literature.

• 대한치과의사협회지
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• 제55권12호
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• pp.828-840
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• 2017

In recent years, many researchers and clinicians found interest in regenerative medicine using induced pluripotent stem cells (iPSCs) with biomaterials due to their pluripotency, which is able to differentiate into any type of cells without human embryo, which of use is ethically controversial. However, there are limitations to make iPSCs from adult somatic cells due to their low stemness and donor site morbidity. Recently, to overcome above drawbacks, dental tissue-derived iPSCs have been highlighted as a type of alternative sources for their high stemness, easy gathering, and their complex (ectomesenchymal) origin, which easily differentiate them to various cell types for nerve, vessel, and other dental tissue regeneration. In other part, utilizing biomaterials for regenerative medicine using cell is recently highlighted because they can modulate cell adhesion, proliferation and (de)differentiation. Therefore, this paper will convey the overview of advantages and drawbacks of dental tissue-derived iPSCs and their future application with biomaterials.

• 약학회지
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• 제41권2호
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• pp.260-267
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• 1997

To establish prediabetes in vitro model concerning the etiology of IDDM(Insulin Dependent Diabetes Mellitus) in cellular level we have designed prediabetes in vitro models in pa ncreatic beta cells. HIT-T15, RINm5F and isolated rat islets were chosen as pancreatic beta cells, and streptozotocin (STZ) used as diabetogenic agent. Degree of beta cell destruction to establish prediabetic in vitro model was determined by cell proliferation and insulin release using thymidine uptake and radio immuno assay. When HIT-T15 and RINm5F cells were treated with STZ, the degree of cell deterioration was dependent upon the origin and passage number of beta cells, and in the case of isolated islets STZ showed the more sensitivity than above two beta cell lines. The concentration and exposure time of STZ treatment to establish prediabetes in vitro model in beta cell lines and isolated rat islets were 2 ~ 10mM, 30 min. and 1 ~ 5mM, 30 min., respectively.

• 대한수의학회지
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• 제13권1호
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• pp.67-73
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• 1973

Toxoplasma gondii (Tp), RH strain, was inoculated into cultured buffy coat cells obtained from the swine blood. The main reason for adopting swine lies in the animal's unusual susceptibility to Tp, As for the culture method used in the experiment, those well proved methods practised by Cho, Merchant, Moore and Tarnvik were mainly referred to as a starting point: hence, the author's method has been turned out to be the modified or supplementary form of those methods. Observations were made on the phase of multiplication of Tp in the cytoplasm. The results obtained were as follows: 1. Better growth and multiplication of Toxoplasma gondii were noticeably observed in the swine buffy coat cell, inoculated after three-to-five day cultivation of the cell. 2. In the lapse of the observation period, there appeard Toxoplasma gondii rarely available in the earlier stage, which had been inoculated into the cell after three-to-five day cultivation. In other words, Toxoplasma gondii started to show itself in seven or eight hours after inoculation, most outstandingly noticeable between twenty four hours and forty eight hours. Thereafter the disintegration stage of Toxoplasma gondii was observed.

• 한국태양에너지학회 논문집
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• 제39권1호
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• pp.1-9
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• 2019

Light induced degradation is one of the major research challenges of hydrogenated amorphous silicon related thin film silicon solar cells. Amorphous silicon shows creation of metastable defect states, originating from elevated concentration of dangling bonds during light exposure. The metastable defect states work as recombination centers, and mostly affects quality of intrinsic layer in solar cells. In this paper we present results of light induced degradation in thin film silicon solar cells and discussion on physical origin, mechanism and practical solutions of light induced degradation in thin film silicon solar cells. In-situ light-soaking IV measurement techniques are presented. We also present thin film silicon material with silicon nano-quantum dots embedded within amorphous matrix, which shows superior stability during light-soaking. Our results suggest that solar cell using silicon nano-quantum dots in abosrber layer shows superior stability under light soaking, compared to the conventional amorphous silicon solar cell.

