• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.76-76
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• 2001

The objective of this study was to optimize the vitrification method of in vitro produced bovine embryos. Thus, in vitro produced embryos at 8 cell, morula and blastocyst stages were vitrified on electron microscope grids (EM grids) or in open pulled straws (OPS) with EG5.5 (5.5 M ethylene glycol, 1.0 M sucrose and 10% FBS in m-DPBS medium) freezing solution and their survival rates after thawing were compared. The embryos on EM grids or in OPS were briefly exposed to EG5.5 freezing solution and plunged directly into liquid nitrogen within 30 to 35 sec. Post-thawed embryos were serially diluted in 0.5, 0.25 and 0.125 M sucrose in m-DPBS, each for 1 min, and then cultured in CRI aa medium supplemented with 10% FBS. Embryonic survival rate was assessed as re-expanded and hatched rates of those embryos after warming. The rates of re-expansion embryos did not significantly different between EM grid (8 cell: 42.10%, morula: 66.66% and blastocyst: 77.08%) and OPS (8 cell: 47.36%, morula: 61.90% and blastocyst: 83.33%) methods. In addition, the hatched rates in EM grid (8 cell: 31.57%, morula: 57.14% and blastocyst: 72.91%) were similar to those in OPS (8 cell. 34.21%, morula: 50.00% and Blastocyst: 77.08%). Interestingly, even at the same blastocyst stage, the in vitro survival of day 7 embryos (EM grid: 79.48 and OPS: 87.18%) was higher than those of day 8 embryos (EM grid: 72.10 and OPS: 82.06%). The total cell number of blastocyst developed in vitro after vitrification was examined with Hoechst 33342 staining to compare the embryo quality among different treatment groups. The total cell number of blastocyst was not significantly different between vitrified groups (EM grid: 162.4$\pm$8.0 and OPS: 158.4$\pm$7.1) and unvitrified control (168.0$\pm$5.6). These results indicate that both vitrification containers can provide the high rate of embryo survival. Moreover, the OPS container may not need a cap to protect the container from floating after immersion in L$N_2$. Therefore, this study suggest that bovine embryos can be cryopreserved easily, effectively and successfully by vitrification method using EM grid or OPS with EG5.5 freezing solution. In the future, the Pregnancy rate would be investigated after transfer of our vitrified embryos into the appropriated recipients.

• 대한기계학회논문집B
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• 제29권9호
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• pp.1049-1056
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• 2005

A conservative pressure-based finite-volume numerical method has been developed for computing flow and heat transfer by using an unstructured grid system. The method admits arbitrary convex polyhedra. Care is taken in the discretization and solution procedures to avoid formulations that are cell-shape-specific. A collocated variable arrangement formulation is developed, i.e. all dependent variables such as pressure and velocity are stored at cell centers. Gradients required for the evaluation of diffusion fluxes and for second-order-accurate convective operators are found by a novel second-order accurate spatial discretization. Momentum interpolation is used to prevent pressure checkerboarding and the SIMPLE algorithm is used for pressure-velocity coupling. The resulting set of coupled nonlinear algebraic equations is solved by employing a segregated approach, leading to a decoupled set of linear algebraic equations fer each dependent variable, with a sparse diagonally dominant coefficient matrix. These equations are solved by an iterative preconditioned conjugate gradient solver which retains the sparsity of the coefficient matrix, thus achieving a very efficient use of computer resources.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2009년 추계학술대회논문집
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• pp.201-210
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• 2009

A volume capturing method using unstructured grid system for numerical analysis of multiphase flows is introduced in the present paper. This method uses an interface capturing method (CICSAM) in a volume of fluid(VOF) scheme for phase interface capturing. The novelty of CICSAM lies in the adaptive combination of high resolution discretization scheme which ensures the preservation of the sharpness and shape of the interface while retaining boundedness of the field, and no explicit interface reconstruction which is perceived to be difficult to implement on unstructured grid system. Several typical test cases for multiphase flows are presented, which are simulated by an in-house solution code(PowerCFD). This code employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with CICSAM. It is found that the present method simulates efficiently and accurately complex free surface flows such as multiphase flows.

• 지구물리와물리탐사
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• 제6권2호
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• pp.77-86
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• 2003

자유면 경계조건을 정착하게 묘사할 수 있는 변위근사 유한차분법을 이용하는 시간영역 탄성파 모델링법을 고안하였다. 기존의 변위근사 유한차분법의 경우 변위와 매질의 물성을 격자점에 정의하는 격자군(격자점 기반의 격자군)을 이용하였으나, 이 연구에서 제시하는 새로운 유한차분법에서는 변위는 격자점에 정의하지만 매질의 물성을 격자점으로 둘러싸인 면에 정의하는 격자군(셀 기반의 격자군)을 이용한다. 매질의 물성을 셀에 정의할 경우 자유면에서 응력이 사라진다는 자유면 경계조건을 추가로 적용할 필요가 없으며 매질의 물성 변화만으로 자유면 경계조건을 표현할 수 있다. 수치예를 통한 정확도 분석 결과 셀 기반의 격자군을 이용할 경우 계산된 수치석인 해가 해석적인 해에 매우 근사함을 알 수 있었다.