• 전자공학회논문지D
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• 제36D권10호
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• pp.1-8
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• 1999

본 논문은 두께가 각각 다른 습식 산호막의 정전류 스트레스에 따른 SILC를 측정하여 SILC의 전도 mechanism 및 발생원인을 조사하였다. $N_2O$ 어닐링한 산화막의 SILC 특성도 조사하였다. 또한, 60A 두께의 $N_2O$ 어닐링한 터널링 산호막을 갖는 ,flash memory cell을 $0.25{\mu}m$ 설계규칙에 따라 제작하여 그 특성을 측정하였다. 그 결과, SILC의 발생 원인은 전기적 스트레스 인가에 따른 산호막내에 생성된 트랩 때문이며, SILC의 전도 mechanism은 전기장 세기가 8MV/cm 이하일 때 산호막 트랩을 경유한 modified F-N 터널링이 8MV/cm 이상일 때 전형적인 F-N터널링이 주도적임을 알 수 있었다. 60A의 $N_2O$ 어닐링한 산화막은 SILC에 대한 내성 측면에서 큰 개선 효과가 있음을 알 수 있었다. 또한 이 막을 flash memory cell의 터널링 산호막으로 이용할 경우, $10^6$회의 endurance와 10년 이상의 드레인 disturb가 보장되고 8V-프로그래밍이 가능한 특성을 얻을 수 있었다.

• Clinics in Shoulder and Elbow
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• 제15권2호
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• pp.65-72
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• 2012

목적: 세가지 기원의 줄기 세포 분화능과 면역표현형을 평가하고자 하였다. 대상 및 방법: 견봉하 점액낭과 골수, 탯줄 혈액 세 개의 군에서 세포를 채취하였다. 견봉하 점액낭과 골수는 견관절 수술 환자군에게 임상적 동의 하에 수술중 채취하였다. 각각의 채취된 세포 및 탯줄 혈액에 대하여 계대 배양을 시행하여 신경 분화군, 지방 분화군, 골 분화군을 평가하였으며 세포 표면 항체를 밝히기 위해 유동세포분석법을 이용하였다. 결과: 견봉하 점액낭 유래 세포에서는 신경분화와 지방 분화는 8예 모두 (100%)에서, 골분화는 8례 중 5예 (62.5%)에서 성공할 수 있었으며 골수 유래 세포의 경우 신경 및 지방 분화 유도한 6례 및 5예 모두 (100%) 분화에 성공하였으나 골분화 유도는 5예 중 4예 (80%)에서 얻을 수 있었다. 반면 탯줄 유래 세포 분화 연구의 경우 신경 분화 유도 67례 중 65예 (97%)에서 지방 분화 연구 54예 중 29예 (53.7%)에서 골 분화 연구 57예 중 39예 (68.4%)에서 성공할 수 있었다. 결론: 탯줄 유래 줄기세포의 분화능과 비교하였을 때 견봉하 점액낭 및 골수 유래 줄기세포의 분화능이 우수함을 알 수 있으며 이는 향후 세포 치료에 있어서 안정성 있는 치료 제공자가 될 수 있을 것으로 보이며 향후 생체 실험 연구의 참고 자료로서도 가치가 있을 것으로 보인다.

• 대한의용생체공학회:의공학회지
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• 제16권3호
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• pp.331-336
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• 1995

체내 매입후 경시적으로 분해되면서 재생골조직에 의해 치환되는 인공골복합체를 제조하고 복합체가 세포활성에 미치는 영향을 조사하였다. 복합체시편을 세포배양액에 넣고 1주일동안 $37^{\circ}C$에서 배양한 다음, 사람자궁경부암유래 HeLa S3세포와 쥐피하 L929세포를 복합체가 용해된 세포배양액에서 5일간 배양하여 세포성장율을 비교하여 세포특성을 조사하였다. 한편 HeLa S3세포를 배양중인 배양액에 ${Na_2}^{51}CrO_4$를 첨가하여 HeLa S3세포에 $^{51}Cr$를 표식한 다음, 용해된 $^{51}Cr$의 양을 $\gamma$-counter를 이용하여 측정하였다. 세포성장정도의 측정에서는 HeLa S3 세포 및 L929 세포 모두가 특이한 세포독성을 발견할 수 없었으며, 복합체가 용해된 세포배양액내의 표식된 HeLa S3 세포로 부터 용해된 $^{51}Cr$량을 측정한 결과, 세포활성을 저해하지 않은 것으로 관찰되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6047-6053
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• 2012

Bracken fern [Pteridium aquilinem (L.) kuhn (Dennstaedtiaceae)] is one of the most common species on the planet. It has been consumed by humans and animals for centuries. Use by some human groups is because they believe bracken fern is good for health as plant medicine. However, it is also one of the few known plants that can cause tumors in farm animals. Many interested groups have focused their attention on bracken fern because of these interesting features. In order to evaluate the biological effects of exposure to this plant in cellular level, human cancer cell lines were treated with the fern dichloromethane extracts and the genotoxic and cytotoxic effects were studied. Anti-proliferative/cytotoxic effects were evaluated by cell count, MTT assay and flow cytometry methods with three different cancer cell lines, TCC, NTERA2, and MCF-7, and two normal cells, HDF1 and HFF3. Pro-apoptotic effects of the extracts were determined by DAPI staining and comet assay, on TCC cancer cells compared to the normal control cell lines. Cellular morphology was examined by light microscopy. Our present study showed that the extract caused DNA damage and apoptosis at high concentrations ($200{\mu}g/mL$) and also it may induce cell cycle arrest (G2/M phase) at mild concentrations (50 and $30{\mu}g/mL$) depending on the cell type and tumor origin. These results indicate that bracken fern extract is a potent source of anticancer compounds that could be utilized pharmaceutically.

• Asian Pacific Journal of Cancer Prevention
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• 제17권6호
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• pp.2909-2916
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• 2016

Background: Canine malignant lymphoma is classified into B- or T-cell origin, as in the human case. Due to differences in prognosis, a suitable method needs to be developed for lineage identification. Aims: To determine the accuracy of immunophenotypic and molecular information between three methods: immunocytochemistry (ICC), immunohistochemistry (IHC) and heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) in spontaneous canine lymphomas. Materials and Methods: Peripheral blood, fine needle aspiration and tissue biopsies from enlarged peripheral lymph nodes prior to treatment of 28 multicentric lymphoma patients were collected. Cytopathology and histopathology were examined and classified using the updated Kiel and WHO classifications, respectively. Anti-Pax5 and anti-CD3 antibodies as B- and T-cell markers were applied for immunophenotyping by ICC and IHC. Neoplastic lymphocytes from lymph node and white blood cell pellets from peripheral blood were evaluated by hPARR. Results: In this study, low grade B-cell lymphoma accounted for 25% (7/28), high grade B-cell lymphoma for 64.3% (18/28) and high grade T-cell lymphoma for 10.7% (3/28). According to the WHO classification, 50% of all cases were classified as diffuse large B-cell lymphoma. In addition, ICC showed concordant results with IHC; all B-cell lymphomas showed Pax5+/CD3, and all T-cell lymphomas exhibited Pax5-/CD3+. In contrast to hPARR, 12 B-cell lymphomas featured the IgH gene; seven presented the $TCR{\gamma}$ gene; five cases showed both IgH and $TCR{\gamma}$ genes, and one case were indeterminate. Three T-cell lymphomas showed the $TCR{\gamma}$ gene. The percentage agreement between hPARR and ICC/IHC was 60%. Conclusions: Immunophenotyping should not rely on a single method. ICC or IHC with hPARR should be used concurrently for immunophenotypic diagnosis in canine lymphomas.