• 한국태양에너지학회 논문집
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• 제33권6호
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• pp.92-97
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• 2013

This paper proposes DC offset current compensation method of transformerless fuel cell/PV PCS. DC offset current is generated by the unbalanced internal resistance of the switching devices in full bridge topology. The other cause is the sensitivity of the current sensor, which is lower than DSP in resolution. If power converter system has these causes, the AC output current in the inverter will generate the DC offset. In case of transformerless grid-connected inverter system, DC offset current is fatal to grid-side, which results in saturating grid side transformer. Several simulation results show the difficulties of detecting DC offset current. Detecting DC offset current method consists of the differential amplifiers and PWM is compensated by the output of the Op amp circuit with integrator controller. PSIM simulation verifies that the proposed method is simpler and more effective than using low resolution current sensor alone.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2004년도 추계 학술대회논문집
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• pp.83-86
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• 2004

In this paper an automated hole-finding method for overset grids is introduced which uses recursive octree-cell division. A graphic program which enables the user to do the hole-cutting with ease is also introduced. Using this program it was found that a proper combination of the level of octree division and vector calculation should be used for efficient and fast hole finding.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2002년도 학술대회지
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• pp.91-94
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• 2002

It is important to develop new effective technologies for increasing the interruption capacity and reducing the size of a GCB (Gas Circuit Breaker). It is not easy to test the real GCB model in practice as in theory. Therefore, a simulation tool based on a CFD (Computational Fluid Dynamics) algorithm has been developed to facilitate an optimization of the interrupter. But the choice of grid is not at all trivial in the complicated geometries like a GCB. In this paper, we have applied a CFD-CAD integration using Cartesian cut-cell method, which is one of the grid generation techniques for dealing with complex and multi-component geometries.

• 대한기계학회논문집
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• 제17권1호
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• pp.182-189
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• 1993

본 연구에서는 한곳에 적응효과가 중복되는 것을 피하고 해의 변화율이 상대 적으로 큰 곳에 대해 대등한 격자 적응효과를 주는 방법을 연구하였다. 전 유동장에 서 해의 변화율을 계산하여 하한값(threshold) 보다 큰 값을 갖는 cell에 대해 같은 크기의 가중함수(weight function) 값을 갖게 한다. 하한값(threshold)은 전체 cell 수에 대해 상위의 변화율을 갖는 cell의 백분율(percentage)로부터 구한다. 이 방법 은 하한값을 직접 대입해야 한다는 단점은 있으나 변황율이 상대적으로 큰 영역에 대 해 고른 격자 적응 효과를 줌으로 해서 격자 적응의 회수를 줄일 수 있으며 해의 발달 에 긍정적인 격자를 생성할 수 있다.

• 한국농공학회지
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• 제39권1호
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• pp.64-74
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• 1997

A CELTHYM(CEll-based Long-term HYdrologic Model), a pre-processor and a post-processor that can be integrated with geographic information system(GIS) were developed to predict the stream flow from the small agricultural watershed on the daily basis. The CELTHYM calculates the direct runoff from a grid using SCS curve number method and then sum up all of cells with respect to a sub-catchment area belonged to a stream grid and integrated to an outlet. Base flow of a watershed outlet was computed by integrating of the base flow of each stream grid that was averaged the sub-catchment deep-percolation and calculated with the release rate. Two kind of water budget equation were used to compute the water balance in a grid that was classified into not paddy field and paddy field. One of the two equation is a soil water balance equation to account the soil moisture of the upland, forest and excluding paddy field grid. The other is a paddy water balance equation for the paddy field, calculating the ponding depth, the effective rainfall, the deep percolation and the evapotranspiration.

• 한국전산유체공학회지
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• 제7권2호
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• pp.8-16
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• 2002

A numerical study on the use of the momentum interpolation method for flows with a large body force is presented. The inherent problems of the momentum interpolation method are discussed first. The origins of problems of the momentum interpolation methods are the validity of linear assumptions employed for the evaluation of the cell-face velocities, the enforcement of mass conservation for the cell-centered velocities and the specification of pressure and pressure correction at the boundary. Numerical experiments are performed for a typical flow involving a large body force. The numerical results are compared with those by the staggered grid method. The fact that the momentum interpolation method may result in physically unrealistic solutions is demonstrated. Numerical experiments changing the numerical grid have shown that a simple way of removing the physically unrealistic solution is a proper grid refinement where there is a large pressure gradient. An effective way of specifying the pressure and pressure correction at the boundary by a local mass conservation near the boundary is proposed, and it is shown that this method can effectively remove the inherent problem of the specification of pressure and pressure correction at the boundary when one uses the momentum interpolation method.